• Journal of Microbiology
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• v.45 no.5
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• pp.402-408
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• 2007

A strain producing a potent protease was isolated from turban shell. The strain was identified as Bacillus sp. S17110 based on phylogenetic analysis. The enzyme was purified from culture supernatant of Bacillus sp. S17110 to homogeneity by ammonium sulfate precipitation, SP-Sepharose, and DEAE-Sepharose anion exchange chromatography. Protease activity of the purified protein against casein was found to be stable at pH 7 to pH 10 and around $50^{\circ}C$. Approximately 70% of proteolytic activity of the enzyme was detected either in the presence of 100 mM SDS or Tween 20. The enzyme activity was enhanced in the presence of $Ca^{2+},\;Zn^{2+},\;Mg^{2+}$, but was inhibited by EDTA, indicating that it requires metal for its activity. The purified enzyme was found to be a monomeric protein with a molecular mass of 75 kDa, as estimated by sodium dodecyl sulfate polyacrylamide gel electrophoresis (SDS-PAGE) and gel filtration chromatography. The purified enzyme was analyzed through peptide fingerprint mass spectra generated from matrix-assisted laser desorption ionization-time-of-flight-mass spectrometry (MALDI-TOF-MS) and a BLAST search, and identified as immune inhibitor A (inhA) deduced from nucleotide sequence of B. cereus G9241. Since InhA was identified as protease that cleave antibacterial proteins found in insect, inhA-like protease purified from Bacillus sp. S17110 might be pathogenic to sea invertebrates.

• Journal of Marine Life Science
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• v.7 no.2
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• pp.163-170
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• 2022

In this study, the results of hybridization were evaluated by analyzing the biomarkers of Haliotis discus hannai and hybrid abalone (H. madaka♀^*H. discus discus♂) in marine net cage. The survival rate was similar both experimental groups, but the growth (shell length) was about 10% faster in hybrid abalone. The deformity of respiratory pore in the hybrid abalone was about 6% lower than H. discus hannai, and the shell depression was about 15% lower in the hybrid abalone. In the biochemical composition, crude protein was about 3.1% higher in hybrid abalone, and showed similar values except for the crude protein. In the histological structure of the hepatopancreas, which performs the functions of digestion, absorption and detoxification of abalone, good results were obtained in the hybrid abalone. On these results, it is judged that the hybrid abalone will have high aquaculture productivity in the aquaculture environment.

• Journal of the Korean Society of Food Culture
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• v.21 no.6
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• pp.606-613
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• 2006

The present study examined the characteristic of regional food culture observed in prehistoric Daegok-ri petroglyph based on the history of art, archeological materials and existing whale eating customs. Daegok-가 petroglyph expressed animals in the sky, on the land and in the sea. According to the relic, the most preferred land and marin animals were deer and whale, respectively. In terms of petroglyphic techniques. earlier patch engraving was used mainly to express marin animal whale, and later line engraving to express land animal deer. This implies the possibility that as whale hunting declined due to regional environmental change the source of protein was switched to land animals. Among relics found in Dongsam-dong shell mound, which is a remain from the Neolithic Period, whale bones appear only in shell mounds of the Neolithic Period in coastal areas, and shell mounds until the Ironze Age, which were the agricultural Age, were found the bones of land animals such as deer and wild boar. This shows that in the petroglyph thematic land animals expressed in line engraving, which is a technique later than patch engraving, were major food resources for the supply of protein. Moreover, in terms of art, Daegok-ri petroglyph describes 'advanced hunters', suggesting that it was the Neolithic Period and showing 문 aspect of regional food culture based on hunting and fishing. The credibility, which prehistoric men expressed in Daegok-ri petroglyph before the invention of letters, was also proved by archeological articles.

