The function of the [4Fe-4S] cluster containing iron (Fe-) protein in nitrogenase catalysis is to serve as the nucleotide-dependent electron donor to the MoFe protein which contains the sites for substrate binding and reduction. The ability of the Fe protein to function in this manner is dependent on its ability to adopt the appropriate conformation for productive interaction with the MoFe protein and on its ability to change redox potentials to provide the driving force required for electron transfer. The MgADP-bound (or off) conformational state of the nitrogenase Fe protein structure described reveals mechanisms for long-range communication from the nucleotide-binding sites to control affinity of association with the MoFe protein component. Two pathways, termed switches I and II, appear to be integral to this nucleotide signal transduction mechanism. In addition, the structure of the MgADP bound Fe protein provides the basis for the changes in the biophysical properties of the [4Fe-4S] observed when Fe protein binds nucleotides. The structures of the nitrogenase Fe protein with defined amino acid substitutions in the nucleotide dependent signal transduction pathways of the Switch I and Switch II have been determined by X-ray diffraction methods. These two pathways have been also implicated by site directed mutagenesis studies, structural analysis and analogies to other proteins that utilize similar nucleotide dependent signal transduction pathways. We have examined the validity of the assignment of these pathways in linking the signals generated by MgATP binding and hydrolysis to macromolecular complex formation and intermolecular electron transfer. The results provide a structural basis for the observed biophysical and biochemical properties of the Fe protein variants and interactions within the nitrogenase Fe protein-MoFe protein complex.
Journal of the Korean Institute of Landscape Architecture
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v.40
no.4
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pp.62-77
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2012
This study aimed to investigate the Confucian-oriented agriculture phase of the Joseon Dynasty, which was reflected at the palaces, by analyzing constructional aspects and spatial characteristics of farmlands at the palace rear gardens. The objective sites were the rear gardens of Gyeongbok Palace, Changkyung Palace, and the outside of Sinmoomoon(神武門) This study was based on literature reviews. The farmlands at the palace rear garden were constructed to self-estimate the year's harvest condition within the palaces. It was a part of the agriculture encouragement policy on governing the group of Joseon like publishing the Nongsajiksul(農事直說) and establishing the Chingyeongnye(親耕禮: king's own cultivation ceremony) and Kikokje(祈穀祭: the rite of praying for grain). In addition, farmlands of the rear garden were operated from the beginning of the state almost until the Japanese colonial era. The results were summarized as follows: First, Gyeongbok Palace rear garden's farmland which begin at the reign of Sejong(世宗) existed at the present Hyangwonji(香遠池) area. It was constructed in order to check the advanced agricultural technologies. The rear garden's farmland in Changkyung Palace, which was executed during the reign of Seongjong(成宗), was constructed right after the initial Chingyeongnye of the Joseon Dynasty. Therefore, it might be understood as the context of the king's own cultivation of the Jeokjeonchinkyung(籍田親耕). Injo(仁祖) dug for farmland in the ground of the Gyemgdeok Palace(慶德宮) though there was some stay palace, when his stay dragged out for too long. This bespeaks that those farmlands at the palace rear gardens were of great importance in the Joseon political history. The farmland near Gyeongnongjae(耕農齋), which was made during the reign of Gojong(高宗), inherited predecessor's walks of the promoting agriculture and exhibited spatial compositions such as the rear garden's farmland at Changkyung Palace. Secondly, irrigation, its water systems, the name of observatory to study farming[觀耕臺] and location requirements for farmland had something in common. It was assumed as universal forms of physiocracy-space in the Joseon Dynasty. In this study, by considering aspects of operating about vegetable garden managed by eunuchs and of the orchard in palace to cultivate fruits for national ceremonies, it could be assumed that landscape architecture of royal palace in the Joseon Dynasty did not only focus on solemnity, orderliness and fanciness but also on the practical and productive which was helpful in life. In addition, the diverse activities of productive landscape architecture led by the royal family in palaces, and the initiatively tested advanced agricultural technologies by the king were considered as an aspect of the Korean traditional specific royal palace landscape architecture. That is considered sole landscape not only to love of the people but also the 'agriculture-first' principle which were absent from other nations.
