• 한국작물학회지
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• 제42권6호
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• pp.647-651
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• 1997

무피복 재배조건에서 생육특성 및 수량성 그리고 종실의 단백질과 지방함량, 지방산 조성을 조사하여 무피복 재배법 확립을 위한 기초자료를 얻고자 대광땅콩, 대풍땅콩을 공시하고 흑색 비닐 피복재배와 비교하여 시험한 결과는 다음과 같다. 1. 출현기는 대광땅콩, 대풍땅콩 모두 피복재배보다 6일정도 지연되었으며 개화기는 각각 9일, 6일 지연되었고 입모율도 각각 3.7％, 16％감소되었다. 2. 분지장은 피복재배보다 대광땅콩 24％(11.2cm), 대풍땅콩 17％(6.9cm) 감소되었으나 분지수는 감소되지 않았다. 3. 성숙협수,100립중, 협실비율, 주당 종실수량등은 피복재배보다 현저히 감소되었으나 밀식재배로 인한 재식밀도의 증가로 1ha당 종실수량은 대광땅콩의 경우 피복재배와 비슷하였고 대풍땅콩의 경우는 26％ 증수되었다. 4. 단백질함량은 대광땅콩의 경우, 피복재배보다 4％ 증가하였으나 대풍땅콩은 3％ 감소하였으며 지방함량은 두 품종 모두 각각 7％, 9％ 감소되었다. 5. 지방산 조성에서 oleic acid는 대광, 대풍땅콩 모두 피복재배보다 증가하였으나 linolelc acid는 감소하였으며 O/L비율도 작아졌다.

• 한국포장학회지
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• 제15권3호
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• pp.99-104
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• 2009

Soy protein isolate (SPI)-based nanocomposite films with three different types of nanoclays, such as Cloisite $Na^+$, Cloisite 20A, and Cloisite 30B, were prepared using a solution casting method, and their optical, tensile, and water vapor barrier properties were determined to investigate the effect of nano-clay type on film properties. Among the tested nanoclays, Cloisite $Na^+$, a hydrophilic montmorillonite (MMT), exhibited the highest transparency with least opaqueness, the highest tensile strength, and the highest water vapor barrier properties, indicating Cloisite $Na^+$ is the most compatible with SPI polymer matrix to form nanocomposite films. The film properties of SPI/Cloisite $Na^+$ nanocomposite films were strongly dependent on the concentration of the clay. Film properties such as optical, tensile, and water vapor barrier properties improved significantly (p<0.05) as the concentration of clay increased. However, the effectiveness of addition of the clay reduced above a certain level (i.e., 5wt%), indicating that there is an optimum amount of clay addition to exploit the full advantage of nanocmposite films.

• Biotechnology and Bioprocess Engineering:BBE
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• 제9권2호
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• pp.76-85
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• 2004

Biodevices composed of biomolecular layer have been developed in various fields such as medical diagnosis, pharmaceutical screening, electronic device, photonic device, environmental pollution detection device, and etc. The biomolecules such as protein, DNA and pigment, and cells have been used to construct the biodevices such as biomolecular diode, biostorage device, bioelectroluminescence device, protein chip, DNA chip, and cell chip. Substantial interest has focused upon thin film fabrication or the formation of biomaterials mono- or multi-layers on the solid surfaces to construct the biodevices. Based on the development of nanotechnology, nanoscale fabrication technology for biofilm has been emerged and applied to biodevices due to the various advantages such as high density immobilization and orientation control of immoblized biomolecules. This review described the nanoscale fabrication of biomolecular film and its application to bioelectronic devices and biochips.

• 산업식품공학
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• 제15권4호
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• pp.305-310
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• 2011

본 연구에서는 DSM을 이용하여 단백질 추출이나 정제없이 식품에 적용 가능성이 있는 가식성 필름을 개발하였다. HPH 처리는 표면이 매끄럽고 균일한 형태의 필름을 형성시켰고, 필름의 WVP값을 감소시켜 수분 방벽 효과를 높였다. 필름 내 글리세롤 농도 증가는 WVP값을 전반적으로 높였다. 농산물 가공 부산물인 DSM으로 제작된 가식성 필름은 새로운 식품 소재로서 상업적 적용 가능성을 보여주었다.

