• Title/Summary/Keyword: R40

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Analysis of Ginsenosides of White and Red Ginseng Concentrates (백삼 및 홍삼 농축액의 사포닌 분석)

  • Ko, Sung-Kwon;Lee, Chung-Ryul;Choi, Yong-Eui;Im, Byung-Ok;Sung, Jong-Hwan;Yoon, Kwang-Ro
    • Korean Journal of Food Science and Technology
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    • v.35 no.3
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    • pp.536-539
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    • 2003
  • Commercial white and red ginseng concentrates were analysed for total ginsenoside contents, and compositions of ginsenosides $Rb_1,\;Rb_2,\;Rc,\;Re,\;Rf,\;Rg_1,\;20(S)\;Rg_3,\;20(S)\;Rh_1,\;and\;20(R)\;Rh_1$. The content of crude saponin and total ginsenosides of white ginseng concentrates (WGC) were about 2-3 times higher than those of red ginseng concentrates (RGC). HPLC showed that each ginsenoside content was higher in WGC, with those of $Rb_1,\;Rg_1,\;and\;Rb_2$ being over three times higher than that of RGC. 20(S)- and 20(R)-ginsenoside $Rg_3$, specific artifacts found only in red ginseng, were detected both in WGC and RGC by HPLC. differences in the contents of these specific ginsenosides between WGC and RGC were not significant. The contents of 20(S)-ginsenoside $Rg_1$, determined by HPLC were 0.40 and 0.53 in WGC, whereas 0.48% and 0.47%, and those of 20(R)-ginsenoside $Rg_3$, were 0.14 and 0.22% in WGC, and 0.10 and 0.11% in RGC using the methods of shibata and food Code, respectively.

THE MICROHARDNESS AND THE DEGREE OF CONVERSION OF LIGHT CURED COMPOSITE RESIN AND DUAL CURED RESIN CEMENTS UNDER PORCELAIN INLAY (도재인레이 하방에서 광중합형 복합레진과 이중중합형 복합레진시멘트의 미세경도와 중합률에 관한 연구)

  • Kim, Seung-Soo;Cho, Sung-Sik;Um, Chung-Moon
    • Restorative Dentistry and Endodontics
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    • v.25 no.1
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    • pp.17-40
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    • 2000
  • Resin cements are used for cementing indirect esthetic restorations such as resin or porcelain inlays. Because of its limitations in curing of purely light cured resin cements due to attenuation of the curing light by intervening materials, dual cured resin cements are recommended for cementing restorations. The physical properties of resin cements are greatly influenced by the extent to which a resin cures and the degree of cure is an important factor in the success of the inlay. The purpose of this study was to evaluate the influence of porcelain thickness and exposure time on the polymerization of resin cements by measuring the microhardness and the degree of conversion, to investigate the nature of the correlation between two methods mentioned above, and to determine the exposure time needed to harden resin cements through various thickness of porcelain. The degree of resin cure was evaluated by the measurements of microhardness [Vickers Hardness Number(VHN)] and degree of conversion(DC), as determined by Fourier Transform Infrared Spectroscopy(FTIR) on one light cured composite resin [Z-100(Z)] and three dual cured resin cements [Duo cement(D), 3M Resin cement(R), and Dual cement(DA)] which were cured under porcelain discs thickness of 0mm, 1mm, 2mm, 3mm with light exposure time of 40sec, 80sec, 120sec, and regression analysis was performed to determine the correlation between VHN and DC. In addition, to determine the exposure time needed to harden resin cements under various thickness of porcelain discs, the changes of the intensity of light attenuated by 1mm, 2mm, and 3mm thickness of porcelain discs were measured using the curing radiometer. The results were obtained as follows ; 1. The values of microhardness and the degree of conversion of resin cements without intervening porcelain discs were 31~109VHN and 51~63%, respectively. In the microhardness Z was the highest, followed by R, D, DA. In the degree of conversion, D and DA was significantly greater than Z and R(p<0.05). 2. The microhardness and the degree of conversion of the resin cements decreased with increasing thickness of porcelain discs, and increased with increasing exposure time, D and R showed great variation with inlay thickness and exposure time, whereas, DA showed a little variation. 3. The intensity of light through 1mm, 2mm, and 3mm porcelain inlays decreased by 0.43, 0.25, and 0.14 times compared to direct illumination, and the respective needed exposure times are 53 sec, 70 sec, and 93 sec. In D and R, 40 sec of light irradiation through 2mm porcelain disc and 80 sec of light irradiation through 3mm porcelain disc were not enough to complete curing. 4. The microhardness and the degree of conversion of the resin cements showed a positive correlationship(R=0.791~0.965) in the order of R, D, Z, DA. As the thickness of porcelain discs increased, the decreasing pattern of microhardness was different from that of the degree of conversion, however.

