Effect of the aeration rate and agitation speed on heteropolysaccharide-7 production by Beijerinckia indica

  • Jin, Hyeok (Division of Biotechnology, Faculty of Natural Resources and Life Science, Dong-A University) ;
  • Yang, Jae-Gyun (Division of Biotechnology, Faculty of Natural Resources and Life Science, Dong-A University) ;
  • Jeong, Jeong-Han (Division of Biotechnology, Faculty of Natural Resources and Life Science, Dong-A University) ;
  • Jo, Yeong-Su (Division of Biotechnology, Faculty of Natural Resources and Life Science, Dong-A University) ;
  • Lee, Dong-Su (Research Center of Biotechnology & Bioengineering, KBP Co., Ltd.) ;
  • Sin, Myeong-Gyo (Textile and Cleaner Production Center, Korean Institute of Industrial Technology) ;
  • Lee, Jin-U (Division of Biotechnology, Faculty of Natural Resources and Life Science, Dong-A University)
  • Published : 2002.04.12

Abstract

Effect of aeration rate and agitation speed on cell growth and the production of heteropolysaccharide-7 (PS-7) by Beijerinckia indica was investigated. Aeration rate and agitation speed in a 7L bioreactor ranged from 0.5 to 1.5 vvm and from 300 to 500 rpm, respectively. Higher agitation speed with an aeration rate of 0.5 vvm in the bioreactor resulted in maintenance of higher concentration of dissolved oxygen in the medium, which enhanced the production of PS-7. In this study with a 7L bioreactor, maximal production of PS-7 was 11.0 g/L and its conversion rate from 2% (w/v) glucose was 0.55 when the aeration rate and agitation speed were 1.0 vvm and 500 rpm, respectively. Proper aeration rate and agitation speed might enhance the production of PS-7 as well as reduce the time to reach maximal production.

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