• Korean Journal of Poultry Science
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• v.12 no.1
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• pp.39-44
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• 1985

These studies were conducted to compare the nutritive values and optimum prices of eggs among 6 groups of different egg Weight. With the total of 100 eggs of each weight group, after the weight percentage of egg yolk, albumen and shell in the whole egg were investigated, protein and fat contents of e99 yolk and albumen were analyzed. and then protein and fat contents in the whole eggs were calculated. Finally, the optimum prices of eggs in relation to the egg weight were studied on the basis of egg weight, protein content and protein plus fat contents of eggs, respectively. The results obtained are summarized as follows; 1. As the egg weight (X, g/10 eggs) increased, egg yolk (Y$_1$, %) and shell(Y$_2$, %) percentages tended to decrease, but egg albumen(Y$_3$, %) percentage increased lineally; Y$_1$=44.34-0.02X, Y$_2$=15.358-0.006 X, and Y$_3$=40.136＋0.026 X. 2. There were no significant differences in protein and fat contents of eggs among 6 different groups of egg weight. 3. Protein (Y$_1$, %), fat (Y$_2$, %) and protein plus fat (Y$_3$, %) contents in the whole eggs declined progressively as the egg weight (X, g/10 eggs) increased ; Y$_1$=11.943-0.00032X, Y$_2$=13.996-0.00614X, and Y$_3$=25.939-0.00646X. 4. Similar results were obtained whether the optimum prices of eggs were estimated on the basis of egg weight or protein content of eggs, and they were higher in the large size eggs and lower in the small size eggs than the optimum prices of eggs estimated on the basis of protein plus fat content of eggs.

• Journal of the Korean Applied Science and Technology
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• v.31 no.3
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• pp.387-393
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• 2014

In this study were performed to investigate the physicochemical properties of flying fish roe shell extract(FRSE) extracted from flying fish roe shell. FRSE was prepared using marine flying fish roe shell, and then analyzed it's physicochemical properties. The result showed that the nutritional composition of FRSE consisted of 81.00% protein, 9.12% ash and 4.48% moisture. There were OH-proline and glycine known as characteristics of collagen peptide in the amino acid analysis of FRSE, and there were large amount of glutamic acid and aspartic acid involved in the metabolism of glucose and fat. The calories of FRSE was 347 kcal/100g and molecular weight appeared less than 1,300 Da molecular weight distribution.

• Journal of the Korean Home Economics Association
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• v.20 no.3
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• pp.35-43
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• 1982

The purpose of this survey was to investigate the nutrient and food intake and haematology of the latter half of pregnant women in Nam Hae Do. The nutrient intake study was performed by Twenty-four hour dietary recall method. As Haematology, RBC, Hb. and Hct were measured. 1. The results of nutritional survey were, 1) The mean nutrient intakes that were below the RDA were Protein, Calorie, Calcium and Iron. 2) The mean nutrient intakes that were above the RDA were Vitamin A, Thiamin, Riboflavin, Ascorbic acid. 3) Most of calorie and other nutrients were obtained from vegetable food sources. 4) Animal protein intake was 33% of total protein intake and most of this value was obtained from fish and shell fishes. 2. The extent of malnutrition was explained in terms of the amount of calorie, protein, calcium and iron. The results were, The predicted percentage of deficiency, in case of Calorie, 53.3% of total subjects. in case of Protein, 52% of total subjects. in case of Calcium, 78.7% of total subjects. in case of Iron, 54.7% of total subjects. 3. The results of Haematology were, 1) The mean level of RBC, Hb and Hct were 3.76$\times$106cm/㎣, 10.47gm%, and 32.56% 2) There were significant correlation between calorie intake and Hct level, protein intake and RBC level, Iron intake and MCHC level.