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Comparative Study of Acticoat® & Allevyn® on Infected Full-thickness Wound of the Rat Skin (흰쥐의 감염된 전층피부결손에 미치는 은 함유 드레싱제재와 폴리우레탄 폼 드레싱제재의 비교관찰)

  • Gok, Nak Soo;Kim, Han Koo;Kim, Seung Hong;Kim, Woo Seob;Bae, Tae Hee;Kim, Mi Kyung
    • Archives of Plastic Surgery
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    • v.34 no.2
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    • pp.169-175
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    • 2007
  • Purpose: $Allevyn^{(R)}$(Smith & Nephew, England) is a type of polyurethane foam material with good wound discharge absorption. $Acticoat^{(R)}$(Smith & Nephew, England) is a silver coated dressing material which is effective in infected wound. The purpose of this study is to compare the effects of dry gauze, $Acticoat^{(R)}$ and $Allevyn^{(R)}$ on infected full-thickness wound healing in rat. Methods: One hundred and twenty rats were divided into 3 groups: group I(dressing with dry gauze, n=40), group II(dressing with $Allevyn^{(R)}$, n=40), group III(dressing with $Acticoat^{(R)}$, n=40). A $15{\times}15mm$ square full-thickness wound was made on the dorsum and left open for 24 hours. The size of wound defects were measured each dressing changes. The histological evaluation was performed on the 3rd day, 7th day, 14th day, 21th day. Results: After the wound was left open for 24 hours, typical findings of bacterial infection was observed. After the 7th day, group III showed larger area of epithelialization, smaller defect size compared to those of two other groups. Complete replacement by fibrotic scar tissue was observed in group III with no signs of inflammation on the 14th day. By day 21, the average defect size in group III was decreased from initial 100% to 3.63%. while in group I and II, it was decreased to 62.66% and 53.62%, respectively. Conclusion: $Acticoat^{(R)}$ is an effective tool in the treatment of infected wound.

Study on Anti-oxidant and Anti-inflammatory Activity of the Tissue-cultured Shoot Clumps from Raoulia australis (라울리아 신초 추출물의 항산화 및 항염 효능에 관한 연구)

  • Park, Chang-Min;Han, Na-Kyeong;Joung, Min-Seok;Paek, Kee-Yoeup;Choi, Jong-Wan
    • Journal of the Society of Cosmetic Scientists of Korea
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    • v.40 no.4
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    • pp.349-357
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    • 2014
  • In this study, the shoot clumps extract of tissue-cultured Raoulia australis using the bioreactor culture system was tested for use a natural cosmetic ingredient. Tissue-cultured R. australis shoot clumps extract was tested anti-oxidant and anti-inflammatory activity for a cosmetic application. R. australis is a wild herbaceous plant of the asteraceae growing in New Zealand and Australia. Previous studies have reported anti-viral activity of the inhibitory effects for the growth of viruses induced meningitis, bronchitis and respiratory diseases but other biological effects are unknown. The shoot clumps extract of tissue-cultured R. australis showed higher anti-oxidant effect and anti-inflammatory effect than the natural R. australis extract. In DPPH, NBT and ABTS assay, the shoot clumps extract of tissue-cultured R. australis enhanced radical scavenging activity (up to 10~25% at $50{\mu}L/mL$) more than the natural R. australis extract. Also, the shoot clumps extract of tissue-cultured R. australis inhibited expression of iNOS and COX-2 protein in LPS-stimulated Raw 264.7 macrophages more than the natural R. australis extract. From this study, the shoot clumps extract of tissue-cultured R. australis displayed strong possibility as a new natural cosmetic ingredient for skin-care products.

