• 한국식품과학회지
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• 제42권5호
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• pp.593-597
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• 2010

본 연구에서는 현미의 미강으로부터 생리활성이 있는 페놀산물질을 얻기 위해 다양한 추출법을 시도하였고, 얻어진 미강 페놀산 농축물의 성분 분석 및 항산화 활성에 대해 조사하였다. 미강 추출물 중 알칼리 가수분해를 시행한 다음 에틸아세테이트로 분획하여 얻은 RBE-II의 총 폴리페놀 함량과 항산화 활성이 가장 높은 것으로 조사되었고, 페놀산 성분의 농축 및 정제를 위해 Sep-pak $C_{18}$ Vac cartridge를 통과시켜 미강 페놀산 농축물(F1-RBE-II)을 얻었다. HPLC 분석을 통해 얻어진 페놀산 성분 중 ferulic acid가 가장 많았고, p-coumaric acid, sinapic acid, vanillic acid, 그리고 syringic acid가 존재하였으며, 그 구성성분이 주로 hydroxycinnamic acid 계열에 속한다는 것을 알 수 있었다. DPPH radical과 ABTS radical 소거능에서 미강 페놀산 농축물(F1-RBEII)는 강력한 항산화 활성을 나타냈으며, ${\beta}$-CLMS을 이용한 지질산화 유도반응에서도 우수한 peroxy radical 저해능을 발휘하여, 합성 항산화제인 BHA의 활성과 비교했을 때 높은 과산화 지질억제력을 보였다. 본 연구를 통해 미강 페놀산 농축물의 성분 및 강한 항산화 능력이 확인됨으로천연 항산화제 및 지방 산화 방지제로 개발하기에 부족함이 없는 것으로 사료된다.

• Radiation Oncology Journal
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• 제5권2호
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• pp.75-82
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• 1987

원자력병원 싸이클로트론으로 발생되는 고에너지 속중성자선의 생물학적효과에 대한 연구의 일환으로 RBE를 측정하였다. 대상은 Walker 256 carcinosacoma 및 정상조직으로 마우스 위장관 및 골수를 이용하였고 단일 및 분할조사에 대한 반응을 관찰하였다. 마우스 공장소낭선세포의 단일전신조사에 의한 RBE는 2.8이었다. 단일조사시 이식암 Walker 250의 TCD 50에 대한 RBE, 마우스 골수 및 위장관의 RBE는 각각 1.9, 1.9, 1.5이었다. 분할조사시 Walker 256의 RBE는 분할횟수의 증가에 따라 감소하였고 1회 분할조사량의 증가에 따라 증가하였다.

• Journal of Radiation Protection and Research
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• 제12권2호
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• pp.1-8
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• 1987

원자력병원(原子力病院) 싸이클로트론 중성자선(中性子線)의 방사선생물학적(放射線生物學的) 특성파악을 위하여 생쥐를 이용하여 RBE를 측정하였다. 단일전신조사(單一全身照射)에 의한 생쥐공장소양선세포(空腸小襄腺細胞)의 RBE는 2.8이었다. 이 RBE치(値)는 상이(相異)한 조직(組織)이나 기관(器管)종류에 있어서 분할(分割), 조사방법(照射方法)의 변화에 따라 상이(相異)하게 변하였다. 위장관(胃腸管) 및 골수(骨髓)의 단일조사(單一照射)에 대한 RBE는 각각 1.5 및 1.9였고, 분할조사시(分割照射時) 1회, 조사량(照射量), 총조사량(總照射量) 및 분할(分割)횟수 등에 따른 RBE의 변화를 관찰하였다.

