Opitimization of culture conditions involved in dissolved oxygen for production of pullulan by Aureosidium pullulan

  • Seo, Hyeong-Pil (Division of Biotechnology, Faculty of Natural Resources & Life Science, Dong-A University) ;
  • Son, Chang-U (Division of Biotechnology, Faculty of Natural Resources & Life Science, Dong-A University) ;
  • Lee, Yu-Jeong (Division of Biotechnology, Faculty of Natural Resources & Life Science, Dong-A University) ;
  • Kim, Seong-Gu (Division of Food & Biotechnology, Pukyung National University) ;
  • Kaplan, David L. (Biotechnology Center, Tufts University) ;
  • Lee, Jin-U (Division of Biotechnology, Faculty of Natural Resources & Life Science, Dong-A University)
  • Published : 2002.04.12

Abstract

Effect of carbon source and culture conditions involved in the concentration of dissolved oxygen on cell growth and the production of pullulan by A. pullulans HP2001 were investigated. Among those carbon sources, glucose was found to be the best carbon source for the production of pullulan by A. pullulans HP2001. Maximal production of pullulan by A. pullulans HP2001 was 26.6 g/ f when concentrations of glucose and yeast extract were 8% (w/v) and 0.25% (w/v), respectively. It was found that aeration rate, agitation speed and inner pressure of a bioreactor, which were some of physiological factors involved in the dissolved oxygen in the medium may affect cell growth and the production of pullulan by A. pullulans HP2001.

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