• Preventive Nutrition and Food Science
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• 제10권2호
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• pp.187-190
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• 2005

Silk fibroin (SF) films were prepared using various plasticizers and their physical properties were determined. Polyethylene glycol (PEG)-SF and polypropylene glycol (PPG)-SF films had tensile strengths (TS) of 23.71 MPa and 24.51 MPa, respectively, whereas the glycerol (G)-SF film had the lowest TS of 14.24 MPa. G-SF film had the highest $\%$ elongation, compared to PPG-SF and PEG-SF films. Water vapor permeability (WVP) of SF films varied with addition of plasticizers, and PEG-SF film had the lowest WVP. There was no significant difference in Hunter L value among treatments, but PEG-SF film had higher Hunter a and b values. These results suggest that SF film could be applied to food packaging and that the addition of plasticizers should improve the physical properties of SF film.

• Food Science and Biotechnology
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• 제16권1호
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• pp.62-66
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• 2007

Four different biopolyester films, two aliphatic polyesters including polylactides (PLA) and poly(3-hydroxy-butyrate-co-3-hydroxyvalerate (PHBV), and two aliphatic-aromatic copolyesters including Ecoplex and Biomax, were prepared using by thermo-compression, and their tensile and water barrier properties were determined. Among the films tested, PLA film was the most transparent (T: 95.8%), strongest, and stiffest (TS, 40.98 MPa; E, 1916 MPa), however it was rather brittle. In contrast, Ecoplex film was translucent while being the most flexible and resilient (EB, 766.8%). Biomax film was semitransparent and was the most brittle film tested (EB, 0.03%). All biopolyester films were water resistant exhibiting very low water solubility (WS) values ranging from 0.0.3 to 0.36%. PHBV film showed the lowest water vapor permeability (WVP) value ($1.26{\times}10^{-11}\;g{\cdot}m/m^2{\cdot}sec{\cdot}Pa$) followed by Biomax, PLA, and Ecoflex films, respectively. The water vapor barrier properties of each film were approximately 100 times higher than those of carbohydrate or protein-based films, but about 100 times lower than those of commodity polyolefin films such as low-density polyethylene (LDPE) or polypropylene (PP).

• 한국식품영양과학회지
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• 제20권2호
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• pp.133-138
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• 1991

생 표고버섯의 신선도 연장을 위한 연구로 두께(0.04mm-0.1mm)가 다른 PE 필름을 이용하여 표고버섯을 밀봉한 후 $2^{\circ}C$에서 60일간 저장하면서 품질변화를 조사하였다. 저장중 이취성분인 에틸알콜과 아세트알데히드 생성량은 PE 필름 두께에 비례하여 높은 값을 나타내었다. 중량 감소율은 저장 60일 경과후 0.06mm 밀봉구가 가장 낮았는데, 그 값은 같은 저장기간의 0.04mm 밀봉구의 중량감소율에 비해 1/2 정도의 낮은 값을 보였다. 한편 저장기간이 경과됨에 따라 표고버섯의 조단백질 함량이 증가하는 경향을 보였으며, 단백질의 전기영동 변화양상도 저장조건에 따라 그 차이가 뚜렷하였다.

• Biotechnology and Bioprocess Engineering:BBE
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• 제2권2호
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• pp.122-125
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• 1997

