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Biological Constraints in Algal Biotechnology  

Torzillo, Giuseppe (CNR-Istituto per lo Studio degli Ecosistemi, Sezione di Firenze, Via Madonna del Piano)
Pushparaj, Benjamin (CNR-Istituto per lo Studio degli Ecosistemi, Sezione di Firenze, Via Madonna del Piano)
Masojidek, Jiri (Institute of Physical Biology, University of South Bohemia, Department of Autotrophic Microorganisms, Institute of Microbiology, Academy of Sciences)
Vonshak, Avigad (Microalgal Biotechnology Laboratory, Jacob Blaustein Institute for Desert Research, Ben-Gurion University of the Negev, Sede Boqer Campus)
Publication Information
Biotechnology and Bioprocess Engineering:BBE / v.8, no.6, 2003 , pp. 338-348 More about this Journal
Abstract
In the past decade, considerable progress has been made in developing the appropriate biotechnology for microalgal mass cultivation aimed at establishing a new agro-industry. This review points out the main biological constraints affecting algal biotechnology outdoors and the requirements for making this biotechnology economically viable. One of them is the availability of a wide variety of algal species and improved strains that favorably respond to varying environmental conditions existing outdoors. It is thus just a matter of time and effort before a new methodology like genetic engineering can and will be applied in this field as well. The study of stress physiology and adaptation of microalgae has also an important application in further development of the biotechnology for mass culturing of microalgae. In outdoor cultures, cells are exposed to severe changes in light and temperature much faster than the time scale re-quired for the cells to acclimate. A better understanding of those parameters and the ability to rapidly monitor those conditions will provide the growers with a better knowledge on how to optimize growth and productivity. Induction of accumulation of high value products is associated with stress conditions. Understanding the physiological response may help in providing a better production system for the desired product and, at a later stage, give an insight of the potential for genetic modification of desired strains. The potential use of microalgae as part of a biological system for bioremediation/detoxification and wastewater treatment is also associated with growing the cells under stress conditions. Important developments in monitoring and feedback control of the culture behavior through application of on-line chlorophyll fluorescence technique are in progress. Understanding the process associated with those unique environmental conditions may help in choosing the right culture conditions as well as selecting strains in order to improve the efficiency of the biological process.
Keywords
outdoor cultures; phtobioreactors; oxygen stress; photoinhibition; low temperature stress; chlorophyll fluorescence;
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Times Cited By Web Of Science : 14  (Related Records In Web of Science)
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1 Investigation of photobioreactor design for enhancing the photosynthetic productivity of microalgae /
[ Morita,M.;Y.Watanabe;H.Saiki ] / Biotechnol. Bioeng.   DOI   ScienceOn
2 Optimization of microalgae production in a shallow outdoor fiume /
[ Laws,E.A.;T.Satoru;J.Hirata;L.Pang ] / Biotechnol. Bioeng.   DOI   ScienceOn
3 Outdoor algal mass cultures: Ⅱ. Photosynthetic yield limitations /
[ Goldman,J.C. ] / Water Res.   DOI   ScienceOn
4 Low temperature chlorophyll fluorescence in leaves and its relationship to photon yield of phototosynthesis in photoinhibition /
[ Bjorkman,O.;D.J.Kyle(ed.);C.BlOsmond(ed.);C.J.Arntzen(ed.) ] / Photoinhibition
5 Transient analysis and performance studies of two tubular photobioreactors for outdoor culture of Spirulina /
[ Prakash,J.;G.Torzillo;B.Pushparaj;P.Carlozzi;R.Materassi ] / Int. J. Energ. Res.   DOI   ScienceOn
6 tlal, a DNA insertional transformant of the green alga Chlamidomonas reinhardtii with truncated light-harvesting chlorophyll antenna size /
[ Polle,J.E.W.;S.D.Kanakagiri;A.Melis ] / Planta
7 In-situ monitoring of chlorophyll fluorescence to assess the synergistic effect of low temperature and high irradiance stresses in Spirulina cultures grown outdoors in photobioreactors /
[ Torzillo,G.;P.Accolla;E.Pinzani;J.Masojidek ] / J. Appl. Phycol.   DOI   ScienceOn
8 Mechanisms for scavenging reactive molecules generated in chloroplasts under light stress /
[ Adada,K.;N.r.Baker(ed.);J.R.Bowyer(ed.) ] / Photoinhibition of Photosynthesis: From Molecular Mechanisms to the Field
9 A closed solar photobioreactor for cultivation of microalgae under supra-high irradiance: Basic design and performance /
[ Masojidek,J.;S.Papacek;M.Sergejevova;V.Jirka;J.Cerveny;J.Kunc;J.Korencko;O.Verbovikova;J.Kopecky;J.Stys;G.Torzillo ] / J. Appl. Phycol.   DOI   ScienceOn
10 Photoautotrophic bioreactor using visible solar rays condensed by Fresnel lenses and transmitted through optical fibers /
[ Mori,K. ] / Biotechnol. Bioeng. Symp.
