• Journal of Microbiology and Biotechnology
• /
• v.15 no.2
• /
• pp.247-253
• /
• 2005

The bacterial cellulose (BC) production by a wild­strain Acetobacter xylinum BPR2001 and that by its acetan­nonproducing mutant, EPI, were compared in a jar fermentor. EPI produced about $28\%$ less BC than the wild-strain. The apparent difference in the cultivation of the two strains was the viscosity increase in the culture broth that was closely associated with acetan production. Increasing the viscosity of the culture broth of EPI by adding agar led to the formation of relatively small and uniform BC pellets, and BC production consequently became two-fold higher than that in the absence of agar and was almost equal to that by BPR2001. Therefore, acetan has an important role in BC production by inducing physical changes in the culture broth of the wild-type strain.

• 한국생물공학회:학술대회논문집
• /
• 2002.04a
• /
• pp.192-195
• /
• 2002

Effect of aeration rate and agitation speed on cell growth and the production of heteropolysaccharide-7 (PS-7) by Beijerinckia indica was investigated. Aeration rate and agitation speed in a 7L bioreactor ranged from 0.5 to 1.5 vvm and from 300 to 500 rpm, respectively. Higher agitation speed with an aeration rate of 0.5 vvm in the bioreactor resulted in maintenance of higher concentration of dissolved oxygen in the medium, which enhanced the production of PS-7. In this study with a 7L bioreactor, maximal production of PS-7 was 11.0 g/L and its conversion rate from 2% (w/v) glucose was 0.55 when the aeration rate and agitation speed were 1.0 vvm and 500 rpm, respectively. Proper aeration rate and agitation speed might enhance the production of PS-7 as well as reduce the time to reach maximal production.

• Journal of Marine Life Science
• /
• v.7 no.2
• /
• pp.121-128
• /
• 2022

L-asparaginase (ASNase) is a therapeutic enzyme used to treat acute lymphoblastic leukemia. Currently, the most widely used ASNases are originated from bacteria. However, owing to the adverse effects of bacterial ASNases, new resources for ASNase production should be explored. Fungal enzymes are considered efficient and compatible resources of natural products for diverse applications. In particular, fungal species belonging to the genus Trichoderma are well-known producers of several commercial enzymes including cellulase, chitinase, and xylanase. However, enzyme production by marine-derived Trichoderma spp. remains to be elucidated. While screening for extracellular ASNase-producing fungi from marine environments, we found four strains showing extracellular ASNase activity. Based on the morphological and phylogenetic analyses using sequences of translation elongation factor 1-alpha (tef1α), the Trichoderma isolates were identified as T. afroharzianum, T. asperellem, T. citrinoviride, and Trichoderma sp. 1. All four strains showed different ASNase activities depending on the carbon sources. T. asperellem MABIK FU00000795 showed the highest ASNase value with lactose as a carbon source. Based on our findings, we propose that marine-derived Trichoderma spp. are potential candidates for novel ASNase production.

• Proceedings of the Korean Society of Crop Science Conference
• /
• 2018.10a
• /
• pp.204-204
• /
• 2018

• 한국생물공학회:학술대회논문집
• /
• 2002.04a
• /
• pp.173-176
• /
• 2002

Effect of carbon source and culture conditions involved in the concentration of dissolved oxygen on cell growth and the production of pullulan by A. pullulans HP2001 were investigated. Among those carbon sources, glucose was found to be the best carbon source for the production of pullulan by A. pullulans HP2001. Maximal production of pullulan by A. pullulans HP2001 was 26.6 g/ f when concentrations of glucose and yeast extract were 8% (w/v) and 0.25% (w/v), respectively. It was found that aeration rate, agitation speed and inner pressure of a bioreactor, which were some of physiological factors involved in the dissolved oxygen in the medium may affect cell growth and the production of pullulan by A. pullulans HP2001.

• Biotechnology and Bioprocess Engineering:BBE
• /
• v.6 no.2
• /
• pp.107-111
• /
• 2001

The addition of a limited concentration of yeast extract to a minimal salt medium (MSM) enhanced cell growth and increased the production of curdlan whereas nitrogen-limitation was found to be essential for the higher production of curdlan by Agrobacterium sp. ATCC 31749. As the amount of the inoculum increased, the cell growth as well as the production of curdlan also increased in the MSM without a nitrogen source. The cell growth and production of curdlan increased as the initial pH of the medium decreased as low as 5.0. The conversion rate and concentration of curdlan from 2% (w/v) glucose in the MSM with concentrated cells under nitrogen deletion was 67% and 13.4 g/L, respectively. The highest conversion rate of curdlan under the conditions optimized in this study was 71% when the glucose concentrations was 1% (w/v).

