• 한국미생물·생명공학회지
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• 제17권3호
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• pp.241-246
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• 1989

Quadrupole mass spectrometer를 이용한 발효 배기가스의 분석을 통해 세포의 증식을 on-line monitoring하고자 model 균주로 Candida utilis에 대해 연구하였다. Quadrupole mass spectrometer와 interface된 16-bit 개인용 컴퓨터 (IBM PC-AT)에서 산소 소비속도(OUR)와 이산화탄소 발생속도(CER)를 on-line 계산할 수 있었고 계속해서 이들 계산치로부터 세포농도와 증식속도 및 비증식속도를 계산하였다. 계산된 값들은 실험적으로 측정한 세포농도 및 비증식속도와 잘 일치함을 알 수 있었다.

• 대한기계학회논문집B
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• 제25권5호
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• pp.741-749
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• 2001

An inhomogeneous hyperbolic electric field is established among two-dimensional quadrupole electrodes to which an ac voltage is applied. Conditions under which charged particles are focused into a narrow axis region of the plug laminar flow are discussed. The aerodynamic forces influence the behavior of the charged particles in the quadrupole electric field. We derived the dimensionless equations of motion of a charged particle in the alternating quadrupole electric field, and discussed particle trajectories and focusing performance in terms of two dimensionless parameters, which are functions of particle size, operating pressure, and the amplitude and frequency of applied AC voltage, with the results of numerical simulations and experiments.

• 한국초전도ㆍ저온공학회논문지
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• 제18권4호
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• pp.35-39
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• 2016

In recent years, high-temperature superconductor (HTS) Quadruple Triplets are being developed for heavy ion accelerators, because the HTS magnets are suitable to withstand radiation and high heat loads in the hot cell of accelerators. Generally, an iron yoke, which costs a mass of material, was employed to enhance the magnetic field when a quadrupole magnet was designed. The type of the magnet is called iron-dominated magnet, because the total magnetic field was mainly induced by the iron. However, in the HTS superconductor iron-dominated magnets, the coil-induced field also can have a certain proportion. Therefore, the air-core HTS quadrupole magnets can be considered instead of the iron-core HTS quadrupole magnet to be employed to save the iron material. This study presents the design of an air-core HTS quadruple triplet which consists three by air-core HTS quadruple magnet and compare the design result with that of an iron-core HTS quadruple triplet. First, the characteristics of an air-core HTS quadrupole magnet were analyzed to select the magnet system for the magnetic field uniformity impairment. Then, the field uniformity was improved(< 0.1%) exactly using evolution strategy (ES) method for each iron-core HTS quadrupole magnet and the air-core HTS quadruple triplet was established. Finally, the designed air-core triplet was compared with the iron-core HTS quadruple triplet, and the results of beam trajectories were presented with both the HTS quadruple triplet systems to show that the air-core triplet can be employed instead of the iron-core HTS triplet. The design of the air-core quadruple triplet was suggested for a heavy ion accelerator.

• KSBB Journal
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• 제4권2호
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• pp.118-122
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• 1989

Candida utilis를 model균주로 하여 세포증식과 기질소모를 on-line 간접측정하고자 개인용 컴퓨터(IBM PC-AT)와 interface된 quadrupole mass spectrometer로 발효 배기가스를 분석하였다. 5분마다 연속적으로 분석되는 질소, 산소, 이산화 탄소 및 수증기의 mole백분율(%)로부터 산소 소비속도(OUR)와 이산화 탄소 발생속도(CER)를 on-line으로 계산하였다. 미리 실험 결과치로부터 결정한 maintenance와 수율 상수들($k_1k_2 $ 값을 이용하여 세포농도와 기질인 포도당의 농도를 on-line으로 간접추정하였다. 간접추정된 값들은 실험적으로 측정한 세포농도 및 기질농도와 잘 일치함을 알 수 있었다.

• Journal of Microbiology and Biotechnology
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• 제26권6호
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• pp.1124-1131
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• 2016

In this study, the global proteome of the IPEC-J2 cell line was evaluated using ultra-high performance liquid chromatography coupled to a quadrupole Q Exactive^™ Orbitrap mass spectrometer. Proteins were isolated from highly confluent IPEC-J2 cells in biological replicates and analyzed by label-free mass spectrometry prior to matching against a porcine genomic dataset. The results identified 1,517 proteins, accounting for 7.35% of all genes in the porcine genome. The highly abundant proteins detected, such as actin, annexin A2, and AHNAK nucleoprotein, are involved in structural integrity, signaling mechanisms, and cellular homeostasis. The high abundance of heat shock proteins indicated their significance in cellular defenses, barrier function, and gut homeostasis. Pathway analysis and annotation using the Kyoto Encyclopedia of Genes and Genomes database resulted in a putative protein network map of the regulation of immunological responses and structural integrity in the cell line. The comprehensive proteome analysis of IPEC-J2 cells provides fundamental insights into overall protein expression and pathway dynamics that might be useful in cell adhesion studies and immunological applications.

