Korean Journal Plant Pathology (한국식물병리학회지)
- Volume 13 Issue 2
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- Pages.73-78
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- 1997
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- 0256-8608(pISSN)
Purification and Biophysicochemical Properties of the Virus Causing Necrotic Spot Symptom on Korean Cactus
한국산 접목 선인장에서 분리한 괴사 반점 원인 바이러스의 생물리화학적 특성
Abstract
A virus was purified from Korean cactus (Chamaeaereus silverstrii f. Variegata Hort) showing necrotic spots and its biophysicochemical properties were examined. The typical necrotic spots were observed on healthy looking cactus inoculated with the purified virus and the various symptoms from necrotic spots to systemic were observed on 17 species of test plats used, which suggests that the purified virus has the normal infectivity and wide host range. Ultraviolet absorption scanning analysis revealed that the viral preparation had the typical curve with shoulder at 290 nm and its ratio of absorbances at 260 nm to 280 nm and extinction coefficient was 1.156 and 2.86, respectively. The virus represented one distinct and homogeneous band and molecular weight of viral coat protein was determined to 17, 000 Dalton by SDS-PAGE. The virus revealed rod-shaped virion (325
Keywords
- Chamaeaereus silverstriif. variegata hort;
- necrotic spots;
- biophysicochemical properties;
- SDS-PAGE;
- spectrophotometry;
- electron microscopy.