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PEGylation of Silk Fibroin Model Peptide  

Kweon, Hae-Yong (Sericultural & Apicultural Materials Division, National Academy of Agricultural Science, RDA)
Jo, You-Young (Sericultural & Apicultural Materials Division, National Academy of Agricultural Science, RDA)
Yeo, Joo-Hong (Sericultural & Apicultural Materials Division, National Academy of Agricultural Science, RDA)
Woo, Soon-Ok (Sericultural & Apicultural Materials Division, National Academy of Agricultural Science, RDA)
Han, Sang-Mi (Sericultural & Apicultural Materials Division, National Academy of Agricultural Science, RDA)
Lee, Kwang-Gill (Sericultural & Apicultural Materials Division, National Academy of Agricultural Science, RDA)
Publication Information
International Journal of Industrial Entomology and Biomaterials / v.20, no.2, 2010 , pp. 87-91 More about this Journal
Abstract
Silk fibroin model peptide, alanine pentamer was synthesized through solid-phase method and modified with poly(ethylene glycol). Nuclear magnetic resonance spectrometry and Fourier-transform infrared spectroscopy showed the conformation of alanine pentamer, $\beta$-sheet structure and random coil conformation were not changed with PEGylation. Differential scanning calorimetry showed that relatively strong exothermic peak around $180^{\circ}C$ by PEGylation. No cytotoxicity of PEGylated pentamer was observed by L929 cell proliferation test.
Keywords
Silk fibroin; Alanine pentamer; Solid-phase peptide synthesis; Poly(ethylene glycol); Cytotoxicity;
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