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http://dx.doi.org/10.7314/APJCP.2014.15.13.5417

Metabolomics Investigation of Cutaneous T Cell Lymphoma Based on UHPLC-QTOF/MS  

Zhou, Qing-Yuan (School of Pharmacy, Fudan University)
Wang, Yue-Lin (School of Pharmacy, Fudan University)
Li, Xia (School of Pharmacy, Fudan University)
Shen, Xiao-Yan (Department of Dermatology, Ruijin Hospital, School of Medicine, Shanghai Jiaotong University)
Li, Ke-Jia (Department of Dermatology, Ruijin Hospital, School of Medicine, Shanghai Jiaotong University)
Zheng, Jie (Department of Dermatology, Ruijin Hospital, School of Medicine, Shanghai Jiaotong University)
Yu, Yun-Qiu (School of Pharmacy, Fudan University)
Publication Information
Asian Pacific Journal of Cancer Prevention / v.15, no.13, 2014 , pp. 5417-5421 More about this Journal
Abstract
Objectives: The identification of cutaneous T cell lymphoma (CTCL) biomarkers may serve as a predictor of disease progression and treatment response. The aim of this study was to map potential biomarkers in CTCL plasma. Design and Methods: Plasma metabolic perturbations between CTCL cases and healthy individuals were investigated using metabolomics and ultrahigh performance liquid chromatography-quadrupole time-of-flight mass spectrometry (UHPLC-QTOF/MS). Results: Principal component analysis (PCA) of the spectra showed clear metabolic changes between the two groups. Thirty six potential biomarkers associated with CTCL were found. Conclusions: Based on PCA, several biomarkers were determined and further identified by LC/MS/MS analysis. All of these could be potential early markers of CTCL. In addition, we established that heparin as a nticoagulant has better pre-treatment results than EDTA with the UHPLC-QTOF/MS appraoch.
Keywords
Cutaneous T cell lymphoma; metabolomics; biomarkers; UHPLC-QTOF/MS;
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1 Xia YH, Li m, Fu DD et al (2013). Effects of PTTG down-regulation on proliferation and metastasis of the SCL-1 cutaneous squamous cell carcinoma cell line. Asian Pac J Cancer P, 14, 6245-48.   과학기술학회마을   DOI
2 Yin PY, Andreas P, Holger F et al (2013). Preanalytical aspects and sample quality assessment in metabolomics studies of human blood. Clin Chem, 59, 5833-845.
3 Chuang TY, Su WP, Muller SA (1990). Incidence of cutaneous T cell lymphoma and other rare skin cancers in a defined population. J Am Acad Dermatol, 23, 254-6.   DOI   ScienceOn
4 Kim YH, Liu HL, Mraz-Gernhard, S et al (2003). Long-term outcome of 525 patients with mycosis fungoides and Sezary syndrome: clinical prognostic factors and risk for disease progression. Arch Dermatol, 139, 857-66.
5 Kunnathur MS, Chandrasekaran G (2013). Acacia ferruginea inhibits tumor progression by regulating inflammatory mediators-(TNF-a, iNOS, COX-2, IL-1$\beta$, IL-6,IFN-$\gamma$, IL-2, GM-CSF) and pro-angiogenic growth factor-VEGF. Asian Pac J Cancer P, 14, 3909-19   과학기술학회마을   DOI   ScienceOn
6 Li Ying, Yuan WR, Shen XY, Zheng J(2008). The comparison of the efficacy of skin directed therapy versus chemotherapy for CTCL. J Clin Dermatol, 37, 91-3.
7 Regina FP, Paulus Z, Barbara B et al (2002). Primary cutaneous lymphomas: applicability of current classification schemes (European Organization for Research and Treatment of Cancer, World Health Organization) based on clinicopathologic features observed in a large group of patients. Blood, 99, 800-5.   DOI   ScienceOn
8 Weinstock MA, Gardstein B (1999). Twenty year trends in the reported incidence of mycosis fungoides and associated mortality. Am J Public Health, 89, 1240-4.   DOI   ScienceOn
9 Yang W, Chen YH, Xi C et al (2013). Liquid chromatography-tandem mass spectrometry-based plasma metabolomics delineate the effect of metabolites' stability on reliability of potential biomarkers. Anal Chem, 85, 2606-10.   DOI   ScienceOn
10 Wu H, Xue RY, Tang ZQ et al (2010). Metabolomic investigation of gastric cancer tissue using gas chromatography/mass spectrometry. Anal Bioanal Chem, 396, 1385-95.   DOI