• The Korean Journal of Nuclear Medicine
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• v.38 no.1
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• pp.62-73
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• 2004

Purpose: The herpes simplex virus type 1 thymidine kinase gene(HSV1-tk) is an attractive candidate as a reporter gene in noninvasive reporter gene monitoring system. The HSV1-tk gene was chosen as a reporter gene, because it has been extensively studied, and there are appropriate reporter probes, substrates of HSV1-tk gene product, to apply for HSV1-tk gene imaging. We used radiolabeled 5-iodovinyl-2'-deoxyuridine (IVDU) and 5-iodovinyl-2'-fluoro-2'-deoxyuridine (IVFRU) as reporter probes for HSV1-tk gene monitoring system. Materials and Methods: We prepared HSV1-tk gene transduced Morris hepatoma cell line using retroviral vector, MOLTEN containing HSV1-tk gene. And we confirmed the HSV1-tk gene expression by Northern blotting and Western blotting. We compared in vitro uptakes of radioiodinated IVDU and IVFRU to monitor HSV1-tk gene expression in Morris hepatoma cell line (MCA) and HSV1-tk gene tranduced MCA (MCA-tk) cells until 480 minutes. We also peformed correlation analysis between percentage of HSV1-tk gene tranduced MCA cell % (MCA-tk%) and uptakes of radiolabeled IVDU or IVFRU. Results: MCA-tk cell expressed HSV1-tk mRNA and HSV1-TK protein. Two compounds showed minimal uptake in MCA, but increased uptake was observed in MCA-tk. IVDU showed 4-fold higher accumulation than IVFRU at 480 min in MCA-tk (p<0.01). Both IVDU and IVFRU uptake were linearly correlated ($R^2>0.96$) with increasing MCA-tk%. Conclusion: The radiolabeld IVDU and IVFRU showed higher specific accumulation in retrovirally HSV1-tk gene transfected Morris hepatoma cell line. Both IVDU and IVFRU could be used as good substrates for evaluation of HSV1-tk gene expression.

• The Journal of Small Business Innovation
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• v.20 no.1
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• pp.35-46
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• 2017

The purpose of this study is to obtain the implications for establishing a marketing strategy for retailers by analyzing whether the consumer's preference for products (food) is influenced by on-line, off-line, and omni-channel preferences. The research model consisted of the effects of consumers' food selection characteristics on business preference and omni channel preference. The results of analysis based on 477 questionnaires are summarized as follows. First, food purchasing characteristics that utilize the seven characteristics of (1) freshness of food, (2) taste, (3) quality and safety, (4) tasting, (5) food function and information, (6) country of origin, and (7) brand loyalty, has various effects on types of business preference, omni-channel preference. The adopted hypotheses were selected in order of brand loyalty (5 times), freshness (3 times), tasting (3 times), and origin (1 time). The selective attributes on foods are: (1) what kind of brand, (2) how good the freshness is, (3) tasting. (4) The country of origin is to be compressed. Actually consumers seem to utilize only the core selective factors that are appropriate to the characteristics of the distribution channel rather than all of them in the shopping of the food, and there is three characteristics on Convenience Store and only one or two factors in the other types of business. Second, in the analysis of the omni-channel preference analysis, food selection characteristics has been shown to have a limited effect, which is attributed to the fact that the consumer has not yet understood the concept of omni channel. Third, the results of this study suggest that there is a need to reflect the selection characteristics of foods that have diverse influences by business type in the marketing strategies. Because consumers make reasonable consumption to use both on-line and off-line simultaneously, consumers who use Omni Channel focus on quality and safety of food, identify products through tasting, pursue brands that can trust anywhere. Customers who pursue rationality will have a high preference for using Omni Channel. Product choice characteristic has significant effect on omni-channel preference. For a few years, omni-channel strategy of retailers meets with a difficulty. This study make a first attempt to study omni-channel preference changing retailing paradigm.

• Biotechnology and Bioprocess Engineering:BBE
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• v.8 no.6
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• pp.338-348
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• 2003

In the past decade, considerable progress has been made in developing the appropriate biotechnology for microalgal mass cultivation aimed at establishing a new agro-industry. This review points out the main biological constraints affecting algal biotechnology outdoors and the requirements for making this biotechnology economically viable. One of them is the availability of a wide variety of algal species and improved strains that favorably respond to varying environmental conditions existing outdoors. It is thus just a matter of time and effort before a new methodology like genetic engineering can and will be applied in this field as well. The study of stress physiology and adaptation of microalgae has also an important application in further development of the biotechnology for mass culturing of microalgae. In outdoor cultures, cells are exposed to severe changes in light and temperature much faster than the time scale re-quired for the cells to acclimate. A better understanding of those parameters and the ability to rapidly monitor those conditions will provide the growers with a better knowledge on how to optimize growth and productivity. Induction of accumulation of high value products is associated with stress conditions. Understanding the physiological response may help in providing a better production system for the desired product and, at a later stage, give an insight of the potential for genetic modification of desired strains. The potential use of microalgae as part of a biological system for bioremediation/detoxification and wastewater treatment is also associated with growing the cells under stress conditions. Important developments in monitoring and feedback control of the culture behavior through application of on-line chlorophyll fluorescence technique are in progress. Understanding the process associated with those unique environmental conditions may help in choosing the right culture conditions as well as selecting strains in order to improve the efficiency of the biological process.

