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Establishment of Analytical Method for Fenhexamid Residue in Korean Cabbage, Apple, Mandarin and Green Pepper (HPLC를 이용한 배추, 사과, 감귤, 고추 중 살균제 Fenhexamid의 정밀 분석법 확립)

  • Lee, Hye-Ri;Riu, Myoung-Joo;Park, Hee-Won;Na, Ye-Rim;Song, Hyuk-Hwan;Keum, Young-Soo;Zhu, Yongzhe;Kim, Jeong-Han
    • The Korean Journal of Pesticide Science
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    • v.13 no.4
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    • pp.223-231
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    • 2009
  • This study was performed to develop a precise single residue analytical method of fungicide fenhexamid in representative crops for general residue analytical method which could be applied to most of crops. Korean cabbage, mandarin, apple and green pepper were selected, macerated, extracted with acetone, concentrated and partitioned with dichloromethane. Then the extracts were concentrated and cleaned-up through Florisil column with ethyl acetate/0.1% acetic acid in hexane [15:85, (v/v)] before concentration and analysis with HPLC. LOQ (Limit of Quantitation) of fenhexamid was 1 ng (S/N>10) and MQL (Method Quantitative Limit) was 0.01 mg/kg. Recoveries were measured at two fortification levels (10 MQL and 50 MQL) on crop samples and ranged from 85.2% to 94.8% (mean recoveries) and coefficients of variation were <10% regardless of sample type.

Dimension- by-Dimension Effects of Service Quality on the Customer Relationship Quality of Internet Shopping Malls and the Moderating Role of the Length of Use (인터넷 쇼핑몰 고객관계의 질에 대한 서비스품질 차원별 영향 및 이용기간의 조절역할)

  • Quan Dong Mei
    • Journal of Distribution Research
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    • v.10 no.2
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    • pp.99-120
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    • 2005
  • First, based on extant studies in the field, the author extracts environments, general and personalized interactions between an internet shopping mall and its customers, interactions among customers, and outcomes as 5 relevant dimensions of the service quality of an internet shopping mal She derives hypotheses on the relationships between each of the service quality dimensions and each of the three components of the relationship quality, satisfaction, trust, and commitment. She also investigates the moderating role of the relationship duration defined by the length of use in the relationships. She studies the relationships among the three relationship quality components and their effects on the future intentions of relationship maintenance, too. Through a survey on college students and adult consumers, she gathers 349 qualified questionnaires. Applying regression methods on the data, she finds out that most of the relationships between each of the service quality dimensions and each of the relationship quality dimensions are statistically significant but that the strengths of relationship are quite different dimension by dimension. The moderating effect of the relationship duration is not found except one case, the relationship between general interactions and trust. The author also finds out positive relationships between satisfaction and trust between satisfaction and commitment, and between trust and commitment. Positive relationships between trust and the future intention of relationship maintenance and between commitment and the future intention are found. But the data does not reveal any statistically significant relationship beetween satisfaction and the future intention. The author provides a few implications of the research results and future research agenda.

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A Study of Korean Agri-Food Brand Identities for Expanding Exports to China (중국 수출확대를 위한 한국 농식품 브랜드 아이덴티티 정립)

  • An, Wook-Hyun;Cho, Woo-Chul;Kim, Chang-Hwan
    • Journal of Distribution Science
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    • v.12 no.2
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    • pp.7-16
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    • 2014
  • Purpose - The purpose of this study is to target Chinese consumers of Korean agri-food through the analysis of agri-food brand identity images to set future directions for proposed agri-food exports to China. Since negotiations began for a free trade agreement (FTA) between Korea and China, some Koreans have worried about the Korean agriculture sector. The world trading system has undergone many changes in recent years. Since the start of FTA negotiations on agriculture, China and Korea have faced a heightened sense of crisis. China is trying to export agricultural products so actively that its agricultural exports could increase by more than 7% annually. China exported about one-tenth of its agricultural products to Korea in the period 2002-2010. Recently, the importance of brand identity has tended to increase, as the world becomes one unified market. In this situation, we should try to promote agri-food exports to China. It is very important to understand consumers' recognition of Korean food and the image of the Korean agri-food industry, to establish an export strategy. Research design, data, and methodology - This study targeted 2,800 adult men and women aged 25-59 years living in four major cities in China (Beijing, Shanghai, Guangzhou, and Qingdao) with a household income within the top 10% level in the China, using the one-to-one interview survey method. In addition, four groups by region (eight people per group) for a total of 32 groups were surveyed by using the focus group interview (FGI) research method, and impressions of imported agri-food were surveyed using 17 questions about fresh agri-food and 22 questions on processed agri-food. Factor analysis, brand positioning, biplot analysis, and so on, were carried out based on the results of these surveys. This research presents the brand identity of Korean food; according to the analyses, the brand identity is based on trust, safety, and attractiveness. Result - Factor analysis results showed that Chinese consumers expect four major qualities in imported fresh agri-food: freshness/safety, attractive appearance, premium branding, and excellent taste/quality. In the case of processed agri-food, it turned out that Chinese consumers are focused on safety/hygiene/freshness, health, and taste. In addition, in the fresh agri-food analysis by country, American foods had the upper hand; the image of American foods was also recognized as the most positive for processed agri-food. Meanwhile, according to the biplot analysis, Korean agri-food is strongly preferred for its appearance, but no country is strongly preferred as regards the level of freshness/safety that is expected by Chinese consumers. Conclusions - In this study, we analyzed the image of imported agri-food expected by Chinese consumers by reconstructing agri-food characteristics from the brand perspective. The research result shows that a Korean brand identity must be the ultimate goal for activities undertaken to enhance the image of Korean agri-food in the future. Additional research is needed because brand image can be formed through various channels.