11 Effect of hydrodynamic stress on Dunaliella growth /
[ Silva,H.J.;T.Cortinas;R.J.Ertola ] / J. Chem. Technol. Biotechnol.   DOI   ScienceOn
12 /
[ Bird,R.B.;W.E.Stewart;E.N.Lightfoot ] / Transport Phenomena
13 Tubular photobioreactor design for algal cultures /
[ Grima,E.M.;F.G.A.Fernandez;Y.Chisti ] / J. Biotechnol.   DOI   ScienceOn
14 Photosystem-Ⅱ repair and chloroplast recovery from irradiance stress: Relationship between chronic photoinhibition, light-harvesting chlorophyll antenna size and photosynthetic productivity in Dunaliella salina (green algae) /
[ Neidhardt,J.;J.R.Benemann;L.Zhang;A.Melis ] / Photosynth. Res.   DOI   ScienceOn
15 Involvement of zeaxanthin and of the Cbr protein in the repair of photosystem Ⅱ from photoinhibition in the green alga Dunaliella salina /
[ Jin,E.S.;E.W.;E.S.Jin;J.E.W.Polle;A.Melis ] / Biochim. Biophys. Acta   DOI   ScienceOn
16 Bioreactors /
[ Torzillo,G.;A.Vonshak(ed.) ] / Spirulina platensis (Arthrospira): Physiology, Cell-biology and Biotechnology
17 Energy balance and acclimation to light and cold /
[ Huner,N.P.A.;G.Oquist;F.Sarhan ] / Trends Plant Sci.   DOI   ScienceOn
18 Solar fiber-optic mini-dishes: A new approach to the efficient collection of sunlight /
[ Feuermann,D.;J.M.Gordon ] / Solar Energy   DOI   ScienceOn
19 Light and oxygen stress in Spirulina platensis (Cyanobacteria) grown outdoors in tubular reactors /
[ Vonshak,A.;G.Torzillo;P.Accolla;L.Tomaselli ] / Physiol. Plant.   DOI
20 A real-life, large scale and fully controlled photobioreactor for microalgae production /
[ Fornari,L.F.;F.L.Lupoli;A.C.Carella;G.V.G.V.Gregorini ] / Abstracts of 8th International Conference on Applied Algology
21 An integrated solar and artificial light system for internal illumination of photobioreactors /
[ Ogbonna,J.C.;T.Soejima;H.Tanaka ] / J. Biotechnol.   DOI   ScienceOn
22 Light intensity regulation of cab gene transcription is signaled by the redox state of the PQ pool /
[ Ecoubas,J.M.;M.Lomas;J.LaRoche;P.G.Falkowski ] / Proc. Natl. Acad. Sci.   DOI   ScienceOn
23 An integrated culture system for outdoor productin of microalgae and cyanobacteria /
[ Pushparaj,B.;E.Pelosi;M.R.Tredii;E.Pinzani;R.Materassi ] / J. Appl. Phycol.   DOI   ScienceOn
24 Cellfragility: The key problem of microalgae mass production in closed photobioreactors /
[ Gudin,C.;D.Chaumont ] / Biores. Technol.   DOI   ScienceOn
25 A tubular bioreactor for photosynthetic production of biomass from carbon dioxide: Design and performance /
[ Pirt,S.J.;Y.K.Lee;M.R.Walach;M.W.Pirt;H.H.M.Balyuzi;M.J.Bazin ] / J. Chem. Biotechnol.
26 Photoadaptation, phoinhibition and productivity in the blue-green alga Spirulina platensis grown outdoors /
[ Vonshak,A.;R.Guy ] / Plant Cell Environ.   DOI
27 Productivity of Spirulina in a strongly curved outdoor tubular photobioreactor /
[ Carlozzi,P.;G.Torzillo ] / Appl. Microbiol. Biotechnol.   DOI   ScienceOn
28 Quantitative assessment of the major limitations on productivity of Spirulina platensis in open raceways /
[ Richmodn,A.;E.Lichtenberg;B.Stahl;A.Vonshak ] / J. Appl. Phycol.   DOI
29 Inhibitory effect of oxygen accumulation on the growth of Spirulina platensis /
[ Markez,F.J.;K.Sasaki;N.Nishio;S.Nagai ] / Biotechnol. Lett.
