• Asian Pacific Journal of Cancer Prevention
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• 제13권4호
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• pp.1557-1561
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• 2012

Objective: To investigate the promoter methylation status of the E-cadherin gene in non-small cell lung cancer (NSCLC) and its association with clinical pathological parameters, and to explore the relationship between downregulation of E-cadherin gene expression and the methylation status of its promoter region. Methods: Nested methylation-specific PCR was performed to examine CpG methylation within the 5' CpG island of the E-cadherin gene in lung cancer and para-cancerous tissue from 37 patients with primary non-small cell lung cancer. Quantitative real-time PCR was performed to measure the level of E-cadherin mRNA. Results: Of thirty-seven cases, 12 (32.4%) samples showed aberrant CpG methylation in tumor tissues compared with the corresponding normal tissues. In addition, a reduction in E-cadherin mRNA levels was observed in 11 of the 12 (91.7%) tumor tissues carrying a methylated E-cadherin gene. However, only 10 (43.5%) cases displayed reduced mRNA levels in tumor tissues from the remaining 23 cases (excluding 2 samples from which mRNA was unavailable) without methylation events. Downregulation of E-cadherin gene expression significantly correlated with the promoter methylation status of this gene. Conclusion: These results provide strong evidence that the methylation status of E-cadherin gene contributes to a reduction in the expression of E-cadherin mRNA, and may play a role in the development and progression of NSCLC.

• Asian Pacific Journal of Cancer Prevention
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• 제15권4호
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• pp.1685-1688
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• 2014

Background: CEA and CA 15.3 serum tumor markers are currently used in clinical practice for monitoring therapy. The aim of this study was to evaluate serum level of these markers among healthy females and invasive breast carcinoma (IBC) patients and to determine any relationships with clinicopathological factors. Materials and Methods: 60 Iranian females were enrolled in this study, 30 healthy and 30 diagnosed with breast cancer who had not received any preoperative chemotherapy or hormone therapy. Enzyme linked immunosorbent assays were used for the quantitative determination of the cancer associated antigens, CEA and MUC1 (CA15-3). Results: The serological levels of CEA and CA15-3 ($5.0033{\pm}0.49{\mu}g/L$ and $178.1667{\pm}15.11$ U/ml) in the breast cancer patients were significantly higher (p=0.00) than the serum levels of normal controls ($1.1237{\pm}0.11{\mu}g/L$ and $21.13{\pm}3.058$ U/ml). Regarding the CEA marker, a significant correlation with grade of tumor was shown. Furthermore, there was a low correlation between CA15-3 and CEA marker with correlation coefficient r=0.08. Conclusions: Collectively, markedly high levels of CEA and CA15-3 were found in our patients, pointing to their use as additional tools after clinical diagnosis.

• 제어로봇시스템학회논문지
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• 제21권5호
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• pp.421-426
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• 2015

Need to develop human body's posture supervised robots, gave the push to researchers to think over dexterous design of exoskeleton robots. It requires to develop quantitative techniques to assess human motor function and generate the command to assist in compliance with complex human motion. Upper limb rehabilitation robots, are one of those robots. These robots are used for the rehabilitation of patients having movement disorder due to spinal or brain injuries. One aspect that must be fulfilled by these robots, is to cope with uncertainties due to different patients, without significantly degrading the performance. In this paper, we propose chattering free sliding mode control technique for this purpose. This control technique is not only able to handle matched uncertainties due to different patients but also for unmatched as well. Using this technique, patients feel active assistance as they deviate from the desired trajectory. Proposed methodology is implemented on seven degrees of freedom (DOF) upper limb rehabilitation robot. In this robot, shoulder and elbow joints are powered by electric motors while rest of the joints are kept passive. Due to these active joints, robot is able to move in sagittal plane only while abduction and adduction motion in shoulder joint is kept passive. Exoskeleton performance is evaluated experimentally by a neurologically intact subjects while varying the mass properties. Results show effectiveness of proposed control methodology for the given scenario even having 20 % uncertain parameters in system modeling.

• Bulletin of the Korean Chemical Society
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• 제35권12호
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• pp.3637-3641
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• 2014

In this study, we analyzed the toxicity of mixtures of dimethylformamide (DMF) and methyl ethyl ketone (MEK) or DMF and toluene (TOL) and predicted their toxicity using quantitative structure-activity relationships (QSAR). A QSAR model for single substances and mixtures was analyzed using multiple linear regression (MLR) by taking into account the statistical parameters between the observed and predicted $EC_{50}$. After preprocessing, the best subsets of descriptors in the learning methods were determined using a 5-fold cross-validation method. Significant differences in physico-chemical properties such as boiling point (BP), specific gravity (SG), Reid vapor pressure (rVP), flash point (FP), low explosion limit (LEL), and octanol/water partition coefficient (Pow) were observed between the single substances and the mixtures. The $EC_{50}$ of the mixture of DMF and TOL was significantly lower than that of DMF. The mixture toxicity was directly related to the mixing ratio of TOL and MEK (MLR $EC_{50}$ equation = $1.76997-1.12249{\times}TOL+1.21045{\times}MEK$), as well as to SG, VP, and LEL (MLR equation $EC_{50}=15.44388-19.84549{\times}SG+0.05091{\times}VP+1.85846{\times}LEL$). These results show that QSAR-based models can be used to quantitatively predict the toxicity of mixtures used in manufacturing industries.

