• The Plant Pathology Journal
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• 제38권6호
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• pp.603-615
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• 2022

Soybean (Glycine max (L) Merr.) provides plant-derived proteins, soy vegetable oils, and various beneficial metabolites to humans and livestock. The importance of soybean is highly underlined, especially when carbon-negative sustainable agriculture is noticeable. However, many diseases by pests and pathogens threaten sustainable soybean production. Therefore, understanding molecular interaction between diverse cultivated varieties and pathogens is essential to developing disease-resistant soybean plants. Here, we established a pathosystem of the Korean domestic cultivar Kwangan against Pseudomonas syringae pv. syringae B728a. This bacterial strain caused apparent disease symptoms and grew well in trifoliate leaves of soybean plants. To examine the disease susceptibility of the cultivar, we analyzed transcriptional changes in soybean leaves on day 5 after P. syringae pv. syringae B728a infection. About 8,900 and 7,780 differentially expressed genes (DEGs) were identified in this study, and significant proportions of DEGs were engaged in various primary and secondary metabolisms. On the other hand, soybean orthologs to well-known plant immune-related genes, especially in plant hormone signal transduction, mitogen-activated protein kinase signaling, and plant-pathogen interaction, were mainly reduced in transcript levels at 5 days post inoculation. These findings present the feature of the compatible interaction between cultivar Kwangan and P. syringae pv. syringae B728a, as a hemibiotroph, at the late infection phase. Collectively, we propose that P. syringae pv. syringae B728a successfully inhibits plant immune response in susceptible plants and deregulates host metabolic processes for their colonization and proliferation, whereas host plants employ diverse metabolites to protect themselves against infection with the hemibiotrophic pathogen at the late infection phase.

• Journal of Microbiology and Biotechnology
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• 제24권4호
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• pp.563-567
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• 2014

Pseudomonas syringae pv. syringae (Psy) is a major bacterial pathogen of many economically important plant species. Despite the severity of its impact, the genome sequence of the type strain has not been reported. Here, we present the draft genome sequence of Psy ATCC 19310. Comparative genomic analysis revealed that Psy ATCC 19310 is closely related to Psy B728a. However, only a few type III effectors, which are key virulence factors, are shared by the two strains, indicating the possibility of host-pathogen specificity and genome dynamics, even under the pathovar level.

• 미생물학회지
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• 제55권1호
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• pp.80-82
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• 2019

Pseudomonas syringae pv. syringae WSPS007 균주는 대한민국 경상북도 영주시 사과 과원에서 나타난 가지마름병 병징으로부터 2013년 분리되었다. 본 논문에서는 6,238,498 bp(59.04% G+C 함량)인 WSPS007 균주의 전체 염기서열을 보고한다. 전체 지놈은 5,379개의 코딩서열, 65개의 tRNA, 16개의 rRNA 유전자를 가지고 있다. 특히 WSPS007 균주의 전체 염기서열 분석은 냉해와 관련된 빙핵 활성 유전자 클러스터를 중심으로 분석을 수행하였으며, P. syringae pv. syringae 국외 대표 균주인 PssB728a와 유사한 빙핵활성 유전자 구성을 가지고 있는 것으로 나타났다. 따라서 본 논문에서의 염기서열 분석 결과를 바탕으로 경상북도 일원 사과 과원에서 동해로 추정되는 병원균의 원인을 규명하기 위한 기본 자료를 제공하는데 있어서 의의가 있다고 사료된다.