• Membrane and Water Treatment
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• 제11권4호
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• pp.247-255
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• 2020

Sand filter is a key unit process for particle removal in water purification treatments. Its long-standing use is due to on-site customized retrofit. Proper selection of filter media is one of the retrofit approaches to improve filter performance. This study described a series of controlled laboratory column tests and examined the effects of media property on filtration and backwash. When sand media of 0.51 mm in effective size was replaced by sand of 0.60 mm, the filter run increased up to 5 times in the given bed depth. The change of media property required an increase of backwash rate by 0.05 m/min to satisfy the requirement of bed expansion, more than 20%. When the anthracite was changed with lower effective size and uniformity coefficient, correlation with sand in the filter bed could be satisfied within the permissible error between media and bulk characteristics. Besides, this selection resulted in a well-stratified configuration of media layers after bed expansion. The column study showed that the correlation of property between the dual media had a significant effect on the filter productivity and backwash interval.

• 한국식물병리학회지
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• 제14권1호
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• pp.62-67
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• 1998

Barley yellow mosaic virus (BaYMV-HN) occurring Haenam area was isolated by mechanical inoculation onto barley cultivars, purification and production of antibody. BaYV-HN were purified from infected plants a filamentous virus with 13 nm in diameter and 250∼300 nm and 500∼650 nm in length. Specific antibody made by injecting the purified virus to the muscle of a rabbit. In gel-diffusion tests antibody to BaYMV-HN did not make spur with tow Japanese BaYMV isolates BaYMV-II-1 or BaYMV-III. BaYMV-HN showed the symptom of yellowing and necrosis in host plants. Mechanical inoculation tests with Japanese barley cultivars showed that BaYMV-HN infected New Golden, Akagi Nijo and Tosan Kawa 73, but did not infect Amagi Nijo, Haruna Nijo, Ishukushirazu (ym3), Misato Golden (Ym1), Kashimamugi, Joshushiro Hadaka and Mokusekko 3 (ym1). In Korean barley cultivars, some of the naked barleys which are Hinssalbori, Kinssalbori, Saessalbori and Saechalssalbori were not infected by BaYMV-HN. However, it infected all the covered barley cultivars and the beer barley cultivars. BaYMV-HN had two RNAs, RNA 1 (7.6 Kb) and RNA 2 (3.5 Kb), and one coat protein (33 KDa).

• 한국식물병리학회지
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• 제14권1호
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• pp.68-73
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• 1998

Barley mild mosaic virus (BaMMV-Kor) was isolated from the southern part of Korea, and by mechanical inoculation onto barley cultivars, purification and production of antibody. BaMMV-Kor purified form infected plants were filamentous particle, with 13 nm in diameter and 250∼300 nm and 500∼650 nm in length. Antibody of BaMMV-Kor was made by injecting the purified virus to the muscle of a rabbit. In gel-diffusion test, antibody to BaMMV-Kor created spur with BaMMV-Kal and BaMMV-M, but did not make spur with BaMMV-Kor infected New Golden, Ishukushirazu, Joshushiro Hadaka and Misato Golden, but did not infect Kashimamugi, Chikurin Ibaraki 1 and Mokusekko 3. In Korean barley cultivars, BaMMV-Kor infected most of the covered barley cultivars, but did not infect Saeolbori. It also infected naked barley cultivars except Chalbori and Hinssalbori. And all the beer barley cultivars were infected by BaMMV-Kor. BaMMV-Kor had two RNAs, RNA 1 (7.5 Kb) and RNA 2 (3.5 Kb), and coat protein (33 KDa).

• 한국식물병리학회지
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• 제13권2호
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• pp.73-78
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• 1997

A virus was purified from Korean cactus (Chamaeaereus silverstrii f. Variegata Hort) showing necrotic spots and its biophysicochemical properties were examined. The typical necrotic spots were observed on healthy looking cactus inoculated with the purified virus and the various symptoms from necrotic spots to systemic were observed on 17 species of test plats used, which suggests that the purified virus has the normal infectivity and wide host range. Ultraviolet absorption scanning analysis revealed that the viral preparation had the typical curve with shoulder at 290 nm and its ratio of absorbances at 260 nm to 280 nm and extinction coefficient was 1.156 and 2.86, respectively. The virus represented one distinct and homogeneous band and molecular weight of viral coat protein was determined to 17, 000 Dalton by SDS-PAGE. The virus revealed rod-shaped virion (325$\times$20 nm) by electron microscopy. Based on these results, it has been thought that the virus purified in this study is the pathogenic agents causing necrotic spot symptom on Korean cacuts and one of the member of tobamovirus family.

• Applied Biological Chemistry
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• 제12권
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• pp.1-5
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• 1969

1. 한국산(韓國産) 대두(大豆)에서 soybean hemagglutinin 을 분리(分離) 정제(精製)하였으며 이것의 당조성(糖組成)이 mannose(4.9%) glucosamine(1.1%)임을 확인(確認)하였다. 2. Crude soybean hemagglutinin 의 Column chromatography에 의한 정제(精製)도중 분리(分離)되는 불순물(不純物)인 Fraction I,II,III는 여지전기영동 상으로는 같은 이동도(移動度)를 갖고 있는 새로운 Plant glycoprotein들로서 당부분(糖部分)은 mannose 4.5%, 1.13%, 1.1%, glucosamine 0.5%, 1.2%, 1.22%이고 질소함량은 12.8%, 15.5%, 13.9%이었다.