• 한국의학물리학회지:의학물리
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• 제28권1호
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• pp.11-15
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• 2017

Relative biological effectiveness (RBE) of particle beam needs to be evaluated at particle beam therapy centers before the clinical application of the particle beam. However, since RBE analysis is implemented manually, it is useful to have a tool that can easily and effectively handle the data of experiments to generate cell survival curve and to analyze RBE simultaneously. In this work, the development of a program for RBE analysis of particle beam therapy was presented. This RBE analysis program was developed to include two parts; fitting the cell survival curves to linear-quadratic model and calculating the RBE values at a certain endpoint using fitting results. This program was also developed to simultaneously compare and analyze the template results that stored experiment data with photon and particle beam irradiations. The results of the cell survival curve obtained by each irradiation can be analyzed by the user on a desired data after reading the template stored in the easy-to-use excel file. The analysis results include the cell survival curves with error range, which are appeared in the screen and the ${\alpha}$ and ${\beta}$ parameters of linear-quadratic model with 95% confidence intervals, RBE values, and $R^2$ values to evaluate goodness-of-fit of survival curves to model, which are stored in a text cvs file. This software can generate cell survival curve, fit to model, and calculate RBE all at once with raw experiment data, so it helps users to save time for data handling and to reduce the possibility of making error on analysis. As a coming plan, we will create a user-friendly graphical user interface to present the results more intuitively.

• 한국의학물리학회지:의학물리
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• 제31권1호
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• pp.1-7
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• 2020

Hadron therapy, such as carbon and helium ions, is increasingly coming to the fore for the treatment of cancers. Such hadron therapy has several advantages over conventional radiotherapy using photons and electrons physically and clinically. These advantages are due to the different physical and biological characteristics of heavy ions including high linear energy transfer and Bragg peak, which lead to the reduced exit dose, lower normal tissue complication probability and the increased relative biological effectiveness (RBE). Despite the promising prospects on the carbon ion radiation therapy, it is in dispute with which bio-mathematical models to calculate the carbon ion RBE. The two most widely used models are local effect model and microdosimetric kinetic model, which are actively utilized in Europe and Japan respectively. Such selection on the RBE model is a crucial issue in that the dose prescription for planning differs according to the models. In this study, we aim to (i) introduce the concept of RBE, (ii) clarify the determinants of RBE, and (iii) compare the existing RBE models for carbon ion therapy.

• Journal of Microbiology and Biotechnology
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• 제27권10호
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• pp.1885-1891
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• 2017

In this study, we evaluated the inhibitory effect of a rice bran mixture extract (RBE) on Brucella abortus pathogenesis in professional (RAW 264.7) and nonprofessional (HeLa) phagocytes. We fermented the rice bran mixture and then extracted it with 50% ethanol followed by gas chromatography-mass spectrometry to identify the components in RBE. Our results clearly showed that RBE caused a significant reduction in the adherence of B. abortus in both cell lines. Furthermore, analysis of phagocytic signaling proteins by western blot assay revealed that RBE pretreatment resulted in inhibition of phosphorylation of JNK, ERK, and p38, leading to decline of internalization compared with the controls. Additionally, the intensity of F-actin observed by fluorescence microscopy and FACS was remarkably reduced in RBE-pretreated cells compared with control cells. However, the intracellular replication of B. abortus within phagocytes was not affected by RBE. Taken together, these findings suggest that the phagocytic receptor blocking and suppressive effects of RBE on the MAPK-linked phagocytic signaling pathway could negatively affect the invasion of B. abortus into phagocytes.

• 생약학회지
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• 제46권2호
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• pp.140-147
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• 2015