Bioconversion of Pinoresinol Diglucoside from Glucose Using Resting and Freeze-Dried Phomopsis sp. XP-8 Cells

  • Gao, Zhenhong;Rajoka, Muhammad Shahid Riaz;Zhu, Jing;Zhang, Zhiwei;Zhang, Yan;Che, Jinxin;Xu, Xiaoguang;Shi, Junling
    • Journal of Microbiology and Biotechnology
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    • v.27 no.8
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    • pp.1428-1440
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    • 2017
  • Phomopsis sp. XP-8 (an endophytic fungus) was previously found to produce pinoresinol diglucoside (PDG), a major antihypertensive compound of Tu-Chung (the bark of Eucommia ulmoides Oliv.), which is widely used in Chinese traditional medicines. In the present study, two bioconversion systems were developed for the production of PDG in Tris-HCl buffer containing glucose and Phomopsis sp. XP-8 cells (both resting and freeze-dried). When other factors remained unchanged, the bioconversion time, glucose concentration, cell ages, cell dosage, pH, temperature, and stirring speed influenced PDG production in a similar and decreasing manner after an initial increase with increasing levels for each factor. Considering the simultaneous change of various factors, the optimal conditions for PDG production were established as 70 g/l cells (8-day-old), 14 g/l glucose, $28^{\circ}C$, pH 7.5, and 180 rpm for systems employing resting cells, and 3.87 g/l cells, 14.67 g/l glucose, $28^{\circ}C$, pH 7.5, and 180 rpm for systems employing freeze-dried cells. The systems employing freeze-dried cells showed lower peak PDG production ($110.28{\mu}g/l$), but at a much shorter time (12.65 h) compared with resting cells (23.62 mg/l, 91.5 h). The specific PDG production levels were 1.92 and $24{\mu}g$ per gram cells per gram glucose for freeze-dried cells and resting cells, respectively. Both systems indicated a new and potentially efficient way to produce PDG independent of microbial cell growth.

An Empirical Study on the Measurement of Clustering and Trend Analysis among the Asian Container Ports Using the Variable Group Benchmarking and Categorical Variable Models (가변 그룹 벤치마킹 모형과 범주형 변수모형을 이용한 아시아 컨테이너항만의 클러스터링측정 및 추세분석에 관한 실증적 연구)

  • Park, Rokyung
    • Journal of Korea Port Economic Association
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    • v.29 no.1
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    • pp.143-175
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    • 2013
  • The purpose of this paper is to show the clustering trend by using the variable group benchmarking(VGB) and categorical variable(CV) models for 38 Asian ports during 9 years(2001-2009) with 4 inputs(birth length, depth, total area, and number of crane) and 1 output(container TEU). The main empirical results of this paper are as follows. First, clustering results by using VGB show that Shanghai, Qingdao, and Ningbo ports took the core role for clustering. Second, CV analysis focusing on the container throughputs indicated that Singapore, Keelong, Dubai, and Kaosiung ports except Chinese ports are appeared as the center ports of clustering. Third, Aqaba, Dubai, Hongkong, Shanghai, Guangzhou, and Ningbo ports are recommended as the efficient ports for the target of clustering. Fourth, when the ports are classified by the regional location, Dubai, Khor Fakkan, Shanghai, Hongkong, Keelong, Ningbo, and Singapore ports are the core ports for clustering. On the whole, other ports located in Asia should be clustered to Dubai, Khor Fakkan, Shanghai, Hongkong, Ningbo, and Singapore ports. The policy implication of this paper is that Korean port policy planner should introduce the VGB model, and CV model for clustering among the international ports for enhancing the efficiency of inputs and outputs.

