Journal of the Society of Cosmetic Scientists of Korea (대한화장품학회지)

Society of Cosmetic Scientists of Korea (대한화장품학회)
권호 표지

Quantitative Changes of Collagen and Malonedialdehyde as the Parameters of Skin Alteration

피부노화의 지표가 되는 collagen과 malonedialdehyde의 정량적인 변화

  • 김기영 (원광대학교 의과대학 병리학교실) ;
  • 이재형 (원광보건대학 물리치료학) ;
  • 진주영 (원광대학교 한의학전문대학) ;
  • 양시용 (원광대학교 한의학전문대학원)
  • Published : 2004.05.01
Download PDF
⟨ Previous Next ⟩

Abstract

Anti-skin aging agent could be have an inhibition effect of ROS production as well as fragmentation and change of collagen cross linkage in collagen molecule. For the monitoring of lipid peroxidation and collagen degradation, the skin of young and old rats were incised and observed 7 days. In the result, the wound closure was observed in the skin from 10 of 11 young rats and in 8 of 11 old rats. And the longer wound length but shorter wound closure, weaker collagen density and thicker epidermis were observed in old rats than in young rats. The level of hydroxyproline as a parameter of collagen synthesis and MDA as a parameter of lipid peroxidation was lower in old group than in young group. The cyst and lacuna between collagen bundle and fibroblast were observed in old rats in contrast to young rats. So that we propose that MDA and hydroxyproline could be used for monitoring of anti-skin aging agent.

피부 노화를 예방할 수 있는 기능성 화장품은 지질과산화를 초래하는 활성산소종(ROS)의 생성 억제와 제거 또는 collagen과 elastin의 사슬 절단 및 교차결합의 변형을 억제하는 효능이 있어야 한다. 본 연구에서는 피부 조직 중 malonedialdehydt (MDA)와 collagen이 항노화 작용이 있는 기능성 화장품의 검색 지표로서의 사용 가능성을 알아보고자 10, 25 주령 랫드의 정상 피부와 창상을 유도한 피부를 7일간 관찰하였다. 육안적인 관찰에서는 10 주령군의 11마리 랫드 중 10마리에서, 노령 랫드군의 11마리 중 8마리에서 창상이 폐쇄되었다. 10 주령 랫드와 비교했을 때 노령 랫드에서 흉터의 길이는 긴 반면에 외피의 폐쇄, collagen 밀도, 외피의 두께, 총 hydroxyproline (hyp)과 MDA 농도가 유의적으로 낮게 나타났다( p〈0.05 ∼ p 〈 0.005). 10 주령에서 진피의 규칙적으로 배열된 collagen 섬유다발과 많은 섬유아세포의 관찰과는 반대로 25 주령 이상 랫드에서는 collagen 섬유다발 사이에 많은 낭포, 소와 및 섬유아세포 수의 감소, 느슨한 외피와 진피의 결합과 얇은 외피가 관찰됨으로서 25 주령 이상의 랫드에서 감소된 hyp 양은 감소된 collagen 밀도 및 형태학적 변화와 일치하였다. 따라서 collagen 합성과 축적의 지표인 hyp의 측정은 항 피부노화 예방 및 개선제의 모니터링 연구에서 매우 유용하며 또한 피부 노화의 검객 지표로 사용이 가능하다고 사료된다.

