• Reproductive and Developmental Biology
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• 제35권1호
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• pp.1-8
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• 2011

Techniques to evaluate gene expression profiling, such as sufficiently sensitive cDNA microarrays or real-time quantitative PCR, are efficient methods for monitoring human pluripotent stem cell (hESC/iPSC) cultures. However, most of these high-throughput tests have a limited use due to high cost, extended turn-around time, and the involvement of highly specialized technical expertise. Hence, there is an urgency of rapid, cost-effective, robust, yet sensitive method development for routine screening of hESCs/hiPSCs. A critical requirement in hESC/hiPSC cultures is to maintain a uniform undifferentiated state and to determine their differentiation capacity by showing the expression of gene markers representing all three germ layers, including ectoderm, mesoderm, and endoderm. To quantify the modulation of gene expression in hESCs/hiPSC during their propagation, expansion, and differentiation via embryoid body (EB) formation, we developed a simple, rapid, inexpensive, and definitive multimarker, semiquantitative multiplex RT-PCR platform technology. Among the 9 gene primers tested, 5 were pluripotent markers comprising set 1, and 3 lineage-specific markers were combined as set 2, respectively. We found that these 2 sets were not only effective in determining the relative differentiation in hESCs/hiPSCs, but were easily reproducible. In this study, we used the hES/hiPS cell lines to standardize the technique. This multiplex RT-PCR assay is flexible and, by selecting appropriate reporter genes, can be designed for characterization of different hESC/hiPSC lines during routine maintenance and directed differentiation.

• Journal of Korean Neurosurgical Society
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• 제37권3호
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• pp.217-222
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• 2005

Objective: To achieve optimal fit of implant, it is necessary to bend the implant during spine surgery. Bending procedure may decrease stiffness of plate especially made of titanium and stainless steel. Typically titanium suffers adverse effects including early crack propagation when it is bent. We investigate whether 6 degree bending of titanium plates would decrease the stiffness after full cyclic loading by comparing with non-bending titanium plates group. Methods: Authors experimented 40 titanium alloy plates of 57mm in length, manufactured by 5 different companies. Total 40 plates were divided into two groups (20 bent plates for experimental group and 20 non-bent plates for control group). Twenty plates of experimental group were bent to 6 degree with 3-point bending technique and verified with image analyzer. Using the electron microscope, we sought for a initial crack before and after 3-point bending. Mechanical testing by means of 6000 cyclic axial-compression loading of 35N in compression with moment arm of 35mm-1.1 Nm was conducted on each plate and followed by the electron microscopic examination to detect crack or fissure on plates. Results: The stiffness was decreased after 6000 cyclic loading, but there was no statistically significant difference in stiffness between experimental and control group. There was no evidence of change in grain structure on the electron microscopic magnification. Conclusion: The titanium cervical plates can be bent to 6 degree without any crack or weakness of plate. We also assume that minimal bending may increase the resistance to fatigue fracture in cervical flexion-extension movement.

• 한국지반공학회논문집
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• 제21권10호
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• pp.17-25
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• 2005

벤더엘리먼트 시험은 시험시편의 전단파속도를 직접 측정함으로써 최대전단탄성계수를 평가하기 위한 시험법으로, 다양한 실내시험장비에 간단히 부착되어 다양한 시험조건에서 시편의 최대전단탄성계수를 평가할 수 있는 유용한 시험기법으로 현재 널리 이용되고 있는 추세다. 본 연구에서는 포화가 가능하도록 개조된 Stokoe식 공진주/비틂전단 시험장비에 벤더엘리먼트 시험장비를 부착하여 동일한 시험시편에 대하여 다양한 조건에서 벤더엘리먼트 시험, 공진주 시험, 비틂전단 시험의 세 가지 시험을 동시에 수행함으로써 각 시험법에 의하여 평가되는 최대전단탄성계수를 비교하였으며, 벤더엘리먼트 시험기법을 검증해보고자 하였다. 또한 포화조건에서 Biot의 이론을 적용하여 각 시험기법의 하중주파수를 고려함으로써 보다 타당한 비교를 수행할 수 있었다.

