• Title/Summary/Keyword: RAP

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Crystal structure of unphosphorylated Spo0F from Paenisporosarcina sp. TG-14, a psychrophilic bacterium isolated from an Antarctic glacier

  • Lee, Chang Woo;Park, Sun-Ha;Jeong, Chang Sook;Lee, Chang Sup;Hong, Jong Wook;Park, Hyun Ho;Park, Hyun;Park, HaJeung;Lee, Jun Hyuck
    • Biodesign
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    • v.6 no.4
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    • pp.84-91
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    • 2018
  • Spo0F is a response regulator that modulates sporulation, undergoes phosphorylation for phosphorelay signal transduction, and interacts with various regulatory proteins; however, the mechanisms through which phosphorylation induces structural changes and regulates interactions with binding partners remain unclear. Here, we determined the unphosphorylated crystal structure of Spo0F from the psychrophilic bacterium Paenisporosarcina sp. TG-14 (PaSpo0F) and established a phosphorylation-state structural model. We found that PaSpo0F underwent structural changes (Lys54 and Lys102) by phosphorylation and generated new interactions (Lys102/Gln10 and Lys54/Glu84) to stabilize the ${\beta}4/{\alpha}4$ and ${\beta}1/{\alpha}1$ loop structures, which are important target-protein binding sites. Analysis of Bacillus subtilis Spo0 variants revealed movement by BsSpo0F Thr82 and Tyr84 residues following interaction with BsSpo0B, providing insight into the movement of corresponding residues in PaSpo0F (Thr80 and Tyr82), with further analysis of BsSpo0F/BsRapH interaction revealing alterations in the ${\beta}4/{\alpha}4$ loop region. These results suggest that phosphorylation-induced structural rearrangement might be essential for PaSpo0F activation and expand the understanding of Spo0F-specific activation mechanisms during sporulation.

A Study on Characteristics of Underground Rappers' Fashion - Focusing on the Comparison Between China and Korea - (언더그라운드 래퍼 패션의 특성 - 한국과 중국의 비교를 중심으로 -)

  • Fan, Keyu;Ha, Jisoo
    • Fashion & Textile Research Journal
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    • v.24 no.5
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    • pp.493-504
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    • 2022
  • This study aims to gain a deeper understanding of the fashion styles of underground rappers in China and South Korea. Due to rappers' fashion influence on modern fashion trends, research on rapper fashion has been conducted steadily in the field of apparel. Qualitative research methodologies including literature research and in-depth interviews were the primary techniques used to solve the research questions. In-depth interviews were conducted with 10 Chinese underground rappers and 10 Korean underground rappers to reveal and explore their fashion style and view of fashion. As a result, the participants' fashion styles were categorized into four styles: authentic hip-hop fashion style, popularized hip-hop fashion style, easy sports casual style, and trendy street style. Rappers from both countries consider that their identity as rappers can be demonstrated through their hip-hop fashion style. The influence of hip-hop culture and the popularity of rap music differs between China and Korea. It affects not only the rapper's musical characteristics, but also their fashion style. While Korean underground rappers' fashions style is trendier, Chinese underground rappers' fashions style displays stronger characteristics of hip-hop fashion. Due to the public's negative view of rappers, some Korean underground rappers intentionally try to hide their identity by wearing a fashion style that differs from the authentic rapper image. Understanding the fashion styles of underground rappers in Korea and China is expected to assist in predicting future hip-hop culture and rapper fashion trends.

Effect of Pediatric Integrative Manual Therapy, a Novel Mobilization with Facilitation Movement Technique, on Congenital Muscular Torticollis after Cervical Rotation and Head angle: A Case Report (선천성 근성 사경에 대한 새로운 촉진 기법을 이용한 소아 통합 도수치료적용 후 경추각도의 변화와 머리각도 변화: 단일사례연구)

