• Journal of Microbiology and Biotechnology
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• 제24권1호
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• pp.59-69
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• 2014

One of the major challenges in metabolic engineering for enhanced synthesis of value-added chemicals is to design and develop new strains that can be translated into well-controlled fermentation processes using bioreactors. The aim of this study was to assess the influence of various fed-batch strategies in the performance of metabolically engineered Pseudomonas putida strains, ${\Delta}gcd$ and ${\Delta}gcd-pgl$, for improving production of medium-chain-length polyhydroxyalkanoates (mcl-PHAs) using glucose as the only carbon source. First we developed a fed-batch process that comprised an initial phase of biomass accumulation based on an exponential feeding carbon-limited strategy. For the mcl-PHA accumulation stage, three induction techniques were tested under nitrogen limitation. The substrate-pulse feeding was more efficient than the constant-feeding approach to promote the accumulation of the desirable product. Nonetheless, the most efficient approach for maximum PHA synthesis was the application of a dissolved-oxygen-stat feeding strategy (DO-stat), where P. putida ${\Delta}gcd$ mutant strain showed a final PHA content and specific PHA productivity of 67% and $0.83g{\cdot}l^{-1}{\cdot}h^{-1}$, respectively. To our knowledge, this mcl-PHA titer is the highest value that has been ever reported using glucose as the sole carbon and energy source. Our results also highlighted the effect of different fed-batch strategies upon the extent of realization of the intended metabolic modification of the mutant strains.

• 대한전자공학회논문지SD
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• 제39권8호
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• pp.1-11
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• 2002

다전극 DFB-LD는 외부에서 주입되는 광의 파워에 따라서 발진하는 광출력이 쌍안정성을 보임에 따라, 이를 파장 변환에 활용하거나, 광논리 소자로 활용할 수 있는 가능성이 있다. 본 논문에서는 연산자 분리 시영역 모델을 이용하여 다전극 DFB-LD에서의 광 쌍안정 특성에 대하여 연구하였다. 다전극 DFB-LD에 불균등하게 전류를 인가하여 쌍안정 현상이 발생함을 확인하고, 흡수 영역으로 입력광을 입사하였을 때 발생하는 출력광 파워의 쌍안정 현상도 확인하였다. 그리고 수 ns의 스위칭 시간과 수 pj의 스위칭 에너지를 가진 set 또는 reset 광 펄스의 인가에 의하여 flip-flop 특성을 보임에 따라 광 메모리 소자로서의 동작도 확인하였다. 또한 캐리어 생존시간과 이득 곡선 기울기 등의 조절로서 LD 광 출력의 반응 시간을 줄일 수 있는 가능성을 확인하였다.

• 전기화학회지
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• 제18권3호
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• pp.130-135
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• 2015

흑연 분말을 프탈로시아닌 또는 구리 프탈로시아닌과 함께 비활성 분위기에서 각각 열처리하여 표면처리를 진행하였고, 이의 속도특성과 저온 작동특성을 조사하였다. 표면처리 후 흑연 분말의 표면에 비정질 탄소와 구리의 코팅 층이 균일하게 형성되었다. 표면처리를 통하여 흑연 전극의 속도특성이 개선되는 것을 확인하였는데, 특히 구리 프탈로시아닌으로 처리한 경우 속도특성의 향상이 두드러졌다. 흑연 전극의 저항을 교류 임피던스와 펄스 저항측정법을 활용하여 조사하였는데, 구리 프탈로시아닌으로 처리된 흑연 전극의 경우가 저항이 가장 작았다. 프탈로시아닌으로 부터 유도된 비정질 탄소 층이 리튬이온의 확산을 용이하게 하고, 구리 프탈로시아닌으로부터 유도된 금속상태의 구리는 전자 전도도를 증가시키기 때문에 저항을 감소시키는 것으로 판단된다.

