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http://dx.doi.org/10.7314/APJCP.2014.15.13.5175

MicroRNAs as Promising Biomarkers for Tumor-staging: Evaluation of MiR21 MiR155 MiR29a and MiR92a in Predicting Tumor Stage of Rectal Cancer  

Yang, Yun (Department of Gastrointestinal Surgery, West China Hospital)
Peng, Wei (West China Medical School, Department of Hepatic Surgery and Hepatic Transplantation Center, West China Hospital)
Tang, Tian (West China Medical School, Department of Gynecology and Obstetrics, West China Women's and Children's Hospital)
Xia, Lin (Department of Gastrointestinal Surgery, West China Hospital)
Wang, Xiao-Dong (Department of Gastrointestinal Surgery, West China Hospital)
Duan, Bao-Feng (Department of Oncology, West China Hospital, Sichuan University)
Shu, Ye (Department of Gastrointestinal Surgery, West China Hospital)
Publication Information
Asian Pacific Journal of Cancer Prevention / v.15, no.13, 2014 , pp. 5175-5180 More about this Journal
Abstract
Objective: In this study, tumor-stage predictive abilities of miR21, miR155, miR29a and miR92a were evaluated in rectal cancer (RC). Methods: Expression of miR21, miR155, miR29a and miR92a was detected and quantitated in tumor tissue and in adjacent normal tissue from 40 patients by TaqMan MicroRNA assay. Results: Significant overexpression of miR21, miR155, miR29a and miR92a was observed in RC tissues. While high expression of miR21, miR155 and miR29a in N1-2 and C-D stages presented a potential correlation with N and Duke stages, partial correlation analysis suggested that only miR155 rather than miR21 and miR29a played a greater influencing role. Receiver operating characteristics (ROC) curve analysis showed that miR155 could discriminate N0 from N1-2 with 85.0% sensitivity and 85.0% specificity, N2 from N0-1 with 90.0% sensitivity and 96.7% specificity, and C-D stage from A-B stage with 81.0% sensitivity and 84.2% specificity. Conclusions: Increase in expression of miR155 might represent a novel predictor for RC N and Dukes staging.
Keywords
Oncogenic microRNAs; rectal cancer; N stage; Dukes stage; miR21; miR155; miR29a; miR92a;
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