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Effects of Osteoblast Differentiation via C2C12 Cell by Rice Bran Ethyl acetate Fraction  

Moon, Jungsun (College of Pharmacy, SahmYook University)
Moon, Seung Hee (Laboratory of Translational Therapeutics, Pharmacology Research Center, Division of Drug Discovery Research, Korea Research Institute of Chemical Technology)
Choi, Sungsook (College of Pharmacy, SahmYook University)
Lee, Sookyeon (Korea Re-Creation Institute of Phytochemicals, College of Pharmacy, SahmYook University)
Yim, Dongsool (College of Pharmacy, SahmYook University)
Publication Information
Korean Journal of Pharmacognosy / v.45, no.4, 2014 , pp. 326-331 More about this Journal
Abstract
In this study, we investigated pharmacologic activity of rice bran ethyl acetate fraction (RBE), based on their osteoblast enhancing effects. It has been found that REB have a stimulatory effect on the commitment of bi-potential mesenchymal precursor C2C12 cells into osteoblasts in the presence of BMP-2. Furthermore, RBE enhanced the BMP-2-stimulated induction of ALP, an early phase biomarker of osteoblast differentiation. In addition, Western blot analysis showed RBE enhanced the BMP-2-stimulated phosphorylation of p38, but not those of ERK or JNK. These findings show RBE has the potential to enhance the BMP-2-mediated commitment of C2C12 cells into osteoblasts and their differentiation through p38 activation.
Keywords
Rice bran ethyl acetate fraction (RBE); BMP-2; Osteoblast Differntiation; p38;
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