• Title/Summary/Keyword: RDA test

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Rapid Detection Kit for Salmonella typhimurium (살모넬라 식중독균 신속 검출용 간이 진단키트)

  • Kim, Gi-Young;Yang, Gil-Mo;Park, Saet-Byeol;Kim, Yung-Hwun;Lee, Kang-Jin;Son, Jae-Yong;Kim, Hyuck-Joo;Lee, Sae-Rom
    • Journal of Biosystems Engineering
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    • v.36 no.2
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    • pp.140-146
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    • 2011
  • This study was performed to develop a rapid test kit for pathogenic Salmonella in various samples. The rapid detection kit has been fabricated based on nitrocellulose lateral-flow strip. Colloidal gold and biotin conjugated Salmonella antibodies were used as a tag and a receptor, respectively. Manually spotted Salmonella antibody and Neutravidin on nitrocellulose membrane were used as test and control lines, respectively. Feasibility of the rapid kit to detect Salmonella typhimurium in samples were evaluated. The intensity of the color of the test line started to increase with the samples in which higher concentration of the cells were contained. The sensitivity of the sensor was $10^6$ cfu/mL Salmonella spiked in PBS. Also, the rapid test kit could detect $10^6$ cfu/mL of Salmonella in chicken meat extract.

Effects of Number of Incomplete Data in Latest Generation on the Breeding Value Estimated by Random Regression Model (임의회귀 모형 사용시 마지막 세대의 불완전한 기록이 추정육종가에 미치는 효과)

  • ;;;;;;;;Salces, A.J.
    • Journal of Animal Science and Technology
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    • v.48 no.2
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    • pp.143-150
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    • 2006
  • The data were collected in the dairy herd improvement program from January 2000 to July 2005. Test data included 825,157 records of first parity and animals with both parents known were included. This study aimed to describe the effect of incomplete lactation records of latest generation to the change in sire's breeding value using Random Regression model (RRM) in genetic evaluation. Estimation of genetic parameter and breeding value for sire used REMLF90 and BLUPF90 program. The phenotypic value on the number of test day records between group TD11, TD8, TD5, TD2 showed no large differences. For all the group heritability of test day milk yield range from 0.30 to 0.36. However TD2 group showed low heritability the least test day recode on the latest generation. The correlation of above 50% between test day and TD11(0.610), TD8(0.616), TD5(0.661) and TD2(0.682) with different records in latest generation. Sire's rank of breeding value varied widely depending on the records on the number of lactation from start to the latest generation. Study showed that change in breeding value ranked if daughter's test recode more so it should have at least 5 test day records. The use of RRM in dairy cattle genetic evaluation would be desirable if complete lactation records for latest generation daughters of young bulls when selection for proven bulls. Random Regression model (RRM) require at least 5 test-day lactation recode.

Comparison of Antimicrobial Residue Detection in Goat Milk by the Delvo, Eclipse 100, and Parallux Tests (Delvo test, Eclipse 100 및 Parallux를 이용한 산양유의 잔류항균물질 검출 비교)

  • Ham, Jun-Sang;Jeong, Seok-Geun;Shin, Ji-Hye;Han, Gi-Sung;Chae, Hyun-Seok;Yoo, Young-Mo;Ahn, Jong-Nam;Hur, Tae-Young;Ko, Sang-Hyun;Park, Kwang-Wook;Choi, Seok-Ho;Lee, Wan-Kyu
    • Food Science of Animal Resources
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    • v.28 no.1
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    • pp.59-62
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    • 2008
  • The aim of this work was to assess the Delvo test for the detection of antimicrobial residues in goat milk. A total of fifty six samples (eight farms, seven samplings each) were analyzed by the Delvo, Eclipse 100, and Parallux tests. None of the samples showed positive results with the Parallux test which is based on immune-chemical methods. However, 37.5% of samples showed positive results with the Delvo test. 3.6% of samples showed positive results with the Eclipse 100 test, which is based on a microbiological method. The Delvo test is included in the 'standard methods for the examination of raw milk' by the National Veterinary Research and Quarantine Service as a microbiological method used for the detection of antimicrobial residues. Because "raw milk" is defined as 'milked state of cow, ewe and goat milk for sale or for processing' in the Animal Food Products Processing Law, the Delvo test should be excluded from the 'standard method for the examination of raw milk', or additional official documents referring to the Delvo test as not appropriate for the detection of antimicrobial residues of goat milk are required.

