• 대한수의학회지
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• 제30권1호
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• pp.73-78
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• 1990

Hematological and blood chemical changes of rabbits infected with rabbit hemorrhagic disease virus were studied and the results were summarized as follows: 1. Total leukocyte count ($2,410{\pm}1,076/{\mu}l$), lymphocyte count ($1,582{\pm}632.5{\mu}l$) and heterophil count ($705{\pm}411.1{\mu}l$) were significantly decreased after 24 hours of the infection (p<0.01). However, no significant changes were observed in monocyte, eosinophil and basophil numbers. 2. A significant increase of aspartate aminotransferase (96IU/L), alanine aminotransferase (96IU/L) and alkaline phosphatase ($401.1{\pm}131.8IU/L$) was observed (p<0.01). 3. A moderate increase of BUN ($26.9{\pm}3.6mg/100ml$) and creatinine ($3.2{\pm}1.9mg/100ml$) was observed (p<0.05). 4. No significant changes of r-GTP, thymol turbidity, glucose, cholesterol, albumin, total serum protein, fibrinogen were observed.

• 한국임상수의학회지
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• 제8권1호
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• pp.11-26
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• 1991

The effects of intra-articula. sodium hyaluronate(SH) and polysulfated glycosaminoglycan(PSGAG) on degenerative joint disease of the carpus were compared each other In 20 racehorses. Ten horses were dosed with intra-articula. injection of 20mg SH(2 times/2 weeks interval) and ten horses were dosed with intra-articular injection of 250mg of PSGAG(4 times/1 week into.val). Synovial fluid analysis and clinical examination were made to evaluate the effects of the drugs on degenerative joint disease at before injection and 2 weeks after the last injection, respectively. Appearance and mucinous precipitate quality oi synovial fluids of the group injected with 58 and PSGAG were improved by 40～50% and 60～80%, respectively. The chemical values of alkaline phosphatase, lactic dehydrogenase, aspartate aminotransferase, total protein, A/G ratio and glucose of synovial fluid in the group injected with PSGAG were more clearly returned to the normal values than those of the group injected with SH. Relative viscosity and total white blood cells of synovial fluid were returned to the normal walues after the treatments in both groups. Clinical symptoms(swelling, heat and pain on carpal joint, and lameness) of the horses in the group injected with SH and PSGAG were disappeard by 56～67% and 67～80%, respectivelty. Conclusively, the PSGAG was superior to SH in the effects on treatment of the degenerative joint disease in the horses.

• Journal of Acupuncture Research
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• 제21권4호
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• pp.237-249
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• 2004

유근피는 혈액청정작용과 혈액순환에 영향을 주는 성분으로서 골 손상의 처방전으로 자주 사용된다. 현재까지 유근피가 골재형성에 미치는 영향은 약리학적으로 불확실하였다. 이에 저자들은 본 연구에서 유근피를 약침액으로 제조하여 유근피 약침액이 골세포에 미치는 영향을 in vitro에서 연구하였다. 방법으로 인체의 골아전구세포osteoprecursor cells (OPC-1)를 각각의 다른 유근피 농도를 함유한 매체내에서 부화시키고 그에 따른 세포증식을 연구하였으며, 유근피 약침액의 농도가 $100{\mu}g/ml$ 미만이었을 때 OPC-1의 증식량은 증가되었다. 그러나 농도가 $180{\mu}g/ml$을 초과하였을 때는 약물의 독성에 의해서 OPC-1의 증식량이 확연히 억제되었다. 대부분의 처치에서 세포들이 cyclooxygenase-2 (Cox 2) 단백질에 대해서 매우 명백한 발현을 보여줬다. 배양과정 중에 유근피 약침액 농도 최소치인 $1.0{\mu}g/ml$에서 최대치인 $500{\mu}g/ml$까지 경미하게 강화된 띠를 나타내었다. 이와 같은 실험의 결과로 볼 때 유근피 약침액은 골세포의 증식활동, alkaline phosphatse(ALP) 활동 및 total protein 분비의 증가와 골세포내에서의 농도의존적 약침액 투여량에 따른 OPC-1의 독특한 type I collagen 합성에 직접적인 억제작용을 주는 것을 관찰할 수 있으므로 추후 이와 유사한 실험을 통한 보다 발전적인 연구가 이루어져야 한다고 사료되었다.