• 한국전기전자재료학회:학술대회논문집
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• 한국전기전자재료학회 2008년도 하계학술대회 논문집 Vol.9
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• pp.297-297
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• 2008

Buffer layers play an important role in the development of high critical current density coated conductor. $LaMnO_3$, $SrTiO_3$ and $BaZrO_3$ buffer layers were compatible with MgO surfaces and also provide a good template for growing high current density REBCO(RE=Rare earth) films. Systematic studies on the influences of pulsed laser deposition parameters (deposition temperature, deposition pressure, processing gas, laser energy density, etc.) on microstructure and texture properties of $LaMnO_3$, $SrTiO_3$ and $BaZrO_3$ films as buffer layer deposited on ion-beam assisted deposition MgO (IBAD_MgO) template by pulse laser deposition method, were carried out. These results will be presented together with the discussion on the possible use of this material in HTS coated conductor as buffer.

• 청정기술
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• 제13권2호
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• pp.115-121
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• 2007

본 연구에서는 유전체 장벽 방전-광촉매 복합 공정에 의한 NO 및 $SO_2$ 제거를 실험적으로 분석하였다. 유전체 장벽 방전을 위해 유전체로서 유리구가 사용되었고 $TiO_2$ 광촉매 입자는 딥코팅(dip-coating) 방법에 의해 높은 비표면적을 가지는 스펀지 형태로 유리구에 코팅되었다. 플라즈마 반응기에 인가된 전압이나 펄스 주파수, 혹은 기체의 체류시간이 증가함에 따라, NO 및 $SO_2$의 제거효율은 증가하였다. NO 및 $SO_2$ 공급농도 증가하면 NO 및 $SO_2$ 제거에 더 많은 에너지가 요구되어 NO 및$SO_2$의 제거효율이 감소하였다. 본 연구의 실험 결과들은 NO 와 $SO_2$를 제거하기 위한 유전체 장벽 방전-광촉매 복합 공정 설계의 기초 자료로 사용될 수 있다.

• Structural Engineering and Mechanics
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• 제24권3호
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• pp.375-393
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• 2006

Under high velocity, pulse type near source earthquakes semi-active control systems are very effective in reducing seismic response base isolated structures. Semi-active control systems can be classified as: 1) independently variable stiffness, 2) independently variable damping, and 3) combined variable stiffness and damping systems. Several researchers have studied the effectiveness of independently varying damping systems for seismic response reduction of base isolated structures. In this study effectiveness of a combined system consisting of a semi-active independently variable stiffness (SAIVS) device and a magnetorheological (MR) damper in reducing seismic response of base isolated structures is analytically investigated. The SAIVS device can vary the stiffness, and hence the period, of the isolation system; whereas, the MR damper enhances the energy dissipation characteristics of the isolation system. Two separate control algorithms, i.e., a nonlinear tangential stiffness moving average control algorithm for smooth switching of the SAIVS device and a Lyapunov based control algorithm for damping variation of MR damper, are developed. Single and multi degree of freedom systems consisting of sliding base isolation system and both the SAIVS device and MR damper are considered. Results are presented in the form of nonlinear response spectra, and effectiveness of combined variable stiffness and variable damping system in reducing seismic response of sliding base isolated structures is evaluated. It is shown that the combined variable stiffness and variable damping system leads to significant response reduction over cases with variable stiffness or variable damping systems acting independently, over a broad period range.

• Smart Structures and Systems
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• 제25권1호
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• pp.65-80
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• 2020

In order to protect a structure over its full life cycle, a novel tuned mass damper (TMD), the so-called semi-active eddy current pendulum tuned mass damper (SAEC-PTMD), which can retune its frequency and damping ratio in real-time, is proposed in this study. The structural instantaneous frequency is identified through a Hilbert-Huang transformation (HHT), and the SAEC-PTMD pendulum is adjusted through an HHT-based control algorithm. The eddy current damping parameters are discussed, and the relationship between effective damping coefficients and air gaps is fitted through a polynomial function. The semi-active eddy current damping can be adjusted in real-time by adjusting the air gap based on the linear-quadratic-Gaussian (LQG)-based control algorithm. To verify the vibration control effect of the SAEC-PTMD, an idealized linear primary structure equipped with an SAEC-PTMD excited by harmonic excitations and near-fault pulse-like earthquake excitations is proposed as one of the two case studies. Under strong earthquakes, structures may go into the nonlinear state, while the Bouc-Wen model has a wild application in simulating the hysteretic characteristic. Therefore, in the other case study, a nonlinear primary structure based on the Bouc-Wen model is proposed. An optimal passive TMD is used for comparison and the detuning effect, which results from the cumulative damage to primary structures, is considered. The maximum and root-mean-square (RMS) values of structural acceleration and displacement time history response, structural acceleration, and displacement response spectra are used as evaluation indices. Power analyses for one earthquake excitation are presented as an example to further study the energy dissipation effect of an SAECPTMD. The results indicate that an SAEC-PTMD performs better than an optimized passive TMD, both before and after damage occurs to the primary structure.

