• Title/Summary/Keyword: R-peak

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Phosphorylated Proteins of Mitogen Stimulated-Rat Peripheral Blood Lymphocytes (분열유발인자에 의한 흰쥐 림프구 단백의 인산화)

  • Jou, I-Lo;Ko, Sung-Soo;Ahn, Young-Soo
    • The Korean Journal of Pharmacology
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    • v.29 no.1
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    • pp.121-130
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    • 1993
  • This study was done to classify the proteins involved in the specific phosphorylation using the rat peripheral blood lymphocytes (rPBL) stimulated with mitogens, phorbol 12-myristate 13-acetate (PMA) and concanavalin A (Con A). The lymphocytes were incubated with $^{32}P-orthophosphate$ before PMA or Con A stimulation. The migration patterns of the phosphorylated proteins of mitogen-treated rPBL in two dimensional electrophoretic fields were analyzed after autoradiography. The stimulation of the lymphocytes with PMA and Con A increased the phosphorylation of thirteen protein fractions. The phosphorylation intensities of the protein spots differ to the treatments of the cells with specific kinase inhibitors, H-7 and W-7. These protein fractions were grouped into 3 classes, namely, PKC-mediated, CaM kinase-mediated, and other kinase mediated proteins. The effect of the duration of the stimulation on the phosphorylated behaviors occurred concurrently, not sequentially, although each individual protein fraction had a different time for the peak phosphorylation during the stimulation period upto 30 minutes. The phosphoproteins found in the cytosolic soluble fraction were phosphorylated prior to those in the pellet, whose phosphorylations were sustained at a high level for over 10 minutes. The above results suggest that the early events in lymphocyte activation involve 3 different sets of proteins which are phosphorylated by CaM kinase, PKC and other kinases, and those kinases do not work sequentially, but rather, independently or cooperatively.

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Sedimentological and Hydromechanical Characteristics of Bed Deposits for the Cultivation of Manila clam, Ruditapes philippinarum in Gomso Tidal Flat (곰소만 조간대 바지락 양식장 저질의 퇴적학적 및 수리역학적 특성)

  • CHO Tae-Chin;LEE Sang-Bae;KIM Suck-Yun
    • Korean Journal of Fisheries and Aquatic Sciences
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    • v.34 no.3
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    • pp.245-253
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    • 2001
  • To investigate the effects of hydromechanical and textural characteristics of sediment deposits on the cultivation of Manila clam, Ruditapes philippinarum surface and sub-surface core sediments were collected seasonally in Gomso tidal flat. Grain size distribution were analyzed to investigate the annual variation of sediment texture. In winter unimodal distribution of grain size with the peak at $5\phi$ is dominant However, during the summer sediment texture become a little bit coarser and grain size distribution shows the peaks at $4\~5 \phi$. Optimum sediment texture for the cultivation of manila clam, R. philippinarum was found to be sandy silt in which mean Brain size was between 4 and $5 \phi$ with the sand content less than $50\%$ and clay content of $5\~10\%$. Mechanical and hydrological characteristics of sediment deposits were also studied in the laboratory and the results were applied to the numerical simulation for the behavior of surface sediment subjected to the cyclic loading from sea-water level change. Results of numerical simulation illustrate that the permeability of sediment had to be maintained in the range of $10^{-11}\sim10^{-12}m^2$ to ensure the proper sedimentological environment for the cultivation of manila clam, R. philippinarum. The deposits of virtually impermeable mud layer, with the threshold thickness of 4 cm, would be very hazardous to clam habitat.

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Studios on the Synthetic Pheromones of Striped Rice Borer and Tortricid Insect Pests (이화명충과 과수잎말이나방류 해충의 합성 Pheromone에 관한 연구)