30 Online monitoring of chlorophyll fluorescence to assess the extent of photoinhibition of photosynthesis induced by high oxygen concentration and low temperature and its effect on the productivity of outdoor cultures of Spirulina platensis (Cyanobacteria) /
[ Torzillo,G.;P.Bernardini;J.Masojidek ] / J. Phycol.   DOI   ScienceOn
31 A simple algal production system designed to utilize the flashing light effect /
[ Laws,E.A.;K.L.Terry;J.Wickman;M.S.Chalup ] / Biotechnol. Bioeng.   DOI   ScienceOn
32 Dunaliella salina (Chlorophyta) with small chlorophyll antenna sizes exhibit photosynthetic productivities and photon use efficiencies than normally pigmented cells /
[ Melis,A.;J.Neidhardt;J.R.Benemann ] / J. Appl. Phycol.
33 Sub-optimal morning temperature induces photoihibition in dense outdoor cultures of the alga Monodus subterraneus (Eustigmatophyta) /
[ Vonshak,A.;G.Torzillo;J.Masojidek;S.Boussiba ] / Plant Cell Environ.   DOI   ScienceOn
34 Shear sensitivity /
[ Chisti,Y.;M.C.Flickinger(ed.);S.W.Drew(ed.) ] / Encyclopedia of Bioprocess Technology: Fermentation, Biocatalysis and Bioseparation
35 Algal photoinhibition and photosynthesis in the aquatic environment /
[ Neale,J.;D.J.Kyle(ed.);C.B.Osmond(ed.);C.J.Arntzen(ed.) ] / Photoinhibition
36 Photoinhibition of photosynthesis in nature /
[ Long,S.P.;S.Humphiries;G.P.Falkowski ] / Annu. Rev. Plant Physiol. Plant Biol.
37 The effect of reducing light-harvesting pigments onmarine microalgal productivity /
[ Nakajima,Y.;R.Ueda ] / J. Appl. Phycol.   DOI   ScienceOn
38 Current status of the large-scale culture of algae /
[ Burlew,J.S.;J.S.Burlew(ed.) ] / Algal Culture: Form Laboratory to Pilot Plant
39 Swirling flow implementation in photobioreactor as a potential means of improving the microalgae culture yields /
[ Muller-Feuga,A.;R.Le Guedes;J.Pruvost;P.Legentilhomme;J.Legrand ] / Abstracts of the 9th International Conference on Applied Algology
40 Use of concentric-tube airlift ohotobioreactors for microalgal outdoor mass cultures /
[ Camacho,G.F.;A.C.Gomez;F.G.A.Fernadez;J.F.Sevilla;E.M.Grima ] / Enzyme Microb. Technol.   DOI   ScienceOn
41 A new tubular reactor for mass production of microalgae outdoors /
[ Richmond,A.;S.Boussiba;A.Vonshak;R.Kopel ] / J. Appl. Phycol.   DOI   ScienceOn
42 Improvement of photosynthesis in dense microalgal suspension by reduction of light harvesting pigments /
[ Nakajima,Y.;R.Ueda ] / J. Appl. Phycol.
43 Spirulina culture in Israel /
[ Richmodn,A.;A.Vonshak ] / Arch. Hydrobiol. Beith. Erg. Limnol.
