• Korean Journal of Microbiology
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• v.39 no.2
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• pp.114-117
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• 2003

In molecular biology, it is necessary to develop an easy and rapid method to identify a specific DNA sequence. Though Southern and Northern blot techniques have been used widely for the analysis of gene structure and function, those methods are inconvenient in the points that we need to control incubation temperature, time, and other parameters to get the final result. In this study, we report a new method for the rapid analysis of specific DNA sequence with the modification of an immunochromatographic method. The lateral flow DNA analysis strip is composed of a sample pad, a nitrocellulose membrane for the separation and propagation of analytes, and an absorption pad for the generation of capillary action. Capture DNA was immobilized on the membrane by UV cross-linking and target DNA was labeled with Cy-5 for signaling. The samples containing target DNA were applied onto the sample pad, incubated for 15 min for separation, and scanned with a GSI fluorescence scanner. Though the hybridization reaction occurs in a short time without any washing steps, there appears to be little cross hybridization between the different sequences. The result showed a possibility that the new method can be used for the rapid identification of specific DNA sequence among the samples.

• The Journal of the Acoustical Society of Korea
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• v.11 no.2
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• pp.59-67
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• 1992

Recently, the problem which actively control the unwanted noise propagated from the technical structure by the generated secondary sound has become considerable topic from the environmental preservation point of view. In most of these studies, active noise control deals with a plane wave propagation at low frequency using adaptive filtering techniques. On the other hand, in real acoustic systems are mostly short due to the limitation of geometric configuration. In this case, the acoustic properties such as reflections and resonances inside the acoustic system should be considered. In this paper, the acoustic modeling method for short length duct was introduced using the transfer matrix method, and the active noise control problem was investigated with \implementation of FIR filter for the transfer function of control system derived from this modeling method. The identification methods for the acoustic model of actual control system was proposed by numerical computation technique based on the estimation of optimal FIR filter coefficients. The acceptable attenuation on the real acoustic system and stability of the controller are predicted in this computational simulation.

• Journal of the Institute of Electronics Engineers of Korea SP
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• v.37 no.1
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• pp.37-48
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• 2000

In this paper, we propose a new connected operator Using morphological grayscale reconstruction for region-based coding First, an effective method of reference-image creation lis proposed, which is based on the Size as well as the contrast. This improves the performance of simplification, because It preserves perceptually important components and removes unnecessary components The conventional connected operators are good for removing small regions, but have a serious drawback for low-contrast regions that are larger than the structuring element. That is, when the conventional connected operators are applied to tills region, the simplification becomes less effective or several meaningful regions are merged to one region to avoid this, the conventional geodesic dilation is modified to propose an adaptive operator to reduce the effect of inappropriate propagation, pixels reconstructed to the original values are excluded m the dilation operation Experimental results have shown that the proposed algorithm achieves better performance In terms of the reconstruction of flat zones. The Picture quality has also been improved by about 7dB, compared to the conventional methods.

• Journal of the Institute of Electronics and Information Engineers
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• v.50 no.1
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• pp.9-14
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• 2013

In this paper, we propose a low complexity bilateral search SIC for OFDM in fast time-varying channels. Due to the possibility of error propagation in SIC, symbol detection ordering within the block of symbols has a significant effect on the overall performance. In this paper, the first symbol to be detected is determined based on CSEP values, and then the next symbol to be detected is selected according to the updated CSEP while bilaterally searching from the boundary of the detected symbol group. Through computer simulations, we show that the proposed method has performance improvements with almost the same computation complexity over the conventional methods in the high SNR region. It has a performance approaching the MFB, known as the performance upper bound, within 2dB at the BER of $10^{-5}$.

• Korean Journal of Ichthyology
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• v.22 no.4
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• pp.217-224
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• 2010

An investigation was carried out to obtain basic information needed to develop methods for artificial propagation and conservation of the mottled skate Raja pulchra, an important food resource in western Korea that has declined from overfishing. In this paper we provide evidence of multiple spawning and describe properties of the ovary, and morphology of the egg capsule and the fully-formed offspring. The vitellogenic follicles in the ovary was $179.8{\pm}57.1$ (54~247) and can be classified into five size groups, which the last group of ova are considered as the moving to the capsule gland where fertilization and encapsulation of ova take place. The morphology of the egg capsule of R. pulchra is unique among the species of the family Rajidae and showed multi-embryo characteristics, having two to six yolks in each capsule. The adaptive morphological changes of larvae developing inside the egg capsule are described based on specimens extracted from the capsule.

• Clean Technology
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• v.23 no.1
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• pp.90-94
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• 2017

These days much efforts are being dedicated to wind power as a potential source of renewable energy. To maintain effective and uniform generation of energy, defect preservation of turbine blade is essential because it directly takes effects on the efficiency of power generation. For the effective maintenance, early measurements of blade defects are very important. However, current technologies such as ultrasonic waves and thermal imaging inspection methods are not suitable because of long inspection time and non-real time inspection. To supplement the problems, the study introduced a method for real time defect monitoring of a blade surface based on surface wave technology. We examined the effect of various parameters such as micro-cracks and peelings on the propagation of surface wave.