Kim, Jin-Ho;Lee, Jong-Sik;Ryu, Jong-Su;Ahn, Eui-Young;Ahn, Seung-Gu
Korean Journal of Environmental Agriculture
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v.19
no.3
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pp.242-246
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2000
This study was carried out to show the chemical variations of monthly water quality and to estimate the quality as irrigation water at Seo-Lake. The water quality was surveyed at 6 sites for 7 months from May to November in 1999. The average concentration of $COD_{Cr}$ at Seo-Lake was 47.8mg/L. This value meant that the organic matters at the lake would exceed to the crop damage level (50mg/L). Especially, the values in May and in July were 83.07mg/L, 80.64mg/L, respectively. The average concentration of $NH_3-N$ was appeared to 2.84mg/L. But it was shown 5.72mg/L in May. That could put a restraint on the productive power of the crops. It was supposed that happened by eutrophication. And the water pollutant levels were high at stagnation part of the lake. But average water quality of the lake could be used for irrigation. The result showed that the research and the management of water quality were needed to make the lake water more suitable as the source for irrigation.
This study attempts to diagnose and categorize the characteristics of old industrial parks, and eventually link the results to the regeneration of industrial complexes. For this reason, we performed a factor analysis by utilizing 15 indices of 89 industrial parks, excluding 5 large equipment industry sites. The 15 indices were classified into 5 factors. Factor 1 can be described as a category of 'urbanization possibility' for the indices of building age, plot ratio of less than $1,650m^2$, and urbanization ratio of the surrounding area. Factor 2 can be described as a category of 'productive efficiency' for the indices of land productivity, amount of exports by land, employment productivity, and repair costs of industrial areas. Factor 3 can be described as a category of 'infrastructure amenity' for the indices of road ratio, plot ratio attached to the road, and parks and recreation ratio. Factor 4 can be described as a category of 'location potentiality' for the indices of land price, infrastructure age, and distance to the highway, while factor 5 can be described as a category of 'availability of supporting facilities' for the indices of parking lot ratio and supporting facility land ratio. By using these 5 factor scores, we were able to extract industrial parks included in the lower 25% of the factor score and searched for what kind of factor problem they have for each industrial park. Based on these results, this research will provide sufficient information on the decline of industrial parks with respect to their demerits. The results of this study show significant implications and contribute to the establishment of policies for regional competitiveness, as well as job creation, in the process of industrial regeneration.
Kim, Whee-Cheon;Hwang, Hae-Sung;Shin, Yong-Uk;Shin, Il-Sheob;Lee, Don-Kyun;Kang, Sang-Jo;Cheon, Byung-Deok;Moon, Jong-Youl;Kim, Jung-Ho
Horticultural Science & Technology
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v.19
no.1
/
pp.60-65
/
2001
'Shinil' pear cultivar (Pyrus pyrifolia Nakai) which was originated in a cross between 'Shinko' (non-patented, released in 1941) and 'Hosui' (non-patented, released in 1972) in 1978 was released as a middle season harvest variety. Its usual picking time coincided with 'Chuseok' season which is one of the most famous national holiday in Korea. The fruit showed high soluble solids content and good appearance. The cultivar was preliminarily selected in 1991, and its regional adaptability was evaluated in the name of 'Wonkyo Na-13' at 9 sites for four years from 1992, and finally selected and named in 1995. 'Shinil' is medium in tree vigor like 'Hosui' and spreading in tree habit as 'Niitaka', a leading cultivar in Korea, and consistently very productive. It has high resistance to black rot caused by Alternaria kikuchiana and pear necrotic spot caused by pear necrotic spot virus. Its full bloom is one day earlier than that of 'Niitaka' cultivar and harvest time is September 25 at Suwon area which is 3 days later than that of 'Hosui'. Fruit is round in shape with a deep medium stalk cavity and medium calyx basin and has attractive light yellow brown skin color. The fruit weight ranges between 300 and 400 g, which is similar to 'Chojuro', 'Shinko', and 'Hosui'. Soluble solid content is approximately at the level of 13-14 Brix, which is higher than that of 'Chojuro'. The flesh is cream-white, very juicy, and light grit with soft and fine texture.
