대한약학회:학술대회논문집 (Proceedings of the PSK Conference)
- 대한약학회 2003년도 Proceedings of the Convention of the Pharmaceutical Society of Korea Vol.1
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- Pages.288.3-289
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- 2003
Profiling Analysis of Sphingolipids in HL-60 Cells by High-Performance Liquid Chromatography-Tandem Mass Spectrometry in combination with Multiple Reaction Monitoring
- Son, Jung-Hyun (Bioanalysis and Biotransformation Research Center, Korea Institute of Science and Technology) ;
- Lee, Jae-Ick (Bioanalysis and Biotransformation Research Center, Korea Institute of Science and Technolog) ;
- Yang, Ryung (Department of Biotechnology, Yonsei University) ;
- Kim, Dong-Hyun (Bioanalysis and Biotransformation Research Center, Korea Institute of Science and Technology)
- 발행 : 2003.04.01
초록
Sphingolipid species are important second messengers due to their role in the mitogenesis, differentiation and apoptosis. We developed a new column liquid chromatography-triple quadrupole tandem mass spectrometry (LC-MS/MS) in combination with multiple reaction monitoring (MRM) method for the rapid, simultaneous and quantitative determination of unambiguous detecting sphingolipids in cell culture of human cancer cells (HL-60). (omitted)
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