• 한국육종학회지
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• 제43권4호
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• pp.311-317
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• 2011

이 실험은 다양한 크기의 종자로부터 콩나물을 키우고 콩나물의 생장 및 물리적 특성으로 조사함으로써 나물콩 품종육성과 나물콩 품질평가를 위한 기초자료를 얻고자 수행되었다. 대립 및 중소립 재배종 각각 3품종, 야생종과 재배종 교잡종 소립 3품종, 극소립 야생콩 3계통을 공시하여 4일간 콩나물을 키운 뒤 생육특성과 콩나물의 물성을 조사하였다. 콩나물 재배 96시간 후 배축 길이는 품종간 차이가 컸는데 황금콩, 풍산나물콩 및 소영이 가장 길었으며 극소립인 야생콩 계통들이 짧았다. 특히 대립종인 황금콩은 72시간까지 소립 나물콩 품종들과 비슷하게 생장하였으며 96시간 후에는 15.9 cm로 가장 길었다. 극소립 야생종계통은 재배 48시간 후부터 72시간 사이에 급격하게 증가하였으며 원료콩 1 g당 생장량도 월등히 많았으나 다른 품종들은 비슷한 증가양상을 보였다. 재배 96시간 후 단위 종자당 콩나물 생체중 증가비율은 소립일수록 컸으며 콩나물 수율도 소립종일수록 높은 경향을 보였다. 생체 배축의 hardness는 장엽콩이 3,505 g으로 가장 컸고 야생종 YWS516이 1,750 g으로 가장 낮았다. Mastication는 태광콩이 1,650 g으로 가장 높았으며 YWS516이 1,079g으로 가장 낮았으며 배축의 cutting force는 은하콩이 133 g으로 가장 크고 YWS516이 51 g으로 가장 낮았다. 익힌 후의 cutting force는 모든 품종에서 증가하였는데 전체적으로 종자크기가 클수록 물성특성이 증가하는 것으로 나타났다. 배축의 breaking force는 장엽콩이 83.5 g로 가장 컸고 야생콩이 가장 낮아 종자크기가 클 수록 증가하였다.

• Journal of Animal Science and Technology
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• 제60권12호
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• pp.32.1-32.10
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• 2018

Background: Necdin (NDN), a member of the melanoma antigen family showing imprinted pattern of expression, has been implicated as causing Prader-Willi symptoms, and known to participate in cellular growth, cellular migration and differentiation. The region where NDN is located has been associated to QTLs affecting reproduction and early growth in cattle, but location and functional analysis of the molecular mechanisms have not been established. Methods: Here we report the sequence variation of the entire coding sequence from 72 samples of cattle, yak, buffalo, goat and sheep, and discuss its variation in Bovidae. Median-joining network analysis was used to analyze the variation found in the species. Synonymous and non-synonymous substitution rates were determined for the analysis of all the polymorphic sites. Phylogenetic analysis were carried out among the species of Bovidae to reconstruct their relationships. Results: From the phylogenetic analysis with the consensus sequences of the studied Bovidae species, we found that only 11 of the 26 nucleotide changes that differentiate them produced amino acid changes. All the SNPs found in the cattle breeds were novel and showed similar percentages of nucleotides with non-synonymous substitutions at the N-terminal, MHD and C-terminal (12.3, 12.8 and 12.5%, respectively), and were much higher than the percentage of synonymous substitutions (2.5, 2.6 and 4.9%, respectively). Three mutations in cattle and one in sheep, detected in heterozygous individuals were predicted to be deleterious. Additionally, the analysis of the biochemical characteristics in the most common form of the proteins in each species show very little difference in molecular weight, pI, net charge, instability index, aliphatic index and GRAVY (Table 4) in the Bovidae species, except for sheep, which had a higher molecular weight, instability index and GRAVY. Conclusions: There is sufficient variation in this gene within and among the studied species, and because NDN carry key functions in the organism, it can have effects in economically important traits in the production of these species. NDN sequence is phylogenetically informative in this group, thus we propose this gene as a phylogenetic marker to study the evolution and conservation in Bovidae.

