• Title/Summary/Keyword: RDA test

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Interpretation of Varietal Response to Rice Leaf Blast by G$\times$E Analysis with Reduced Number of Nursery Test Sites

  • Yang, Chang-Ihn;E. L. Javier;Won, Yong-Jae;Yang, Sae-Jun;Park, Hae-Chune;Shin, Young-Boum
    • KOREAN JOURNAL OF CROP SCIENCE
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    • v.45 no.5
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    • pp.316-321
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    • 2000
  • Blast severity data of 39 rice varieties at 11 sites in Korea from 1997 to 1999 were analyzed using AMMI model and pattern analysis. Genotype x Environment (G$\times$E) interaction sum of squares (SS) accounted for 12 % of the total SS. Eight genotype groups and seven location groups were identified based on blast reaction pattern. The data obtained from over 21 sites with 44 test varieties from 1981 to 1996 were also considered. These were compared with the 1997-1999 data using the G$\times$E analysis results. Majority of the variability in the Korean Rice Blast Nursery (KRBN) were attributable to variations due to genotypes. Variations of G$\times$E interaction were maintained though test sites were reduced from 21 to 11 sites. Broadly compatible biological discriminative varieties identified were Nagdongbyeo and Akibare while broadly incompatible biological discriminative varieties identified were Hangangchalbyeo and Seogwangbyeo. Key sites for future evaluation work could be selected from location groups. Each location group should be represented by the site with the strongest interaction pattern. Blast responses in Cheolwon, Gyehwa, Suwon, Iksan, and Icheon showed different patterns from other locations.

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In vitro Skin Irritation Test of Honeypolis using Human Skin Model

  • Woo, SoonOk;Han, Sangmi;Hong, Inpyo;Kim, Sung-kuk
    • Journal of Apiculture
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    • v.33 no.4
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    • pp.277-282
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    • 2018
  • Ethanol extracted propolis (EEP) was mixed with honey (honeypolis) to dissolve well in water and in vitro skin irritation test was conducted. In vitro method is designed to predict and classify the skin irritation potential of a chemical by assessment of its effect on $EpiDerm^{TM}$, a reconstituted three-dimensional human epidermis model. Cytotoxicity is expressed as the reduction of mitochondrial dehydrogenase activity measured by formazan production from MTT after a 60 min exposure period. In this study under the given conditions honeypolis showed no irritant effects. Honeypolis meets acceptance criteria if: mean absolute OD 570 nm of the three negative control tissues is ${\geq}0.8$ and ${\leq}2.8$, mean relative tissue viability of the three positive control tissues is ${\leq}20%$, standard deviation of relative tissue viability obtained from each three concurrently tested tissues is ${\leq}18%$. Honeypolis is therefore classified as "non-irritant" in accordance with UN GHS "No Category".

Effect of Soil Respiration on Light Fraction-C and N Availability in Soil Applied with Organic Matter

  • Ko, Byong-Gu;Lee, Chang-Hoon;Kim, Myung-Sook;Kim, Gun-Yeob;Park, Seong-Jin;Yun, Sun-Gang
    • Korean Journal of Soil Science and Fertilizer
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    • v.49 no.5
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    • pp.510-516
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    • 2016
  • Soil respiration has been recognized as a key factor of the change of organic matter and fertility due to the carbon and nitrogen mineralization. In this study, we evaluated the effect of soil respiration on the light fraction-C and inorganic N content depending on temperature in soil applied with organic matter. Soil respiration was calculated by using total $CO_2$ flux released from soil applied with $2Mg\;ha^{-1}$ of rice straw compost and rye for 8 weeks incubation at 15, 25, $35^{\circ}C$ under incubation test. After incubation test, light fraction and inorganic N content were investigated. Rye application dramatically increased soil respiration with increasing temperature. $Q_{10}$ value of rye application was 1.69, which was higher 27% than that of rice straw compost application. Light-C and $NO_3-N$ contents were negatively correlated to soil respiration. Light-C in rye application more decreased than that in rice straw compost with temperature levels. These results indicate that temperature sensitivity of soil respiration could affect soil organic mater content and N availability in soil due to carbon availability. Also, light fraction would be useful indicator to evaluate decomposition rate of organic matter in soil under a short-term test.

Effect of Chain Type Mole-Drainer Attached to Tractor for Drainage Improvement (배수개선을 위한 트랙터 부착식 체인형 암거 천공기의 적용 효과)

  • Jeon, Jonggil;Choi, Yonghun;Kim, Minyoung;Kim, Youngjin;Lee, Sangbong
    • Journal of The Korean Society of Agricultural Engineers
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    • v.62 no.4
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    • pp.63-73
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    • 2020
  • The area of paddy fields for upland crop cultivation is gradually increasing. In this context, we developed a chain type mole drainer that can be attached to a tractor to prevent the wet injury of crops. To conduct a field test, we formed underdrains at 2 m intervals in a paddy field wherein soybeans are cultivated, and we investigated the status of the soil moisture, groundwater level, and soybean growth during the cultivation period. The soil moisture content decreased by 22.3~26.8% in the test plot and by 5.9~6.9% in the control plot during a period of 57~88 hour after a rainfall of 41.5~157.0 mm. The effect of underdrainage was clear as the hourly groundwater level decreased approximately 2.8 times faster in the test plot compared with the control plot. Moreover, the soybean yield was greater by 78.6 kg/10a in the test plot than in the control plot. Therefore, for soybean cultivation in paddy fields, the use of the developed mole drainer is expected to help in improving the drainage and increasing the soybean yield.

