• 한국미생물·생명공학회지
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• 제50권4호
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• pp.508-511
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• 2022

식물성 육류에서 미각적인 자극을 얻기 위해 사용하는 heme 함유 단백질의 지속적인 생산을 위해 강낭콩 유래 leghemoglobin a (PhLba) 유전자를 pPICZαA에 클로닝하고 Pichia pastoris에서 발현하였다. 재조합 PhLba 단백질은 가용화 형태로 배양 배지속으로 분비되었다. 정제된 PhLba의 분자량은 SDS-PAGE 상에서 16.5 kDa으로 나타났다. 재조합 PhLba holoprotein의 수율은 배양 배지에 hemin을 첨가함으로써 향상되었다. 이것은 PhLba의 apo 형태가 보조인자와 함께 효과적으로 포화된다는 것을 나타낸다.

• 한국작물학회:학술대회논문집
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• 한국작물학회 2022년도 추계학술대회
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• pp.299-299
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• 2022

Whole wheat is rich in dietary fiber and contains various biological activity substances such as arabinoxylan, phytic acid and phenolic compounds. However, excessive fiber contents of whole wheat has a negative effect on dough formation, making it difficult to process. In this study, we tried to improve the usability of whole wheat by suggesting an appropriate degree of purification of whole wheat from 'Saekeumkang', a domestic wheat cultivar containing protein and gluten suitable for noodle production. The contents of arabinoxylan, phytic acid, and vitamin E were measured in the polishing rate range of 5-20% of whole wheat flour. As the milling ratio increased, the flour properties improved. The arabinoxylan and phytic acid content of whole wheat were 67.95 mg/g and 0.87 mg/g, but when milled at 20%, arabinoxylan and phytic acid were 60% and 80% of whole wheat, respectively. And as the milling ratio increased, the vitamin E content tended to decrease (whole wheat: 4.063 mg/100 g, 20% milled: 2.96 mg/100 g), However, the vitamin E composition ratio did not change. On the other hand, α-tocopherol showed the greatest than other vitamin E isomers. Therefore, further studies needed to optimize milling rate to improve the final product while maintaining the approximate nutritional and functional value of the whole wheat.

• Advances in nano research
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• 제15권5호
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• pp.441-449
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• 2023

Heavy metals, widely present in the environment, have become significant pollutants due to their excessive use in industries and technology. Their non-degradable nature poses a persistent environmental problem, leading to potential acute or chronic poisoning from prolonged exposure. Recent research has focused on separating heavy metals, particularly from industrial and mining sources. Industries such as metal plating, mining operations, tanning, wood and chipboard production, industrial paint and textile manufacturing, as well as oil refining, are major contributors of heavy metals in water sources. Therefore, removing heavy metals from water is crucial, especially for safe water supply in swimming and water sports. Iron oxide nanoparticles have proven to be highly effective adsorbents for water contaminants, and efforts have been made to enhance their efficiency and absorption capabilities through surface modifications. Nanoparticles synthesized using plant extracts can effectively bind with heavy metal ions by modifying the nanoparticle surface with plant components, thereby increasing the efficiency of heavy metal removal. This study focuses on removing lead from industrial wastewater using environmentally friendly, cost-effective iron nanoparticles synthesized with Genovese basil extract. The synthesis of nanoparticles is confirmed through analysis using Transmission Electron Microscope (TEM) and X-ray diffraction, validating their spherical shape and nanometer-scale dimensions. The method used in this study has a low detection limit of 0.031 ppm for measuring lead concentration, making it suitable for ensuring water safety in swimming and water sports.

• 한국식품영양학회지
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• 제22권2호
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• pp.261-271
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• 2009

Deep sea water exists at depths of over 200m under the sea. As no sunlight reaches it, photosynthesis does not take place within it, and it contains no organic matter. In addition, its temperature is maintained at a stable low level throughout the year, so it does not get mixed with the sea water on the surface. It contains a large amount of nutritious salts, whose cleanness is maintained. It is a marine resource that has matured for a long period of time. Research into deep sea water, which started in the 1970s, has been made around the whole world, including the USA and Japan. In Korea, research has been active in this area since 2000. As there has been a good amount of research into industrial applications for deep sea water, since 1993, patents for the relevant technologies have been applied. This paper intends to provide a resource to researchers of deep sea water, by summarizing of all domestic deep sea water-related patents applied with Korean Intellectual Property Office from 1993 to 2008. This research was conducted using a computer and KIPRIS Database owned by the Korea Institute of Patent Information. 'Deep sea water' was used as the search keyword. A total of 222 Korean patents relating to deep sea water have been registered on the basis of IPC. Of these, 126 patents relate to the manufacturing and the treatment of foods, foodstuffs, or non-alcoholic beverages(A23L), while 50 patents relate to the production for medical, dental, or cosmetic purposes(A61K). 38 patents relate to water purification, treatment of wastewater, sewage and sludge (C02F), while 8 patents relate to fishery and farming(A01K). In summary, it was found that studies for the practical use of deep sea water have been conducted in relation to the manufacturing and the treatment of foods, foodstuffs, beverages, and cosmetics.