• Journal of Physiology & Pathology in Korean Medicine
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• v.17 no.1
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• pp.37-43
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• 2003

Dam-eum(痰飮) is peculiar pathological product which is caused by functional difficulty in human fluid replacement and that principally originated functional difficulty between Lung(肺), Spleen(脾), Kidney(腎) and Sam-Cho(三焦). Dam-eum(痰飮) comes into force to disorder about ascending, decending, in and out of human energy circulation and frequently raises functional difficulty in human fluid replacement. As a result of that, it has much effect on action of Spirit(神志). After literatual study on the effect of Dam-eum(痰飮) on Spirit(神志), the result were obtained as follows : Dam-eum is not affecting to Spirit by itself. When Dam-eum is Positive Symptom(實證), Yang Symptom(陽證), mainly it affects to Spirit with Pung(風), Hwa(火), Kyung(驚), Youl(熱). And when it is Negative Symptom(虛症), it mostly affects with 脾胃虛寒, 心血不足. Dam-eum is a factor of causing disharmony between Heart & Kidney(心腎), Heart & Liver(心肝), Stomach & Gall Bladder Line(胃膽經) by 痰迷心竅, 痰火擾心. So it brings out troubles of fluid replacement. These are the main circumstances of affecting Spirit. To cure Disease of Spirit(神志病), which is caused by Dam-eum, there are not only uses the treatment of 理痰氣, 豁痰開竅. There are two treatments to use together. The first treatment is 淸熱, 定驚, 息風, 淸心 that removes fitted toxin. And the other treatment is that keeps the balance of Internal Organs.

• Proceedings of the KSR Conference
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• 2011.10a
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• pp.3046-3051
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• 2011

The third-rail system is an important device supplying power directly to the Maglev train through physical contact with the collecting shoe. It is directly related to safety and reliability for the running of Maglev. However, most the third-rail system used in Korea depend on foreign product or technologies, Korea Urban Maglev in the development of appropriate power feeding is urgent. In particular, the turnout section is the weakness point in the system because bending force by turnout section movement and fatigue caused by repetitive motion as well as the expansion by temperature, the forces by Maglev collecting shoe is added th the third-rail. Therefore, this paper proposes the third-rail system appropriate for Korean Urban Maglev of turnout section. To verify the structural stability of POSCO ICT third-rail system, the finite element analysis and physical testing was performed. The third-rail is fixed on each side of the turn-out section steel structure by epoxy insulation supporter and the integral behaviors are occurred. Therefore, the maximum horizontal displacements of each support are investigated and then, it is applied to finite element model of the third-rail to investigate the moments and stress. Also, the bending test about one million times and Expansion Joint for the third-rail was performed. The third-rail system safety and reliability was identified by test line on Korea Institute of Machinery & Materials in Deajeon for under the actual usage environment such as the Maglev and turn-out operation.

• The Journal of the Institute of Internet, Broadcasting and Communication
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• v.10 no.6
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• pp.55-62
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• 2010

Monitoring systems are able to report certain events at region of interest(ROI) and to take an appropriate action. From industrial product line full of robots to fire detection, intrusion detection, smart grid application, environmental pollution alarm system, monitoring system has widely used in diverse industry sector. Recently, due to advance of wireless communication technology and availability of low cost sensors, intelligent and/or smart monitoring systems such as sensor networks has been developed. Several deployment methods are introduced to meet various monitoring needs and deployment performance criteria are also summarized to be used to identify weak point and be useful at designing monitoring systems. Both efficiency during deployment and usefulness after the deployment should be assessed. Efficiency factors during deployment are elapsed time, energy required, deployment cost, safety, sensor node failure rate, scalability. Usefulness factors after deployment are ROI coverage, connectivity, uniformity, target density similarity, energy consumption rate per unit time and so on.