• Nuclear Engineering and Technology
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• v.52 no.4
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• pp.846-855
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• 2020

A loose part monitoring system is used to detect unexpected loose parts in a reactor coolant system in a nuclear power plant. It is still necessary to develop a new methodology for the localization and mass estimation of loose parts owing to the high estimation error of conventional methods. In addition, model-based diagnostics recently emphasized the importance of a model describing the behavior of a mechanical system or component. The purpose of this study is to propose a new localization and mass-estimation method based on finite element analysis (FEA) and optimization technique. First, an FEA model to simulate the propagation behavior of the bending wave generated by a metal sphere impact is validated by performing an impact test and a corresponding FEA and optimization for a downsized steam-generator structure. Second, a novel methodology based on FEA and optimization technique was proposed to estimate the impact location and mass of a loose part at the same time. The usefulness of the methodology was then validated through a series of FEAs and some blind tests. A new feature vector, the cross-correlation function, was also proposed to predict the impact location and mass of a loose part, and its usefulness was then validated. It is expected that the proposed methodology can be utilized in model-based diagnostics for the estimation of impact parameters such as the mass, velocity, and impact location of a loose part. In addition, the FEA-based model can be used to optimize the sensor position to improve the collected data quality in the site of nuclear power plants.

• The Journal of Korean Academy of Prosthodontics
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• v.46 no.3
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• pp.298-306
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• 2008

STATEMENT OF PROBLEM: Effect of surface treatment of ceramic under loading does not appear to have been investigated. PURPOSE: The aim of this study was to investigate the effect of surface treatment of esthetic ceramic, which is performed to increase the bonding strength, on the fracture stress under controlled cyclic loading condition. MATERIAL AND METHODS: Sixty 1.0 mm-thick specimens were made from Mark II Vitablocs (Vita Zahnfabrik, Germany) and divided into 3 groups: polished (control), sandblasted, and etched. Specimens of each group were bonded to a dentin analog material base including micro-channels to facilitate the flow of water to the bonding interface. Bonded ceramics were cyclically loaded with a flat-end piston in the water (500,000 cycles, 15Hz). Following completion of cyclic loading, specimens were examined for subsurface crack formation and subsequent stress was determined and loaded to next specimen by the staircase method according to the crack existence. RESULTS: There were significant differences of mean fatigue limit in the sandblasted (222.86 ${\pm}$ 23.42 N) and etched group (222.86 ${\pm}$ 14.16 N) when compared to polished group (251.43 ${\pm}$ 10.6 N) (P<.05; Wald-type pair-wise comparison and post hoc Bonferroni test). Of cracked specimens, surface treated group showed longer crack propagation after 24 hours. All failures originated from the radial cracking without cone crack. Fracture resistance of this study was very low and comparable to failure load in the oral cavity. CONCLUSION: Well controlled cyclic loading could induce clinically relevant cracks and fracture resistance of Mark II ceramic was relatively low applicable only to anterior restorations. Surface treatment of inner surface of feldspathic porcelain in the matsicatory area could influence lifetime of restorations.

• Journal of the Korea Concrete Institute
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• v.14 no.6
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• pp.922-933
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• 2002

Since the deterioration of concrete bridge decks affect durability, safety, and function, structural rehabilitation of damaged concrete deck that was strengthened with Fiber Reinforced Polymer(FRP) is increasing the latest. But recent studies on the strengthened structures are focused on the static behavior, however only a few studies on the fatigue behavior are performed. In this study, static and fatigue behavior of strengthened deck were peformed on 11 deck specimens strengthened with sheet typed Glass Fiber Reinforced Polymer(GFRP) that were reinforced by two different strengthening methods for the static test. A amount of strengthening material in the each direction such as transverse and longitudinal was adopted experimental variables for the static test and also the stress level of the static maximum load are adopted for the fatigue test. By the results of the experimental study, with respect to the strengthened decks, the resistance effect of crack propagation and effect of stress distribution are improved. In addition, the rate of variation of compliance decreased.

• The Journal of Information Systems
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• v.26 no.4
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• pp.419-442
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• 2017

Purpose The purpose of this study is to explore the pattern of user response and it's duration time through social media content response analysis. We also analyze the characteristics of content quality factors which are associate with the user response pattern. The analysis results will provide some implications to develop strategies and schematic plans for the operator of regional marketing on the SNS. Design/methodology/approach This study used mixed methods to verify the effects and responses of social media contents on the users who have concerns about regional events such as local festival, cultural events, and city tours etc. Big data analysis was conducted with the quantitative data from regional government SNSs. The data was collected through web crawling in order to analyze the social media contents. We especially analyzed the contents duration time and peak level time. This study also analyzed the characteristics of contents quality factors using expert evaluation data on the social media contents. Finally, we verify the relationship between the contents quality factors and user response types by cross correlation analysis. Findings According to the big data analysis, we could find some content life cycle which can be explained through empirical distribution with peak time pattern and left skewed long tail. The user response patterns are dependent on time and contents quality. In addition, this study confirms that the level of quality of social media content is closely relate to user interaction and response pattern. As a result of the contents response pattern analysis, it is necessary to develop high quality contents design strategy and content posting and propagation tactics. The SNS operators need to develop high quality contents using rich-media technology and active response contents that induce opinion leader on the SNS.