• Journal of Microbiology and Biotechnology
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• v.26 no.6
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• pp.1115-1123
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• 2016

_L-Asparaginase (E.C. 3.5.1.1) is an enzyme involved in asparagine hydrolysis and has the potential to effect leukemic cells and various other cancer cells. We identified the _L-asparaginase gene (_L-ASPG86) in the genus Mesoflavibacter, which consists of a 1,035 bp open reading frame encoding 344 amino acids. Following phylogenetic analysis, the deduced amino acid sequence of _L-ASPG86 (_L-ASPG86) was grouped as a type I asparaginase with respective homologs in Escherichia coli and Yersinia pseudotuberculosis. The _L-ASPG86 gene was cloned into the pET-16b vector to express the respective protein in E. coli BL21 (DE3) cells. Recombinant _L-asparaginase (r-_L-ASPG86) showed optimum conditions at 37-40℃, pH 9. Moreover, r-_L-ASPG86 did not exhibit glutaminase activity. In the metal ions test, its enzymatic activity was highly improved upon addition of 5 mM manganese (3.97-fold) and magnesium (3.35-fold) compared with the untreated control. The specific activity of r-_L-ASPG86 was 687.1 units/mg under optimum conditions (37℃, pH 9, and 5 mM MnSO₄).

• Journal of Labour Economics
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• v.34 no.1
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• pp.33-69
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• 2011

This study analyses a productivity effect by E&T and R&D activities via estimation of E&T stock, R&D stock, and patent stock in a corporate level. Particularly, the analysis reflects the effects of skilled training after estimating E&T stock from E&T flow. When a spillover effect of E&T is analyzed, a methodology using technical proximity concept becomes a new experiment. Also classifying long and short term effects from the usage of Dynamic Panel Data Analysis becomes a new trial, too. The results of study appear that the productivity effects from E&T investments are relatively lager than R&D investments. Through spillover effects and long-term effects E&T and R&D activities have a strong influence on the corporate's productivity.

• Communications of Mathematical Education
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• v.24 no.1
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• pp.145-158
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• 2010

In this paper we study tetrahedron's properties related with center of inscribed sphere using the center of mass. We show that the center of mass of four mass points (A,a), (B,b), (C,c), (D,d) coincide with center of tetrahedron's inscribed sphere, suggest equalities and inequalities related with center of inscribed sphere, and prove theses using the center of mass. Our results can be used in research and education programs, various types of gifted student education.

• Communications of Mathematical Education
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• v.25 no.2
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• pp.381-402
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• 2011

In this paper we study formulae of the triangle's area. We solve problems related with making new formulae of the triangle's area. These formulae is consisted of some elements of triangle, for example side, angle, median, perimeter, radius of circumcircle. We transform formulae $S=\frac{1}{2}acsinB$, $S=\frac{abc}{4R}$, $S=\sqrt{p(p-a)(p-b)(p-c)}$, and make new formulae of the triangle's area. Some formulas are received in the process of Research and Education program in the science high school. We expect that our results will be used in the Research and Education program in the science high school.

• Journal of Korea Society of Industrial Information Systems
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• v.11 no.2
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• pp.18-28
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• 2006

We consider a Direct Input Output Manufacturing System(DIOMS) which has a munber of machine centers placed along a built-in Automated Storage/Retrieval System(AS/RS). The Storage/Retrieval (S/R) machine handles parts placed on pallets for the operational aspect of DIOMS and determines the optimal operating policy by combining computer simulation and genetic algorithm. The operational problem includes: input sequencing control, dispatching rule of the S/R machine, machine center-based part type selection rule, and storage assignment policy. For each operating policy, several different policies are considered based on the known research results. In this paper, using the computer simulation and genetic algorithm we suggest a method which gives the optimal configuration of operating policies within reasonable computation time.