Keywords

References

  1. J. Soc. Cos. Sci. Kor. v.23 Anti-aging in cosmentics H.H.Kang
  2. Zhongguo Zhong Yao Za Zhi v.28 Effects of the extract from bergarnot and boxthorn on the delay of skin aging and hair growth in mice L.X.Shao
  3. J. Soc. Cos. Sci. Kor. v.25 Changes of facial wrinkle after topical application of on emulsion containing medimin A S.K.Park;M.Y.Chang;Y.D.Kim;Y.D.Kim;B,Y.Jeong;Y.H.Won;J.J.Kim;S.H.Kang
  4. J. Soc. Cos. Sci. Kor. v.26 Isolation and characterization of elastase inhibitor from Areca catechu J.J.Cho;G.K.Lee;B.K.Cho;J.D.Choi
  5. J. Soc. Cos. Sci. Kor. v.23 The effects of retinoids on Crabp Ⅱ mRNA induction and collagen synthesis in human dermal fibroblast J.S.Hwang
  6. J. Soc. Cos. Sci. Kor. v.24 Effect of Korean black soybean seed on the cellular proliferation and the production of type Ⅲ collagen in skin fibroblast C.W.Lee;H.B.Pyo;Y.H.Cho;S.M.Park
  7. J. Soc. Cos. Sci. Kor. v.10 A promising new anti-wrinkle ingredient: Pericarpium castaneae extracts B.J.Kim;B.K.Jo;J.H.Kim
  8. Arch. Biochem. Biophys. v.288 Peroxynitrite-induced membrane lipid peroxidation; The cytotoxic potential of superoxide and nitric oxide R.Radi;J.S.Beckman;K.M.Bush;B.A.Freeman https://doi.org/10.1016/0003-9861(91)90224-7
  9. Lab. Invest. v.69 Cell and tissue responses to oxidative damage Y.M.Janssen;B.Van Houten;P.J.Borm;BT.Mossman
  10. J. Soc. Cos. Sci. Kor. v.18 The role of reactive oxygen species on UVA-induced aging of dermal collagen S.J.Kang
  11. Fitoterapia v.74 Comparative effect of oral administration and topical application of alcoholic extract of Terminalia arjuna bark on incision and excision wounds in rats M.M.Rane;S.A.Mengi https://doi.org/10.1016/S0367-326X(03)00118-7
  12. Hepatol. v.11 Collagen typeⅠ and Ⅲ occur together in hybrid in the space of disse the rat liver A.Geerts;D.Schuppan;S.Lazeroms;R.De Zanger;E.Wisse
  13. Mesenchymal-epithelial interactions in neural development, Nato Asi. v.5 Components of the etracellular matrix (collagen, elastin, glycoproteins and proteoglycans) D.Schuppan;E.Hahn;J.R.Wolff(et al.)
  14. Phytother Res. v.17 Pro-healing effect of Cinnamomum zeylanicum bark J.V.Kamath;A.C.Rana;A.R.Chosdhury https://doi.org/10.1002/ptr.1293
  15. Br. J. Dermatol. v.149 Protective and determining factors for the overall lipid peroxidation in ultraviolet Al-irradaiated fibroblasts: in vitro and in vivo investigations J.Dissmond;L.A.Schneider;P.Brenneisen;K.Briviba;J.Wenk;M.Wlaschek;K.Scharffetter-Kochanek https://doi.org/10.1046/j.1365-2133.2003.05457.x
  16. Anal. Biochem. v.112 A Single method to determine nanogram levels of 4-thdroxyproline in biological tissue I.S.Jamall;V.N.Finelli https://doi.org/10.1016/0003-2697(81)90261-X
  17. Anal. Biochem. v.95 Assay for lipid peroxides in animal tissues by thiobarbituric acid reaction H.Ohkawa;N.Ohishi;K.Yagi https://doi.org/10.1016/0003-2697(79)90738-3
  18. J. Soc. Cos. Sci. Kor. v.7 Skin aging J.I.Yun
  19. J. Dermatological Science v.27 Eqidermal changes caused by chronic low-dose UV irradiation induce wrinkle formation in hariless mouse H.Kambayashi;M.Yamashita;Y.Odake;K.Takada;Y.Funasaka;M.Ichiashi https://doi.org/10.1016/S0923-1811(01)00113-X
  20. Wound Repair Regen v.12 Dermal fibroblasts from different layers of human skin are heterogeneous in expression of collagenase and typesⅠ and Ⅲ procollagen mRNA M.Ali Bahar;B.Bauer;E.Tredget;A.Ghahary https://doi.org/10.1111/j.1067-1927.2004.012110.x
  21. Photodermatol v.6 A biochemical and immunohis-tochemical study of collagen in sun-exposed and pro-tected skin A.Oikarnin;M.Kallionen