• 식물병연구
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• 제13권1호
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• pp.30-33
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• 2007

석량풋콩 품종의 콩밭에서 모자이크 증상이 약하게 나타난 186개의 잎을 채집하여, Cowpea mosaic virus(CPMV)에 대한 ELISA 검정을 하였다. 그 결과 93개의 시료가 양성반응을 보였으며, 이 가운데 55개의 시료는 매우 높은 바이러스 밀도를 나타내고 있었다. 이러한 결과는 석량풋콩 품종의 동부모자이크병은 ELISA 검정, 바이러스 순화, 전자현미경검경 결과와 바이러스 표피 단백질분석 결과 Cowpea mosaic virus에 의한 병해로 확인되었다.

• 한국토양비료학회지
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• 제44권3호
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• pp.434-441
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• 2011

축산농가에서 소규모로 발생하는 고농도의 질소와 인을 함유한 축산폐수의 처리효율을 극대화시키기 위해 수생식물여과조-활성슬러지조-인공습지로 구성된 축산폐수처리 시스템에서 축산폐수 재주입 비율별 및 주입방법별 수처리 효율을 조사하였다. 축산폐수의 재주입 비율별에 따른 처리효율을 조사한 결과 COD, SS, T-N 및 T-P 처리효율은 30% < 70% ${\leq}$ 100%의 순으로 재주입 비율이 증가함에 따라 오염물질의 처리효율이 점점 증가하는 경향이었다. 또한, 주입방법에 따른 COD, SS, T-N 및 T-P의 처리효율을 조사한 결과 1차 및 2차 처리조까지는 연속적 주입이 간헐적 주입에 비해 약간 높은 처리효율을 보였으나, 3차 처리조 부터는 비슷한 처리효율을 보였으며, 방류수 중COD, SS, T-N 및 T-P의 처리효율은 연속주입방법이 각각 99.5, 99.8, 99.0 및 99.8%로 간헐주입에 비해 약간 높았다. 이들 결과를 미루어 볼 때, 본 현장 인공습지 축산 폐수처리장에서 은 적합한 축산폐수의 재주입 비율과 주입방법을 선정한다면 질소 및 인 처리효율을 향상 시킬 수 있을 것으로 판단된다.

• Nuclear Engineering and Technology
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• 제49권7호
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• pp.1489-1494
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• 2017

The use of a room-temperature gamma spectrometer is an issue in environmental radiation monitoring. To monitor radionuclides released around a nuclear power plant, suitable instruments giving fast and reliable information are required. High-pressure xenon (HPXe) chambers have range of resolution and efficiency equivalent to those of other medium resolution detectors such as those using NaI(Tl), CdZnTe, and $LaBr_3:Ce$. An HPXe chamber could be a cost-effective alternative, assuming temperature stability and reliability. The CEA LIST actively studied and developed HPXe-based technology applied for environmental monitoring. Xenon purification and conditioning was performed. The design of a 4-L HPXe detector was performed to minimize the detector capacitance and the required power supply. Simulations were done with the MCNPX2.7 particle transport code to estimate the intrinsic efficiency of the HPXe detector. A behavioral study dealing with ballistic deficits and electronic noise will be utilized to provide perspective for further analysis.

• 환경위생공학
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• 제10권1호
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• pp.126-131
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• 1995

Chitinase, a potential pathogensis- related portein, was induced in the leaves of 30 day - old bean( phaseolus vulgaris ) plant with the treatment of 10 $\mu $ /mℓ ethylen for 30hrs. Chitinase was purified from the mature tissue of bean leaves( phaseolus vulgaris ) by ammonium sulfate precipitation followed by affinity chromatography on a regenerated chitin and sephadex G-75 chromatography. The purified chitinase gave a single band SDS- PAGE to be 32,000 Dalton. In order to elucidate the three- dimensional structure of chitinase and to shed light on the functional mechanism of this class of enzymes, the enzyme was tried to crystallize( Sitting Drop Method). Crystals grew at room temperature to their final size within two weeks(0.2mm $\times $0.2mm $\times $ 0.1 mm ), This enzyme are still continuing to crystallize for the study of X- ray.

• Journal of Advanced Marine Engineering and Technology
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• 제29권5호
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• pp.488-494
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• 2005

The ejector is used to obtain a vacuum state, and it has been applied to a lot of industry field such as a heat engine, a fluid instrument power plant. a food industry, an environment industry etc., because there is no problem even it is mixed with any kind of liquid, gas. and solid. The flow characteristics in the ejector was investigated by a PIV and a CFD. The agreement between numerical analysis and experiment shows the validity of this study and the results of this study would be useful to the engineers who design for the flow systems for heating. ventilation. air conditioning and wastewater purification plants.