Biomedical Science Letters (대한의생명과학회지)

The Korean Society for Biomedical Laboratory Sciences (대한의생명과학회)
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Involvement of Phospholipase D in Norepinephrine Uptake in PC12 Cells

  • Rhee, Jong-Joo (Department of Neurosurgery, Cheongju St. Mary's Hospital) ;
  • Oh, Sae-Ock (Department of Anatomy, College of Medicine, Pusan National University) ;
  • Kim, Young-Rae (Research Institute for Medical Sciences and Department of Biochemistry, College of Medicine, Chungnam National University) ;
  • Park, Jong-Il (Research Institute for Medical Sciences and Department of Biochemistry, College of Medicine, Chungnam National University) ;
  • Park, Seung-Kiel (Research Institute for Medical Sciences and Department of Biochemistry, College of Medicine, Chungnam National University)
  • Published : 2009.12.31
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Abstract

Phospholipase D (PLD) is an enzyme hydrolyzing phosphatidylcholine to phosphatidic acid (PA) and choline. We investigated the involvement of PLD1 in the uptake of norepinephrine (NE) in PC12 cells, pheochromocytoma cells. NE uptake was specific in PC12 cells because nomifensine, a specific blocker of NE transporter, blocked NE uptake. Inhibition of PLD function in PC12 cells by the treatment of butanol suppressed the NE uptake. In contrast, overexpression of PLD1 in PC12 cells increased NE uptake efficiently. These results suggest that PLD activity is involved in NE uptake. We explored the action mechanism of PLD in NE uptake. PA phosphatase inhibitor, propranolol, blocks the formation of PKC activator diacylglycerol from PA. Propranolol treatment to PC12 cells blocked dramatically the uptake of NE. Specific PKC inhibitors, GF109203X and Ro31-8220, blocked NE uptake. Taken together, we suggest for the first time that PLD1 activity is involved in NE uptake via the activation of PKC.

Keywords

References

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