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http://dx.doi.org/10.5307/JBE.2011.36.2.140

Rapid Detection Kit for Salmonella typhimurium  

Kim, Gi-Young (National Academy of Agricultural Science, RDA)
Yang, Gil-Mo (National Academy of Agricultural Science, RDA)
Park, Saet-Byeol (National Academy of Agricultural Science, RDA)
Kim, Yung-Hwun (National Academy of Agricultural Science, RDA)
Lee, Kang-Jin (National Academy of Agricultural Science, RDA)
Son, Jae-Yong (National Academy of Agricultural Science, RDA)
Kim, Hyuck-Joo (National Academy of Agricultural Science, RDA)
Lee, Sae-Rom (National Academy of Agricultural Science, RDA)
Publication Information
Journal of Biosystems Engineering / v.36, no.2, 2011 , pp. 140-146 More about this Journal
Abstract
This study was performed to develop a rapid test kit for pathogenic Salmonella in various samples. The rapid detection kit has been fabricated based on nitrocellulose lateral-flow strip. Colloidal gold and biotin conjugated Salmonella antibodies were used as a tag and a receptor, respectively. Manually spotted Salmonella antibody and Neutravidin on nitrocellulose membrane were used as test and control lines, respectively. Feasibility of the rapid kit to detect Salmonella typhimurium in samples were evaluated. The intensity of the color of the test line started to increase with the samples in which higher concentration of the cells were contained. The sensitivity of the sensor was $10^6$ cfu/mL Salmonella spiked in PBS. Also, the rapid test kit could detect $10^6$ cfu/mL of Salmonella in chicken meat extract.
Keywords
Rapid test kit; Salmonella; Lateral flow;
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Times Cited By KSCI : 3  (Citation Analysis)
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