Molecular Analysis of Complete SSU to LSU rDNA Sequence in the Harmful Dinoflagellate Alexandrium tamarense (Korean Isolate, HY970328M)

  • Ki, Jang-Seu;Han, Myung-Soo
    • Ocean Science Journal
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    • v.40 no.3
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    • pp.155-166
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    • 2005
  • New PCR primers (N=18) were designed for the isolation of complete SSU to LSU rDNA sequences from the dinoflagellate Alexandrium tamarense. Standard PCR, employing each primer set selected for amplifications of less than 1.5 kb, successfully amplified the expected rDNA regions of A. tamarense (Korean isolate, HY970328M). Complete SSU, LSU rDNAs and ITS sequences, including 5.8S rDNA, were recorded at 1,800 bp, 520 bp and 3,393 bp, respectively. The LSU rDNA sequence was the first report in Alexandrium genus. No intron was found in the LSU rRNA coding region. Twelve D-domains within the LSU rDNA were put together into 1,879 bp (44.4% G+C), and cores into 1514 bp (42.8% G+C). The core sequence was significantly different (0.0867 of genetic distance, 91% sequence similarity) in comparison with Prorocentrum micans (GenBank access. no. X16108). The D2 region was the longest in length (300 bp) and highly variable among the 12 D-domains. In a phylogenetic analysis using complete LSU rDNA sequences of a variety of phytoplankton, A. tamarense was clearly separated with high resolution against other species. The result suggests that the sequence may resolve the taxonomic ambiguities of Alexandrium genus, particularly of the tamarensis complex.

Studies on the Lactation Curve of Holstein Cows in Gwangju Area (광주지방 유우의 비유곡선)

  • 나진수;문승주
    • Korean Journal of Animal Reproduction
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    • v.6 no.1
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    • pp.31-35
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    • 1982
  • A study of 188 lactations of Holstein cows in Gwangju area was undertaken to establish the shape of lactation curve during the period from October in 1980 to January in 1982. The Gammafunction described by Wood(1967) was fitted to the lactations observed. The results obtained in the present study were summarized as follows; 1. The lactation curve of the 188 lactations was expressed by the equation based on Wood's model (1967) as follows; Yn=24.5m0.0762e-0.0944n(R2=0.99) 2. The lactation curves by parity were represented by the equations as follows; Yn=18.81n0.1486e-0.0741n(R2=0.98)……………parity 1 Yn=26.51n0.1161e-0.1200n(R2=0.96)……………parity 2 Yn=26.95n0.2804e-0.1703n(R2=0.99)……………parity 3 Yn=27.92n0.1429e-0.1427n(R2=0.98)……………parity 4 Yn=22.61n0.1985e-0.1211n(R2=0.94)……………parity 5 3. The lactation curves by calving seasons were represented by the equationes as follows; Yn=27.05n0.0739e-0.1005n(R2=0.98)……………spring Yn=23.08n0.2040e-0.1202n(R2=0.98)……………summer Yn=26.81n0.0460e-0.1134n(R2=0.98)……………autumn Yn=23.40n0.1299e-0.1101n(R2=0.95)……………winter

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Distribution of AMPA Glutamate Receptor GluR1 Subunit-immunoreactive Neurons and their Co-Localization with Calcium-binding Proteins and GABA in the Mouse Visual Cortex

  • Kim, Tae-Jin;Ye, Eun-Ah;Jeon, Chang-Jin
    • Molecules and Cells
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    • v.21 no.1
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    • pp.34-41
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    • 2006
  • The neuronal localization of alpha-amino-3-hydroxyl-5-methyl-4-isoxazole propionic acid (AMPA) glutamate receptor (GluR) subunits is vital as they play key roles in the regulation of calcium permeability. We have examined the distribution of the calcium permeable AMPA glutamate receptor subunit GluR1 in the mouse visual cortex immunocytochemically. We compared this distribution to that of the calcium-binding proteins calbindin D28K, calretinin, and parvalbumin, and of GABA. The highest density of GluR1-immunoreactive (IR) neurons was found in layers II/III. Enucleation appeared to have no effect on the distribution of GluR1-IR neurons. The labeled neurons varied in morphology; the majority were round or oval and no pyramidal cells were labeled by the antibody. Two-color immunofluorescence revealed that 26.27%, 10.65%, and 40.31% of the GluR1-IR cells also contained, respectively, calbindin D28K, calretinin, and parvalbumin. 20.74% of the GluR1-IR neurons also expressed GABA. These results indicate that many neurons that express calcium-permeable GluR1 also express calcium binding proteins. They also demonstrate that one fifth of the GluR1-IR neurons in the mouse visual cortex are GABAergic interneurons.