44 The relationship between the quantum yield of photosynthesis electron transport and quenching of chlorophyll fluorescence /
[ Genty,B.;J.M.Briantais;N.R.Baker ] / Biochim. Biophys. Acta
45 Photoinhibitoruy stress induced by high oxygen and low temperature in outdoor clutures of Arthrospira platensis grown in closed photobioreactors /
[ Torzillo,G.;J.Komenda;J.Kopecky;C.Faraloni;J.Masojidek ] / Abstracts of Third European Phycological Congress
46 Physiological characteristics of Spirulina platensis (Cyanobacteria) cultured at ultrahigh cell densities /
[ Hu,Q.;H.Guterman;A.Richmond ] / J. Phycol.   DOI   ScienceOn
47 Improvement of mass transfer characteristics and productivities of inclined tubular photobioreactors by installation of internal static mixers /
[ Ugwu,C.U.;J.C.Ogbonna;H.Tanaka ] / Appl. Microbiol. Biotechnol.   DOI   ScienceOn
48 Optimal tilt angles of enclosed reactors for growing photoautotrophic microorganisms outdoors /
[ Hu,Q.;M.Faiman;A.Richmond ] / J. Ferment. Bioeng.   DOI   ScienceOn
49 How power supply affects microalgal growth /
[ Allias,B.;C.M.G.Lopez;F.G.A.Fernandez;J.M.F.Sevilla;J.L.G.Sanchez;E.M.Grima ] / Abstracts of 5th European Workshop
50 CO₂ and O₂ interaction in photoinhibition /
[ Krause,G.H.;G.Cornic;D.J.Kyle(ed.);C.B.Osmond(ed.);C.J.Arntzen(ed.) ] / Photoinhibition
51 Scale-up of tubular photobioreactors /
[ Grima,E.M.;F.G.A.Fernandez;F.G.Camaho;F.C.Rubio;Y.Chisti ] / J. Appl. Phycol.   DOI   ScienceOn
52 Glutamate production from CO₂ by marine cyanobacterium Synechococcus sp. using a novel biosolar reactor employing light diffusing optical fibers /
[ Matsunaga,T.;H.Takeyama;H.Sudo;N.Oyama;S.Ariura;H.Takano;H.Hirano;J.G.Burgess;K.Sode;N.Nakamura ] / Appl. Biochem. Biotechnol.
53 Use of chlorophyll fluorescence to estimate the effect of photoinhibition in outdoor cultures of Spirulina platensis /
[ Vonshak,A.;G.Torzillo;L.Tomaselli ] / J. Appl. Phycol.   DOI   ScienceOn
54 Compensatory changes in photosystem Ⅱ electron turnover rates protect phtosynthesis from photoinhibition /
[ Behrenfeld,M.J.;O.Prasil;Z.S.Kolber;M.Babin;P.G.Falkowski ] / Photosynth. Res.   DOI   ScienceOn
55 A. Photoinhibition in outdoor Spirulina platensis cultures assessed by polyphasic chlorophyll fluorescence transients /
[ Lu,C.M.;A.Vonshak ] / J. Appl. Phycol.   DOI   ScienceOn
56 Effect of oxygen concentration on the protein content of Spirulina biomass /
[ Torzillo,G.;L.Giovannetti;F.Bocci;R.Materassi ] / Biotechnol. Bioeng.   DOI   ScienceOn
57 /
[ Hager,A. ] / Pigments in Plants.
58 Design and performance of an α-type tubular photobioreactor for mass cultivation of microalgae /
[ Lee,Y.K.;S.Y.Ding;C.S.Low;Y.C.Chang;W.L.Forday;P.C.Chew ] / J. Appl. Phycol.   DOI   ScienceOn
59 Environmental effects on algal photosynthesis: temperature /
[ Davison,I.R. ] / J. Phycol.   DOI
60 The future of microalgal biotechnology /
[ Benemann,J.R.;R.D.Cressewell(ed.);T.A.V.Rees(ed.);N.Shah(ed.) ] / Algal and Cyanobacterial Technology
61 Enclosed outdoor photobioreactors: light regime, photosynthetic efficiency, scale-up, and future prospects /
[ Janssen,M.;J.Tramper;L.R.Mur;R.H.Wijffels ] / Biotechnol. Bioeng.   DOI   ScienceOn
62 Biological limitations in developing the biotechnology of algal mass cultivation /
[ Vonshak,A. ] / SC. de L'eau
63 A two-plane photobioreactor for outdoor culture of Spirulina /
[ Torzillo,G.;P.Carlozzi;B.Pushparaj;E.Montaini;R.Materassi ] / Biotechnol. Bioeng.   DOI   ScienceOn
64 Dilution of solar radiation through "culture" lamination in photobioreactor rows facing southnorth: A way to improve the efficiency of light utilization by cyanobacteria (Arthrospira platensis) /
[ Carlozzi,P. ] / Biotechnol. Bioeng.
65 Carbon dioxide fixation in bath culture of Chlorella sp.using a photobioreactor with sunlight coolection device /
[ Hirata,S.;M.Hayashitani;M.taya;S.Tone ] / J. Ferment. Bioeng.   DOI   ScienceOn
66 Evaluation of photobioreactor heat balance for predicting changes in culture medium temperature due to light irradiation /
[ Morita,M.;Y.Watanabe;H.Saiki ] / Biotechnol. Bioeng.   DOI   ScienceOn
67 Evaluation of photosynthetic efficiency in microalgal cultures using averaged irradiance /
[ Grima,E.M.;F.G.Camacho;J.A.S.Peres;F.G.A.Fernandez;J.M.F.Sevilla ] / Enzyme Microb. Technol.   DOI   ScienceOn