The antioxidative activities and anti-inflammatory effects of rice bran and wheat bran were investigated. To determine the antioxidant activity, total phenolic content. DPPH reaction, ABTS reaction and FRAP assay were tested. To investigate the anti-inflammatory effect, the inhibition of NO production, IL-6 production and TNF-${\alpha}$ production were observed. The total phenolic contents were 46.4 mg/g in the BuOH fraction of rice bran (RBB) and 69.0 mg/g in BuOH fraction of wheat bran (WBB). EtOAc fraction of rice bran (RBE), EtOAc fraction of wheat bran (WBE), RBB and WBB showed high radical scavenging activity at a concentration of 5 mg/ml. Scavenging activity of DPPH radical were 89.04% in RBB and 91.53% in WBE. Scavenging activity of ABTS radical were 88.53% in RBB and 90.39% in WBB. In case of FRAP assay, RBE reduced 0.805 mM/mg of Fe²⁺ and WBE reduced 1.521 mM/mg of Fe²⁺. RBB, RBE, WBB and WBE showed concentration dependent inhibition of LPS induced NO production (RBE: 57.38%, WBE: 76.85%), IL-6 production (RBE: 92.08%, WBB: 92.57%), TNF-α production (RBE: 86.33%, WBE: 85.05%) at a concentration of 100 μg/ml of each fraction. These results showed that RBB, RBE, WBB and WBE have strong antioxidative activities and anti-inflammatory effects and show the possibilities of a new natural antioxidants and anti-inflammatory medicines.

• Journal of Radiation Protection and Research
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• 제23권1호
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• pp.17-23
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• 1998

자주달개비 4430 클론에 있어서 $^{252}Cf$ 중성자에 의해 유발되는 유전자 돌연변이의 선량반응 관계를 확립함과 동시에 TSH 분홍돌연변이를 유발함에 있어서 X-선에 대비한 중성자의 생물학적 효과비를 산출하였다. 돌연변이의 분석은 공시재료의 방사선조사 후 4주 이상 이뤄졌으며 최대변이율을 보이는 조사후 6일부터 13일까지의 분석결과로부터 돌연변이빈도를 산출하였다 가용한 실험범위 내에서 중성자 선량의 증가에 따라 돌연변이빈도의 선형적 증가 양상을 나타내었다. X-선에 비교하여 산출한 중성자의 RBE값은 3.1에서 6.8의 범위를 보였으며 이러한 값은 일반적으로 인지되고 있는 중성자의 RBE값에 비해 상당히 낮은 값이다.

• 생약학회지
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• 제45권4호
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• pp.326-331
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• 2014

In this study, we investigated pharmacologic activity of rice bran ethyl acetate fraction (RBE), based on their osteoblast enhancing effects. It has been found that REB have a stimulatory effect on the commitment of bi-potential mesenchymal precursor C2C12 cells into osteoblasts in the presence of BMP-2. Furthermore, RBE enhanced the BMP-2-stimulated induction of ALP, an early phase biomarker of osteoblast differentiation. In addition, Western blot analysis showed RBE enhanced the BMP-2-stimulated phosphorylation of p38, but not those of ERK or JNK. These findings show RBE has the potential to enhance the BMP-2-mediated commitment of C2C12 cells into osteoblasts and their differentiation through p38 activation.

• 약학회지
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• 제55권6호
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• pp.456-461
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• 2011

The aim of the present study is to investigate the anti-inflammatory effect of a Rice Bran Ethanol Extract (RBE). Inflammation, such as a bacterial infection in vivo metabolites, such as external stimuli or internal stimuli to the defense mechanisms of the biological tissue a variety of intracellular regulatory factors deulin inflammatory TNF-${\alpha}$, IL-$1{\beta}$, IL-6, IL-8, such as proinflammatory cytokines, prostagrandin, lysosomal enzyme, free radicals are involved in a variety of mediators. The present study was designed to determine the effect of the RBE on pro-inflammatory factors such as NO, iNOS expression and TNF-${\alpha}$, IL-$1{\beta}$, IL-6 in lipopolysaccharide (LPS) - stimulated RAW264.7 macrophages cells. The cell toxicity was determined by MTS assay. To evaluate of anti-inflammatory effect of RBE, amount of NO was measured using the NO detection kit and the iNOS expression was measured by reverse transcriptase polymerase chain reaction (RT-PCR). And proinflammatory cytokines were measured by ELISA kit. As a result, the RBE reduced NO, iNOS expression and TNF-${\alpha}$, IL-$1{\beta}$, IL-6 production without cytotoxicity. Our results suggest that the RBE may have an anti-inflammatory property through suppressing inflammatory mediator productions and appears to be useful as an anti-inflammatory material.