  • Seung-hyoek Song;Gue-jung Hwang;Tae-gyu Seo;Jae-deung Kim;Won-jeong Whang
    • The Journal of Korean Academy of Orthopedic Manual Physical Therapy
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    • v.29 no.2
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    • pp.77-91
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    • 2023
  • Background: Congenital muscular torticollis results in reduced head mobility, such as cervical rotation, due to the abnormal size and contraction of the sternocleidomastoid muscle. Korea Pediatric integrative manual therapy and stretching are recommended to improve head rotation upper cervical spine mobility. Therefore, in this study, the effect of the new PIMT was investigated. Methods: The patient is a 3.5 month-old diagnosed with congenital muscular torticollis (CMT). Due to the limitation of head rotation and cervical spine rotation and flexion mobility, the child visited a rehabilitation center and after diagnosis, Pediatric integrative manual therapy (PIMT) treatment was performed five times a week for a total of 15 weeks. The child's head rotation and flexion limitation and plagiocephaly were evaluated. Results: In conclusion, this study shows that compared to other treatments, PIMT approach is a more effective treatment for improving head rotation and cervical limitation for range of motion in CMT infants. Conclusion: PIMT approach was effective in improving cervical rotation and Head lateral flexion mobility and plagiocephaly in CMT patients.

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소규모 고도 수처리 기술 -가정용 정수시스템 중심으로-

  • Sung, Kyung-Hak;Cho, Bong-Yeon;Park, Ho-Wan
    • Proceedings of the Membrane Society of Korea Conference
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    • 1997.06a
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    • pp.207-231
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    • 1997
  • The use household water purifier is continuously increasing due to the distrust of the piped, rap water, which may be caused by the deterioration of water quality and water supply facilities in Korea. The water purifier distributing in the domestic market is the membrane type that composes of membrane as a basic filter, sediment filter as a prefilter and activated carbon filter instead of one purifying water system. The membrane type using as a water cleaning system is mainly the method of micro filtration(NIF) as well as ultra filtration(UF) and reverse osmosis(RO). The types of MF and UF are using to remove suspended solids and virus, and RO is using to remove ionic compound in chemical organic compounds. At the beginning the household water purifier was imported as end-product or assembled by only importing module from small businesses in Korea. Therefore, people was hard to confide in the product quality because the sales policy and management of water purifier by small business were not effectively organized. However, the defects of water purifier having up to now ate improving by the efforts of special branches of water purifier and large enterprises, and new great products are developing and producing. To develop the best quality product of water purifier in Korea, it should be considered seasonal water characteristrics rather than any other condition. For example, the water purifier can be affected by the water temperature change, increase of suspended solids in the rainy season and inflow of raw water having concentration in a dry season. The subject we have to investigate in the future is to develop the water purifying system adaptable in Korea which can treat the water quality using close analyses of local and seasonal water characteristics.

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Analysis of common and characteristic actions of Panax ginseng and Panax notoginseng in wound healing based on network pharmacology and meta-analysis

  • Zhen Wang ;Xueheng Xie ;Mengchen Wang ;Meng Ding ;Shengliang Gu ;Xiaoyan Xing;Xiaobo Sun
    • Journal of Ginseng Research
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    • v.47 no.4
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    • pp.493-505
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    • 2023
  • In recent years, an increasing number of reports have explored the wound healing mechanism of these two traditional Chinese herbal medicines- Panax ginseng and Panax notoginseng, but there is no systematic research on the related core functions and different mechanisms in the treatment of wound healing up to now. Based on network pharmacology and meta-analysis, the present work aimed to comprehensively review the commonality and diversity of P. ginseng and P. notoginseng in wound healing. In this study, a wound healing-related "ingredients-targets" network of two herbs was constructed. Thereafter, meta-analysis of the multiple target lists by Metascape showed that these two medicines significantly regulated blood vessel development, responses to cytokines and growth factors and oxygen levels, cell death, cell proliferation and differentiation, and cell adhesion. To better understand the discrepancy between these two herbs, it was found that common signaling pathways including Rap1, PI3K/AKT, MAPK, HIF-1 and Focal adhesion regulated the functions listed above. In parallel, the different pathways including renin-angiotensin system, RNA transport and circadian rhythm, autophagy, and the different metabolic pathways may also explained the discrepancies in the regulation of the above-mentioned functions, consistent with the Traditional Chinese Medicine theory about the effects of P. ginseng and P. notoginseng.