• 한국전산유체공학회지
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• 제17권4호
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• pp.49-55
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• 2012

Research has importance in proposing the design of a tunnel with a vertical vent to secure passengers in a comfortable environment and safe against pressure. Using several analysis methods, the magnitude of the pressure induced by the vertical vent in the tunnel can be analyzed. In addition to the 3-dimensional method, the 2-dimensional method and the 2-dimensional axis-symmetric method are also used to analyze the strong and weak points of each so that the optimum analysis method can be obtained. As a result, it appears that the 2-dimensional axis-symmetric method is the most suitable in analyzing tunnel pressure consider to accuracy and time effective aspect. Also, the 3-dimensional method is disadvantageous in that it takes longer in calculating results, but is more effective in predicting phenomena around the vertical vent in the tunnel.

• International Journal of Aeronautical and Space Sciences
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• 제1권1호
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• pp.48-61
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• 2000

Korea Aerospace Research Institute (KARI) have gained experience with Helicopter Vibration Health Monitoring (VHM) System technology with the help of UK GKN-WHL. GKN-WHL have had many years of experience with the research and development of vibration analysis techniques to improve the health monitoring of helicopter transmissions. This activity was targeted at transmission rig testing at first, but the techniques have been progressively developed where they are now used as a part of integrated Health and Usage Monitoring (HUM) systems on many types of in-service and new helicopters. The technique development process has been considerably aided by an ever expanding database of transmission monitoring experience from both the rig testing and aircraft operations. This experience covers a wide range of failure types from naturally occurring faults to crack propagation studies and covering a wide range of transmission configurations. Primarily based on accelerometer signals GKN-WHL's vibration analysis methods have also been applied to a variety of other sensor types. The transition from an experimental environment to operational VHM systems has been a lengthy process, there being a need to demonstrate technique reliability as well as effectiveness to both regulatory (Airworthiness Authority) and commercial organizations. Another important feature of this process has been the development of close relationships with a number of VHM system hardware and software suppliers. Such an experienced GKN-WHL provides various raw vibration data which was acquired from transmission ground test rig and allow KARI to develop it's own analysis program. KARI made a program and then analyzed the data to coma pre with the results of GKN-WHL. The KARI's results both time domain signals and statistical values show comparable to GKN's.

• Asian-Australasian Journal of Animal Sciences
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• 제33권7호
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• pp.1113-1125
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• 2020

Objective: This study was investigated the effects of dietary supplementation of Antrodia cinnamomea fermented product on modulation of antioxidation, anti-inflammation, and lipid metabolism in broilers. Methods: Functional compounds and in vitro antioxidant capacity were detected in wheat bran (WB) solid-state fermented by Antrodia cinnamomea for 16 days (FAC). In animal experiment, 400 d-old broiler chickens were allotted into 5 groups fed control diet, and control diet replaced with 5% WB, 10% WB, 5% FAC, and 10% FAC respectively. Growth performance, intestinal microflora, serum antioxidant enzymes and fatty acid profiles in pectoral superficial muscle were measured. Results: Pretreatment with hot water extracted fermented product significantly reduced chicken peripheral blood mononuclear cells death induced by lipopolysaccharide and 2,2'-Azobis(2-amidinopropane) dihydrochloride. Birds received 5% and 10% FAC had higher weight gain than WB groups. Cecal coliform and lactic acid bacteria were diminished and increased respectively while diet replaced with FAC. For FAC supplemented groups, superoxide dismutase (SOD) activity increased at 35 days only, with catalase elevated at 21 and 35 day. Regarding serum lipid parameters, 10% FAC replacement significantly reduced triglyceride and low-density lipoprotein level in chickens. For fatty acid composition in pectoral superficial muscle of 35-d-old chickens, 5% and 10% FAC inclusion had birds with significantly lower saturated fatty acids as compared with 10% WB group. Birds on the 5% FAC diet had a higher degree of unsaturation, followed by 10% FAC, control, 5% WB, and 10% WB. Conclusion: In conclusion, desirable intestinal microflora in chickens obtaining FAC may be attributed to the functional metabolites detected in final fermented product. Moreover, antioxidant effects observed in FAC were plausibly exerted in terms of improved antioxidant enzymes activities, increased unsaturated degree of fatty acids in chicken muscle and better weight gain in FAC inclusion groups, indicating that FAC possesses promising favorable mechanisms worthy to be developed.

