• Microbiology and Biotechnology Letters
• /
• v.30 no.2
• /
• pp.151-156
• /
• 2002

This study was performed to obtain the thermostable $\beta$-glycosidase producing bacteria from hot spring of volcanic area at Atagawa in Japan. KNOUC 202 was selected because it showed thermostable $\beta$-glycosidase activity in sodium phosphate buffer(pH 6.8) at $70^{\circ}C$ for 4h, and it was identified. The strain was aerobic, asporogenic bacilli, immobile, gram negative, catalase positive, oxidase positive, and pigment-producing. Optimum growth was at $70~72^{\circ}C$, pH 7.0~7.2, and it could grow in the presence of 3% NaCl. The main fatty acids in cell were iso-15:0 and iso-l7:0. 16S rRNA sequence of KNOUC 202 showed 99.9% similarity with that of Thermus thermophilus ATCC 27634(HB8). Based on morphological, physiological, biochemical characteristics, cellular fatty acids profile and 16S rRNA sequence analysis, KNOUC 202 was identified as Thermus thermophilus.

• 대한핵의학회:학술대회논문집
• /
• 1996.05a
• /
• pp.202-202
• /
• 1996

• Childhood Kidney Diseases
• /
• v.3 no.2
• /
• pp.209-216
• /
• 1999

Purpose : Nephrogenic diabetes insipidus (NDI) is a rare X-linked disorder associated with renal tubule resistance to arginine vasopressin (AVP). The hypothesis that the defect underlying NDI might be a dysfunctional renal AVPR2 has recently been proven by the identification of mutations in the AVPR2 gene in NDT patients. To investigate the association of mutations in th AVPR2 gene with NDI, we analyzed the AVPR2 gene located on the X chromosome. Methods : We have analyzed the AVPR2 gene in a kindred with X-linked NDI. The proband and proband's mother were analyzed by polymerase chain reaction-single strand conformational polymorphism(PCR-SSCP) and DNA sequencing of the AVPR2 gene. We also have used restriction enzyme analysis of genomic PCR product to evaluate the AVPR2 gene. Results : C to T transition at codon 202, predictive of an exchange of tryptophan 202 by cysteine(R202C) in the third extracellular domain was identified. This mutation causes a loss of Hae III site within the gene. Conclusion : We found a R202C missense mutation in the AVPR2 gene causing X-linked NDI, and now direct mutational analysis is available for carrier screening and early diagnosis.

• Korean Journal of Soil Science and Fertilizer
• /
• v.48 no.5
• /
• pp.485-491
• /
• 2015

This study was performed to investigate thermophilic bacteria from soil having broad antifungal spectrum against Rhizoctonia solani, Colletotrichum gloeosporioides, Phytophthora capsici, Fusarium oxysporum f.sp. lycopersici, and Botrytis cinerea. One isolate selected could resist heat shock of $60^{\circ}C$ for one hour, and had broad antifungal activity in dual culture assay against all tested fungal pathogens and was identified as Bacillus amyloliquefaciens Y1 using 16S rRNA gene sequence. Further investigation for antifungal activity of bacterial culture filtrate (BCF) and butanol crude extract (BCE) of various concentrations showed broad spectrum antifungal activity and fungal growth inhibition significantly increased with increasing concentration with highest growth inhibition of 100% against R. solani with 50% BCF and 11 mm of zone of inhibition against R. solani with 4 mg BCE concentration. Treatment of butanol crude extract resulted in deformation, lysis or degradation of C. gloeosporioides and P. capsici hyphae. Furthermore, B. amyloliquefaciens Y1 produced volatile compounds inhibiting growth of R. solani (70%), C. gloeosporioides (65%) and P. capsici (65-70%) when tested in volatile assay. The results from the study suggest that B. amyloliquefaciens Y1 could be a biocontrol candidate to control fungal diseases in crops.

