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Properties and Antioxidative Activities of Phenolic Acid Concentrates of Rice Bran  

Jung, Eun-Hee (Korea Food Research Institute)
Hwang, In-Kyeong (Department of Food and Nutrition, Seoul National University)
Ha, Tae-Youl (Korea Food Research Institute)
Publication Information
Korean Journal of Food Science and Technology / v.42, no.5, 2010 , pp. 593-597 More about this Journal
Abstract
This study investigated the properties and antioxidative activities of phenolic acid concentrates of rice bran. Rice bran contains bioactive substances such as phenolic compounds, which can provide health benefits as natural antioxidants. This study examined how levels of phenolic acids can be obtained efficiently through various extraction methods. The extractions of defatted rice bran were followed by using ethylacetate (RBE-I), ethylacetate after alkaline hydrolysis (RBE-II), and 80% methanol (RBE-III). For all extracts, yields (%), total polyphenol contents (TPC), various phenolic acids and antioxidative activities were estimated. RBE-II had the highest total polyphenol contents (526.72 mg/100 g rice bran) and showed high antioxidative activity (74.7%). To concentrate the phenolic acids, RBE-II was passed through Sep-pak $C_{18}$ Vac cartridge and F1-RBE-II was collected by the elution of 50% methanol. The total phenolic content of F1-RBE-II (736.8 mg/100 g rice bran) was higher than that of RBE-II (367.1 mg/100 g rice bran), and the ratios of ferulic acid (73%) and sinapic acid (14%) increased. As RBE-II was analysed by HPLC, 6 different phenolic acids were found via chromatography, whereas F1-RBE-II showed 5 different peaks and the major phenolic acid was identified as ferulic acid. The ABTS radical scavenging activity of F1-RBE-II was the highest among the rice bran extracts. In a ${\beta}$-carotene-linoleic acid model system, linoleic acid oxidation was reduced by F1-RBE-II (73%) and RBE-II (35%).
Keywords
rice bran; phenolic acid; antioxidative activity; ferulic acid;
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1 Re R, Pellegrini N, Proteggente A, Pannala A, Yang M, Rice- Evans C. Antioxidant activity applying an improved ABTS radical cation decolorization assay. Free Radical Bio. Med. 26: 1231-1237 (1999)   DOI   ScienceOn
2 Hiroe K, Masashi H, Kanae H, Kayo A, Hisaji T. Antioxidant properties of ferulic acid and its related compounds. J. Agr. Food Chem. 50: 2161-2168 (2002)   DOI   ScienceOn
3 Kaur C, Kapoor HC. Anti-oxidant activity and total phenolic content of some Asian vegetables. Int. J. Food Sci. Tech. 37: 153-161 (2002)   DOI   ScienceOn
4 Kim SR, Ahn JY, Lee HY, Ha TY. Various properties and phenolic acid contents of rices and rice brans with different milling fractions. Korean J. Food Sci. Technol. 36: 930-936 (2004)   과학기술학회마을
5 Kim YS. Functionality of fermented rice bran and its utilization. Ministry of Agriculture, Seoul, Korea. pp. 31-32 (2003)
6 Kafui KA, Rui HL. Antioxidant activity of grains. J. Agr. Food Chem. 50: 6182-6187 (2002)   DOI   ScienceOn
7 Singleton VL, Rossi JA. Colorimetry of total phenolics with phosphomolybdic-phosphotungstic acid reagents. Am. J. Enol. Viticult. 16: 144-158 (1965)
8 Fausta N, Mirella N, Maurizio DF, Cristina S. Benzoic and cinnamic acid derivatives as antioxidants: Structure-activity relation. J. Agr. Food Chem. 47: 1453-1459 (1999)   DOI   ScienceOn
9 Blois MS. Antioxidant determination by the use of stable free radical. Nature 181: 1199-1201 (1958)   DOI   ScienceOn
10 Taga MS, Miller EE, Pratt DE. Chia seeds as a source of natural lipid antioxidants. J. Am. Oil Chem. Soc. 61: 928-931 (1984)   DOI
11 Bourne LC, Rice-Evans C. Bioavailability of ferulic acid. Biochem. Bioph. Res. Co. 253: 222-227 (1998)   DOI   ScienceOn
12 Chesson A, Provan GJ, Russell WR, Scobbie L, Richardson AJ, Stewart C. Hydroxycinnamic acids in the digestive tract of livestock and human. J. Sci. Food Agr. 79: 373-378 (1999)   DOI   ScienceOn
13 Sosulski F, Krygier K, Hogge L. Free, esterified, and insolublebound phenolic acids. 3. Composition of phenolic acids in cereal and potato flours. J. Agr. Food Chem. 30: 337-340 (1982)   DOI
14 Graf E, Eaton JW. Antioxidant function of phytic acid. Free Radical Bio. Med. 8: 61-69 (1990)   DOI   ScienceOn
15 Zhou Z, Robards K, Helliwell S, Blanchard C. The distribution of phenolic acids in rice. Food Chem. 47: 401-406 (2004)
16 Simin L, Meir JS, Frank BH, Edward G, Eric R, Joann EM, Charles HH, Walter CW. Whole-grain consumption and risk of coronary heart disease: Result from the nurse's health study. Am. J. Clin. Nutr. 70: 412-419 (1999)
17 Scalbert A, Manach C, Morand C, Remesy C. Dietary polyphenols and the prevention of diseases. Crit. Rev. Food Sci. 45: 287-306 (2005)   DOI   ScienceOn
18 Cheruvanky R. Phytochemical Functional Foods, Phytochemical Products: Rice Bran. CRC Press, Boca raton, FL, USA. pp. 347-376 (2000)
19 Chun HS, You JE, Kim IH, Cho JS. Comparative antimutagenic and antioxidative activities of rice with different milling fractions. Korean J. Food Sci. Technol. 31: 1371-1377 (1999)
20 Ogiwara T, Satoh K, Murakami Y, Unten S, Atsu T, Sakagami H, Fujisawa S. Radical scavenging activity and cytotoxicity of ferulic acid. Anticancer Res. 22: 2711-2717 (2002)
21 Faulds CB, Williamson G. The role of hydroxycinnamates in the plant cell wall, review. J. Sci. Food Agr. 79: 393-395 (1999)   DOI   ScienceOn
22 Kushi LH, Meyer KA, Jacobs JD. Grains, legumes, and chronic disease risk reduction. Evidence epidemiological studies. Am. J. Clin. Nutr. 70: 451s-458s (1999)