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Effect of Lectin Isolated from Serrognathus platymelus castanicolor Larvae on the Various Cytokine Expressions  

Jo, Su-Hyun (College of Sciences, Yeungnam University)
Kim, Se-Jin (College of Sciences, Yeungnam University)
Chung, See-Ryun (College of Pharmacy, Yeungnam University)
Jeune, Kyung-Hee (College of Sciences, Yeungnam University)
Publication Information
Korean Journal of Pharmacognosy / v.37, no.4, 2006 , pp. 221-228 More about this Journal
Abstract
A lectin was purified from Serrognathus platymelus castanicolor larvae and named as SPL. The purification was carried out by ion-exchange chromatography on DEAE Sephadex A-50 and gel filtration chromatography on Sephadex G-200. The purity of the protein was verified by polyacrylamide gel electrophoresis and the purified lectin agglutinated erythrocytes of rabbit and human A, B, O, AB. SPL was tested it's ability to enhance the expressions of cytokines, $IL-1\alpha$, IL-2, IL-6, $TNF\alpha$ and $IFN\gamma$ by human peripheral blood mononuclear cells (PBMC) obtained from healthy donors. mRNA analyses were performed by RT-PCR at the moment of 1, 4, 8, 24, 48, 72 and 96 h after stimulation of PBMC with purified SPL. The patterns of IL-2 band were slightly expressed from 24 h and the strongest band was appeared at 96 h. The expressions of $IL-1\alpha$ and IL-6 mRNA were strong from 1 to 8 h and those of $TNF\alpha$ were from 48 to 96 h. The mRNA encoding $IFN\gamma$ were not detected. The addition of SPL for macrophage cultures induced production of nitric oxide (NO) by cells in a dose-dependent manner. NO release was partially inhibited by $TNF\alpha$ antibodies. These results suggest that SPL has the ability to enhance cytokine expressions in PBMC and to induce the NO release by TNFa in macrophage cultures from PBMC cultures.
Keywords
lectin; Serrognathus platymelus castanicolor; cytokine; immunomodulator; nitric oxide;
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1 Elgavish, S. and Shaanan, B. (1997) Lectin-carbohydrate interactions: different folds, common recognition principles. Trends in Biochem. Sci. 22: 462-467   DOI   ScienceOn
2 Chung, S. R. and Jeune, K. H. (1986) Lectins. Review of Biochem. Biochem. Soc. Kor. 1: 371-382
3 Jeune, K. H., Jung, M. Y., Choi, S. J., Lee, J. W., Park, W. H., Cho, S. H., Lee, S. H. and Chung, S. R. (2001) Immunomodulating effect of the lectin from Allomyrina dichotoma. Kor. J. Pharmacogn. 32: 31-38
4 Bredt, D. S. and Snyder, S. H (1990) Isolation of ntric oxide synthetase, a calmodulin-requiring enzyme. Proc. Natl. Acad. Sci. U.S.A. 87: 682
5 Knoxles, R. G, Salter, M., Brooks, S. L. and Moncada, S. (1990) Anti-inflammatory glucocorticoids induction by endotoxin of nitric oxide synthase in the lung, liver and aorta of the rat. Biochem. Biophys. Res. Commun. 172: 1042   DOI   ScienceOn
6 Kim, H. S., Sung, Y. O., Lee, Y S. and Kim, S. K. (1995) Candida albicans-induced TNF$\alpha$ production from human peripheral blood lymphocytes. Korea J. Microbiol. 30: 1-12
7 Lowenstein, C. J., Glatt, C. S., Bredt, D. S. and Snyder, S. H. (1992) Cloned and expressed macrophage nitric oxide synthase contrasts with the brain enzyme. Proc. Natl. Acad. Sci. U.S.A. 89: 6711
8 Lowry, O. H., Rosebrough, N. J., Farr, A. L. and Randall, J. (1951) Protein measurement with folin phenol reagent. J. Biol. Chem. 193: 265
9 Rees, D. D., Cellek, S., Palmer, R. M. J. and Moncada, S. (1990) Dexamethasone prevents the induction by endotoxin of a nitric oxide synthase and the associated effect on vascular tone: an insight into endotoxic shock. Biochem. Biophys. Res. Commun. 173: 541   DOI   ScienceOn
10 Lee, I. K., Kim, H. S., Jeune, K. H, Kim, S. K. and Chung, S. R. (1995) Effects of Lentinus edodes lectin on cytokine gene expression from human peripheral blood mononuclear cells. J. Korean Soc. Microbiol. 30: 473-481
11 Green, L. C, Wagner, D. A., Glogowski, J., Skipper, R L., Wishnok, J. S. and Tannenbaum, S. R. (1982) Analysis of nitrate, nitrite, and $[^{15}N]$ nitrate in biological fluids. Anal. Biochem. 126: 131   DOI   ScienceOn
12 Jeune, K. H., An, M. G, Jung, S. M, Choi, K. M., Lee, S. H. and Chung, S. R. (1999) Effect of mung bean lectin (MBL) on cytokine gene expression from human peripheral blood mononuclear cells. Kor. J. Pharmacogn. 30: 355-362   과학기술학회마을
13 Jeune, K. H, Choi, S. J. and Chung, S. R. (1999) Effect of Asterina pectinifera Lectin on cytokine production. Yakhak Hoeji 43: 474-480   과학기술학회마을
14 Goldstein, I. J., Hughes, R. C, Monsigny, M., Osawa, T. and Sharon, N. (1980) What should be called a lectin? Nature 285: 66
15 Ma, J. J. and Byun, K. H. (1994) Immune Response Regulation and Immunotheraphy. Genetic Engineering Research Institute KIST. 5-15
16 Davis, B. J. (1964) Disc electrophoresis II. Ann. N. Y. Acad. Sci. 121: 404   DOI
17 Jeune, K. H., Kim, S. J. and Chung, S. R. (2005) Cytokine expressions with lectins from Allomyrina dichotoma and Bombyx mori. Kor. J. Pharmacogn. 36: 129-135   과학기술학회마을
18 Kim, J. Y., Lee, H. J., Kim, J. S., Ahn, H. N. and Ryn, J. H. (2005) A ginseng saponin induces production of nitric oxide in macrophges, Yakhak Hoeji 49: 80-85   과학기술학회마을