• Kyungpook Mathematical Journal
• /
• v.32 no.2
• /
• pp.197-202
• /
• 1992

• Korean Journal of Air-Conditioning and Refrigeration Engineering
• /
• v.28 no.5
• /
• pp.202-207
• /
• 2016

Experimental studies for an indirect R404A-$CO_2$ refrigeration system and a direct R404A refrigeration system were conducted. The configurations of the indirect R404A-$CO_2$ refrigeration system are a R404A refrigeration system as a top cycle and a circulating $CO_2$ system as a bottom cycle. The direct R404A system was modified from indirect R404A-$CO_2$ refrigeration system by removing circuit for $CO_2$ circulation. Various tests for both systems were conducted by changing load side brine temperature from 0 to 5 and $10^{\circ}C$ with cooling brine temperatures for R404A system at 15, 20, or $25^{\circ}C$. The indirect R404A-$CO_2$ refrigeration system showed the highest COP when load side brine temperature was at $10^{\circ}C$ in the evaporator and at cooling brine temperature of $15^{\circ}C$. The COP of 3.04 under that condition was the highest. This indirect R404A-$CO_2$ refrigeration system showed 9.02% higher COP than the direct R404A system that had increased pipeline length of 15 m, which simulated actual installation in a supermarket.

• Journal of Korea Society of Industrial Information Systems
• /
• v.28 no.4
• /
• pp.45-58
• /
• 2023

In a world of uncertainty and complexity, leadership is essential to lead collaborative and positive interactions among employees. In other words, if members share opinions and work through voluntary leadership, they will respond more effectively to uncertain challenges and get closer to the targeted management performance. Therefore, in this study, we would like to elucidate the importance of shared leadership, which has recently become an issue. We will examine the impact of shared leadership on team efficacy, team organizational citizenship behavior, and turnover intention. A survey was conducted among members working in a team organization in Busan, and the results were as follows. First, the effects of shared leadership on team efficacy were found to have significant positive(+) effects, such as the hypotheses set at planning and organizing 0.202(C.R.=2.853), problem solving 0.463(C.R.=5.620), support and caring 0.237(C.R.=3.326), and development and mentoring 0.366(C.R.=5.132), respectively. Second, the effects of team efficacy on team organizational citizenship behavior and turnover intention were 0.545(C.R.=5.895) and -0.143(C.R.=-0.817), respectively, and team efficacy was found to have a positive(+)positive(+) effect on team organizational citizenship behavior, but team efficacy did not have a significant effect on turnover intention.

• Korean Journal of Plant Resources
• /
• v.11 no.2
• /
• pp.202-209
• /
• 1998

Through total fatty acid analysis by the Gas chromatography, 17 different fatty acids were identified from the sap extracted from bark of Korean Rhus(Anacardiaceae), six species. Linoleic acid(C18 :2) and oleic acid(C18 : 1) were identified as major fatty acids for the species investigated, but except in R. chinensis.In the fatty acid composition for the species, one or two specific-fatty acid was detected, and its could be utilized as a useful taxonomic character.The result of cluster analysis by the 22 reliable characters in the fatty acid composition suggested first group was R.trichocarpa and R. succedanea, the second group, R.verniciflua, R.ambgua and R. sylvestris, but R.chinensis was a greatly independent species. The possible chemotaxonomic application of the fatty acid composition of bark in the genus Rhus was discussed.

• Korean Journal of Food Science and Technology
• /
• v.44 no.2
• /
• pp.202-206
• /
• 2012

In order to prepare rice vinegar, $takju$ fermentation was performed, using two different compositions; cooked rice supplemented with $Rosa$ $rugosa$ Thunb. (CR-R) and uncooked rice supplemented with $Rosa$ $rugosa$ Thunb. (UR-R). After ethanol fermentation, ethanol contents of $takju$ were adjusted to 6.0%, and then acetic acid fermentation was carried out using $Acetobacter$ $aceti$ at $30^{\circ}C$ with 200 rpm for 9 days. Supplementations of 1% acetic acid to $takju$ prior to acetic acid fermentation were a critical step in achieving a successful fermentation; final organic acid contents increased in a dose dependent manner. A higher content of organic acids, 1.6-6.9%, was found in UR-R vinegar. The level of free amino acid and GABA in UR-R vinegar was higher than that of CR-R vinegar, with glutamic acid and arginine, as the major amino acids. This finding demonstrates that heating process of rice prior to ethanol fermentation affects the quality of vinegar significantly.

• The Korean Journal of Ceramics
• /
• v.1 no.3
• /
• pp.143-146
• /
• 1995

Twins along(202) and (211) planes are observed in monoclinic $ZrO_2$ thin film, which is deposited on Si substrate by MOCVD at $350^{\circ}C$ and annealed at $1150^{\circ}C$ for 10 hours in air. These types of twin have not been reported in monoclinic $ZrO_2$. The twins seem to be originated from the two dimensional tensile stresses applied to the $ZrO_2$ thin film due to the different thermal expansions of $ZrO_2$ thin film and Si substrate.