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http://dx.doi.org/10.48022/mbl.2211.11001

Production, Purification, and Characterization of Phaseolus vulgaris Leghemoglobin a in Pichia pastoris  

Jun-Young, Kim (Biomedical Engineering and Biotechnology Major, Division of Applied Bioengineering,Dong-Eui University)
Da-Hee, Han (Biomedical Engineering and Biotechnology Major, Division of Applied Bioengineering,Dong-Eui University)
Geun-o, Park (Biomedical Engineering and Biotechnology Major, Division of Applied Bioengineering,Dong-Eui University)
Soo-Wan, Nam (Biomedical Engineering and Biotechnology Major, Division of Applied Bioengineering,Dong-Eui University)
Yeon-Hee, Kim (Biomedical Engineering and Biotechnology Major, Division of Applied Bioengineering,Dong-Eui University)
Han-Woo, Kim (Division of Life Sciences, Korea Polar Research Institute (KOPRI))
Sung-Jong, Jeon (Biomedical Engineering and Biotechnology Major, Division of Applied Bioengineering,Dong-Eui University)
Publication Information
Microbiology and Biotechnology Letters / v.50, no.4, 2022 , pp. 508-511 More about this Journal
Abstract
In this study, Phaseolus vulgaris (kidney bean) leghemoglobin a (PhLba) gene was cloned into pPICZαA and expressed in Pichia pastoris to sustainably produce a heme-carrying protein for organoleptic use in plant-based meat. The recombinant PhLba protein was secreted into the culture medium in a solubilized form, and the molecular weight of the purified PhLba was estimated to be 16.5 kDa using SDS-PAGE. In addtion, the yield of recombinant PhLba holoprotein was enhanced by supplementation of the cultivation medium with hemin. This result indicates that the apo-forms of PhLba can be effectively saturated with cofactor.
Keywords
Leghemoglobin; heme; cofactor; Phaseolus vulgaris; Pichia pastoris;
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Times Cited By KSCI : 1  (Citation Analysis)
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