• 생약학회지
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• 제49권1호
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• pp.70-75
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• 2018

Acer tegmentosum Maxim (ATM) has been used to treat hepatic disorders in traditional oriental medicine. However, there is little information about phytochemical constituents for quality control of ATM. In this study, we developed and established a simultaneous analytical method of the 10 marker compounds (three coumarins, 3 flavonoids, 1 lignan, 3 phenolics) in ATM using ultra-performance liquid chromatography-mass spectrometry (UPLC-MS/MS). Chromatographic separation of ten target analytes was achieved with a Waters Acquity UPLC BEH $C_{18}$ analytical column ($2.1{\times}100mm$, $1.7{\mu}m$), using a mobile phase of 0.1% (v/v) formic acid in water and acetonitrile with gradient elution. Identifications and quantitation of all analytes were performed using a Q-Exactive UPLC-MS/MS system. Correlation coefficients of the calibration curve for all analytes were ${\geq}0.9986$. The values of limits of detection and quantification of all analytes were 0.5-10.0 and 5.0-50.0 ng/mL, respectively. The established UPLC-MS/MS method successfully identified all target analytes in ATM, and the phytochemicals were 0.01-67.98 mg/g in its lyophilized water extract.

• Mass Spectrometry Letters
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• 제2권2호
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• pp.37-40
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• 2011

Saccharin is a commonly used artificial sweetener in foodstuffs. However, for its carcinogenic dispute, it has been regulated by government bodies. In this study, isotope dilution mass spectrometry (ID-MS) was introduced for the accurate quantification of saccharin. To employ ID-LC/MS, we obtained its isotope analogue, $^{13}C_1$-sodium saccharin, by customized synthesis. Samples were spiked with $^{13}C_1$-sodium saccharin and analyzed with LC/MS in negative mode. Chromatographic conditions were optimized for the adequate chromatographic retention and separation of saccharin with a $C_{18}$ column. MS was operated with electrospray ionization by the selected ion monitoring (SIM) mode of $[M-H]^-$ for saccharin (m/z 182) and $[M-Na]^-$ for its isotope analogue (m/z 183). To validate the ID-LC/MS method for accurate measurement, we prepared a batch of a candidate material by sortifying quasi-tea-drinks with saccharin and analyzed samples gravimetrically fortified in various levels of concentration. The repeatability and reproducibility of this method was tested by analyzing the reference material. Result show that ID-LC/MS is a reliable method for the quantitative analysis of saccharin.

• 한국감성과학회:학술대회논문집
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• 한국감성과학회 2008년도 추계학술대회
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• pp.46-49
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• 2008

This study aims to suggest basic materials to develop an emotional font for future Hangeul font development plan by investigating emotional reaction displayed through the subjects and by quantifying its contribution of the stimulation elements causing such reaction. For this, a survey of 150 university students who are currently enrolled in the College of Design was conducted in order to analyze correlation between 30 emotional words extracted from the Hangeul font and the selected 36 sample fonts. The combined data was described with the two dimensional dispersion using the quantification theory type III. The analysis found out that the Hangeul font forms the x-axis showing "soft(dynamic).hard(static)" and the y-axis showing "modern(light).classic(heavy)”. Specifically, there exist emotional groups such as "archaic","masculine","feminine","negative", and "modern static"on each axis. In addition, to extract the casual relationship between the value of emotional reaction and its stimulation elements quantitatively, the author indicated the emotional words of each axis and the total value of equivalent five emotional word groups as the standard variance and the constituent of Hangeul font as the independent variable, and then the quantification theory type I was used to analyze the physical elements of the Hangeul font. As a result, "the centerline of gravity(base line)" and "thickness variance of a stroke" on the two axes, and "the centerline of gravity(base line)" and "decoration" among the five groups were identified as the most influential elements that affect the emotional reaction of the subjects.

• Journal of Microbiology and Biotechnology
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• 제22권2호
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• pp.248-255
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• 2012

In order to develop a protocol to quantify cyanobacteria and Microcystis simultaneously, the primers and probe were designed from the conserved regions of 16S rRNA gene sequences of cyanobacteria and Microcystis, respectively. Probe match analysis of the Ribosomal Database Project showed that the primers matched with over 97% of cyanobacterial 16S rRNA genes, indicating these can be used to amplify cyanobacteria specifically. The TaqMan probe, which is located between two primers, matched with 98.2% of sequences in genus GpXI, in which most Microcystis strains are included. The numbers of cyanobacterial genes were estimated with the emission of SYBR Green from the amplicons with two primers, whereas those of Microcystis spp. were measured from the fluorescence of CAL Fluor Gold 540 emitted by exonuclease activity of Taq DNA polymerase in amplification. It is expected that this method enhances the accuracy and reduces the time to count cyanobacteria and potential toxigenic Microcystis spp. in aquatic environmental samples.

• 한국식품과학회지
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• 제53권6호
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• pp.682-687
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• 2021

The purpose of this study was to validate a high-performance liquid chromatography (HPLC) method for the quantitative analysis of quercetin in various foods. The method was based on HPLC-UV (360 nm). The method was validated using candy, beverage, and sausage which were tested for specificity, linearity, limit of detection (LOD), limit of quantification (LOQ), precision, and accuracy, and the measurement uncertainty was assessed. Matrix-matched calibration was also applied. The calibration curves (0.5-50 mg/L) showed good linearity (r²≥0.9998). LOD and LOQ ranged from 0.15 to 0.31 mg/kg and from 0.44 to 0.93 mg/kg, respectively. The average accuracy and precision at 0.5, 2.5, and 10 mg/kg ranged from 84.3 to 102.0% and 0.7 to 3.0 relative standard deviation (RSD%), respectively. This study confirmed the applicability of the proposed method by applying it to commercial products, such as teas and beverages. Thus, the proposed analytical method is suitable for quantifying quercetin in various foods.

