• Journal of Aquaculture
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• v.6 no.1
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• pp.47-53
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• 1993

Feeding trials were conducted for a test of the optimum dietary protein levels for the fingerling Korean catfish, Parasilurus asotus. The growth response was examined in terms of weight gain, feed coefficient. protein efficiency ratio and net protein utilization for 8 weeks at $23\~27^{\circ}C$. Within a range of 25 to 55\%$ crude protein levels in the diet, the body weight increased while the dietary protein level increased. Accumulation of protein in the body reached the maximum when the crude protein level in the diet was at $45\%$. These results indicate that the optimum dietary protein level of fingerling Korean catfish was about $45\%$ when anchovy meal was used as the protein source.

• Journal of Nutrition and Health
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• v.19 no.1
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• pp.16-22
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• 1986

This study was designed to investigate the nutritional effect of mugowrt powder supple mentation to rice diets with different protein levels. Thirty female albino rats weighing 39-43g were adopted for the feeding trial for 4 weeks. The different 5 kind of experimental diets were performed . Control diet was commercially available forage for rats, experimental diet (I-C) highly milled rice, (Ⅰ) highly milled rice 95% and mugwort powder 5%, (Ⅱ-C)highly milled rice 95% and milk casein 5%, and (Ⅱ) highly milled rice 90%, milk casein 5 % and mugwort powder 5%. Growth rate was remarkably high in the dietary group fed on highly milled rice supplemented with 5% mugwort powder (protein 8%) (P<0.05), but it showed the tendency to be rather low in the group fed on highly milled rice supplemented with 5% casein and 5% mugwort powder (protein 12%). Food efficiency as well as protein efficiency appeared similar to the growth rate. Hematodcrit level demonstrated the same tendency as growth rate, but hemoglobin content was observed to increase by diets supplemented with increasing amount of mugwort regardless of protein level. Each nutrient intake was increased by adding mugwort powder to diets after a week's feeding, but it was increased by 8% protein diet, and decreased by 12% protein diet in 3 weeks as well as 4 weeks after feeding . The absorption rate of carbohydrate and protein decreased by feeding mugwort supplemented diets regardless of protein level and feeding period, and that of lipid increased with 12% protein diet.

• Korean Journal of Fisheries and Aquatic Sciences
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• v.37 no.1
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• pp.18-23
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• 2004

Effect of initial protein concentration on the protein removal rate was assessed for seawater aquarium using a foam separator. Protein removal rate was increased and removal efficiency was decreased with the increase of initial protein concentration. Enrichment ratio was decreased and foam generation rate was increased with the increase of initial protein concentration. Total suspended solids (TSS) removal rate was increased with the increase of initial protein concentration, and TSS removal efficiency was decreased with the increase of initial protein concentration. Turbidity removal rate and removal efficiency were increased with the increase of initial protein concentration.

• Korean journal of food and cookery science
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• v.23 no.6
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• pp.810-817
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• 2007

The quality properties of Jeung-pyun made with soybean protein isolate(SPI) were investigated. The SPI Jeung-pyun was manufactured using 3% whole protein, 7S protein or 11S protein(w/w). The redness values (a-values) of the Jeung-pyun were negative and the yellowness(b-value) of the control group was significantly lower compared to the other samples. The textural characteristics of the Jeung-pyun were influenced by the additions of SPI. The Jeung-pyuns containing soybean flour, whole protein, 7S and 11S protein had increases in springiness, but decreases in cohesiveness. In the sensory evaluation, overall desirability, sweetness, and moistness were highest in the whole protein group. The control group showed the highest scores for hardness and toughness, and the scores for after-taste and adhesiveness were the highest in the samples made with soybean flour. The 7S and 11S additions showed high scores in terms of color and springiness. In the analyses for consumer acceptance and sensory quality intensity, the whole protein addition had significantly higher scores for general acceptability and the Makgeolli flavor. Hardness was highest for the control group and the Makgeolli flavor was strongest with the soybean flour addition.

• Asian-Australasian Journal of Animal Sciences
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• v.1 no.1
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• pp.7-12
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• 1988

Twenty cows, by order of calving, were used in a completely randomized $2{\times}2$ factorial experiment. Variables were tow protein levels (14 and 18% crude protein) and concentration of fat (2 and 6% ether extract) in diets. Fat addition, via unprocessed whole sunflower seed, insured forage utilization in diets to meet energy requirement of cows. A total of 36 wks of lactation was subdivided into three 12-wk stages of lactation. Net energy lactation was set at 1.72, 1.57 and 1.42 Mcal/kg for each stage. Higher protein diets improved the efficiency of energy (FCM/net energy intake) which was particularly noted for diets containing high fat (85.7%). However, diets with low protein-high fat resulted in the lowest efficiency (67.7%). No difference in milk yield and butterfat was due to different levels and combinations of protein and lipid in diets. High protein diets depressed blood cholesterol and glucose compared to low-protein counterparts. Relative decline in milk production was slower for lower fat diets than for higher fat groups, especially mid to later stage of lactation. Results of this experiment tend to support our thesis on the synergistic effect of dietary protein and energy (lipid) upon efficiency of lactation.

