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http://dx.doi.org/10.5423/RPD.2007.13.1.030

Severity of Cowpea mosaic virus and Putkong Disease Monitoring and Purification of Cowpea mosaic virus  

Cho, Eui-Kyoo (Major in Agricultural Biology, School of Bioresource Science, Andong National University)
Publication Information
Research in Plant Disease / v.13, no.1, 2007 , pp. 30-33 More about this Journal
Abstract
One hundred and eighty-six leaves of soybean cv. Seokryangputkong that showed mild mosaic symptoms were collected randomly and ELISA tests were conducted with those leaf samples to screen the presence of Cowpea mosaic virus (CPMV). Ninety-three out of 186 samples reacted positively to CPMV, but those samples did negatively to Soybean mosaic virus (SMV). At least, 55 leaf samples revealed higher values than that of positive control. The results strongly confirmed that CPMV occurred severely in soybean cv. Seokryangputkong. However, a question is raised on the primary reservoir and vector for transmission of this virus. Since the farmer changes seeds every year, seed transmission is excluded. The virus was also purified, the analysis of coat protein conformed the virus of cowpea mosaic virus and UV absorption pattern confirmed that the causal virus of mosaic disease in soybean putkong was cowpea mosaic virus.
Keywords
Soybean cv.'Seokryangputkong'; ELISA; Cowpea mosaic virus (CPMV); Soybean mosaic virus (SMV);
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