• 생약학회지
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• 제37권4호
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• pp.221-228
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• 2006

A lectin was purified from Serrognathus platymelus castanicolor larvae and named as SPL. The purification was carried out by ion-exchange chromatography on DEAE Sephadex A-50 and gel filtration chromatography on Sephadex G-200. The purity of the protein was verified by polyacrylamide gel electrophoresis and the purified lectin agglutinated erythrocytes of rabbit and human A, B, O, AB. SPL was tested it's ability to enhance the expressions of cytokines, $IL-1\alpha$, IL-2, IL-6, $TNF\alpha$ and $IFN\gamma$ by human peripheral blood mononuclear cells (PBMC) obtained from healthy donors. mRNA analyses were performed by RT-PCR at the moment of 1, 4, 8, 24, 48, 72 and 96 h after stimulation of PBMC with purified SPL. The patterns of IL-2 band were slightly expressed from 24 h and the strongest band was appeared at 96 h. The expressions of $IL-1\alpha$ and IL-6 mRNA were strong from 1 to 8 h and those of $TNF\alpha$ were from 48 to 96 h. The mRNA encoding $IFN\gamma$ were not detected. The addition of SPL for macrophage cultures induced production of nitric oxide (NO) by cells in a dose-dependent manner. NO release was partially inhibited by $TNF\alpha$ antibodies. These results suggest that SPL has the ability to enhance cytokine expressions in PBMC and to induce the NO release by TNFa in macrophage cultures from PBMC cultures.

• Journal of Microbiology and Biotechnology
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• 제14권6호
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• pp.1142-1149
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• 2004

Streptococcus mutans has the capacity of inducing dental caries. Thus, to develop a novel way of preventing dental caries, a cell wall hydrolase-producing strain was isolated and its characteristics were investigated. Among 200 alkalophilic strains isolated from soil, 8 strains exhibited lytic activities against Streptococcus mutans. However, strain YU5215 with the highest cell wall hydrolase activity was selected for further study. Strain YU5215 was identified as a novel strain of Bacillus based on analyzing its 16S rDNA sequence and Bergey's Manual of Systematic Bacteriology, and thus designated as Bacillus mutanolyticus YU5215. The optimal conditions for the production of the cell wall hydrolase from Bacillus mutanolyticus YU5215 consisted of glucose ($0.8\%$), yeast extract ($1.2\%$), polypeptone ($0.5\%$), $K_{2}HPO_{4}\;(0.1\%$), $MgSO_{4}{\cdot}7H_{2}O$ ($0.02\%$), and $Na_{2}CO_{3}\;(1.0\%$) at pH 10.0. Bacillus mutanolyticus YU5215 was cultured at 30^{circ}C for 72 h to produce the cell wall hydrolase, which was then purified by acetone precipitation and CM-agarose column chromatography. The molecular weight of the lytic enzyme was determined as 22,700 Da by SDS-PAGE. When the cell wall peptidoglycan of Streptococcus mutans was digested with the lytic enzyme, no increase in the reducing sugars was observed, while the free amino acids increased, indicating that the lytic enzyme had an endopeptidase-like property. The amino terminus of the cell wall peptidoglycan digested by the lytic enzyme was determined as a glutamic acid, while the lytic site of the lytic enzyme in the Streptococcus mutans peptidoglycan was identified as the peptide linkage of L-Ala and D-Glu.