Levels of common salivary protein 1 in healthy subjects and periodontal patients

  • Heo, Seok-Mo;Lee, Sol;Wang, HongTao;Jeong, Jeong Hyeok;Oh, Sang Wook
    • Journal of Periodontal and Implant Science
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    • v.46 no.5
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    • pp.320-328
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    • 2016
  • Purpose: Human saliva, as a vital part of the immune defense system, contains a number of distinct proteins and peptides. Recently human common salivary protein 1 (CSP1) has been identified as an abundant salivary protein and may play a role in promoting the binding of cariogenic bacteria to salivary pellicles. However, nothing else is known regarding the role of CSP1 in periodontology. The aim of this study was to quantify and compare CSP1 levels between healthy subjects and periodontal patients. Methods: This controlled clinical study was conducted in periodontally healthy individuals and patients with chronic periodontitis Chonbuk National University Hospital, with Institutional Review Board approval. Whole saliva samples were collected from 36 healthy subjects and 33 chronic periodontitis patients and analyzed. Sodium dodecyl sulfate-polyacrylamide gel electrophoresis and immune blotting were conducted to ensure that anti-CSP1 monoclonal antibody (mAb) binds to CSP1 in human saliva. A sandwich enzyme-linked immunosorbent assay (ELISA) system was house-fabricated using mAb-hCSP1#14 and mAb-hCSP1#4 as a capture and a detector mAb, respectively. The CSP1 concentrations in saliva from 36 healthy subjects and 33 periodontal patients were quantified using the CSP1 sandwich ELISA system, and the results were analyzed using the Student's t-test. Results: Immunoblot analysis using mAb-hCSP1 as a probe confirmed that CSP1 in human saliva existed as a single band with a molecular weight of approximately 27-kDa. The quantification of CSP1 concentrations by CSP1 ELISA showed that the median values (25th to 75th percentiles) of periodontal patients and healthy subjects were 9,474 ng/mL (range, 8,434.10,139 ng/mL) and 8,598 ng/mL (range, 7,421.9,877 ng/mL), respectively. The Student's t-test indicated the presence of a statistically significant difference between the 2 groups (P=0.024). Conclusions: The presence of a significant difference in CSP1 levels between healthy subjects and periodontal patients suggests that CSP1 may be a potential biomarker for the detection or screening of periodontitis patients.

HGFK1 is Associated with a Better Prognostis and Reverses Inhibition by Gefitinib in NSCLC Cases

  • Zhou, Xiao-Hui;Tang, Li-Na;Yue, Lu;Min, Da-Liu;Yang, Yi;Huang, Jian-An;Shen, Zan
    • Asian Pacific Journal of Cancer Prevention
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    • v.13 no.4
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    • pp.1457-1461
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    • 2012
  • Purpose: Non small cell lung cancer (NSCLC) is the leading worldwide source of cancer-related deaths. Although some drugs targeting EGFR mutations have been developed, most advanced cases are still incurable. New targets for anticancer drugs are demanded. The kringle 1 domain of hepatocellular growth factor alpha chain (HGFK1) is a potent anti-angiogenesis factor. It has also emerged as a potential anticancer factor in hepatocellular carcinoma (HCC). The expression of HGFK1 protein in patients with NSCLC has not been reported to date. Method: Here, we assessed HGFK1 expression by Western blotting in 103 cases with advanced NSCLC to investigate the impact of HGFK1 on survival. Results: Results revealed 33 (30.1%) patients were classified as high expressors, this being significantly associated with less remote metastasis (P = 0.002) but not with lymph node metastasis (P = 0.062). There was also a significant association between HGFK1 expression and tumor size (P = 0.025) as well as clinical stage (P = 0.012). Kaplan-Meier survival analysis showed that both overall survival (OS) and progression free survival (PFS) of patients with HGFK1 expression were longer than those of patients without HGFK1 expression (P = 0.004 and P = 0.001 respectively). HGFK1 reversed gefitinib inhibition in the resistent NSCLC cell line A431/GR but did not inhibit the proliferation of NSCLC cells A431 and A431/GR directly. Reversion of gefitinib inhibition in A431/GR cells by HGFK1 was related to decreased phosphorylation of ERK and STAT5. Conclusions: HGFK1 may be a useful prognostic factor of advanced NSCLC patients and a potential drug for gefitinib resistant patients.

A Study on the Aesthetics of death in Alexander McQueen's works (알렉산더 맥퀸(Alexander McQueen) 작품에 나타난 죽음의 미학)

  • Wang, Xin-yu;Kim, Hyun-joo
    • Journal of Digital Convergence
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    • v.18 no.5
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    • pp.455-463
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    • 2020
  • This researcher has sorted out the development process of death aesthetics contained in human art through advanced research. This research is a follow-up study of related research, which studies the aesthetic meaning of death in Alexander McQueen's fashion works. This study first classifies the aesthetic characteristics of death, and then analyzes and sorts out the aesthetics of death in Alexander McQueen's clothing works based on this. The results of this study are as follows: first, the aesthetic manifestations of death are divided into three categories: reproducibility, symbolic and religious. Among them, the representation of reproducibility includes the representation of real objects and the representation of virtual objects, and the symbolic performance includes two types of external performance and internal performance. Second, in Alexander McQueen's works, the reproducibility of death was mainly reflected by virtual stage scenes and costumes. Thirdly, the symbolic death is not only reflected in the skeletons and blood etc. directly related to the death of the body, but also in the various emotions and psychological states caused by the death. Fourth, the religious nature of death was represented in Alexander McQueen's works through such representative things as crosses and religious stories.

