• Journal of Microbiology and Biotechnology
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• v.30 no.2
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• pp.196-205
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• 2020

In this study, the acidic lipase from Aspergillus niger (ANL) was homologously expressed in A. niger. The expression of ANL was significantly improved by the expression of the native ANL with the introns, the addition of the Kozak sequence and the optimization of the signal sequences. When the cDNA sequence of ANL fused with the glaA signal was expressed under the gpdA promoter in A. niger, no lipase activity could be detected. We then tried to improve the expression by using the full-length ANL gene containing three introns, and the lipase activity in the supernatant reached 75.80 U/ml, probably as a result of a more stable mRNA structure. The expression was further improved to 100.60 U/ml by introducing a Kozak sequence around the start codon due to a higher translation efficiency. Finally, the effects of three signal sequences including the cbhI signal, the ANL signal and the glaA signal on the lipase expression were evaluated. The transformant with the cbhI signal showed the highest lipase activity (314.67 U/ml), which was 1.90-fold and 3.13-fold higher than those with the ANL signal and the glaA signal, respectively. The acidic lipase was characterized and its highest activity was detected at pH 3.0 and a temperature of 45℃. These results provided promising strategies for the production of the acidic lipase from A. niger.

• Asian-Australasian Journal of Animal Sciences
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• v.21 no.2
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• pp.270-276
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• 2008

The purpose of the present study was to investigate the effects of exogenous non-starch polysaccharides (NSP) enzymes on performance, intestinal content viscosity and digestive enzyme activities of growing pigs fed a rough rice-based diet. A total of 60 crossbred barrows with an initial body weight of 35.16 kg (SD = 0.82) were blocked by body weight and randomly assigned to two treatments with three replications. Each group was fed the diet based on rice with or without exogenous NSP enzymes (2 g/kg of diet). During the 70 days of the feeding trial, all pigs were given free access to feed and water. At the end of the feeding trial, six pigs from each treatment were randomly selected and slaughtered to collect intestinal digesta, intestinal mucosa, and pancreas. The addition of NSP enzymes improved average daily gain (p<0.05) and feed:gain (p<0.05), and decreased viscosity of digesta in the jejunum (p<0.001) and ileum (p<0.01) of pigs. The supplementation of NSP enzymes increased activities of protease (p<0.01), trypsin (p<0.01) and ${\alpha}$-amylase (p<0.05) in duodenal contents. However, digestive enzymes in the pancreas, jejunal and ileal mucosa were unaffected by the supplemental NSP enzymes (p>0.10). The results indicate that the addition of NSP enzymes to rough rice-based diets improved performance of pigs, reduced viscosity and increased digestive activity in the small intestine.

• CELLMED
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• v.5 no.4
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• pp.23.1-23.6
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• 2015

Complementary and alternative medicine (CAM) practice is still popular among the Malaysian population nowadays although western or allopathic medicine is the first line of treatment. Dissatisfaction with health services and therapeutic effects of western medicine or preference for holistic, integrative approach in treatment are common reasons favouring the increasing popularity of CAM practices. The efforts toward integration of CAM and western medicine in Malaysia were rather slow and in a piece-meal fashion. Strategic efforts in strengthening government and self-regulation among practitioners, formalizing education, promoting research, and cultivating national and international networks are necessary to achieve an integrative system. Regulations to restrict the practice and sale of CAM products to licensed practitioners, strict and mandatory registration of the practitioners, inclusion of CAM in essential medicines list, and pricing regulations must be comprehensively discussed. Development of curriculum, offers of scholarship and incentives, promotion of courses and seminars for professionals is necessary to increase the numbers of CAM experts. Malaysia should follow the efforts of other countries on the production and documentation of local CAM data, allocation of funding, and establishment of research centres to assess the efficacy of potentially useful local products. Local and international collaboration in research and continuous education is important for exchange of knowledge and skills. In conclusion more coordinated efforts in regulation of CAM practice and products, formalizing CAM training and education would significantly move the process forward and allow the public to enjoy more health benefits from CAM practice in Malaysia.