• 한국작물학회지
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• 제64권2호
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• pp.102-108
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• 2019

본 연구는 식용옥수수 풋이삭의 식감의 결정에 영향을 미치는 과피의 두께를 측정하는 방법을 규명하기 위하여 과피 시료의 준비 방법에 따른 과피 두께의 차이와 등숙 시기별 과피 두께의 변화를 조사하기 위하여 수행하였다. 1. 찰옥수수와 초당옥수수 각 3품종의 시료를 수확 직후 생옥수수(생시료) 알곡을 바로 측정하거나 상온의 음지에서 완전(건조시료) 건조시키거나 $-4^{\circ}C$로 냉동시킨 후 과피를 측정하였다. 2. 시료 준비 방법과 등숙 시기에 따른 삼원 교호작용의 유의성이 인정되어 어느 한 요인에 대한 일반적인 결론을 도출하기 보다는 각 시료 준비 방법과 특정 등숙 시기에 따른 과피 두께의 비교를 수행하였다. 3. 초당옥수수는 건조시료에서 등숙이 진행됨에 따라 비교적 안정된 과피 두께 측정값을 얻을 수 있었으며 찰옥수수는 비해 모든 시료 준비 방법에서 초당옥수수보다 안정된 값을 보였다. 특히 풋찰옥수수 수확 적기인 수정 후 24일경에는 시료 준비 방법에 따른 차이는 크게 나타나지 않을 것으로 기대된다.

• Journal of Animal Science and Technology
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• 제61권4호
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• pp.192-203
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• 2019

A study was conducted to determine the influence of feed form and microbial enzyme supplementation on energy utilization, bone quality, and amino acid and mineral digestibility of broiler chickens. Four hundred and eighty Ross 308, day-old broiler chickens were randomly assigned to eight diets formulated from commonly used ingredients in Tanzania. A 2 (pellet or mash) ${\times}$ 4 (control, Axtra XB, Quantum Blue (QB) and Axtra XB + QB enzyme) factorial array in a completely randomized design having six replicates per treatment (10 birds per replicate) was used. Birds were raised in climate-controlled rooms in a 3-phase; starter (0-10 days), grower (11-24 days) and finisher (25-35 days). Apparent metabolizable energy (AME), metabolizable energy intake, net energy of production, energy retained as protein (REp), and efficiency of metabolizable energy use for energy and protein retention were higher (p < 0.05) in birds fed pelleted diets. The AME and REp was higher (p < 0.05) with enzyme supplementation. Ash content, weight, length, width and breaking strength of tibia bones were highest (p < 0.05) in birds on pelleted diets. Tibia bone traits were improved (p < 0.05) when enzymes were included, particularly in a combination of QB and Axtra XB. However, potassium, magnesium, and zinc contents were highest (p < 0.05) when QB was supplemented. Digestibility of all amino acids was higher (p < 0.05) in birds supplied with pellets and with enzyme supplementation for most amino acids, except for serine. There was a positive interaction (p < 0.05) between feed form and enzymes on lysine and phenylalanine digestibility. Digestibility of Ca, P, K, S, Zn, and Fe was higher (p < 0.05) in birds fed pelleted diets, while those on mashed diets had higher (p < 0.05) digestibility of Cu and B. The digestibility of P, K, and Zn was highest (p < 0.001) when QB was added, while Ca, P, S, and B digestibility was highest when a combination of Axtra XB + QB was applied. Pelleted diets with or without enzymes improved energy utilization, digestibility of amino acids, and minerals, and increased bone strength in broiler chickens.

• Asian-Australasian Journal of Animal Sciences
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• 제32권9호
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• pp.1340-1348
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• 2019

Objective: The objectives were to compare variance components, genetic parameters, prediction accuracies, and genomic-polygenic estimated breeding value (EBV) rankings for milk yield (MY) and fat yield (FY) in the Thai multibreed dairy population using five single nucleotide polymorphism (SNP) sets from GeneSeek GGP80K chip. Methods: The dataset contained monthly MY and FY of 8,361 first-lactation cows from 810 farms. Variance components, genetic parameters, and EBV for five SNP sets from the GeneSeek GGP80K chip were obtained using a 2-trait single-step average-information restricted maximum likelihood procedure. The SNP sets were the complete SNP set (all available SNP; SNP100), top 75% set (SNP75), top 50% set (SNP50), top 25% set (SNP25), and top 5% set (SNP5). The 2-trait models included herd-year-season, heterozygosity and age at first calving as fixed effects, and animal additive genetic and residual as random effects. Results: The estimates of additive genetic variances for MY and FY from SNP subsets were mostly higher than those of the complete set. The SNP25 MY and FY heritability estimates (0.276 and 0.183) were higher than those from SNP75 (0.265 and 0.168), SNP50 (0.275 and 0.179), SNP5 (0.231 and 0.169), and SNP100 (0.251and 0.159). The SNP25 EBV accuracies for MY and FY (39.76% and 33.82%) were higher than for SNP75 (35.01% and 32.60%), SNP50 (39.64% and 33.38%), SNP5 (38.61% and 29.70%), and SNP100 (34.43% and 31.61%). All rank correlations between SNP100 and SNP subsets were above 0.98 for both traits, except for SNP100 and SNP5 (0.93 for MY; 0.92 for FY). Conclusion: The high SNP25 estimates of genetic variances, heritabilities, EBV accuracies, and rank correlations between SNP100 and SNP25 for MY and FY indicated that genotyping animals with SNP25 dedicated chip would be a suitable to maintain genotyping costs low while speeding up genetic progress for MY and FY in the Thai dairy population.