In order to evaluate soil factors affecting the growth of Italian poplar, 23 areas planted with Italian poplar were surveyed. These 23 areas were classified into 3 categories, river-side, fallow-land and hill-side. The growth performance and soil factors for each area were investigated. The growth of Italian poplar at river-side was shown to be superior to that of fallow-land and fill-side. The rates of growth for fallow-land and hill-side are decreased by 8% and 21% compared to those of river-side, respectively. This suggests that plantation of Italian poplar at hill-side would not be profitable. Soil conditions of high productive area appeared liquid phase 20%, porosity 45%, water holding capacity 35 - 40%, soil hardness $1kg/cm^3$. pH 6 and rich in organic matter and total nitrogen. The results of factor analysis for soil factors affecting to Italian poplar growth that showed eigenvalue over 1 and communality value over 70% explained factor 1 : liquid phase, porosity and water holding capacity, factor 2 : pH and calcium, and factor 3 : soil hardness. This suggests that physical characteristics of soil is more important than chemical characteristics for Italian poplar growth. Multiregerssion analysis was conducted between diameter growth and soil hardness, liquid phase and calcium. The t-values for each independent variables showed significance at 1 - 10% level, but water holding capacity and pH are not significant. It is supposed that sites suitable to Italian poplar were alluvial plain of sandy loam or part of banking soil, well-ventilating soil, lower soil hardness, apposite soil moisture absorbing with about 100cm of ground water level, plentiful organic matters and total nitrogen and little acidity soil.
A green house experiment was conducted to find out the acetylene reducing and $N_2$-fixing activity from photosynthetic and aerobic heterotrophic nitrogen fixing microorganisms in submerged paddy soil under different agricultural locality, soil series, soil texture, soil type, and drainage condition in which samples taken from without nitrogen treatment plot of NPK trials on 16 sites of the farmer's field. The results obtained were summarized as follows: 1. The highest acetylene reducing activity was observed at 7 days after incubation in the light condition (photo synthetic microbes+heterotrophic bacteria) while it was observed at 35 days incubation in the dark condition (heterotrophic bacteria). 2. Among the soil series, photosynthetic nitrogen fixing activity was pronounced more in Jangae, Ogcheon and Hwadong series while lower was obtained in Buyong and Daejeong series. Aerobic heterotrophic nitrogen fixing activity was high in Buyong and Daejong series. 3. Estimated amount of $N_2$-fixation from acetylene reducing activity was equivalented to 3.0 mg in light condition and 4.9 mg/100g/105 days in dark condition. 4. Among the agricultural locality, photosynthetic nitrogen fixing activity was high in rather warm southern part while heterotrophic nitrogen fixing activity was predominated more in mountainous area and Chungcheong continental. 5. Photosynthetic nitrogen fixing activity was predominated in high productive soil while aerobic heterotrophic nitrogen fixing activity was pronounced more in crose coarse sandy soil. 6. The soils properties of high photosynthetic nitrogen fixing activity were constituted of poorly or imperfectly drained clay or clay loam soil while heterotrophic nitrogen fixing activity was pronounced more in well to moderately well drained sandy or sandy loam soil.
Journal of the Korea Institute of Building Construction
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v.22
no.5
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pp.473-483
/
2022
BIM(Building Information Modeling) is a technology that can manage information throughout the entire life cycle of the construction industry and serves as a platform for improving productivity and integrating the entire construction industry. Currently, BIM is actively applied in developed countries, and its use at various overseas construction sites is increasing This is unclear. due to air shortening and budget savings. However, there is still a lack of institutional basis and technical limitations in the domestic construction sector, which have led to the lack of utilization of BIM. Various activation measures and institutional frameworks will need to be established for the early establishment of these productive BIMs in Korea. Therefore, as part of the research for the domestic settlement and revitalization of BIM, this study derived a number of key factors necessary for the development of the construction industry through brainstorming and expert surveys using AHP techniques and analyzed the relative importance of each factor. In addition, prior surveys by a group of experts resulted in 1, 3 items in level, 2, 9 items in level, and 3, 27 items in level, and priorities analysis was performed through pairwise comparisons. As a result of the AHP analysis, it was found that the relative importance weight of policy aspects was highest in level 1, and the policy factors in level 2 and the cost-based and incentive system introduction factors were considered most important in level 3. These findings show that the importance of the policy guidance or institutions underlying the activation of BIM rather than research and development or corporate innovation is relatively high, and that the preparation of policy plans by public institutions should be the first priority. Therefore, it is considered that the development of a policy system or guideline must be prioritized before it can be advanced to the next activation stage. The use of BIM technologies will not only contribute to improving the productivity of the construction industry, but also to the overall development of the industry and the growth of the construction industry. It is expected that the results of this study can provide as useful information when establishing policies for activating BIM in central government, relevant local governments, and related public institutions.
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