• 교육녹색환경연구
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• 제18권2호
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• pp.1-11
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• 2019

본 연구는 지속적으로 증가가 예상되는 폐교시설의 효율적인 활용을 위하여 활용지표의 개발을 통해 폐교 활용 시 객관적인 의사결정을 위한 목적을 가진다. 연구 단계는 크게 폐교 활용 예비지표 도출, 빅데이터를 활용한 최종지표 도출, 지표의 정량화 단계로 구분하여 진행하였으며, 최종적으로 지표를 정량화함으로써 객관화하였다. 향후 지표를 기준으로 시설에 적용 및 검증하고자 한다. 본 연구는 지금까지 폐교시설의 활용을 위한 계획 및 연구에 있어서 시도되지 않았던 빅데이터 분석기법을 적용한 것에 그 의의가 있다.

• 분석과학
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• 제35권5호
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• pp.218-227
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• 2022

A simple and fast throughput flow injection (FI) system with a merging-zone technique was designed to determine ferrous and ferric in an aqueous solution. The method is based on the direct reaction of ferrous with a Bathophenanthroline reagent (Bphen) in acidic media. The forming red complex absorbs light at 533 nm. All conditions of the flow injection system were investigated. The analytical curve of ferrous was linear in the range of 0.07 to 4 mg/L with an r² value of 0.9968. The detection and quantification limits were 0.02 and 0.04 mg/L, respectively. The molar absorptivity and Sandell's sensitivity were 4.0577 × 10⁶ L/mol cm and 25 × 10^-5 ㎍/cm², respectively. The homemade valve was low-cost with high repeatability (n = 7) at an RSD of 1.26 % and zero dead volume. The values of the dispersion coefficient were 2.318, 2.022, and 1.636 for the concentrations of 0.2, 1, and 3 mg/L, respectively. The analysis throughput of the designed flow injection unit was 57 sample per hour.

• 분석과학
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• 제34권4호
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• pp.153-159
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• 2021

In this study, we describe the development of a new high-performance liquid chromatography (HPLC) method for the simultaneous analysis of six bioactive compounds (including gallic acid, epicatechin 3-gallate, quercitrin, afzelin, quercetin, and kaempferol) from Orostachys japonicus. The extraction method was investigated and optimization of the extraction time (min), solvent composition (%), and solvent to material ratio were conducted. As a result, 30 min extraction with 50% methanol and 40:1 mL/g of solvent: material ratio achieved the highest extraction efficiency with a yield of 3.32 mg/g. Furthermore, the developed HPLC method was validated and the correlation coefficient (R) values were within the satisfactory range of 0.9995-0.9999 over the linearity range of 1.53-417 ㎍/mL. The limit of detection and limit of quantification for the six active components were between 0.03-0.08 ㎍/mL and 0.08-0.26 ㎍/mL, respectively. With these newly optimized and developed methods, four batches of O. japonicus were analyzed to confirm the high extraction efficiency of the method and the feasibility of an application.

• Nuclear Engineering and Technology
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• 제55권1호
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• pp.119-130
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• 2023

Among the various elements of probabilistic safety assessment (PSA), human failure events (HFEs) and their dependencies are major contributors to the quantification of risk of a nuclear power plant. Currently, the dependency among HFEs is reflected using a post-processing method in PSA, wherein several drawbacks, such as limited propagation of minimal cutsets through the fault tree and improper truncation of minimal cutsets exist. In this paper, we propose a method to model the HFE dependency directly in a fault tree using the if-then-else logic. The proposed method proved to be equivalent to the conventional post-processing method while addressing the drawbacks of the latter. We also developed a software tool to facilitate the implementation of the proposed method considering the need for modeling the dependency between multiple HFEs. We applied the proposed method to a specific case to demonstrate the drawbacks of the conventional post-processing method and the advantages of the proposed method. When applied appropriately under specific conditions, the direct fault-tree modeling of HFE dependency enhances the accuracy of the risk quantification and facilitates the analysis of minimal cutsets.

• 대한안전경영과학회:학술대회논문집
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• 대한안전경영과학회 2005년도 춘계학술대회
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• pp.279-290
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• 2005

The purpose of this study is to attempt a Analysis of a pointed-end Equipment Arm Safety-Accident for Fault Tree Analysis. Three major techniques were used first problem is Z-Model by which accident Analysis & prevention of a pointed-end Industry can be made, Fault Tree Analysis(FTA) bywhich quantification of a pointed-end Equipment accident Analysis can be made it 5 years in past and the third, manual-written by which minimal cut set to accident can be Identified. A example has been made of issue point a pointed-end Equipment that the Arm in loader happen to Injuries. According to the Analysis lack of safety knowledge, unsafety-behavior seem to be the primal cause of accident. Comparision of the accident cause to actual report demonstratesthat the FTA a efficient tool for Industrial Accident prevention.