• Journal of Ecology and Environment
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• 제43권3호
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• pp.305-313
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• 2019

Background: This research aims to study the effect of climate change on the phenology, growth, and physiological traits of Silene capitata Kom., a Korean endangered species II. This study increased $CO_2$ concentration in a closed glass greenhouse, with the daily mean temperature and $CO_2$ concentration respectively being $4.61^{\circ}C$ and 93.63 ppm higher than the outside temperature (ambient conditions, control). The seeds of S. capitata were sown in control and treatment environments in March 2013 while seedlings were transplanted into individual pots in May 2013. To research phenological changes, the first day of the flowering and ripening of the plants transplanted in 2013 and first day of leafing in 2014 were observed. The growth and physiological responses of mature leaves were also studied in 2013. Results: There was no difference in the first day of flowering, but the first day of ripening was earlier in the treatment group than the control group. There was no difference in the number of rosette leaves between the two groups, but leaf area was wider in the treatment group than the control group. Transpiration rate and stomatal conductance were higher in the treatment group than the control group, chlorophyll content decreased, and photosynthetic rate and water use efficiency were the same for both groups. As a result of simple regression analysis among the transpiration rate, stomatal conductance, photosynthetic rate, and water use efficiency, stomatal conductance increased when transpiration rate increased. Stomatal conductance increased with photosynthetic rate in the control unlike in the treatment group. The photosynthetic rate and water use efficiency increased with transpiration rate in the control group unlike in the treatment group. Furthermore, water use efficiency increased as photosynthetic rate increased in both groups. Conclusion: Due to high $CO_2$ concentration, the photosynthetic rate was no longer controlled by the stomata, which appeared to suppress the excessive production of photosynthetic products by reducing chlorophyll content. It is believed that the phenological responses of S. capitata under climate change conditions will advance and that stable growth will be difficult in regions lacking moisture due to the high transpiration rate.

• 식물병연구
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• 제25권3호
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• pp.136-142
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• 2019

식물 근권과 내생에서 분리한 447 균주 중 식물 생장 촉진특성과 건조 내성이 있는 28 균주를 일차적으로 스크리닝 하였으며, PEG에 의한 인위적 건조 스트레스 조건에서 잎의 상대수분함량과 MDA를 기반으로 GLC02와 KJ40을 선발하였다. 이 두 균주의 효과를 검증하기 위해 밭흙을 사용한 자연 건조에서 식물 포트 검정을 하였으며, 기공전도도와 지상부(줄기와 잎) 무게가 유의하게 증가한 반면 MDA가 감소하였다. 병 억제 효과에서는 GLC02를 처리할 경우 역병의 병진전도가 감소하였으며, KJ40을 처리할 경우 세균성 점무늬병에 대한 억제효과가 있었다. 이를 토대로, GLC02와 KJ40을 처리할 경우 건조 스트레스를 경감시켜주며 식물생장의 증진, 병 억제효과를 유도하여 생물비료의 소재로 사용할 수 있을 것으로 생각된다.

• 기록학연구
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• 제53호
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• pp.79-117
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• 2017