• Journal of Microbiology
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• 제45권6호
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• pp.547-552
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• 2007

Previously, we generated monoclonal antibodies (MAbs) that bound to the surface of human embryonic stem cells (hESCs) in an attempt to discover new hESC-specific surface markers. In this study, MAb 47-235 (IgG1, ${\kappa}$) was selected for further characterization. The MAb bound to the surface of undifferentiated hESCs but did not bind to mouse ESCs or mouse embryonic fibroblast cells in flow cytometric analysis. The antibody immunoprecipitated a 47 kDa protein from the lysates of cell surface-biotinylated hESCs. Identification of the protein by quadrupole time of flight tandem mass spectrometry revealed that 47-235 binds to Ag 243-5 protein of Mycoplasma arginini. BM-Cyclin treatment of the hESCs that reacted with 47-235 resulted in loss of mycoplasma DNA and the reactivity to 47-235. Nevertheless, the hESCs that were reactive to 47-235 maintained self-renewal and pluripotency and thus could be differentiated into three embryonic germ layers.

• Asian Pacific Journal of Cancer Prevention
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• 제15권13호
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• pp.5417-5421
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• 2014

Objectives: The identification of cutaneous T cell lymphoma (CTCL) biomarkers may serve as a predictor of disease progression and treatment response. The aim of this study was to map potential biomarkers in CTCL plasma. Design and Methods: Plasma metabolic perturbations between CTCL cases and healthy individuals were investigated using metabolomics and ultrahigh performance liquid chromatography-quadrupole time-of-flight mass spectrometry (UHPLC-QTOF/MS). Results: Principal component analysis (PCA) of the spectra showed clear metabolic changes between the two groups. Thirty six potential biomarkers associated with CTCL were found. Conclusions: Based on PCA, several biomarkers were determined and further identified by LC/MS/MS analysis. All of these could be potential early markers of CTCL. In addition, we established that heparin as a nticoagulant has better pre-treatment results than EDTA with the UHPLC-QTOF/MS appraoch.

• Mass Spectrometry Letters
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• 제11권2호
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• pp.36-40
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• 2020

Untargeted metabolomics is a useful tool for drug development focusing on novel chemotherapeutic and chemopreventative agents against cancer cells. In recent years, quadrupole time of flight liquid chromatography-mass spectrometry (Q-TOF LC/MS)-based untargeted metabolomic approaches have gained importance to evaluate the effect of these agents at the molecular level. The researchers working on cell culture studies still do not apply standardized methodologies on sample preparation for untargeted metabolomics approaches. In this study, the rough and wet lysis techniques performed on MCF-7 breast cancer cells were compared with each other via the Q-TOF LC/MS-based metabolomic approach. The C18 and hydrophilic interaction liquid chromatography (HILIC) columns were used for the separation of the metabolites in MCF-7 cell lysates. 505 peaks were detected through the HILIC column and 551 peaks were found through the C18 column for the wet lysis technique. This situation supported by the base peak chromatograms showed that the wet lysis technique allowed us to extract higher number of non-polar metabolites. Almost equal number of metabolites was found for the C18 and HILIC columns (697 peaks for the HILIC column and 695 peaks for the C18 column) when the rough lysis technique was used. However, the intensities of polar metabolites were higher for the rough lysis technique on base peak chromatograms for both the HILIC and C18 columns. Although cell lysis technique, which is the first step in the sample preparation for cell culture studies, does not cause dramatic differences in the number of the detected metabolite peaks, it affects the polar and non-polar metabolite ratio significantly. Therefore, it must be considered carefully especially for in vitro drug development studies.

• Journal of Microbiology and Biotechnology
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• 제27권6호
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• pp.1090-1097
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• 2017

Rhus verniciflua Stokes (RVS), an herbal medicine found in East Asia, was extracted and further fractionated to investigate its antioxidant capacity and neuroprotective effects. The RVS ethyl acetate (EtOAc) fraction had the highest level of total phenolics and antioxidant capacity among all solvent fractions tested. Pretreatment of PC-12 cells with the EtOAc fraction effectively attenuated $H_2O_2$-induced oxidative damage. Furthermore, the EtOAc fraction significantly attenuated caspase-3 activity, resulting in inhibition of $H_2O_2$-induced apoptosis. We identified and quantified fustin, sulfuretin, and butein in the EtOAc fraction using accurate mass quadrupole time-of-flight mass spectrometry and reversed-phase high-performance liquid chromatography. The intracellular antioxidant capacity and superoxide dismutase (SOD) activity were significantly increased in PC-12 cells treated with the EtOAc fraction and with individual flavonoids. When cells were pretreated with the EtOAc fraction or individual flavonoids and then co-incubated with diethyldithiocarbamic acid (an inhibitor of SOD activity), cell viability against $H_2O_2$-induced oxidative stress was attenuated. These results suggest that the RVS EtOAc fraction and its flavonoid constituents protect PC-12 cells against $H_2O_2$-induced neurotoxicity through their antioxidant properties.

• 대한약학회:학술대회논문집
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• 대한약학회 2003년도 Proceedings of the Convention of the Pharmaceutical Society of Korea Vol.1
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• pp.288.3-289
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• 2003

Sphingolipid species are important second messengers due to their role in the mitogenesis, differentiation and apoptosis. We developed a new column liquid chromatography-triple quadrupole tandem mass spectrometry (LC-MS/MS) in combination with multiple reaction monitoring (MRM) method for the rapid, simultaneous and quantitative determination of unambiguous detecting sphingolipids in cell culture of human cancer cells (HL-60). (omitted)