• Journal of Physiology & Pathology in Korean Medicine
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• v.22 no.4
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• pp.878-883
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• 2008

Conchiolin is a complex protein which is secreted by the mollusc's outer epithelium to be the organic basis of mollusc shell. This study is to investigate a potential anti-inflammatory activity of conchiolin of oyster shell (COS). We tested the effects of COS on the lipopolysaccharide (LPS)-induced production of nitric oxide (NO) and prostaglandin E2 (PGE 2) in a murine macrophage cell line, RAW 264.7. COS inhibited production of NO and PGE2 in a dose dependent manner and also decreased the expression of inducible nitric oxide synthase (iNOS), cyclooxygenase-2 (COX-2), tumor necrosis $factor-{\alpha}$ ($TNF-{\alpha}$) and interleukin-6 (IL-6). These results suggest that COS can inhibit production of pro-inflammatory mediators and might be a useful source to treat inflammation.

• Journal of the Korean Ceramic Society
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• v.51 no.1
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• pp.7-10
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• 2014

Abalone shell is composed of 95 wt% $CaCO_3$ platelets and 5 wt% of a protein-rich organic matrix which acts as an adhesive layer, connecting aragonite tablets, thus maintaining the structural integrity of the composite. By mimicking abalone shell, we prepared a silicate plate/polymer nanocomposite by infiltrating PMMA between silicate layers and warm-pressing them at $200^{\circ}C$ for 1 h under 15 tons to make organic-inorganic composite materials. To examine the organic-inorganic composite materials after the warm-pressing procedure, the composite sample was analyzed with FE-SEM and TG. The bending strengths and densities of the composites prepared by a silicate plate and PMMA after the warm-pressing process were ~140 MPa and 1.5, respectively.

• Food Science of Animal Resources
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• v.32 no.3
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• pp.308-315
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• 2012

This study was conducted to examine the effects of egg shell membrane hydrolysates (ESMH) on skin whitening, wound healing, and UV-protection. ESMH was divided into three groups by molecular weight (Fraction I: above 10 kDa of ESMH, Fraction II: 3 kDa-10 kDa of ESMH, Fraction III: below 3 kDa of ESMH). As a result, all of ESMHs showed over 90% of protein contents. The wound healing experiment using HaCaT cells showed that the fraction I was slightly superior to other fractions depending on the concentration though it was not significantly different. In the experiments of inhibition of tyrosinase and L-3,4-dihydroxyphenylalanine (L-DOPA) oxidation to verify the L-DOPA whitening effect, the whole ESMH (before fractioning) showed a similar amount of inhibition effect with arbutin (control). In the inhibition of melanin formation in B16-F1 melanoma cells, the fraction I showed a high inhibitory effect. In the experiment for protecting the skin from ultraviolet rays using HaCaT cells, all the fractions showed a higher rate of cell viability than the control. In conclusion, this study confirmed that the cosmetic effects of ESMHs such as skin whitening, wound healing, and UV-protection, which were divided depending on the molecule weight. We could confirm that the possibility of ESMHs as a material for functional cosmetics.

• Korean Journal of Fisheries and Aquatic Sciences
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• v.43 no.5
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• pp.489-494
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• 2010

The nutritional demand of oyster larva (Crassostrea gigas) were investigated to determine the optimal culture conditions and improve micro-algae utilization. Changes in nutrients and shell growth were examined in fed and 96-h (48 h in late umbone stage) oysters at four larval stages. Shell growth increased significantly in D shape larvae, regardless of feeding variations. No growth was observed in starved larvae, except in shell length of umbone (to 11.9 ${\mu}m$). Fed larvae showed significant growth in all development stages (P < 0.05). During starvation, lipids were most significantly decreased in all larval stages (by 76.8%, 68.3%, 76.3%, and 40.3%, respectively), followed by protein (41.1%, 31.1%, 33.1%, 16.7%) and nitrogen-free extracts (40.8%, 24.3%, 36.9%, 20.1%), Gross energy (kcal/g) consumption in each larval stage was 49.6%, 35.1%, 39.1%, and 20.4%, respectively. Our results indicate that lipids are the most important energy source during the early larval development stages of C. gigas.