• 한국임상수의학회지
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• 제8권2호
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• pp.157-170
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• 1991

Effects of recombinant bovine somatotropln(${\gamma}$BST) on hematologie and blood chemical values were investigated in twenty-five multiparous Holstein dairy cows. Recombinant BST was administered by two different routes ; intramusculary(12.5mg and 25mg/day) and subcutaneously(500mg and 750mg) in sustained-release vehicle every 2 weeks beginning 4 weeks postpartum and continuing for 7 months. Whole blood and serum samples were collected 0, 1, 2, 3, 5, and 7 months after beginning of treatments from control and ${\gamma}$BST-administered groups. Hematologic values including RBC, PCV, HB, MCH, MCHC, WBC and differential counts of treatment groups receiving ${\gamma}$BST were similiar to those of control group. Blood chemical values observed were total protein, albumin, A/G ratio, glucose, cholesterol, Ca, Pi, Ca/pi ratio, total bilirubin, creatinine, BUN, alkaline phosphatase, lactate dehydrogenase, alanine aminotransferase and aspartate aminotransferase. There were no significant differences in blood chemical values of cows administered with ${\gamma}$BST from those of control. Although some blood chemical values were fluctuated at a certain observation period, they were remained within the normal physiological ranges. It is concluded from the observations of these experiments that the dose and dosage froms of ${\gamma}$BST employed in this work might not affect hematologic and blood chemical values in dairy cows under the normal sanitary condition and adequate nutritional balance.

• Nutrition Research and Practice
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• 제10권5호
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• pp.507-515
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• 2016

BACKGROUND/OBJECTIVES: This study was designed to investigate whether Gynura procumbens extract (GPE) can improve insulin sensitivity and suppress hepatic glucose production in an animal model of type 2 diabetes. MATERIALS/METHODS: C57BL/Ksj-db/db mice were divided into 3 groups, a regular diet (control), GPE, and rosiglitazone groups (0.005 g/100 g diet) and fed for 6 weeks. RESULTS: Mice supplemented with GPE showed significantly lower blood levels of glucose and glycosylated hemoglobin than diabetic control mice. Glucose and insulin tolerance test also showed the positive effect of GPE on increasing insulin sensitivity. The homeostatic index of insulin resistance was significantly lower in mice supplemented with GPE than in the diabetic control mice. In the skeletal muscle, the expression of phosphorylated AMP-activated protein kinase, pAkt substrate of 160 kDa, and PM-glucose transporter type 4 increased in mice supplemented with GPE when compared to that of the diabetic control mice. GPE also decreased the expression of glucose-6-phosphatase and phosphoenolpyruvate carboxykinase in the liver. CONCLUSIONS: These findings demonstrate that GPE might improve insulin sensitivity and inhibit gluconeogenesis in the liver.

• The Korean Journal of Physiology
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• 제29권2호
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• pp.191-202
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• 1995

The purpose of this study was to compare effects of insulin and IGFs on growth, apical membrane enzyme activities and membrane transport systems of primary cultured rabbit kidney proximal tubule cells. Results were as follows: 1. Insulin and IGF-I produced significant growth stimulatory effects at $5{\times}10^{-10}M.\;IGF-II(5×10^{-10}\;M)$ did not stimulate significant cell growth. 2. Insulin stimulated the phosphorylation of a 97 KD protein. It was difficult to determine whether this band represents insulin and/or the IGF-I receptor. 3. The activities of apical membrane enzymes (alkaline phosphatase, leucine aminopeptidase, and ${\gamma}-glutamyl \;transpeptidase)$ were observed to be diminished after the cells were placed in the culture environment. 4. The uptake of ${\alpha}-MG,$ Pi and Na was significantly increased in cells incubated with insulin or IGF-I, IGF-II had no effect on the uptake of these substrates. 5. Na-pump activity, as assayed by Rb uptake, was significantly increased in cells treated with insulin or IGFs. In conclusion, insulin and IGF-I exert stimulatory effects on growth and membrane transporter(glucose, Na, Pi, and Na-pump) activities in primary cultured rabbit kidney proximal tubule cells. IGF-II had no effect on cell growth and membrane transporter(glucose, Na and Pi) activities.

