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http://dx.doi.org/10.4014/mbl.1511.11002

Characterization of Xylanase from Bacillus agaradhaerens DK-2386 Isolated from Korean Soil  

Choi, Ji-Hwi (Department of Food Engineering, Dankook University)
Park, Young-Seo (Department of Food Science and Biotechnology, Gachon University)
Lee, Hyungjae (Department of Food Engineering, Dankook University)
Bai, Dong-Hoon (Department of Food Engineering, Dankook University)
Publication Information
Microbiology and Biotechnology Letters / v.43, no.4, 2015 , pp. 330-335 More about this Journal
Abstract
The optimum conditions for the production of xylanase from Bacillus agaradhaerens DK-2386 have been previously investigated. In this study xylanase was purified by ammonium sulfate precipitation and CM-sepharose ion exchange chromatography. The molecular mass of the xylanase as determined by SDS-PAGE was 23 kDa in a form of monomeric enzyme. The optimum pH and temperature for xylanase activity was 6.0 and $60^{\circ}C$, respectively. Xylanase activity was increased by the addition of EDTA and then stabilized at $40^{\circ}C$ for 24 h. The maximum xylanase activity was obtained when Birchwood xylan was used as a substrate and the $V_{max}$ and $K_m$ were $49,724{\mu}mol/min$ and 6.08 mg/ml, respectively.
Keywords
Bacillus; xylanase; purification; activity;
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