• The Journal of the Korea Contents Association
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• v.13 no.8
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• pp.180-190
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• 2013

This study is designed to introduce 'news literacy' as a theoretical framework to evaluate news education in digital age. Specifically, this study conceptually defined news literacy as news literacy self-efficacy and empirically tested how this variable is associated with other related determinants including users' news media consumption pattern, news knowledge structure on news content production, and involvement with news. The findings showed that users' 'searching/understanding' news literacy self-efficacy was positively affected by their knowledge structure and involvement with news, whereas users' 'sharing/expressing' news literacy self-efficacy was positively affected by their knowledge structure. The findings also demonstrated that users' 'searching/understanding' news literacy self-efficacy positively influenced their consumption of new types of news media, whereas users' 'sharing/expressing' news literacy self-efficacy positively influenced their consumption of traditional news media. As a result, this study practically suggests how news literacy is associated with the important factors affecting news consumption and news education.

• Journal of the Korean Institute of Educational Facilities
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• v.2 no.1
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• pp.7-14
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• 1995

The importance of church school education is becoming more emphasized. To maximize the effectiveness and efficiency of church school education, frequent utilization of instructional media is more desirable. Systematic approach to media utilization will be developed by the extabilishment of church media center. The purpose of this study was to review the concept and components of church media center and to survey the current status and hope for the facility of church media center. For the survey the 400 questionnaries were distributed to pastors or missionaries who are responssible for education, or personnels in church media center in Korea. The churches that they belong to have above approximately 500 church members. Among the distributed questionnaires, the 202 were collected. The results of the survey are as follows. The printed media and the AV media were managed separately in most churches. Many church media center was shared the space with other programs. The space was allocated mainly for storage. Most respondants hoped that they have a variety of areas in their churches. Many church media center have no separation between the area for the adults and that for children. However, most respondants hoped the separation of that areas. Followings are suggestions for the plan of church media center facilities. The space only for the church media center should be allocated. Church media center should be located in the first floor of the building as soon as possible. (If there is elevators in the building, it may be located above first floor). If enough space for the church media center is allocated, the areas for the adults and children should be separated by partitions or separate room. The space should consist of various areas like circulation area, reading area, working area for the personnel, production area, and storing area.

• Journal of the Korea Fashion and Costume Design Association
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• v.10 no.3
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• pp.111-126
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• 2008

This article aims to discover the current status and appropriate way to research Gyubang craft, expecting its development and preservation, and elevating it to Korea's unique artistic textile culture. It also searches improved methods for Gyubang craft as the following: First, in order to avoid confusions about Gyubang craft, appropriate concepts and definitions of Gyubang craft must be settled through a discussion among experts. Second, by activating economic activities at lower ratio, among purposes of Gyubang craft, achievements must be elevated. Third, in order to settle down the difficulties of Gyubang craft, a variety of methods such as effective sewing machines and manufacturing techniques as well as providing materials at a reasonable price, considering practical ways through diversifying products, elevating the economy of crafts, exploring sales routes, improving educational contents, and expanding educational places must be facilitated. Fourth, for nurturing talented and excellent human resources in the area of Gyubang craft, the followings should be considered: diversifying contents of education, developing textbooks, improving instructors' quality, providing reasonable cost of education, educating on the utilization and application of Gyubang craft, introducing efficient teaching methods through textbooks and multimedia, awarding 'certificates' to trainees, and permitting certificate holders' to instruct Gyubang craft. Finally, it is convinced that the achievement of production, pride of preserving tradition, pleasure of procedural work, economic activities as experts, and sales of products will be reinforced, and the population for Gyubang craft is also expected to increase. By elevating Gyubang craft into Korea's traditional textile art, Korea's global competitiveness in the area of textile should be strengthened.