• 대한수의학회지
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• 제26권1호
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• pp.97-102
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• 1986

The prevalence of diarrhea caused by enterotoxigenic Escherichia coli was surveyed on 445 calves in 6 farms which were located in the central part of Korea. The incidence of enterotoxigenic Escherichia coli in calves with diarrhea was investigated by detecting the K99 and F41 antigens from the isolated strains of Escherichia coli The incidence of colibacillosis in calves was 23.3%. Of 238 strains of Escherichia coli isolated from calves with diarrhea, 73 strains(30.6%) were proved possessing the K99 antigen by mannose-resistant hemagglutination(MRHA) using horse red blood cells and 79(33.1%) possessing F41 antigens by MRHA using guinea-pig red blood cells. The minca medium, nutrient broth, tryptic soy broth and brain heart infusion were tested for yield of K99 and F41 pili. The production of pili was greatest in minea medium. The best detachment method of the K99 and F41 pili from the cells was heat treatment for 20 minutes at $60^{\circ}C$ and concentration by precipitation with ammonium sulfate. The purified antigens of K99 and F41 were polypeptides with molecular weights of 18,500 and 29,500, respectively by sodium dodecyl sulfate-polyacrylamide gel electrophoresis.

• 한국식품과학회지
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• 제26권4호
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• pp.375-381
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• 1994

토양을 대상으로 하여 CGTase를 생산하는 균주를 분리, 선별하여 Bacillus stearothermophilus KY-126을 얻었다. CGTase의 정제는 ammonium sulfate precipitation, ion exchange chromatography, gel filtration의 과정을 통해 분리 정제하여 단일 효소를 얻었으며, 분자량은 약 67,000이었다. 효소 반응의 최적 온도는 $65^{\circ}C$였으며, $55^{\circ}C$에서 30분간 열처리에도 비교적 열에 안정하였다. 최저 활성 pH는 5.5였고 pH5.5에서 10.5까지 비교적 안정하였다. $HgCl_{2}$에 의해 저해를 받았으며, 그 외의 금속 이온에는 저해를 받지 않았다. Soluble starch로부터 CD의 전환율은 43%이었으며, ${\alpha}-:,\;{\beta}-:,\;{\gamma}-$, CD의 생성 비율은 2.9 : 2.1 : 1이었다.

• 한국미생물·생명공학회지
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• 제43권4호
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• pp.330-335
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• 2015

Bacillus agaradhaerens 균주의 효소를 정제하여 그 특성을 확인하였다. 균주가 생산하는 xylanase를 70% ammonium sulfate로 침전하고 CM-sepharose ion exchange chromatography와 ultrafiltration(PM-10)을 행하여 16.7%의 수율과 6.7배의 정제도를 지니는 효소를 얻을 수 있었으며 SDS-PAGE를 통하여 23 kDa의 분자량을 갖는 효소임을 확인하였다. 효소의 최적 pH는 6.0으로서 sodium phosphate buffer에서 24시간까지 안정하였으며, 최적반응온도는 $60^{\circ}C$, EDTA의 첨가 시 효소활성이 증가되었고 $40^{\circ}C$에서 24시간까지 안정하였다. 효소는 xylan만을 기질로 이용할 수 있었으며 Birchwood xylan에 대하여 최대 활성을 나타내었고 $V_{max}$는 $49,724{\mu}M/min$, $K_m$은 6.08 mg/ml로 확인되었다.

• 한국융합학회논문지
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• 제8권12호
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• pp.1-7
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• 2017

본 연구는 환경정화에 주로 사용되는 미세기포를 2년 근 인삼 지속적으로 처리하여 인삼이 성장하면서 변화되는 형태와 ginsenoside 변화를 조사하는 융합적 연구이다. 인삼 재배시 미세기포수를 적용하여 4개월 동안(120일) 재배한 후 인삼의 잎과 뿌리의 부위별 ginsenoside 함량과 조성을 분석하였다. 잎에 일반수를 처리한 결과 protopanaxatriol(PPT) 계열 Re 함량만 월등히 높게 나타났지만 미세기포수를 처리한 결과 protopanaxadiol(PPD) 계열 Rb1, RC, Rb2, Rd 성분도 같이 증가시키는 것을 확인하였다. 특히 Re, Rb1이 다량 증가함으로써 전체적인 total ginsenoside가 증가하는 요인이 되었다. 인삼의 부위별 PD/PT 비율은 미세기포수를 처리한 잎에서는 0.811으로 나타나고 뿌리는 1.28로 나타났다. 이것은 미세기포수 처리가 뿌리에서 ginsenoside의 합성을 유도하여 PD/PT 비율이 1과 가까운 결가를 가져와 유용성분의 증가 및 고른 분포 이루어졌다고 판단된다. 따라서 미세버블수를 사용한 고품질 인삼을 생산하는 재배 방법을 제시하고 인삼의 뿌리와 더불어 잎도 기능성 식품 소재로 활용할 수 있는 가능성을 제시하였다.