• 대한화장품학회지
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• 제30권1호
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• pp.135-140
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• 2004

피부 노화를 예방할 수 있는 기능성 화장품은 지질과산화를 초래하는 활성산소종(ROS)의 생성 억제와 제거 또는 collagen과 elastin의 사슬 절단 및 교차결합의 변형을 억제하는 효능이 있어야 한다. 본 연구에서는 피부 조직 중 malonedialdehydt (MDA)와 collagen이 항노화 작용이 있는 기능성 화장품의 검색 지표로서의 사용 가능성을 알아보고자 10, 25 주령 랫드의 정상 피부와 창상을 유도한 피부를 7일간 관찰하였다. 육안적인 관찰에서는 10 주령군의 11마리 랫드 중 10마리에서, 노령 랫드군의 11마리 중 8마리에서 창상이 폐쇄되었다. 10 주령 랫드와 비교했을 때 노령 랫드에서 흉터의 길이는 긴 반면에 외피의 폐쇄, collagen 밀도, 외피의 두께, 총 hydroxyproline (hyp)과 MDA 농도가 유의적으로 낮게 나타났다( p〈0.05 ∼ p 〈 0.005). 10 주령에서 진피의 규칙적으로 배열된 collagen 섬유다발과 많은 섬유아세포의 관찰과는 반대로 25 주령 이상 랫드에서는 collagen 섬유다발 사이에 많은 낭포, 소와 및 섬유아세포 수의 감소, 느슨한 외피와 진피의 결합과 얇은 외피가 관찰됨으로서 25 주령 이상의 랫드에서 감소된 hyp 양은 감소된 collagen 밀도 및 형태학적 변화와 일치하였다. 따라서 collagen 합성과 축적의 지표인 hyp의 측정은 항 피부노화 예방 및 개선제의 모니터링 연구에서 매우 유용하며 또한 피부 노화의 검객 지표로 사용이 가능하다고 사료된다.

• International Journal of Industrial Entomology and Biomaterials
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• 제7권2호
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• pp.221-229
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• 2003

Ten multivoltine female parental lines were crossed with popular bivoltine male silkworm breed NB$_4$D$_2$. Three types of heterosis parameters viz., heterosis over mid-parental value (hybrid vigour), heterobeltiosis (useful heterosis) and standard heterosis (standard check) were estimated for 15 economically important quantitative traits. The interaction among the hybrids and parents indicated significant effect for maximum characters. The heterotic effect of new hybrid combination was compared with popular hybrids viz., Pure Mysore${\times}$NB$_4$D$_2$ and Nistari${\times}$NB$_4$D$_2$. Varied heterotic effect was observed for different traits for different hybrid combination. The results inferred that the crosses viz., BL$_{23}$${\times}$NB$_4$D$_2$ ranked top for 14 traits followed by Hosa Mysore${\times}$NB$_4$D$_2$ for 11 traits; PA$_{12}$${\times}$NB$_4$D$_2$ for 9 traits; BL$_{24}$${\times}$NB$_4$D$_2$ for 8 traits; Kolar Gold${\times}$NB$_4$D$_2$ for 7 traits; WAI$_1$${\times}$NB$_4$D$_2$ for 6 traits and MU$_{11}$${\times}$NB$_4$D$_2$ for 5 traits. Among these, the best hybrids Kolar Gold${\times}$NB$_4$D$_2$ and MU$_{11}$${\times}$NB$_4$D$_2$ were identified for longer filament length and fine denier. Similarly for higher cocoon yield and silk productivity BL$_{223}$${\times}$NB$_4$D$_2$ and BL$_{24}$${\times}$NB$_4$D$_2$ were found to be superior. These hybrid combinations are suitable for commercial exploitation at large scale.e.e.e.