• Journal of the Korean Home Economics Association
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• v.44 no.12
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• pp.9-18
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• 2006

A case study was conducted on the development of patient clothes designs at the Korea University Medical Center, in order to provide an example of patient clothes designs which meet the hospital identity and the demands of patients and nurses. In this study, we focused on general patient pajamas and one-piece gowns worn in the obstetrics and gynecology department. A scientific and systematic design approach consisting of the following four steps was conducted in the designing process: needs assessment, design direction establishment, design presentation, and final decision. For the first step, focused group interviews and a survey were conducted to identify the needs of the patients and nurses. According to the results of the first step, 3 fabric patterns with light, modern, rhythmic, modest and ordinary images were designed utilizing the university and hospital symbols and logotypes. Fixed forms with realistic and geometric characteristics and colors, including white, yellow, blue, grey, and pink were selected to deliver the preferred image. Each fabric pattern was made in male and female versions of the color sets. The following styles of patient clothes were made with the 3 fabric samples: general patient pajamas with a U-neckline, 9/10-length sleeves and ankle-length pants, a full-length sleeved gown with openings for breast-feeding, and a gown with a deep back-neckline and button fastening. Different sizing systems for males and females were recommended for the production.

• The Journal of Korean Association of Computer Education
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• v.8 no.4
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• pp.57-69
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• 2005

In this study we try to design and develop a mixed model of project-based learning with internet. In order to support the developed model, we separately developed a web-based tool, called Project Learning BBS. The classroom teaching for advertisement-photo production with the model has been carried out during May-July in 2002 and March-July in 2003 respectively with 40 students each. The overall activities of group collaborative work done during the teaching periods have been formatively evaluated with classroom observation, interviews and students' portfolios that were related to the processes of photo planning, video shooting and editing. It has also investigated how the students involved the web-based group discussion activities. In short, data analysis indicated that the participants accepted the proposed project-based learning model for their learning events in a positive way in order to increase the utilization of CMC.

• Journal of the Korean Society of Radiology
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• v.12 no.1
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• pp.71-78
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• 2018

In this study, human mimic phantoms outputted by three-dimensional (3D) printing technology are reported. Polylactic acid and a personal 3D printer - fused deposition modeling (FDM) - are used as the main material and the printing device. The output of human mimic phantoms performed in the following order: modeling, slicing and G-code conversion, output variable setting, 3D output, and post-processing. The students' learning satisfaction (anatomical awareness, study interest) was measured on 5-point Likert scale. After that, Twenty of those phantoms were outputted. The total output took 11,691 minutes (194 hours 85 minutes) and the average output took 584.55 minutes (9 hours 7 minutes). The filament used for the experiment was 2,390.2 g, and the average use of the filament was 119.51 g. The learning satisfaction of anatomical awareness was 4.6 points on the average and the interest of the class was on average 4.5 points. It is expecting that 3D printing technology can enhance the learning effect of imaging anatomy education.

• Korean journal of food and cookery science
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• v.25 no.3
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• pp.357-364
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• 2009

The objectives of this study was to explore the knowledge, attitudes, and perceptions of elementary school students towards trans fatty acids. In addition, the students' preferences and consumption frequencies of processed food items containing trans fats, as well as their food behaviors relating to snack consumption, were investigated. The study population consisted of students from seven provinces, including Seoul, Busan, Daegu, Kwangju, Gyungnam, Chungnam, and Kangwon. Two schools were selected in each province and one class was randomly chosen in each school. All students from the selected classes were surveyed onsite (N=951). The data were analyzed with chi-square tests and t-tests using SPSS/Windows (ver.15.0) program. The results showed that the students' knowledge levels toward trans fatty acid were very low, particularly on the production process of trans fats. The students perceived that trans fatty acids can cause health problems and should be consumed in low amounts. They also felt there is a need for a good nutrition education on trans fats. Furthermore, the students responded that their most preferred foods were in the order of breads, biscuits, and snacks. The most frequently consumed snack item among 2nd graders was biscuits and among 5th graders it was breads. It was also found that 90% of the students had snacks once a day, and of them, 58% prepared their own snacks. Based on this, we propose that students who allowed to choose their own snacks should be carefully educated on trans fatty acids to prevent excessive consumption of high trans fat foods.