• Fisheries and Aquatic Sciences
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• v.20 no.12
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• pp.31.1-31.11
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• 2017

Background: Liver-expressed antimicrobial peptide-2 (LEAP-2) is an important component of innate immune system in teleosts. In order to understand isoform-specific involvement and regulation of LEAP-2 genes in mud loach (Misgurnus mizolepis, Cypriniformes), a commercially important food fish, this study was aimed to characterize gene structure and expression characteristics of two paralog LEAP-2 isoforms. Results: Mud loach LEAP-2 isoforms (LEAP-2A and LEAP-2B) showed conserved features in the core structure of mature peptides characterized by four Cys residues to form two disulfide bonds. The two paralog isoforms represented a tripartite genomic organization, known as a common structure of vertebrate LEAP-2 genes. Bioinformatic analysis predicted various transcription factor binding motifs in the 5'-flanking regions of mud loach LEAP-2 genes with regard to development and immune response. Mud loach LEAP-2A and LEAP-2B isoforms exhibited different tissue expression patterns and were developmentally regulated. Both isoforms are rapidly modulated toward upregulation during bacterial challenge in an isoform and/or tissue-dependent fashion. Conclusion: Both LEAP-2 isoforms play protective roles not only in embryonic and larval development but also in early immune response to bacterial invasion in mud loach. The regulation pattern of the two isoform genes under basal and stimulated conditions would be isoform-specific, suggestive of a certain degree of functional divergence between isoforms in innate immune system in this species.

• YAKHAK HOEJI
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• v.52 no.1
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• pp.67-72
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• 2008

The use of doxorubicin, which is one of the most effective anticancer agents, is often limited by occurrence of acquired resistance in tumor cells. GSH has been shown to be involved in the development of this drug resistance. Transcription factor Nrf2 governs the expression of GSH synthesizing glutamylcysteine ligase (GCL), as well as multiple phase 2 detoxifying enzymes. Here we show that Nrf2 is one of factors determining doxorubicin sensitivity. Nrf2-deficient fibroblasts (murine embryonic fibroblasts, MEF) were more susceptible to doxorubicin mediated cell death than wild-type cells. Doxorubicin treatment elevated levels of Nrf2-regulated genes including NAD(P)H: quinone oxidoreductase (Nqo1) and GCL in wild-type fibroblasts, while no induction was observed in Nrf2-deficient cells. Doxorubicin resistance in human ovarian SK-OV cells was reversed by treatment with L-buthionine-sulfoxamine (BSO), which is depleting intracellular GSH. Finally, transfection of SK-OV cells with Nrf2 siRNA resulted in exacerbated cytotoxicity following doxorubicin treatment compared to scrambled RNA control. These results indicate that the Nrf2 pathway, which plays a protective role in normal cells, can be a potential target to control cancer cell resistance to anticancer agents.

• Journal of the Korea Safety Management & Science
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• v.18 no.1
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• pp.35-43
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• 2016

The past five years, industrial accidents due to food processing machinery have been occurred 725 cases, injury by the food cutter occurred 390 cases in its. On this study, to prevent food cutter injury, an attempt is made to present the improved documentation of fundamental safety of the food cutter workplace through the injury analysis of food cutter injury and surveys on band saw machine business field. Analyzing the result of 390 cases on food cutter injury, amputation, cut, puncture occupied 75.1 percent (293 cases), compressed occupied 23.3% (91 cases), also it showed constant component without reference to gender, age, scale of work place, service period. In the survey, lack of concentration for workers have been pointed out as the biggest factor in the cause of band saw machine injury. Meanwhile, such as the EU and Japan, whereas presents safety standards about band saw machines that are tailored to each country, on the other hand, South Korea doesn't provide the standards. To prevent the food cutter injury, safety standards need to be established in consideration of amputation, cut, puncture, compressed injury and financial support is required to procure protective equipment at each place of business.

• Tribology and Lubricants
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• v.16 no.4
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• pp.282-288
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• 2000

A sliding friction and wear test for silicon nitride (Si,N4) was conducted using a ball-on-disk specimen configuration. The material used in this study was HIPed silicon nitride. The tests were carried out from room temperature to 1000$^{\circ}C$ using self-mated silicon nitride couples in laboratory air. The worn surfaces were observed by SEM and the debris particles from the worn surfaces were analyzed for oxidation by XPS. The normal load was found to have a more significant influence on the friction coefficient of the silicon nitride than an elevated temperature. The specific wear rate was found to decrease along with the sliding distance. The specific wear rate at 29.4 N and 1000$^{\circ}C$ was 292 times larger than that at room temperature. The main wear mechanism from room temperature to 750$^{\circ}C$ was caused by brittle fracture whereas from 750$^{\circ}C$ to 1000$^{\circ}C$ the wear mechanism was mainly influenced by the oxidation of silicon nitride due to the increased temperature. The oxidation of silicon nitride at a high temperature was a significant factor in the wear increase.

