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Characterization of Oil-Degradation Biosurfactant Produced by Bacillus sp. TBM40-3  

Kim, Sun-Hee (Department of Biotechnology Faculty of Natural Resources and Life Science, Dong-A University)
Lee, Sang-Cheol (Department of Biotechnology Faculty of Natural Resources and Life Science, Dong-A University)
Yoo, Ju-Soon (Department of Biotechnology Faculty of Natural Resources and Life Science, Dong-A University)
Joo, Woo-Hong (Dept. of Biology, Chang-Won University)
Chung, Soo-Yeol (Dept. of Food Science, Dong-Ju College)
Choi, Yong-Lark (Department of Biotechnology Faculty of Natural Resources and Life Science, Dong-A University)
Publication Information
Applied Biological Chemistry / v.47, no.2, 2004 , pp. 170-175 More about this Journal
Abstract
In this paper we studied about Bacillus sp. TBM40-3 producing biosurfactants. The strains were isolated from Taeback Mountain soil and identified as Bacillus sp. by l6S rDNA nucleotides sequence analysis. The TBM40-3 was gram-positive and rod-shaped as observed by field emission scanning microscopy. After the cultivation TBM40-3 in LB broth for 90 h and the surface tension of supernatant was decreased to 29 mN/m. Emulsification activity and stability of crude biosurfactant was measured by using water-immiscible hydrocarbons and oil as substrate. Maximum emulsification activity and stability was obtained from soybean oil. Also, we confirmed that the TBM40-3 producing biosurfactant had an effect on crude oil while showing a superior effect as compared to chemically synthesized surfactants (SDS, Span85, Tween40, Triton X-100). As a result, the Bacillus sp. TBM40-3 producing biosurfactant had potent properties as an emulsifying agent and an emulsion stabilizing agent.
Keywords
Bacillus sp. TBM40-3; biosurfactant; emulsification activity; emulsification stability;
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