Comparison of Toxic Response of Cladocerans to Organic Solvents to Establish the Standard Test Guidelines Using Korean Native Species (한국산 물벼룩 표준생태독성시험법 확립을 위한 10종 용매대조물질에 대한 독성반응 비교)

  • Kim, Byung-Seok;Park, Yoen-Ki;Yang, Yu-Jung;Hong, Soon-Sung;Park, Kyung-Hun;Jeong, Mi-Hye;Kim, Se-Ri;Park, Kyeong-Hun;Yeh, Wan-Hae;Kim, Doo-Ho;Yun, Jong-Chul;Hong, Moo-Ki;Kyung, Kee-Sung;Ahn, Young-Joon
    • The Korean Journal of Pesticide Science
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    • v.14 no.1
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    • pp.10-15
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    • 2010
  • Most pesticides are poorly soluble in water and must be dissolved in a solvent carrier before being added to the test medium on aquatic toxicity test. The concentration of solvent is critical to the success of a test. This study were conducted to recommend possible organic solvents which have good solubility for pesticides and low toxicity to Korean native water flea to establish new standard toxicity test methods using Korean native water flea for ecological risk assessment of pesticide. Four Korean freshwater cladocerans, Daphnia obtusa, Daphnia sp., Moina macrocopa and Simocephalus vetulus were exposed to 10 different organic solvents during 48 hours to evaluate their toxic response to solvents. Ethyl acetate was the most toxic to cladocerans tested. Although ethyl ether was the least toxic to cladocerans tested, it may not adequate as possible solvent in aquatic toxicity test due to high volatility and low water solubility. In conclusion, acetone, methanol, ethanol and acetonitrile which has low toxicity as well as good water solubility are recommended as optimal organic solvent to use in aquatic toxicity tests with Korean native cladocerans tested.

New Mid-late Maturing F1 Hybrid Onion Cultivar, "Yeongpunghwang" (양파 추파형 중·만생 다수성 신품종 "영풍황")

  • Lee, Eul-Tai;Kim, Cheol-Woo;Choi, In-Hu;Jang, Young-Seok;Bang, Jin-Ki;Bae, Sang-Gyeong;Hyun, Dong-Yun;Jung, Jong-Mo;Ha, In-Jong;Kim, Seong-Bae
    • Korean Journal of Breeding Science
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    • v.41 no.4
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    • pp.587-590
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    • 2009
  • New onion hybrid cultivar, "Yeongpunghwang" was developed from cross between "MOS8", male sterile line and "Mokpo 11", pollen line by Mokpo Sub-Station, National Institute of Horticultural & Herbal Science, RDA in 2008. The crossing line was performed yield test in 2003. IT was evaluated as "Mokpo 11" in the regional yield trial test(RYT) from 2006 to 2008 during three years. This line is semi-erect plant growth type and has green leaf colour. It has broad ovate bulb shape and yellow skin colour in bulb characteristics. Plant height, leaf number, and bolting rate was 78cm, 7.9, and 3.9%, respectively. Lodging date as harvesting indicator was on 26 May. The yield potential of "Yeongpunghwang" was out-yielding 6% compare to check variety ("Sunpower") by with 8,344 kg/10a in RYT. It showed moderated cold hardness in all regional location which was conducting RYT, including Muan, Goheung, Changyeong and Andong. So this variety was recommend as a leading variety at southern area of korea.

PEGylation of Silk Fibroin Model Peptide

  • Kweon, Hae-Yong;Jo, You-Young;Yeo, Joo-Hong;Woo, Soon-Ok;Han, Sang-Mi;Lee, Kwang-Gill
    • International Journal of Industrial Entomology and Biomaterials
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    • v.20 no.2
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    • pp.87-91
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    • 2010
  • Silk fibroin model peptide, alanine pentamer was synthesized through solid-phase method and modified with poly(ethylene glycol). Nuclear magnetic resonance spectrometry and Fourier-transform infrared spectroscopy showed the conformation of alanine pentamer, $\beta$-sheet structure and random coil conformation were not changed with PEGylation. Differential scanning calorimetry showed that relatively strong exothermic peak around $180^{\circ}C$ by PEGylation. No cytotoxicity of PEGylated pentamer was observed by L929 cell proliferation test.

Mutagenecity evaluation of insecticidal 2-carbomethoxy-4-chlorodiethyl phosphate in short-term bioassays (살충성 물질 2-carbomethoxy-4-chlorodiethyl phosphate의 유전독성 평가)

  • Lee, Je-Bong;Sung, Ha-Jung;Jeong, Mi-Hye;Kwon, Oh-Kyung;Lee, Hae-Keun;Kim, Young-Koo
    • The Korean Journal of Pesticide Science
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    • v.2 no.2
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    • pp.53-58
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    • 1998
  • For evaluating the mutagenic potential of 2-carbomethoxy-4-chlorodiethyl phosphate, three different short-term mutagenicity tests were used; Salmonella typhimurium preincubation assay with and without rat liver microsomal activation, chromosome aberration test in cultured chinese hamster lung fibroblast cell and in vivo micronucleus test in male mice bone marrow. In Salmonella typhimurium reverse mutation assay using TA98, TA100, TAl535 and TAl537, 2-carbomethoxy-4-chlorodiethyl phosphate did not show any mutagenic response in the presence and absence of S9 mix. It did not induce any significant structural chromosome aberrations in the absence of metabolic activation. In micronucleus test using ICR mice, the frequency of micronucleated polychromatic erythrocytes (MNPCE) increased in bone marrow cells treated with positive control, mitomycin-C, but 2-carbomethoxy-4-chlorodiethyl phosphate did not increase micronucleated polychromatic erythrocytes. These results indicate that 2-carbomethoxy-4-chlorodiethyl phosphate does not show any positive responses in short-term genotoxicity assays.

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