  • Song Y. H.;Song H. Y.;Kim H. K.;Chang Y. D.;Lippold P. C.
    • Korean journal of applied entomology
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    • v.17 no.1 s.34
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    • pp.41-47
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    • 1978
  • Pheromones of several insect species were evaluated in a screening program in terms of their usefulness in pest forecasting and control in Korea. Species included striped rite borer (Chilo suppressalis) and tortricid moths, which attack deciduous fruit, and colding moth. The pheromone of striped rice borer was supplied through the courtesy of the Tropical Products Institute, London. Pheromones of other species were obtained from Cornell University and the Zoecon Corporation of Palo Alto, California. 'rho results of this experiment were as follows: 1. Live traps containing virgin striped rice borer female moths were more effective in attracting male moths than were the pheromone traps. 2. Since the effectiveness of the striped rice borer pheromone decreased dramatically with time, it was difficult to estimate the peak tine of the borer emergence. 3. The primary species trapped in deciduous fruit orchards was the oriental fruit moth, Grapholitha molesta. The traps were baited with the phermones, OFM and LAW. 4. Several moth species were trapped with OBLR. RBLR. SPAR and ArcM phermones but few were trapped with the remaining eight tortricid pheromones. 5. The following tortricid pheromones might be useful for forecasting the species given: OFM, LAW : Grapholitha molesta OBLR : Archips breviprecanus OBLH, RBLR, ArcM : Archippus coreensis Archips fuscocupreanus Hoshinoa longicellana SPAR, TBM ; Phyroderces sp.

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Economic Injury levels of Liriomyza trifolii Burgess (Diptera: Agromyzidae) infesting Eggplant in Greenhouse (시설 가지에서 아메리카잎굴파리의 경제적피해수준)

  • Lim, Ju-Rak;Moon, Hyung-Cheol;Choi, Seon-U;Kim, Ju-Hee;Lee, Ki-Kwon;Ko, Bok-Rai;Choi, Jung-Sick;Jeon, Yong-Kyun;Hwang, Chang-Yeon
    • Korean journal of applied entomology
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    • v.46 no.3
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    • pp.409-414
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    • 2007
  • Economic injury levels and economic thresholds were estimated for the american serpentine leafminer (Liriomyza trifolii) on greenhouse eggplant. Liriomyza trifolii density was increased until the late June and decreased after the July in innoculation on may 17. Growth of an aerial plants and fruits were not different in treatment respectively. But total number of fruits and yields were decreased on higher inoculation density. Whereas the rate of yield loss was increased. The rates of damaged leaf by L. trifolii were increased on higher inoculation density and the peak was 65%. The number of commodity fruits and the rates of commodity fruit were become lower than non-treatment (72.2%). The rates of damaged leaf area were 5.3, 11.7, 19.7, 25.7% on inoculation densities and the rates of yield loss were 0.6, 4.8, 9.8, 14.7%, respectively. There existed close correlation between rate of yield loss and inoculation density (Y = 0.76779X + 0.298354, $R^2\;=\;0.9599$). Considerated of the results, the economic injury levels of L. trifolii on eggplant greenhouse was 6.1 adults per 4 plant and the economic thresholds was 4.9 adults per 4 plant.

Quality and Sensory Characteristics of White Bread added with Various Ginseng Products (인삼제품의 첨가에 따른 제빵적성 및 관능평가)

  • Song, Mi-Ran;Lee, Ka-Soon;Lee, Byeong-Chan;Oh, Man-Jin
    • Food Science and Preservation
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    • v.14 no.4
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    • pp.369-377
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    • 2007
  • This study investigated the effect of ginseng products on the baking properties of white breads. Flour was substituted by ginseng products (ginseng at levels 2, 4, 6, and 8%, all w/w, of flour levels). Both ginseng powder (GP) and red ginseng powder (RGP) were used. Similarly, red ginseng extract (RGE) was substituted at levels of 1, 2, 3, and 4% (all w/w) of flour. The physical properties of each dough were assessed using farinograms, extensograms, and amylograms. Water absorption increased as the proportions of ginseng products rose. The dough surface areas and R/E (resistance/extensibility) values decreased, as did peak viscosities, at the proportions of ginseng products increased. The pH values during fermentation decreased as the proportions of ginseng products increased. The fermentation power of dough with GP was lower than that of control, and higher than that of dough with RGP or RGE, but the addition of ginseng products beyond certain levels weakened the gas retention power: The specific loaf volumes of breads with 2% GP were the highest at 5.41 mL/g. In breads with RGE, the specificloaf volume increased from 5.52 mL/g to 5.82 mL/g as RGE levels rose from 0% to 4%. Hardness increased with rising GP and RGP levels in breads with GP and RGP, but decreased with RGE levels in breads with RGE. The moisture contents of breads during storage tended to be higher than control in breads with ginseng products. Lightness increased with addition of GP and decreased upon addition of RGP or RGE, while redness and yellowness increased after addition of any ginseng products. In sensory evaluation tests, the sensory scores for texture, color, mouth feel, and overall acceptability, were high, when any ginseng product (GP, RGP or RGE) was present at 2%. Of these breads, the bread with 2% RGE attained the highest sensory score.