Assessment of Equine Temperament by a Questionnaire Survey (Questionnaire Survey를 이용한 말의 품성 평가)

  • Kim, Nam-Young;Park, Yong-Sang;Ko, Moon-Suck;Chae, Hyun-Seok;Cho, Won-Mo;Cho, In-Chul;Hong, Hyun-Ju;Park, Ji-Yun;Chung, Kwang-Yeoun;Cho, Sang-Rae
    • Journal of Embryo Transfer
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    • v.28 no.3
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    • pp.223-227
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    • 2013
  • The objective of the study is to assess temperament of a horse based on general temperament test by a questionnaire survey. Five test criteria were identified: gentleness, patience, aggressiveness, sensitivity, and friendliness, each on a 5-point scale. 114 horses bred at the National Institute of Animal Science (NIAS) in 2010, 2011 and 2012. The horses recorded scores of 3.6~3.9 for gentleness, 3.1~3.6 for patience, 3.4~4.0 for aggressiveness, 2.8~3.2 for sensitivity, and 3.4~3.8 for friendliness, the overall score for sensitivity the lowest. Horses born in 2012 scored lower than the rest in all five areas at a statistically significant level (P<0.05). By gender, the colts scored higher than the fillies in all five areas, but the discrepancy was not statistically significant. Factor analysis yielded only one factor, and the Cronbach's ${\alpha}$ value was 0.980 for standardization of Factor 1, indicating a high reliability of internal consistency. The correlation coefficients among the test criteria ranged between 0.85 and 0.91 (P<0.01). The assessment criteria used in this study are expected to provide a useful basis designing a temperament test horses.

Breeding of Hansaengjam a Parental Sex-limited Larval Marking Strain Suitable for Spring Rearing Season

  • Kang, Pil-Don;Sung, Gyoo-Byung;Kim, Kee-Young;Lee, Hee-Sam;Kim, Mi-Ja;Ji, Sang-Duk;Hong, In-Pyo;Kweon, Hae-Yong;Park, Kwang-Young;Lee, Kwang-Gil;Sohn, Bong-Hee
    • International Journal of Industrial Entomology and Biomaterials
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    • v.23 no.1
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    • pp.179-182
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    • 2011
  • Normally the first generation of hybrid is supplied for farm rearing, precise and labor saving sex discrimination is needed. The new strain "Hansaengjam" is sex-limited (female:larval markings, male:no-markings) strain which was bred from both sex-limited strain Japanese originated Jam 153 and Chinese originated strain Jam 154. Productivity test of Hansaengjam during 2007 and 2009 showed high healthiness and cocoon yield. The Hansaengjam is evaluated as an excellent strain from healthiness, cocoon yield and other test results.

Genotoxic Evaluation of Gryllus Bimaculatus in 3 Sets of Mutagenesis Test

  • Ahn, Mi-Young;Bae, Hye-Jin;Lee, Byung-Mu;Ryu, Kang-Sun;Kim, Iksoo;Kim, Jin-Won
    • Proceedings of the Korean Society of Toxicology Conference
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    • 2003.10b
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    • pp.121-121
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    • 2003
  • Cricket (Gryllus bimacutus) is mass-bred in 6 cycles per one year in insect farms. They are used as dry or live foods for animals, tropical fish, reptile and amphibians. Therefore, it is necessary to study the genotoxicity of whole bodies of G. bimaculatus.(omitted)