• 한국정보과학회논문지:데이타베이스
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• 제27권2호
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• pp.175-184
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• 2000

데이타 웨어하우스는 외부 데이타를 통합 요약하여 저장하는 시스템으로, 의사 결정에 필요한 정보를 효과적으로 제공할 수 있다. 대부분의 데이타 웨어하우스에서는 데이타를 정랴한 결과를 형성(materialized) 뷰의 형태로 저장한다. 이 때 뷰가 정의된 데이타 소스가 변화하면 뷰는 이를 반영하기 위 해 갱신되어야 한다. 뷰에 대한 갱신 작업은 상당한 부하를 야기시킬 수 있으므로, 이러한 갱신 작업을 효율적으로 수행하는 것은 매우 중요한 문제가 된다. 이미 뷰의 효율적인 갱신 방법에 대해서는 많은 연구 가 이루어져 왔다. 그러나 뷰가 여러 개의 데이타 소스에 의해 정의되고 이들 중 둘 이상의 데이타 소스가 변화된 경우, 이를 뷰에 반영하기 위해서 기존의 방법들은 데이타 소스에 대해 많은 수의 접근이 이루어져야 한다. 본 논문에서는 여러 개의 데이타 소스에 변화가 일어난 경우, 기존 연구에 비해 데이타 소스에 대한 접근을 줄일 수 있는 효율적인 뷰 갱신 방법을 제안한다 . 그리고 TPC-D 데이타를 사용한 실험을 통하여 본 논문에서 제안한 방법이 기존의 방법들보다 성능이 우수하다는 것을 보인다.

• 한국안전학회지
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• 제31권4호
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• pp.55-63
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• 2016

A dust explosion is a phenomenon of strong blast wave propagation involving destruction which results from dust pyrolysis and rapid oxidation in a confined space. There has been some research done to find individual explosion characteristics and common physical laws for various dust types. However, there has been insufficient number of studies related to the heat of combustion of materials and the oxygen consumption energy about materials in respect of dust explosion characteristics. The present study focuses on the relationship between dust explosion characteristics of wood dust samples and oxygen consumption energy. Since it is difficult to estimate the weight of suspended dust participating in explosions in dust explosion and mixtures are in fuel-rich conditions concentrations with equivalent ratios exceeding 1, methods for estimating explosion overpressure by applying oxygen consumption energy based on unit volume air at standard atmospheric pressure and temperature are proposed. In this study an oxygen consumption energy model for dust explosion is developed, and by applying this model to TNT equivalent model, initial explosion efficiency was calculated by comparing the results of standardized dust explosion experiments.

• 한국정보통신학회논문지
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• 제19권5호
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• pp.1259-1268
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• 2015

음향층심도의 변화에 대한 이해는 해양상층에서 수중음향 통신, 음향 토모그레피 및 수중항체를 수색하거나 탐지하는 해군 작전에 많은 영향을 미친다. 일반적으로 음향층심도와 혼합층깊이의 정의는 다르게 내려져있으나 혼합층 깊이가 음향학적으로는 음향층심도와 같다고 할 수 있다. 본 연구에서는 동해에서 가용한 수온-염분 관측자료를 활용하여 연간 음향층심도와 혼합층깊이를 비교해 보았다. 비교를 위해 다양한 혼합층깊이 결정 기준과 방법으로부터 도출된 혼합층깊이 자료를 활용하였다. 분석결과 동해에서 음향층심도는 곡률변화를 이용한 혼합층깊이와 거의 유사하였으나 다른 혼합층깊이 기준을 적용하였을 시는 많은 차이를 보였다. 한편 음향층심도 계산의 정확도를 평가해 보기 위해 음파전달손실 모의 실험을 실시하였다. 이를 통해 음향층심도내에서 음파전달손실이 최소가 되는 최적주파수가 존재함을 확인하였다.

• 한국위성정보통신학회논문지
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• 제2권2호
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• pp.1-9
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• 2007

본 논문은 고속철도 채널환경에서의 위성을 통한 Satellite Wibro 시스템의 성능열화특성과 이에 따른 보상기법에 대해 서술하였다. 고속철도 채널환경은 LOS채널 과 터널환경으로 구분되고 LOS 환경에서는 고속철도 파워 급전선에 의한 신호블로킹에 의한 문제와 터널내 에서는 Optic Fiber내에서와 같은 다중경로 간섭문제가 발생한다. 이러한 전형적인 두 채널 공간에서의 위성 와이브로 시스템 성능열화 특성을 해석적으로 규명하고 컴퓨터 시뮬레이션을 통하여 확인 하였다. 아울러 300 km/h 이상으로 이동하는 고속철도에서 발생되는 OFDM 시스템의 도플러 효과에 의한 ICI 현상과 이를 보상할 수 있는 기법을 해석적, 실험적 결과를 통하여 증명하였다.