• International Journal of Computer Science & Network Security
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• v.22 no.2
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• pp.241-249
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• 2022

The current research attempts to measure the level of the acceptance of the Blackboard System (BBS) during the Corona crisis, and whether this is one of the reasons for the low use of the BBS at Al-Jouf University. To achieve this, the technology accepting model in the time of crisis (TAMTC) has been proposed to measure the degree of acceptance by students, which was then applied to a random sample of 339 of such. The results show a high level of student acceptance, despite their lower use of the system. The research also highlights the importance of upgrading e-courses and that the discontinuation of exam disqualification of students is secondary to their poor course attendance.

• Atmosphere
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• v.16 no.4
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• pp.379-385
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• 2006

Meteorological Research Institute (METRI) participates the R&E (Research and Education) program of Korea Science and Engineering Foundation,"Variability of ocean status around Ulleung basin and Dok-do by using ARGO data" as a part of "Carricula development for gifted students" program. From this program, we support students to have an opportunity for handling scientific data with advanced technology and inspire their scientific interests. In this article, we introduce the training processes of this program and the results of data analysis by the students themselves.

• Journal of Fisheries and Marine Sciences Education
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• v.28 no.5
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• pp.1258-1265
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• 2016

The aim of this study was to evaluate the impact on the fishery resources and marine ecosystems by derelict fishing gears as part of the R & E (Research and Education) program that supervised by the busan metropolitan city office of education. In this study, we verified the ghost fishing effects caused by derelict fishing gears through the process which fish were caught to be attracted to bait for gillnet and trap in tank. In results, the dead fish to be gilled in gillnet and to enter in pot have been shown to attract other fish to the bait role. The result showed that the ghost fishing was caused by abandoned, lost or discarded fishing gears continuously. In particular, as a result of pot experiment, if the lost pot has a escape device, the entered fish could escape from the pot and the fisheries resources could protect from derelict fishing gears. In addition, the high school students are capable of raising the problem solving skills through carrying out the research project and expect to contribute to raising awareness about the protection of the marine environment.

• Korean Journal of Environment and Ecology
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• v.27 no.5
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• pp.550-560
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• 2013

This study was investigated the morphological characters, secondary sexual dimorphism, and habitat patterns of Korean Rattus animals collected on Jeju Island from April 2005 to October 2012. Two wild rat species, namely R. norvegicus and R. tanezumi were identified on Jeju Island based on morphological characteristics and molecular data; however, R. rattus, which had recorded formerly, was not found in this study. Individuals of R. norvegicus were captured from urban, rural, and natural habitats, while those of R. tanezumi were specially found in animal farms and the surrounding areas. Comparing of morphological characters of two species, R. norvegicus had a shorter tail and ears than R. tanezumi (p<0.05), and the ratios of tail length and ear length to those of head-body length showed significantly differences between two species (p<0.05). The body weights (BW) of urban populations of R. norvegicus were significantly heavier than those of rural populations (p<0.05). No secondary sexual dimorphism was found in R. norvegicus, but females of R. tanezumi showed heavier BW than those of males (p<0.05). These findings suggest that it is necessary to revise the records for the existence of R. tanezumi and to confirm the animal fauna and elucidate the distribution and ecological characteristics from further studies using extensive sampling and detailed investigations on Jeju Island and also on the Korean Peninsula.

• The Journal of Korean Association of Computer Education
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• v.20 no.1
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• pp.75-86
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• 2017

E-learning has become one of the popular educational method in these day. In recognition of the growing e-learning, numerous researchers of S&T have utilized for the training aimed at enhancing competency. In the circumstances, previous studies have yield interesting results regarding certain factors that competency based programs may increase effectiveness of education. Therefore this research described here contributes to design competency modeling and education roadmap for human resource in S&T. The study uses survey, FGI, delphi technique, and expert workshop for selecting the main competencies. In particular, the results are including training roadmap of 5 level in each of two groups as researchers and S&T managers. These findings can be possible to develop customized programs and supported long-term career development path plan for human resource in S&T.