• 한국지반환경공학회 논문집
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• 제10권7호
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• pp.81-91
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• 2009

근래에 들어 터널계획시 방재 및 환경훼손 측면을 고려하여 상, 하행터널이 나란히 배열되는 병설터널의 형태로 계획되는 사례가 증가하고 있으나, 이에 대한 연구성과는 상대적으로 미미한 실정이다. 병설터널 사이에 위치하는 필라부는 연직응력의 증가와 수평응력의 감소에 의해 병설터널 주변암반의 불안정성이 증가하며, 이와 같은 불안정성은 필라폭이 감소할수록 더욱 증가하게 된다. 본 연구에서는 병설터널의 굴진에 따른 필라부의 간섭효과와 응력변화거동의 분석을 위한 다양한 조건들에 대해 수치해석을 시행하였으며, 수치해석 결과에 대한 회귀분석을 통해 필라의 유발응력평가식을 제안하였다. 또한, 제안식과 Hoek-Brown 파괴기준을 적용하여 필라부의 정량적인 안전율 산정기법을 제안하였고, 제안된 기법의 적용성 확대를 위해 보강공법이 적용된 경우를 고려할 수 있는 해석기법을 아울러 제시하였다. 마지막으로 다양한 변수 분석을 통해 Hoek-Brown 파괴기준을 기초로 제안된 본 기법의 효율성을 입증하였다.

• Steel and Composite Structures
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• 제16권6호
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• pp.703-718
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• 2014

The initial clamping forces of high strength bolts subjected to different faying surface conditions drop within 500 hours regardless of loading, any other external force or loosening of the nut. This study develops a mathematical model for relaxation confined to creep on a coated faying surface after initial clamping. The quantitative model for estimating relaxation was derived from a regression analysis for the relation between the creep strain of the coated surface and the elapsed time for 744 hours. This study establishes an expected model for estimating the relaxation of bolted joints with diverse coated surfaces. The candidate bolts are dacro-coated tension control bolts, ASTM A490 bolt, and plain tension control bolts. The test parameters were coating thickness, species of coating. As for 96, 128, 168, and $226{\mu}m$ thick inorganic zinc, when the coating thickness was increased, relaxation after the initial clamping rose to a much higher range from 10% to 18% due to creep of the coating. The amount of relaxation up to 7 days exceeded 85% of the entire relaxation. From this result, the equation for creep strain can be derived from a statistical regression analysis. Based on the acquired creep behavior, it is expected that the clamping force reflecting relaxation after the elapse of constant time can be calculated from the initial clamping force. The manufacturer's recommendation of inorganic zinc on faying surface as $75{\mu}m$, appears to be reasonable.

• 한국터널지하공간학회 논문집
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• 제6권2호
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• pp.113-128
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• 2004

본 연구에서는 아치형 복개 터널구조물에 작용하는 토압에 영향을 미치는 주요 영향인자들을 평가하기 위하여, 지반정수 및 굴착과 관련된 영향인자들에 대하여 몬테카를로 시뮬레이션 기법에 근거한 정량적 민감도 분석을 수행하였으며, 민감도 분석을 토대로 최대 영향인자 변화에 따른 토압의 변화에 대한 수치해석적 검토를 수행하였다. 민감도분석에 사용되는 출력결과들은 유한요소해석에서 얻어진 터널 위치별 연직 및 수평토압을 대상으로 하였다. 민감도 분석결과, 아치형 복개 터널구조물에 작용하는 수평 및 연직토압은 작용위치에 따라 동일한 영향인자에 대한 민감도가 상이한 것으로 나타났으며, 또한 최대 영향인자인 토피고 및 굴착사면 변화에 따라 아치형 복개 터널구조물에 작용하는 토압분포 및 크기가 변화하는 것으로 나타났다.

• Asian-Australasian Journal of Animal Sciences
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• 제24권2호
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• pp.162-172
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• 2011

In this paper, simulation was used to determine accuracies of genomic breeding values for polygenic traits associated with many thousands of markers obtained from high density genome scans. The statistical approach was based upon stochastically simulating a pedigree with a specified base population and a specified set of population parameters including the effective and noneffective marker distances and generation time. For this population, marker and quantitative trait locus (QTL) genotypes were generated using either a single linkage group or multiple linkage group model. Single nucleotide polymorphism (SNP) was simulated for an entire bovine genome (except for the sex chromosome, n = 29) including linkage and recombination. Individuals drawn from the simulated population with specified marker and QTL genotypes were randomly mated to establish appropriate levels of linkage disequilibrium for ten generations. Phenotype and genomic SNP data sets were obtained from individuals starting after two generations. Genetic prediction was accomplished by statistically modeling the genomic relationship matrix and standard BLUP methods. The effect of the number of linkage groups was also investigated to determine its influence on the accuracy of breeding values for genomic selection. When using high density scan data (0.08 cM marker distance), accuracies of breeding values on juveniles were obtained of 0.60 and 0.82, for a low heritable trait (0.10) and high heritable trait (0.50), respectively, in the single linkage group model. Estimates of 0.38 and 0.60 were obtained for the same cases in the multiple linkage group models. Unexpectedly, use of BLUP regression methods across many chromosomes was found to give rise to reduced accuracy in breeding value determination. The reasons for this remain a target for further research, but the role of Mendelian sampling may play a fundamental role in producing this effect.