Optical, thermal and gamma ray attenuation characteristics of tungsten oxide modified: B2O3-SrCO3-TeO2-ZnO glass series

  • Hammam Abdurabu Thabit;Abd Khamim Ismail;M.I. Sayyed;S. Hashim;I. Abdullahi;Mohamed Elsafi;K. Keshavamurthy;G. Jagannath
    • Nuclear Engineering and Technology
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    • v.56 no.1
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    • pp.247-256
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    • 2024
  • The glass series modified by tungsten oxide was created using the compounds (75-x) B2O3- 10SrCO3- 8TeO2- 7ZnO - xWO3, where x = 0, 1, 5, 10, 22, 27, 34, and 40% mole percentage. A UV-visible spectrophotometer and thermogravimetric-differential thermal analysis (TG-DTA) methods were employed to characterize the specimen's optical and phase transition attributes, respectively. The mass-attenuation coefficient (AC) of all created glasses from BSTZW0 to BSTZ7 was estimated using Geant4 code from 0.05 to 3 MeV and compared to the XCOM software results, with a relative difference of less than 2% between the two results. The increase of WO3 percentage lead to an increase in the Linear-AC at each studied energy, and this is mainly due to the fact that the higher the percentage of WO3 in the glass increases its density which causes an increase in the Linear-AC, so an energy of 0.06 MeV, as an example, the values of the Linear-AC was 4.009, 4.509, 5.442, 6812, 8.564, 9.856, 10.999 and 11.628 cm-1 form BSTZW0 too BSTZW7, respectively. The Half-VL (value layer), Mean-FP (free path), Tenth-VL, and Radiation attenuation performance (RAP) were also calculated for the current BSTZW-glass samples and revealed that BSTZW7 had the best gamma ray attenuation performance at all discussed energies when compared to other studied glass samples.

Deterioration Assessment for Conservation Sciences of the Five Storied Stone Pagoda in the Jeongrimsaji Temple Site, Buyeo, Korea (부여 정림사지 오층석탑의 보존과학적 풍화훼손도 평가)

  • Kim, Yeong-Taek;Lee, Chan-Hee;Lee, Myeong-Seong
    • Economic and Environmental Geology
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    • v.38 no.6 s.175
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    • pp.675-687
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    • 2005
  • The rocks of the five storied stone pagoda in the Jeongrimsaji temple site are 149 materials in total with porphyritic biotite granodiorite. They include pegmatite veinlet, basic xenolith and evenly developed plagioclase porphyry. This stone pagoda has comparably small fracture and cracks which are farmed in the times of rock properties, but surface exfoliation and granular decomposition are in process actively since the rocks are generally weakened from the influence of air contaminants and acid rain. Structural instability of constituting rocks in the 4th roof materials are observed to occur from distortion and tilt. Such instability is judged to threat stability of the upper part of the stone pagoda. Also, chemical weathering is operating even more as the contaminants, ferro-manganese hydroxides eluted from water-rock interaction on the rock surface. Most of the rock surface is covered with yellowish brown, dark black and light gray contaminants, and especially occur in the lower part of the roof rocks on each floor. The roof underpinning rocks are severe in surface pigmentation from manganese hydroxides and light gray contaminants. The surface of rocks lives bacteria. algae, lichen, or moss and diverse productions in colors of light gray, dark Bray and dark green. Grayish white crustose lichen grows thick on the surface with darkly discolored by fungi and algae in the first stage on basement rocks, and weeds grows wild on the upper part of each roof rocks. This stone pagoda must closely observe the movements of the upper part rock materials through minute safety diagnosis and long term monitoring for structural stability. Especially since the surface discoloration of rocks and pigmentation of secondary contaminants are severe, establishment of general restoration and scientific conservation treatment are necessary through more detailed study for this stone pagoda.

A Study on An Integrated GEO/TES with Geothermal Heat Exchanger and Thermal Ice Storage (지중열 교환기와 빙축열조(Thermal Ice Storage)를 연계시킨 통합 지중열-빙축열조 시스템(Integrated GEO/TES))

  • Lohrenz ED.;Hahn Jeongsang;Han Hyuk Sang;Hahn Chan;Kim Hyoung Soo
    • Economic and Environmental Geology
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    • v.38 no.6 s.175
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    • pp.717-729
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    • 2005
  • Peak cooling load of large buildings is generally greater than their peak heating load. Internal and solar heat gains are used fur selection of adquate equipment in large building in cold winter climate like Canada and even Korea. The cost of geothermal heat exchanger to meet the cooling loads can increase the initial cost of ground source heat pump system to the extend less costly conventional system often chosen. Thermal ice storage system has been used for many years in Korea to reduce chiller capacity and shift Peak electrical time and demand. A distribution system designed to take advantage of heat extracted from the ice, and use of geothermal loop (geothermal heat exchanger) to heat as an alternate heat source and sink is well known to provide many benifits. The use of thermal energy storage (TES) reduces the heat pump capacity and peak cooling load needed in large building by as much as 40 to $60\%$ with less mechanical equipment and less space for mechanical room. Additionally TES can reduce the size and cost of the geothermal loop by 1/3 to 1/4 compared to ground coupled heat pump system that is designed to meet the peak heating and cooling load and also can eliminate difficuties of geothermal loop installation such as space requirements and thermal conditions of soil and rock at the urban area.