• Asian-Australasian Journal of Animal Sciences
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• v.5 no.2
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• pp.365-368
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• 1992

The experiment was carried out to estimate the energy and protein requirements of Khaki Campbell $\times$ Thai Native laying ducks. To estimate the energy requirement, 5 experimental diets were formulated at levels of 2.70, 2.75, 2.80, 2.85 and 2.90 Mcal ME/kg. These diets were equal in ME/CP ratio (170/l). A total of 150 18-week old laying ducks were assigned to 5 energy level treatments, each comprising 3 replicates of 10 birds each, and they were fed the diets for 18 weeks. To estimate the protein requirement, 90 18-week old laying ducks were divided into 9 groups of 10 birds each, and they were assigned to 3 protein level treatments, each comprising 3 replicates. The levels of protein in the diets were 13.5%, 15.0% and 16.5%, and all diets were isocaloric (2.8 Mcal ME/kg). As a result, the 16.5% protein diet gave significantly better egg production than the 13.5% and 15.0% protein diets, however, no significant difference in egg production was found among the energy levels. Feed cost to produce 1k eggs was lower in the 2.70 Mcal and 16.5%protein diets than in the higher ME and lower protein diets.

• Journal of Life Science
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• v.8 no.2
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• pp.158-161
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• 1998

An inhibitor of farnesy-protein transferae is known to be a fgood candidate for antitumor agent that block the oncogenic activity of Ras protein . We recently isolated and characterized dihydrotanshinone I from Salvia miltiorrhiza Bunge (Danshen), an oriental herb, which has an inhibitory activity of toposimerase I to some cancer cell lines. In order to examine the molecular mechanism of dihydrotanshinone I, we studied the farmesy-Protein Transferase activity by dihydrotanshinone I. As a result, we found that result, we found that dihydrotanshinone I showed inhibitory effect on farnesyl-protein transferase with $IC_{50}$ value of 15 ug/ml. This result suggest that dihydrotanshinone I may be an useful anticancer agent with the inhibitory activity of farnesyl-protein transferase.

• Proceedings of the Korean Society for Bioinformatics Conference
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• 2005.09a
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• pp.313-318
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• 2005

Estimating the reliability of protein-protein interaction data sets obtained by high-throughput technologies such as yeast two-hybrid assays and mass spectrometry is of great importance. We develop a maximum likelihood estimation method that uses both protein localization and gene expression data to estimate the reliability of protein interaction data sets. By integrating protein localization data and gene expression data, we can obtain more accurate estimates of the reliability of various interaction data sets. We apply the method to protein physical interaction data sets and protein complex data sets. The reliability of the yeast two-hybrid interactions by Ito et al. (2001) is 27%, and that by Uetz et at.(2000) is 68%. The reliability of the protein complex data sets using tandem affinity purification-mass spec-trometry (TAP) by Gavin et at. (2002) is 45%, and that using high-throughput mass spectrometric protein complex identification (HMS-PCI) by Ho et al. (2002) is 20%. The method is general and can be applied to analyze any protein interaction data sets.

• Journal of Nutrition and Health
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• v.28 no.8
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• pp.782-790
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• 1995

The longitudinal changes in protein secretion from 27 lactating women(primiparae = 10, multiparae=17) and protein intake of infants have been studied from 0.5 to 6 months postpartum in Chungju and Anseong area. The protein contents o breast milk in primiparae appeared significantly higher than in multipaae at 0.5 and 1 month postpartum. The protein secretion of primiparae and multiparae was not significantly different. In breast-feeding period, there was a tendency that protein secretion from 0.5 to 2 months postpartum was higher than thereafter. Average protein intake of boys from milk from 0.5 to 6 months postpartum was significantly higher than that of girls(p<0.05), because volume of milk intake of boys was much more than that of girls. In the amount of protein intake per infant weight, there was no significant difference between boys and girls. Protein intakes per infant weight decreased during lactation. Mean energy consumption of lactating women was 2,327㎉/day, which was 93.1% of recommended energy allowance for Koreans. Mean dietary protein consumption of lactating women was 81g/day, which was 101.3% of recemmended protein allowance for Koreans. Energy ratio of carbohydrate : protein : lipid was appeared to 68.8 : 13.9 : 17.3.

• Proceedings of the Korean Society for Bioinformatics Conference
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• 2000.11a
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• pp.28-31
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• 2000

We describe a hidden Markov model, HMMTIR, for general protein sequence based on the I-sites library of sequence-structure motifs. Unlike the linear HMMs used to model individual protein families, HMMSTR has a highly branched topology and captures recurrent local features of protein sequences and structures that transcend protein family boundaries. The model extends the I-sites library by describing the adjacencies of different sequence-structure motifs as observed in the database, and achieves a great reduction in parameters by representing overlapping motifs in a much more compact form. The HMM attributes a considerably higher probability to coding sequence than does an equivalent dipeptide model, predicts secondary structure with an accuracy of 74.6% and backbone torsion angles better than any previously reported method, and predicts the structural context of beta strands and turns with an accuracy that should be useful for tertiary structure prediction. HMMSTR has been incorporated into a public, fully-automated protein structure prediction server.