The Plant Pathology Journal

The Korean Society of Plant Pathology (한국식물병리학회)
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RNA-seq Gene Profiling Reveals Transcriptional Changes in the Late Phase during Compatible Interaction between a Korean Soybean Cultivar (Glycine max cv. Kwangan) and Pseudomonas syringae pv. syringae B728a

  • Myoungsub, Kim (Department of Applied Bioscience, Dong-A University) ;
  • Dohui, Lee (Department of Applied Bioscience, Dong-A University) ;
  • Hyun Suk, Cho (Department of Applied Bioscience, Dong-A University) ;
  • Young-Soo, Chung (Department of Applied Bioscience, Dong-A University) ;
  • Hee Jin, Park (Department of Molecular Genetics, Dong-A University) ;
  • Ho Won, Jung (Institute of Agricultural Life Science, Dong-A University)
  • Received : 2022.08.25
  • Accepted : 2022.09.26
  • Published : 2022.12.01
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Abstract

Soybean (Glycine max (L) Merr.) provides plant-derived proteins, soy vegetable oils, and various beneficial metabolites to humans and livestock. The importance of soybean is highly underlined, especially when carbon-negative sustainable agriculture is noticeable. However, many diseases by pests and pathogens threaten sustainable soybean production. Therefore, understanding molecular interaction between diverse cultivated varieties and pathogens is essential to developing disease-resistant soybean plants. Here, we established a pathosystem of the Korean domestic cultivar Kwangan against Pseudomonas syringae pv. syringae B728a. This bacterial strain caused apparent disease symptoms and grew well in trifoliate leaves of soybean plants. To examine the disease susceptibility of the cultivar, we analyzed transcriptional changes in soybean leaves on day 5 after P. syringae pv. syringae B728a infection. About 8,900 and 7,780 differentially expressed genes (DEGs) were identified in this study, and significant proportions of DEGs were engaged in various primary and secondary metabolisms. On the other hand, soybean orthologs to well-known plant immune-related genes, especially in plant hormone signal transduction, mitogen-activated protein kinase signaling, and plant-pathogen interaction, were mainly reduced in transcript levels at 5 days post inoculation. These findings present the feature of the compatible interaction between cultivar Kwangan and P. syringae pv. syringae B728a, as a hemibiotroph, at the late infection phase. Collectively, we propose that P. syringae pv. syringae B728a successfully inhibits plant immune response in susceptible plants and deregulates host metabolic processes for their colonization and proliferation, whereas host plants employ diverse metabolites to protect themselves against infection with the hemibiotrophic pathogen at the late infection phase.

Keywords

Acknowledgement

This work was supported by the New Breeding Technologies Development Program of the Rural Development Administration (PJ01477702 and PJ01653304) (Ho Won Jung), the Basic Science Research Program through the National Research Foundation of Korea (NRF) funded by the Ministry of Education, Republic of Korea (2020R1A6A1A03047729) (Ho Won Jung and Hee Jin Park), and the Green Fusion Technology Graduate School Program of the Ministry of Environment (Myoungsub Kim, Dohui Lee).

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