• Korean Journal of Plant Resources
• /
• v.21 no.6
• /
• pp.444-448
• /
• 2008

An adventitious root formation protocol from Smilax china L. was established for in vitro production of dioscin, a steroidal saponin having various bioactivities such as anticancer, antifungal, antiviral, and antiobesity. Optimal medium for root initiation from leaf explant was MS medium containing $30\;g{\cdot}L^{-1}$ of sucrose supplemented with $1.0\;mg{\cdot}L^{-1}$ kinetin + $2.0\;mg{\cdot}L^{-1}$ NAA. The induction of adventitious roots from in vitro initiated root segments was most favorable to MS liquid medium with $0.1\;mg{\cdot}L^{-1}$ kinetin + $2.0\;mg{\cdot}L^{-1}$ NAA. Among the 20 different adventitious roots originated from different plants, strain No. 10 was selected based on production ability of dioscin, and its stability through the successive suspension culture. The maximum growth stage of adventitious roots was noticed at 5 weeks after subculture while that of dioscin production in the adventitious root was at 7 weeks after subculture in suspension culture system. These results provide that suspension culture of adventitious roots of Smilax china L. have a potential for in vitro mass production of dioscin.

• Journal of Energy Engineering
• /
• v.27 no.3
• /
• pp.68-73
• /
• 2018

The cement industry is one of the rapidly growing industry in Ethiopia. The average per capita cement consumption of the country has increased from 39kg to 62kg. However, this is still way below than the global average per capita consumption of 500kg. The Ethiopian government is planning to expand its cement industry by upgrading the current cement plants and also opening of new cement plants in order to meet the future demand of the country. Currently, the number of cement plants in Ethiopia has reached to 20. By the year 2025, per capita cement consumption is expected to increase to 179kg. Recently, Ethiopia has become one of Africa's largest market for the cement industry. In addition, Ethiopia has become the major exporter of cement in the Sub-Saharan African region. The Ethiopian cement industry is highly dependent on the use of imported energy sources for its production. This situation has a significant amount of impact on the high production costs of the industry. This paper will try to review the history, production, available resources, the technologies and energy use of the Ethiopian cement industry.

• Proceedings of the Korea Society for Simulation Conference
• /
• 2003.11a
• /
• pp.185-192
• /
• 2003

Digital Manufacturing-based production could be very effective in shipbuilding in order to save costs and time, to increase safety for workers, and to prevent bottleneck processes in advance. Digital shipbuilding system, a simulation-based production tool, is being developed to achieve such aspects in Korea. To simulate material flow in a subassembly line at a shipyard, the product, process and resources was modeled for the subassembly process which consisted of several sub-processes such as tack welding, piece alignment, tack welding, and robot welding processes. The analysis and modeling were carried out by using the UML(Unified Modeling Language), an object-oriented modeling method as well as IDEF(Integration DEFinition), a functional modeling tool. Initially, the characteristics of the shop resources were analyzed using the shipyard data, and the layout of the subassembly line was designed with the resources. The production process modeling of the subassembly lines was performed using the discrete event simulation method. Using the constructed resource and process model, the productivity and efficiency of the line were investigated. The number of workers and the variations In the resource performance such as that of a new welding robot were examined to simulate the changes in productivity. The bottleneck process floated according to the performance of the new resources. The proposed model was viewed three-dimensionally in a digital environment so that interferences among objects and space allocations for the resources could be easily investigated

• Proceedings of the Plant Resources Society of Korea Conference
• /
• 2021.04a
• /
• pp.58-58
• /
• 2021

Although hypoxic/ischemic injury is thought to contribute to the incidence of Alzheimer disease (AD), the molecular mechanism that determines the relationship between hypoxia-induced β-amyloid (Aβ) generation and development of AD is not yet known. In this study, we investigated the protective effects of Acorus gramineus Soland. (AGS) on oxygen-glucose deprivation/reoxygenation (OGD/R)-induced A β production in SH-SY5Y human neuroblastoma cells. Pretreatment of these cells with AGS significantly attenuated OGD/R-induced production of reactive oxygen species (ROS) and elevation of levels of malondialdehyde, nitrite (NO), prostaglandin E2 (PGE2), cytokines (TNF-α, IL-1β and IL-6) and glutathione, as well as superoxide dismutase activity. AGS also reduced OGD/R-induced expression of the apoptotic protein caspase-3, the apoptosis regulator Bcl-2, and the autophagy protein becn-1. Finally, AGS reduced OGD/R-induced Aβ production and cleavage of amyloid precursor protein, by inhibiting secretase activity and suppressing the autophagic pathway. Although supporting data from in vivo studies are required, our results indicate that AGS may prevent neuronal cell damage from OGD/R-induced toxicity.