Progesterone Production and Oocyte Maturatf on of Frog (Rona nigromaculata and Rana rugoBa) Follicles in vitro (참개구리와 옴개구리 여포의 프로제스테론 생성과 난자의 성숙)

  • 권혁방;김지열;고선근
    • The Korean Journal of Zoology
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    • v.33 no.2
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    • pp.175-182
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    • 1990
  • Progesterone production and oocyte maturation in ovarian follicles of Rana nigromaculata and Rana rugosa were investigated. Addition of frog pituitary homogenate (FPH) to the in utiro cultured follicles of R. nigromaculata stimulated a marked increase in the accumulation and secretion of progesterone (P$_4$) by the follicles and induced their oocyte maturation (germinal vesicle breakdown, GVBD) in a dose dependent manner. The FPH (0.1 pituitary equivalent/2 ml)-inducted P4 peak appeared in 3-6 hours and followed by the oocyte GVBD in 9-12 hours after the hormone stimulation. lncreae of intrafollicular cAMP levels with forskolin (an adenylatecyclase stimulator) and 3-isobutyl-1-methylxanthine (IBMX, a phosphodiesterase inhibitor) mimic the FPH action in the stimulation of P$_4$ production but not in the induction of oocyte maturation. The in uitro cultured follicies of R. rugosa behaved very differently from other amphibian follicles. Addition of FPH-(0. 1 pit. equivl2 ml) to the culture medium neither stimulated P$_4$ production by the follicles nor induced the oocyte GVBD. However, treatment of the follicles with forskolin and IBMX drastically stimulated both the intrafollicular accumulation (800 pg/follicle) and secretion (1700 pg/follicle) of P$_4$ by the follicles during culture period. Thus, the data suggest that the follicles are ready to respond to cAMP increase but not to the FPH stimulation in terms of P$_4$ production.

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Annealing Effect and Tunability of BaZr0.08Ti0.92O3 Polycrystal Grown in N2 Gas Atmosphere by Floating Zone Technique (Floating Zone Technique법으로 질소분위기 하에서 성장한 BaZr0.08Ti0.92O3 다결정의 Tunability 및 열처리 효과)

  • Hwang, Ho-Byong
    • Journal of the Korean Institute of Electrical and Electronic Material Engineers
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    • v.17 no.11
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    • pp.1178-1185
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    • 2004
  • In the atmosphere of $N_2$ gas, BaZ $r_{0.08}$ $Ti_{0.92}$ $O_3$ polycrystal was grown by floating zone technique using BaZ $r_{0.08}$ $Ti_{0.92}$ $O_3$ ceramics as a feed and SrTi $O_3$(1l0) single cystal as a seed. The dielectric constant and loss at 10 kHz, 100 kHz, and 1 MHz for the as-grown sample were measured as a function of temperature in the temperature range between -10$0^{\circ}C$ and 150 $^{\circ}C$ to find a dielectric peak with frequency dispersion at Curie point. The hysteresis loop showed that the grown sample had very small polarization which was 0-0.01 $\mu$C/$\textrm{cm}^2$ for the applied dc-electric fields from -7 kV/cm to +7 kV/cm. However, the normal hysteresis loop was appeared after oxygen annealing. The electric-field dependence of the dielectric constant for both the as-grown and the post-annealed samples was studied by measuring the dielectric constants as a function of the biased-electric fields and their tunability was figured out from it at room temperature(27 $^{\circ}C$) and cryotemperature( -73$^{\circ}C$). Tunability for the as-grown sample was 51 % and the figure of merit 20.4 at 10kHz with the biased electric-field of 12 kV/cm. The tunability for the grown sample may be increased up to 80 % if the electric field of 25 kV/cm is applied. Tunability for the post-annealed sample was 41 % and the figure of merit 10.3 at 10 kHz with the biased electric-field of 12 kV /cm. Post-annealing improved the crystallinity of the as-grown sample but decreased its tunability.ability.