• Asian Pacific Journal of Cancer Prevention
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• v.14 no.4
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• pp.2599-2605
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• 2013

The study aimed to evaluate immune-stimulating effects of a well-known Thai folkloric remedy when used for adjuvant therapy with conventional chemotherapeutics for treatment of breast cancer. Immunostimulating influence of the remedy (215 mg/kg body weight per day) on NK cell activity and TNF-${\alpha}$ release from the monocytes/macrophages were investigated in a total of 15 healthy women and 13 female patients with breast cancer (Group 1). The effect of breast tumor surgery on NK cell activity was further investigated in 18 female patients with breast cancer (Group 2). NK cell cytotoxic activity was determined by chromium release cytotoxic assay using K562, an erythroleukemic cell line. TNF-${\alpha}$ release from monocytes/macrophages separated from blood samples was determined through a biological assay using actinomycin D-treated L929 mouse fibroblast cells in the presence and absence of LPS. Baseline NK cell activity of the monocytes/macrophages separated from Group 2 patients expressed as %cytotoxicity was significantly lower than in the healthy subjects at E:T ratios of 100:1 and 25:1. In healthy subjects, there was no change in NK cell cytotoxic activity (%cytotoxicity or LU) following 1 and 2 weeks of treatment with the remedy compared with the baseline at various E:T ratios but the binding activity (%binding) was significantly increased after 2 weeks of treatment. The addition of one or two conventional chemotherapeutic regimens did not significantly reduce the NK cytotoxic activity but did affect release of TNF-${\alpha}$ in both unstimulated and LPS-stimulated samples. Surgery produced a significant suppressive effect on NK cell activity. The use of the remedy as an adjunct therapy may improve therapeutic efficacy and safety profiles of conventional chemotherapeutic regimens through stimulation of the immune system in cancer patients.

• Korean Institute of Interior Design Journal
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• v.23 no.2
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• pp.51-61
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• 2014

Aesthetics of use for modern people can be regarded as being weighted toward universal and rational function since it has focused on an 'automation and efficiency' and 'smartness' in terms of technological and human engineering position. Thus, aesthetics of use as an individual agent has remained in minimal level. This study attempts to seek conceptual model of design regarding the behavior of users from the systematization of critical theories about the usability. To do that, 'HERTZAIN TALES' written by Anthony Dune and the concept and system of Droog Design have been used as a core standard. In line with the various experiments by artist groups such as Fluxus, Memphis group and the work of product semantics, and inherent characteristics of Droog design, which has surfaced from the 1990s, concepts that has mentioned in the Hertzain tales have been organized into main texts. By overcoming cognitive bias inherent in the material culture and by overcoming an existing usability based on immateriality and dialectic solution, the autonomy of critical design and instrumentality of Droog design have been systemized. By interpreting the design aspect of 12 pieces that have been submitted to the RE;MIND section, the core of this study, it has been confirmed that conceptual and critical design's aesthetics of use From the interpretation of the design aspect of 12 pieces that have been submitted to the RE;MIND section, the core of this study, it has been confirmed that conceptual and critical design's aesthetics of use has established the autonomy and independence of the behavior of users, created functional articulation of materials and secured new conceptual model and thus, could be an effective methodical clue for creating an emergent design.

• IE interfaces
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• v.25 no.1
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• pp.21-30
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• 2012

In the past, buyers and suppliers met each other to find common interests off-line in exhibitions and conferences, or through personal connection. These activities were time consuming and costly. With the advent of information era, these activities moved to online market places, where buyers search for suppliers with a set of keywords that are believed to be representative of their requirements. Its fundamental assumption is that all the potential candidates are registered in a certain database. However, recently buyers want to diversify suppliers due to needs of cost competitiveness or frequent new product development. To this end, instead of choosing suppliers from the supplier pool, discovering suppliers from all over the world should be emphasized. In order to enable buyers to describe their requirements and suppliers to capture their manufacturing capabilities via online market places, the semantic differences of terms between buyers and suppliers should be resolved. The paper summarizes various supplier discovery frameworks and prototype systems, which can be employed to expose domestic small-medium enterprises into global buyers in the near future.

• Journal of Family Resource Management and Policy Review
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• v.16 no.1
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• pp.85-101
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• 2012

This study aims to identify the propensity to consume and housing characteristics according to life cycle as the main independent variable. It also tries to understand the consumption power and housing style of each cycle and analyze whether there are differences between each life cycle, ultimately aiming to match the propensity to consume and housing style with each cycle. For empirical analysis, on and off-line surveys of 488 people were collected and analyzed by cross analysis, factor analysis and analysis of variance. The prime research findings are as follows: First of all, factor analysis on propensity to consume showed four main sub-factors as rational consumption, conspicuous consumption, trend consumption, and status consumption. Secondly, current and preferred housing styles ranked from modern, natural, casual, classic and romantic, which proved that people are currently housed in preferred housing styles. Thirdly, in case of housing-related characteristics of life cycles, the size of the house increased as the levels progressed, and then decreased at level 6 when the children grew into adulthood. The majority of the population was paying a monthly rent, a few were leasing, and a very few owned their housing. 58% were living in apartments, but in levels 1-2, more were living in row houses and high-rise residential buildings, while in level 6 it was detached houses. Fourthly, the propensity to consume according to life cycle tended to shift from conspicuous and trend-based consumption to rational consumption as the cycles progressed. Fifthly, the preferred housing styles were modern and natural styles, regardless of life cycle. These study results can be applied to product development and marketing activities based on their accurate analysis of customers' needs, which can thus bring further customer satisfaction.