MicroRNAs as Promising Biomarkers for Tumor-staging: Evaluation of MiR21 MiR155 MiR29a and MiR92a in Predicting Tumor Stage of Rectal Cancer

  • Yang, Yun;Peng, Wei;Tang, Tian;Xia, Lin;Wang, Xiao-Dong;Duan, Bao-Feng;Shu, Ye
    • Asian Pacific Journal of Cancer Prevention
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    • v.15 no.13
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    • pp.5175-5180
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    • 2014
  • Objective: In this study, tumor-stage predictive abilities of miR21, miR155, miR29a and miR92a were evaluated in rectal cancer (RC). Methods: Expression of miR21, miR155, miR29a and miR92a was detected and quantitated in tumor tissue and in adjacent normal tissue from 40 patients by TaqMan MicroRNA assay. Results: Significant overexpression of miR21, miR155, miR29a and miR92a was observed in RC tissues. While high expression of miR21, miR155 and miR29a in N1-2 and C-D stages presented a potential correlation with N and Duke stages, partial correlation analysis suggested that only miR155 rather than miR21 and miR29a played a greater influencing role. Receiver operating characteristics (ROC) curve analysis showed that miR155 could discriminate N0 from N1-2 with 85.0% sensitivity and 85.0% specificity, N2 from N0-1 with 90.0% sensitivity and 96.7% specificity, and C-D stage from A-B stage with 81.0% sensitivity and 84.2% specificity. Conclusions: Increase in expression of miR155 might represent a novel predictor for RC N and Dukes staging.

Drop-In Evaluation of Thermodynamic Performance of R-22 Alternative Refrigerant Mixtures (R-22 대체용 혼합냉매의 Drop-In 열역학적 성능 계산)

  • Ju, J.M.;Kim, C.N.;Park, Y.M.
    • Korean Journal of Air-Conditioning and Refrigeration Engineering
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    • v.8 no.3
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    • pp.423-436
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    • 1996
  • Thermodynamic performance of eight zeotropic R-22 alternative refrigerant mixtures selected by AREP(R-22 Alternative Refrigerants Evaluation Program) and R-32/R-125/R-134a(23%/25%/52%), namely R-407C were evaluated by the "drop-in" simulation method. An existing air conditioner was selected and its design data were used for the simulation. "ARI Test A" air conditions were applied. The degree of vapor superheat at the compressor inlet fixed at $5^{\circ}C$ for all the mixtures. The results of the simulation were compared with those of R-22. COPs of all mixtures except for R-32/R-227ea(35%/65%) and R-32/R-125/R-134a(10%/70%/20%), were higher than that of R-22 by 2%~8%, while the capacities were all lower than that of R-22 by 13%~27%. COP of R-32/R-134a(40%/60%) was 2.4% higher but the capacity was 15% lower than those of R-22. In the case of R-32/R-134a(30%/70%), COP and capacity were 5.5% higher and 15% lower than those of R-22, respectively. Among the ternary mixtures, R-407C and R-32/R-125/R-134a(30%/10%/60%) showed the best performance. COP of R-407C was 2.4% higher than those of R-22 but the capacity was 15% lower.

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Characterization of Oil-Degradation Biosurfactant Produced by Bacillus sp. TBM40-3 (Bacillus sp. TBM40-3에 의해 생성된 Biosurfactant의 유류분해 특성)

  • Kim, Sun-Hee;Lee, Sang-Cheol;Yoo, Ju-Soon;Joo, Woo-Hong;Chung, Soo-Yeol;Choi, Yong-Lark
    • Applied Biological Chemistry
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    • v.47 no.2
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    • pp.170-175
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    • 2004
  • In this paper we studied about Bacillus sp. TBM40-3 producing biosurfactants. The strains were isolated from Taeback Mountain soil and identified as Bacillus sp. by l6S rDNA nucleotides sequence analysis. The TBM40-3 was gram-positive and rod-shaped as observed by field emission scanning microscopy. After the cultivation TBM40-3 in LB broth for 90 h and the surface tension of supernatant was decreased to 29 mN/m. Emulsification activity and stability of crude biosurfactant was measured by using water-immiscible hydrocarbons and oil as substrate. Maximum emulsification activity and stability was obtained from soybean oil. Also, we confirmed that the TBM40-3 producing biosurfactant had an effect on crude oil while showing a superior effect as compared to chemically synthesized surfactants (SDS, Span85, Tween40, Triton X-100). As a result, the Bacillus sp. TBM40-3 producing biosurfactant had potent properties as an emulsifying agent and an emulsion stabilizing agent.