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Mechanical Test Methods for Compost Maturity

  • Kwon, Soon-Ik;Kim, Min-Kyeong;Jung, Goo-Bok;Park, Woo-Kyun;Hong, Seung-Gil;Kim, Kwon-Rae
    • Korean Journal of Soil Science and Fertilizer
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    • v.44 no.5
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    • pp.959-961
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    • 2011
  • Manure-based composts can have detrimental effects on the agricultural lands and crops if they are applied without proper stabilization process. Composting is well-known method for the stabilization of manure-based composts and the extent of composting could be examined by maturity test. Among various methods to examine compost maturity, the performances of two mechanical methods (Solvita and CoMMe-100) were examined and the test results were compared with seed germination test. The mechanical methods are considered to be simple and relatively objective. Also they are cost- and time-efficient. Ten commercially available composts collected in Korea were used for this study. Basically, the maturity determined with these mechanical methods was in good agreement with the seed germination test. However, it appeared that the index levels of compost maturity indicated more maturity by CoMMe-100 than Solvita for the same compost. The differences between maturity index levels determined by both methods were able to be minimized through extending the reaction time for CoMMe-100 and adjusting index level for maturity determination in the standard color chart.

Evaluation of the Cardiorespiratory Endurance of Jeju Crossbred Horses according to Exercise Intensity (제주산마의 운동강도별 심폐지구력 측정)

  • Kim, Nam-Young;Park, Yong-Sang;Ko, Moon-Suck;Chae, Hyun-Seok;Cho, Won-Mo;Cho, In-Chul;Hong, Hyun-Ju;Park, Ji-Yun;Cho, Sang-Rae;Kang, Tae-Young
    • Journal of Veterinary Clinics
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    • v.30 no.6
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    • pp.449-452
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    • 2013
  • The present study was conducted to measure the cardiorespiratory endurance of Jeju crossbred horses according to hill tests and exercise intensity, thereby providing foundational data for evaluation of running capability of horses. The nine Jeju crossbred horses were subjected to the hill test with an inclination of around $30^{\circ}$ and six out of nine horses were used to measure the cardiorespiratory endurance according to three different exercise intensities (moderate, heavy, and very heavy). The running time for the hill test was 205 sec on average, while high deviation was observed between horses from 167 to 258 sec. In addition, the running time for the hill test was highly correlated with moderate exercise. While no significant difference in average heart rate was found between exercise intensities, a trend of large variation was found in the average heart rate between horses. The heart rate recovery was highly correlated with heavy exercise and very heavy exercise. The results showed that no significant difference in average heart rate was found among three exercise intensities, but there are large individual variations among horses.

A Study on Testing the Korean Cataloguing Rules through Analyzing the RDA Test (RDA 테스트 분석을 통해 본 한국목록규칙의 테스트 방안에 관한 연구)

  • Lee, Mihwa;Hyun, Moonsoo
    • Journal of Korean Library and Information Science Society
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    • v.46 no.1
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    • pp.155-176
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    • 2015
  • This study was for suggesting the test methods in the revision process of the cataloging rules to understand the problem of draft cataloging rules and to apply the new cataloging rules correctly in libraries instead of collecting the opinions by the traditional seminar and conference in the process of revising KCR, KCR2, KCR3, KCR4. For this study, the literature review and the case study were used as the research methods. The case study was based on the US RDA Test by US RDA Test Coordinating Committee. The evaluation areas of the test were cataloging rules, record creation and system development by reflecting the new cataloging rules, user, and cost. The data for the analysis was the creation of bibliographic records and authority records by librarians, and the question investigations that were the use of institutions, librarians, and users. This study would contribute to revise the cataloging rules in future by analyzing the errors of applying new rules to bibliographic record and by investigating the difficulties of applying rules in completing the bibliographic record. Also, the libraries could be easy to decide to implement the new rules from the creation time of bibliographic record by new rules and the learning curve of new rules.