Biotechnology and Bioprocess Engineering:BBE

The Korean Society for Biotechnology and Bioengineering (한국생물공학회)
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Improved Production of Curdlan with Concentrated Cells of Agrobacterium sp.

  • Jung, Dae-Young (Division of Biotechnology, Faculty of Natural Resources and Life Science) ;
  • Cho, Young-Su (Division of Biotechnology, Faculty of Natural Resources and Life Science) ;
  • Chung, Chung-Han (Division of Biotechnology, Faculty of Natural Resources and Life Science) ;
  • Jung, Dai-Il (Department of Chemistry, College of Natural Science) ;
  • Kim, Kwang (Department of Chemical Engineering, College of Engineering, Dong-A University) ;
  • Lee, Jin-Woo (Division of Biotechnology, Faculty of Natural Resources and Life Science)
  • Published : 2001.03.01
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Abstract

The addition of a limited concentration of yeast extract to a minimal salt medium (MSM) enhanced cell growth and increased the production of curdlan whereas nitrogen-limitation was found to be essential for the higher production of curdlan by Agrobacterium sp. ATCC 31749. As the amount of the inoculum increased, the cell growth as well as the production of curdlan also increased in the MSM without a nitrogen source. The cell growth and production of curdlan increased as the initial pH of the medium decreased as low as 5.0. The conversion rate and concentration of curdlan from 2% (w/v) glucose in the MSM with concentrated cells under nitrogen deletion was 67% and 13.4 g/L, respectively. The highest conversion rate of curdlan under the conditions optimized in this study was 71% when the glucose concentrations was 1% (w/v).

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