개인 기록은 "기록"으로서의 일반적 특성과 "개인"이라는 맥락이 부여한 고유한 특성을 공유하고 있다. 이 글은 그 중에서도 고유한 특성에 관한 주목이 필요하며 개인 기록 관리에 관한 다양한 방법들을 결정하기 위해서는 개인 기록이 갖는 고유한 특성을 구체적으로 고찰해야 한다는 문제 의식에서 시작되었다. 이에 이 글은 개인 기록이 갖는 특성을 밝히기 위하여 두 가지 방법의 접근을 시도하였다. 먼저, 기록관리의 가장 기본적인 원칙을 담고 있는 표준인 ISO 15489: 2016와 개인 기록과 관련된 문헌 연구들을 중심으로 개인 기록의 생산 맥락, 기록관리 과정에서 살펴볼 수 있는 개인 기록의 고유한 특성을 살펴보았다. 다음으로 사람의 독특한 성질인 "성격(personality)"과 관련된 이론을 검토하고 개인 기록 관리에서의 적용 필요성을 검토했다. 아울러 국내의 기록 관리기관이 소장하고 있는 실제 개인에 관한 기록 군에서 성격을 중심으로 개인적 특성을 살펴보고 이것이 기록 관리에 어떤 영향을 주고 있는지를 확인해 보았다. 개인 기록이 갖는 고유한 특성이야말로 기록화 활동에 있어 수집대상에 대한 이해와 전략의 기반이 되어야 한다고 생각된다. 이를 위해서는 개인 기록이 가진 특성을 다양한 차원에서 발견하기 위한 노력이 우선적으로 필요하며 이를 개인 기록을 기록화하기 위한 전략의 핵심으로서 확보해 나가야 할 것이다.

• Asian-Australasian Journal of Animal Sciences
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• 제32권7호
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• pp.949-955
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• 2019

Objective: The present study was to investigate the association of polymorphisms in exon-9 of the bone morphogenetic protein receptor-1B (BMPR-1B) gene (C864T) with litter size in 240 Dorset, 232 Mongolian, and 124 Small Tail Han ewes. Methods: Blood samples were collected from 596 ewes and genomic DNA was extracted using the phenol: chloroform extraction method. The 304-bp amplified polymerase chain reaction product was analyzed for polymorphism by single-strand conformation polymorphism method. The genotypic frequency and allele frequency of BMPR-1B gene exon-9 were computed after sequence alignment. The ${\chi}^2$ independence test was used to analyze the association of genotypic frequency and litter size traits with in each ewe breed, where the phenotype was directly treated as category. Results: The results indicated two different banding patterns AA and AB for this fragment, with the most frequent genotype and allele of AA and A. Calculated Chi-square test for BMPR-1B gene exon-9 was found to be more than that of p value at the 5% level of significance, indicating that the population under study was in Hardy-Weinberg equilibrium for all ewes. The ${\chi}^2$ independence test analyses indicated litter size differences between genotypes was not the same for each breed. The 304-bp nucleotide sequence was subjected to BLAST analysis, and the C864T mutation significantly affected litter size in singletons, twins and multiples. The heterozygosity in exon-9 of BMPR-1B gene could increase litter size for all the studied ewes. Conclusion: Consequently, it appears that the polymorphism BMPR-1B gene exon-9 detected in this study may have potential use in marker assisted selection for litter size in Dorset, Mongolian, and Small Tail Han ewes.

• Asian-Australasian Journal of Animal Sciences
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• 제32권5호
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• pp.691-700
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• 2019

Objective: This study was conducted to assess increasing doses of phytase added to broiler diets formulated with different levels of available phosphorus (avP), calcium (Ca), and sodium (Na), and the respective effects on performance parameters, quantitative carcass characteristics, ash and phosphorus deposition in tibia and weight of organs. Methods: Three different matrices were assumed for phytase with the following nutritional values: matrix A (MT A): 0.165% Ca, 0.150% avP, and 0.035% Na; matrix B (MT B): 0.215% Ca, 0.195% avP, and 0.045% Na; matrix C (MT C): 0.245% Ca, 0.225% avP, and 0.053% Na. There were six different diets: No phytase (formulated to meet the nutritional requirements); phytase 500 FTU/kg+MT A; phytase 1,000 FTU/kg+MT A; phytase 1,500 FTU/kg+MT A; phytase 1,000 FTU/kg+MT B and phytase 1,500 FTU/kg+MT C. Results: There was no significant phytase influence on performance, quantitative carcass characteristics, ash and phosphorus deposition in tibia and weight of the organ throughout the study period, however, it was possible to observe a tendency of improvement in body weight corrected feed conversion for broilers fed the phytase 1,500+MT C diet, where potentially these birds were more efficient on utilize phytic phosphorus and other nutrients bounded to phytate molecule, translating into improvement in performance, and there was also a non significant numerical improvement in body weight corrected feed conversion of broilers fed this diet. Conclusion: Broilers fed with diets formulated with different levels of avP, Ca, and Na and increasing doses of phytase have shown no change on performance, quantitative carcass characteristics, ash and phosphorus deposition in tibia and weight of organs.