FH DFT-Spreading OFDM System for the Effective Channel Estimation and PAPR Reduction in Jamming Channel (재밍 채널에서 효과적 채널 추정과 PAPR 저감을 위한 주파수 도약 DFT-Spreading OFDM 시스템)

  • Kim, Jang-Su;Ryu, Heung-Gyoon;Lee, Seung-Jun;Ko, Dong-Kuk
    • The Journal of Korean Institute of Electromagnetic Engineering and Science
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    • v.21 no.7
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    • pp.796-804
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    • 2010
  • It is important to use the comb type pilot allocation for the continuous channel and efficient processing. And DFT-spreading OFDM is used a lot to solve high PAPR problem of OFDM system. However, PAPR is increased again when comb type pilot is used to estimate channel characteristics. So, in this paper, we employ a new SLM method to DFT-spreading OFDM system to reduce increased high PAPR. And we suggest an effective method to transmit side information without additional bandwidth. Pilot and side information must be preserved from jamming or intentional interferences since those are very important in DFT spread OFDM system using SLM. So, in this paper, we like to analyze and simulate the performance of DFT spread OFDM system based on SLM against jamming signal. To remedy the vulnerable shortcomings of DFT spread OFDM about jamming or intentional interferences, we employ FH(Frequency Hopping) method and analyze system performance under the several jamming conditions such as MTJ(Multi Tone Jamming) and PBJ(Partial Band Jamming).

Prediction of Pine-mushroom (Tricholoma matsutake) Production from the Ratio of Each Grade at the Joint Market (공판되는 송이의 등급별 비율을 통한 향후 생산량 추이 예측)

  • Park, Hyun;Jung, Byung-Heon
    • Journal of Korean Society of Forest Science
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    • v.99 no.4
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    • pp.479-486
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    • 2010
  • We analyzed the relationships between the daily yield and quality of pine-mushroom to predict the annual production pattern and unit price of the mushroom with the records of pine-mushroom trade at Yeongdeok forestry cooperative's market for nine years (2000~2008). Although there were some exceptions due to extreme drought or extraordinary temperature, the production ratio of high quality (first and second grade) was more than 50% in early stage and decreased, while that of low quality (pileus opened and defected ones) showed increasing pattern after the production reached in peak. The ratio of high quality and that of low quality were reversed 1~9 days before the mushroom production reached the acme of daily yield, which allowed us to predict that the mushroom production would be decreased when the ratio of low quality overcomes that of high quality. The ratio of high quality preceded about 3~4 days prior to that of daily yield, and the mushroom yield showed significant correlations with the ratio of high quality mushroom prior to 3~4 days of the day with the coefficient larger than 0.5 (r=0.51 for 3 days and r=0.54 for 4 days). Thus, we concluded that the analysis of grade distribution of pine-mushroom at the market may provide a significant clue to predict production pattern of the mushroom. In addition, the price of high quality pine-mushroom showed clear negative correlations with the yield. Thus, the analysis may take a good role for the trading of pine-mushroom with providing information for predicting the price of pine-mushroom.

Quantitative Analysis and Qualification of Amitrole Using LC/ESI-MS (LC/ESI-MS를 이용한 아미트롤의 정성확인 및 정량분석)

  • Park, Chan-Koo;Eo, Soo-Mi;Kim, Min-Young;Sohn, Jong-Ryeul;Mo, Sae-Young
    • Analytical Science and Technology
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    • v.17 no.2
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    • pp.117-129
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    • 2004
  • Amitrole in environment, difficult to be analyzed by GC or GC/MS due to high polarity and low volatility, was analyzed by LC/ESI/MS in the study. Maximum peak intensity of amitrole in LC/MS/ESI mass spectrum is m/z 85 of protonated molecular ion $(M+H)^+$ with 30V of cone voltage at SIR mode. It was confirmed that ratios between main ion of amitrole, 85 of protonated molecular ion, and m/z 58 fragmented ion of amitrole, had increased corresponding with the increment of cone voltage from 20V to 70V. The isotope molecular weight of amitrole was $86([M+H])^+$ at LC/MS analysis and the mass spectrum ratio between 85 mass and 86 mass was not different by the change of concentration but similar to theoretical ratio(less than 10% standard deviation).The linearity of standard calibration curve under same condition with sample treatment method had $y=1.09354e^6X+26947.2$ and $r^2=0.99$. Recovery rates in water and soil samples were 77.64~83.44% and 71.11~79.44%, respectively. Reliability of the analysis was performed with 5 repeated measurements at each level of standard concentration and the result showed that relative standard deviation was less than 10%; therefore, the extraction and analysis method in the study suggested that it would be reliable to measure amitrole in water and soil media.