• International Journal of Oral Biology
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• v.44 no.4
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• pp.182-190
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• 2019

Periodontitis is an inflammatory disease of the supportive tissues surrounding the teeth, and is characterized by irreversible destruction of the gingiva, periodontal ligament (PDL), and alveolar bone, which results in the loss of teeth. In the present study, we elucidated the correlation between periodontitis and apelin (APLN), an adipokine and a regulatory peptide, respectively, which are involved in inflammation and bone remodeling. The expression of APLN is negatively correlated with periodontitis progression in gingival tissue. In addition, treatment with TNF-α downregulated the expression of APLN in PDL cells and gingival fibroblasts, indicating the protective role played by APLN against periodontitis progression. The overexpression of APLN or treatment with exogenous APLN suppressed the TNF-α-mediated catabolic gene expression of MMP1, IL6, and PTGS2 in PDL cells. Moreover, the inhibition of the APLNA-PJ axis by ML221, an APJ inhibitor, induced catabolic gene expression in PDL cells. Thus, the results of this study provided evidence to support APLN as a regulatory factor of the inflammatory response during periodontitis.

• The Research Journal of the Costume Culture
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• v.16 no.1
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• pp.100-114
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• 2008

The purpose of this study was to analyze the characteristics of supermodern fashion designer's work, which is shown by responding to supermodern environment where non-spaces such as road, railroad and airport are surplus in modern city environment. For the study, the researcher used existing study and specialized book basically and analyzed photograph materials by selecting them in specialized fashion book, internet site and designer's homepage. The results of this study showed that supermodern fashion design is the functional fashion responding to the change of time and space concept of supermodern society directly. As the common points of supermodern designer's works, first, they try to create private space enabling wearer to cope with risk caused by the environmental change and transitional space by adding architectural factor to fashion. Second, they display the design with protective function and metaphorical expression of concealment and deception in the supermodern environment where nonspace is increasing. Third, designers search the functional design from the aspect of fabric and detail while intending the technology in order to cope with supermodern environment.

• Archives of Pharmacal Research
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• v.28 no.1
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• pp.81-86
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• 2005

The hepatoprotective effects of chalcone derivatives were evaluated in D-galactosamine/lipopolysaccharide (D-GaIN/LPS)-induced fulminant hepatic failure in mouse. Thirteen chalcone derivatives were synthesized for study and their hepatoprotective effects were evaluated by assessing aspartate aminotransferase (AST) and alanine aminotransferase (ALT) levels in serum. Chalcone preparations were injected into mice at 12 hand 1 h before intraperitoneal injection of D-GaIN/LPS. After abdominal administration, changes in AST and ALT between the control and treated groups were observed. Ten of the synthesized chalcone derivatives exhibited inhibitory effects on D-GaIN/LPS-induced levels of AST and ALT in mice. Compounds 2, 3, 8, 9, and 12 markedly reduced serum AST and ALT at 8 h, inhibited hepatocyte necrosis and showed significant hepatoprotective activities. The activity of compound 3 was compared with the bifendate (DDB) through oral administration. Compound 3 showed much higher inhibitory effects than bifendate for decreasing AST and ALT activity. The results indicate that compound 3 has strong hepatoprotective activity through suppression of tumor necrosis factor­alpha (TNF-alpha) preduction, reduction of the histological change in the liver, and attenuated of hepatocyte apoptosis confirmed by DNA fragmentation assay.

• Journal of the Korean Home Economics Association
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• v.51 no.2
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• pp.253-261
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• 2013

The purpose of this study was to investigate the moderating effects of parental monitoring on the relationship between children's dependency on mobile phones and control of learning behavior. The data came from the 2010 Korean Children and Youth Panel (N = 1,609) conducted by the National Youth Policy Institute. The analysis method used was Structural Equation Modeling by using SPSS 17.0 and AMOS 7.0. To test the significant moderating effects, Ping's two-step technique, which is free from the requirement of nonlinear constraints, was used. Our results demonstrated that children's dependency on mobile phones had negative effects on control of learning behavior, and the interaction effects between such dependency and parental monitoring affected the control of learning behavior. Thus, these results proved the moderating effects of parental monitoring in the control of learning behavior. This study suggests that parental monitoring buffers against having difficulties to control and adjust one's behavior associated with control of learning behavior, which is affected by the dependency on mobile phones among children. We discussed that the risks of children's dependency on mobile phones and parental monitoring should be acknowledge as a significant protective factor.

• Proceedings of the KIEE Conference
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• 2002.07a
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• pp.12-15
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• 2002

Korea Power System Protection Setting Rule was used from the rectify 1990's. Thereafter transmission voltage is raised the voltage into 765kV, and introduction to new technology of Power System, and was many of variation but, it is using. The present is using Digital type distance relay for 765kV transmission line protection. If impedance value of transmission line were to value lower than setting, this would be operating and relay setting rule is for 85% into Zone 1 self section, and Zone 2 is a 125%, Zone 3 is a 225%. Which's $15{\sim}25%$ include current transformer error 5%, potential transformer 5%, relay calculation error 5% and margin factor from the field experience. This paper is discussed transmission protective relay and relay setting rule of high voltage power system and we verify the correctness relay setting rule with distance relay using Matlab simulation.

• Proceedings of the KIEE Conference
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• 2002.07a
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• pp.229-231
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• 2002

The digital current differential relaying scheme is widely used for primary protection of 765(kV) power transformer. The current differential relay pickup the internal fault at the threshold which is set at 30% of rating current. Margin of 30% include current transformer error 5%, relay error 5%, on load tap changer error 7% and margin factor 140% obtained from the field experience. In this paper transformer protection relay and relay setting rule of high voltage power system are discussed. And we verify the correctness of relay setting rule with current differential relay using Matlab simulation.