• 한국임상수의학회지
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• 제6권1호
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• pp.209-216
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• 1989

In order to investigate sedative action of detomidine and its effect on physical signs, hematological and blood chemical components, 15 Holstein cattle were used. The dosage of detomidine was 25 ${\mu}$g/kg and 50 ${\mu}$g/kg. Blood was collected before injection, 30, 60 and 120 min. after injection. Induction time of sedation in a cattle given with 25 ${\mu}$g/kg and 50 ${\mu}$g/kg of detomidine was 10.6${\pm}$2.8. 7.6${\pm}$1.0min. respectively and maintenance time was 70.4${\pm}$8.3, 86.5${\pm}$9.9, respectively. After injection of detomidine, body temperature was slightly increased, heart rate and respiratory rate were slightly decreased. The levels of red blood cell, hemoglobin, packed cell volume and white blood cell were not changed by detomidine. Blood glucose level following detomidine was markedly increased but total protein, serum glutamic oxaloacetic transminase, alkaline phosphatase, lactic dehydrogenase, blood urea nitrogen and creatinine were not changed. This results indicated that detomidine was useful sedative in bovine practice.

• 한국환경보건학회지
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• 제29권2호
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• pp.23-28
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• 2003

To evaluate the effect of cyclohexane(CH) treatment on the liver cytochrome P-450 dependent aniline hydroxylase(CYPdAH) activity in alcohol-pretreated animals, CH(1.56 g/kg body weight) was intraperitoneally administered to Sprague-Dawley male rats, which had been drunk 15％ alcohol in distilled water for 1,3 and 6 weeks. CH was injected to rats 4 times every other day and the animals were sacrificed at 24 hours after injection of CH. In the alcohol-pretreated rats, liver injuries were not demonstrated on the basis of the liver weight per body weight, the levels of serum alanine aminotransferase and hepatic microsomal glucose-6-phosphatase activities. By the CH treatment, alcohol-pretreated animals showed the significantly increased activity of hepatic microsomal CYPdAH. Concomitantly $V_{max}$ value in CYPdAH was more increased, whereas $K_{M}$ value more decreased in alcohol-pretreated animals by the treatment of CH. In conclusion, the increasing cause of microsomal CYPdAH in CH-treated rats pretreated with alcohol may be due to induction of enzyme protein in rat liver.r.r.

• 대한의생명과학회지
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• 제15권4호
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• pp.287-293
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• 2009

Phospholipase D (PLD) is an enzyme hydrolyzing phosphatidylcholine to phosphatidic acid (PA) and choline. We investigated the involvement of PLD1 in the uptake of norepinephrine (NE) in PC12 cells, pheochromocytoma cells. NE uptake was specific in PC12 cells because nomifensine, a specific blocker of NE transporter, blocked NE uptake. Inhibition of PLD function in PC12 cells by the treatment of butanol suppressed the NE uptake. In contrast, overexpression of PLD1 in PC12 cells increased NE uptake efficiently. These results suggest that PLD activity is involved in NE uptake. We explored the action mechanism of PLD in NE uptake. PA phosphatase inhibitor, propranolol, blocks the formation of PKC activator diacylglycerol from PA. Propranolol treatment to PC12 cells blocked dramatically the uptake of NE. Specific PKC inhibitors, GF109203X and Ro31-8220, blocked NE uptake. Taken together, we suggest for the first time that PLD1 activity is involved in NE uptake via the activation of PKC.

• Food Science and Biotechnology
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• 제15권2호
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• pp.238-243
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• 2006

The methanol extract of waxy brown rice fermented with Agrocybe cylindracea was prepared. The extract was then freeze dried and fed to rats at the level of 0.5, 1.0, 2.0 g/kg body weight for 14 days, followed by the treatment with carbon tetrachloride for three consecutive days to induce hepatotoxicity. After sacrificing the rats, the enzyme activity of aspartate aminotransferase (AST), alanine aminotransferase (ALT), alkaline phosphatase (ALP), lactate dehydrogenase (LDH), and ${\gamma}$-glutamyl transpeptidase (${\gamma}$-GTP) in serum was determined. Biochemical analysis on serum for albumin, total protein, triglyceride, and total as well as HDL-cholesterol were carried out along with a histopathological study of liver tissues. Based on these data, we suggest that the waxy brown rice cultured with A. cylindracea may exert hepatoprotective activity against hepatotoxicity caused by chemicals such as carbon tetrachloride.