The Analysis of Competitiveness in Container Ports of Shanghai and North China & Korea Using Inverse Relation of Fuzzy Evaluation and Scenario Analysis (퍼지 역평가법과 시나리오 분석을 통한 상하이 및 북중국과 우리나라 컨테이너항만의 경쟁력분석에 관한 연구)

  • Ryu, Hyung-Geun;Lee, Hong-Girl;Yeo, Ki-Tae
    • Journal of Korean Society of Transportation
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    • v.22 no.7 s.78
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    • pp.49-59
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    • 2004
  • In order to be a hub-port in Northeast Asia, current China government has intensively invested in port development. Further, this development Project is significantly big scale, compared with those projects which Korea and Japan have. Thus, China is beginning to threaten Korean ports, especially Busan port which try to be a hub port in Northeast Asia. For this reason, recently many studies to evaluate competitiveness between Korean ports, especially Busan and Gwangyang, and Chinese ports have been conducted. In the mean time, implications of those pervious research has mainly been based on evaluation of port competitiveness using evaluation methodologies, such as AHP(Analytical Hierarchy Process) and HFP(Hierarchical Fuzzy Process). However, as previous evaluation algorithms are methodologies that only calculate ranking of ports by competitiveness level, from the results of analysis, critical weak points affected current port competitiveness could not clearly fine out. That is, because there has not been any algorithm that can extract critical points from the evaluation results. The aim of this paper is to present critical points that affect port competitiveness using an algorithm based on IRFE(Inverse Relation of Fuzzy Evaluation), and scenario analysis, from previous results of evaluation of port competitiveness. And The research scope is to covey the subjective ports of Korea and China's 7 major ports (Busan, Gwangyang, Sanghai, Qingdao, Tienjin, Dalian and Kaoshuing). From analysis, it was found that critical weak point of Busan port is the level of hinterland including availability of free trade zone.

Physico-chemical Characteristics of Submicron Aerosol at West Inflow Regions in the Korean Peninsula III. Physical-Chemical Behavior and Long-range Transport of PM1 (한반도 서부유입권역에서 대기 중 에어로졸 성분의 물리·화학적 특성 연구 III. 화학적 거동 및 장거리 이동)

  • Park, Taehyun;Ahn, Junyoung;Choi, Jinsoo;Lim, Yongjae;Park, Jinsoo;Kim, Jeongho;Oh, Jun;Lee, Yonghwan;Hong, Youdeog;Hong, Jihyung;Choi, Yongjoo;Lee, Taehyoung
    • Journal of Korean Society for Atmospheric Environment
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    • v.33 no.2
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    • pp.124-138
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    • 2017
  • Physico-chemical measurement of non-refractory submicron particles($NR-PM_1$) was conducted in Baengnyeong Island, Korea using Aerodyne High Resolution Time of Flight Aerosol Mass Spectrometer (HR-ToF-AMS) from 2012 to 2014. Organics and ammoniated sulfate were dominant species in $NR-PM_1$. The organics was found to have similar fractions(approximate 40%) of $NR-PM_1$ during the summer and winter, while the sulfate fractions of $NR-PM_1$ were calculated to be approximately 47% and 31% for the summer and winter, respectively, suggesting the possibility that particles provide non-acidic surfaces for condensation of nitric acid in the winter. The nitrate fractions of approximate 4% and 20% of $NR-PM_1$ were observed in August (summer) and November (winter), respectively, resulting that the relatively low concentration of sulfate in $NR-PM_1$ provided a non-acidic surface for nitric acid condensation and formation of particulate ammoniated nitrate is favored thermodynamically in winter. The new particle formation (NPF) event and particle growth rate were analyzed for each month in 2014 using Scanning Mobility Particle Sizer(SMPS). The Percent of NPF events was the highest in winter, but NPF event was not observed during summer due to relatively high temperature and frequent rainfall. The average particle growth rate was 3.5 nm/h and the highest particle growth rate was 5.5 nm/h in May. We observed the long-range transport of the anthropogenic sulfate from the East Asia during the intensive monitoring period of November between Qingdao and Baengnyeong Island in 2013. The relatively high concentrations of m/z 60 measured in HR-ToF-AMS was observed in May and June at Baengnyeong Island, suggesting the possibility of the influence of biomass burning from the East Asia to the Korean Peninsula.