• 대한기계학회논문집A
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• 제36권1호
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• pp.37-42
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• 2012

LIFT(Laser-Induced-Forward-Transfer) 공정은 선택적으로 마이크로 크기의 물질을 이동시키는데 사용할 수 있는 레이저 공정이다. 본 연구에서 이 공정은 전자부품의 마이크로 시스템 패키징을 위한 솔더볼의 적층을 위해 적용되었다. 레이저 펄스에 의해 국부적으로 녹은 솔더 페이스트는 단단한 기저부분에 이동적층되었다. 솔더 크림층을 지닌 박판유리플레이트가 도우너 필름으로 사용되었고 1070nm 파장의 IR 레이저 펄스를 조사하여 리셉터에 마이크로 솔더를 이동적층하였다. 물질 및 에너지 평형 방정식 등이 솔더 페이스 드롭의 모양과 온도 분포를 분석하기 위해 적용되었다. 실제 실험에서 얻어진 이동적층된 솔더 범프는 30~40 ${\mu}m$ 의 직경과 50 ${\mu}m$ 의 두께를 가진 것으로 측정되었다. 본 공정의 한계 및 적용에 대해서도 논의한다.

• 전기학회논문지
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• 제66권7호
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• pp.1066-1071
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• 2017

Recently high voltage impulse (HVI) technique has been extensively studied for desalting processes to control the $CaCO_3$ scale formation in industrial water practices such as power plant, boiler, and heat exchange operations. Investigation of the operational parameters for the HVI is important, however, those had not been reported yet. In this study, the effect of initial feed volume and contact time on reduction of calcium ion concentration by the HVI technique was investigated. Initial feed volumes of artificial hard water which contained 100 mg/L of $Ca^{2+}$, were set to 1, 2, and 3 L respectively. After 24hr of HVI contact with 12kV, $Ca^{2+}$ ion was reduced to 50, 29 and 19 % of their initial concentration, indicating that calcium removal increased as initial feed volume decreased. This implies the applied HVI pulse energy per unit mass of calcium is important parameter determining overall desalting efficiency. A series of extended operations of HVI up to 30 days verified the long term stability of the HVI system. The calcium ion declined to 40 mg/L after 2~3 days, and further reduction of calcium was not achieved, indicating that optimum operation time could be 2~3 days under these experimental conditions. Consequently, it was confirmed that the important operational parameter of HVI technique is initial feed volume and contact time as well as the applied voltage that was already proven in the previous study.

• 한국군사과학기술학회지
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• 제15권2호
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• pp.201-207
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• 2012

Pulsed lasers at the 613 nm and 1064 nm wavelengths on nanoseconds have been utilized to characterize the damage on Si photodiode exposed to intense illumination. Morphological damages and structural changes at sites on the photodiode irradiated during microseconds of laser pulses were analyzed by FE-SEM images and XRD patterns, respectively. The removal of oxide coating, ripple, melting marks, ridges, and crater on photodiodes were definitely observed in order of increasing the pulse intensities generated above the damage threshold. Then, the degradation in photosensitivity of the Si photodiode irradiated by high power density pulses was measured as a function of laser irradiation time at the various wavelengths. The free charge carrier and thermal diffusion mechanisms could have been invoked to characterize the damage. The relative photosensitivity data calculated using the thermal diffusion model proposed in this paper have been compared with the experimental data irradiated above the damage threshold.