Analysis on the volume variation of bag-net in set-net by acoustic telemetry (음향 텔레메트리에 의한 정치망 원통의 체적 변화 해석)

  • Tae, Jong-Wan;Shin, Hyeon-Ok
    • Journal of the Korean Society of Fisheries and Ocean Technology
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    • v.40 no.2
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    • pp.115-125
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    • 2004
  • An experiment to measure the volume variation of bag net in a set-net by acoustic telemetry system was conducted in Jaran Bay, Gosung, Korea on 10 April to 23 April 2003. The long baseline telemetry system consists of three radio-acoustic linked positioning (RAP) buoys, a time controller with a personal computer and seven pingers. Six pingers were attached on the bottom of the bag-net and the other one was fixed on the sea bed. The results obtained are summarized as follows : 1. The average RAP buoy fixing errors of x-axis, y-axis, and z-axis were 0.2m, 0.4m, and 0.1m, respectively. 2. In the neap tide the minimum and maximum volume of the bag-net on 11 April 2003 were 4,173$m^3$(17:00) and 4,757$m^3$(12:00), respectively. The average current direction and speed at those times were 99.9$^{\circ}$, 12.9cm/s and 104.0$^{\circ}$, 2.4cm/s, respectively. 3. In the spring tide on 17 April 2003, the minimum and maximum volume were 2,016$m^3$(18:30) and 4,454$m^3$(15:00), respectively. The average current direction and speed at those times were 315.6$^{\circ}$, 16.1cm/s and 289.0$^{\circ}$, 5.7cm/s, respectively. 4. In conclusion the maximum variation of the volume on 17 April to 20 April 2003 was 3,552$m^3$ and it was larger 1.4 times than time on 11 April to 16 April 2003.

Effect of Glycopyrrolate on Cardiovascular System in Dogs Sedated with Medetomidine-Midazolam Combination (개에서 Medetomidine과 Midazolam 병용 투여 시 Glycopyrrolate가 심맥관계에 미치는 영향)

  • Han, Dae-Kyung;Shin, Beom-Jun;Lee, Jae-Yeon;Jee, Hyun-Chul;Park, Ji-Young;Kim, Myung-Cheol;Jeong, Seong-Mok
    • Journal of Veterinary Clinics
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    • v.26 no.4
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    • pp.317-323
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    • 2009
  • This study was performed to evaluate the effect of intravenous administration of glycopyrrolate on cardiovascular and respiratory system in dogs given intravenous medetomidine (20 ${\mu}g$/kg) and intramuscular midazolam (0.3 mg/kg) (MM). Prior to administration of MM, glycopyrrolate was administered intravenously at doses of 5 ${\mu}g$/kg (Gly-5), 10 ${\mu}g$/kg (Gly-10) or 20 ${\mu}g$/kg (Gly-20), respectively. For the control group saline was administered intravenously. In the cardiovascular system, HR, BP, RAP, PAWP, CI, SI, SVR, and PVR were measured. RR, $V_T$, $P_{ETCO2}$, and arterial blood gas analysis were measured for respiratory system. Although rapid and satisfied depth of sedation was obtained by MM, life-threatening bradycardia, the outstanding side-effect on cardiovascular system in dogs were observed. This combination also decreased CO and increased SVR, RAP, and PAWP significantly. The bradycardia could be prevented in all the glycopyrrolate treated groups, but tachycardia was observed in Gly-10 and Gly-20 groups. Significant increases in blood pressure were shown in glycopyrrolate treated groups. Also, tachycardia depends on dose of glycopyrrolate, compensating the CO. However, these were not fully reserved. In conclusion, MM combination could induce rapid and satisfied depth of sedation but was not the suitable method for the deep sedation of dogs with cardiovascular or circulatory problems.