The utilization of excess whey is necessary to reduce dairy waste because the large amount of whey disposal in waste streams has caused environmental problems. During whey protein film production as the effective means of utilization of excess whey, we have examined the effects of pH, temperature, and plasticizers for water vapor permeability(WVP), tensile strength(TS), and elongation rate(%E) of the whey protein films. The 10% whey protein films had the highest WVP(28.73g$.$mm/kPa$.$day$.$㎡) and TS(1.85${\pm}$0.11Mpa). But, in this case, an increase of WVP was caused by the thickness of whey protein films. At the concentration of 8% whey protein, appropriate thickness was obtained. Whey protein films prepared at the pH 6.75 and 95$^{\circ}C$ showed lower WVP(28.38g$.$mm/kPa$.$day$.$㎡) and elongation rate(12.9%) and higher TS value(3.769${\pm}$0.407 MPa) than at the pH 6.75 and 75$^{\circ}C$. As the temperature increased, WVP of films decreased slightly and tensile strength increased slightly, while elongation rate decreased significantly. Higher WVP and TS were observed at pH6.75 compared to pH7-9. In contrast, significantly higher elongation was observed at pH 9comapred to pH6.75-8. Among the plasticizer types used, the addition of sorbitol showed the highest TS value(6.244${\pm}$0.297 MPa) at the concentration 0.4g sorbitol and elongation rate(49%) at the concentration of 0.6g sorbitol.

• 한국수산과학회지
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• 제35권4호
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• pp.417-423
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• 2002

명태 단백질을 이용하여 제조한 가식성 필름의 물성을 개선하기 위하여 필름 제조시 가소제 및 가교제 첨가, 그리고 이중필름에 의한 인장강도, 신장률 및 수분 투과도 등의 영향을 검토하였다. 가소제 첨가에 따른 가식성 필름의 인장강도는 PG, sorbitol, PEG 200 및 glycerol의 순으로 높았고, 신장률은 glycerol, sorbitol, PEG 200 및 PG의 순으로 높았으며, 수분투과도는 PG, sorbitol, glycerol 및 PEG 200의 순으로 낮았다. 또한 가소제의 농도가 증가함에 따라 필름의 인장강도는 감소하고 신장률은 증가하는 경향을 나타내었다. PG와 PEG 200은 신장률에 큰 영향을 미치지 못하여 명태단백질 필름의 가소제로 적절하지 못하였으며, 인장강도와 신장률이 서로 상반되는 glycerol과 sorbitol을 조합함으로써 필름의 물성을 조절할 수 있었다. 한편, 필름은 상대습도가 높을수록 인장강도는 감소하고 신장률과 평형수분함량은 증가하는 경향을 나타내었다. Ascorbic acid, citric acid 및 succinic acid 등의 가교제를 첨가하여 제조한 필름이 가교제 비첨가 필름에 비하여 인장강도와 수분투과도는 증가하고 신장률은 감소하는 경향을 나타내었으며, ascorbic acid는 $0.2%$, citric acid는 $0.1\%$, succinic acid 는 $0.1\%$ 첨가하는 것이 바람직하였다. 명태 단백질과 옥수수 단백질로 제조한 이중필름은 명태 단백질 단독으로 제조한 필름에 비하여 인장강도를 2배 이상 향상시켰고, 수분투과도를 약 $20\~30\%$ 감소시켰다. 한편, 산소투과도는 두 필름간에 차이는 없었으나, polyethylene film에 비하여는 10배 정도 산소차단효과가 우수하였다. 색도를 보면 명태와 옥수수 단백질을 이용하여 제조한 이중필름이 명태 단백질 단독으로 제조한 필름에 비하여 L값과 a값은 낮고 b값과 $\Delta$E값은 높았다

• Bulletin of the Korean Chemical Society
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• 제31권5호
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• pp.1223-1227
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• 2010

In this study, the orientational behavior of thermotropic liquid crystals (LC) supported on a film of protein receptors was examined. Avidin was roller printed and covalently immobilized onto the surface of gold using NHS/EDC chemistry. The orientation of nematic 4-cyano-4'-pentylbiphenyl (5CB) was found to be parallel to the plane of the printed avidin surface before incubation with a solution of biotin. However, protein-receptor complexation induced a random orientation of 5CB, where protein-receptor complexes disturbed the nanoscale topography of the printed protein surface. Atomic force microscopy and ellipsometry was used to confirm printing and the specific interaction of proteins. These results demonstrate that the combination of LC and roller printing can be used to detect specific interactions between biomolecules by manipulating the orientational behavior of LC to the printed protein surfaces.