• Toxicological Research
• /
• v.34 no.1
• /
• pp.1-6
• /
• 2018

The purpose of this study was to detect cell death in the liver of mice treated with thioacetamide (TAA) using fluorescence bioimaging and compare this outcome with that using conventional histopathological examination. At 6 weeks of age, 24 mice were randomly divided into three groups: group 1 (G1), control group; group 2 (G2), fluorescence probe control group; group 3 (G3), TAA-treated group. G3 mice were treated with TAA. Twenty-two hours after TAA treatment, G2 and G3 mice were treated with Annexin-Vivo 750. Fluorescence in vivo bioimaging was performed by fluorescence molecular tomography at two hours after Annexin-Vivo 750 treatment, and fluorescence ex vivo bioimaging of the liver was performed. Liver damage was validated by histopathological examination. In vivo bioimaging showed that the fluorescence intensity was increased in the right upper part of G3 mice compared with that in G2 mice, whereas G1 mice showed no signal. Additionally ex vivo bioimaging showed that the fluorescence intensity was significantly increased in the livers of G3 mice compared with those in G1 or G2 mice (p < 0.05). Histopathological examination of the liver showed no cell death in G1 and G2 mice. However, in G3 mice, there was destruction of hepatocytes and increased cell death. Terminal deoxynucleotidyl transferase dUTP nick end labeling staining confirmed many cell death features in the liver of G3 mice, whereas no pathological findings were observed in the liver of G1 and G2 mice. Taken together, fluorescence bioimaging in this study showed the detection of cell death and made it possible to quantify the level of cell death in male mice. The outcome was correlated with conventional biomedical examination. As it was difficult to differentiate histological location by fluorescent bioimaging, it is necessary to develop specific fluorescent dyes for monitoring hepatic disease progression and to exploit new bioimaging techniques without dye-labeling.

• Journal of Korea Multimedia Society
• /
• v.17 no.2
• /
• pp.152-159
• /
• 2014

Recently. utilization of remote sensing exploration and cloud computing has been extended to efficient measurement, store, and update of bathymetry map data according to cloud computing technology. In the field of real ocean, water depth measurements and measurement data management, distribution, and display equipment for the development and dissemination have generated a lot of time and cost. To improve these problems, through real-time three-dimensional display system at this location, we can determine the importance of measurement activities, and reduce the time and cost of measurement activities. Data measured from marine probe vessels and remote sensing exploration equipments and other various channels can be handled and managed. In this paper, we propose a realtime three-dimensional display system through the depth measurements from remote sensing exploration. The proposed real-time three-dimensional display system can be effectively applied in the field of measurement of the topographical survey of the land as well as bathymetry of the sea.

• Journal of the Korean Society for Nondestructive Testing
• /
• v.16 no.1
• /
• pp.29-33
• /
• 1996

The eddy current test(ECT) probe of differential encircling coil type was designed and fabricated, and the optimum condition of ECT was derived for the examination of the irradiated fuel rod. The correlation between ECT test frequency and phase & amplitude was derived by performing the test of the standard rig that includes inner notches, outer notches and through-holes. The defect of through-hole was predicted by ECT at the G33-N2 fuel rod irradiated in the Kori-1 nuclear power reactor. The metallographic examination on the G33-N2 fuel rod was Performed at the defect location predicted by ECT. The result of metallographic examination for the G33-N2 fuel rod was in good agreement with that of ECT. This proves that the evaluation for integrity of irradiated fuel rod by ECT is reliable.

• 유체기계공업학회:학술대회논문집
• /
• 2006.08a
• /
• pp.187-190
• /
• 2006

In the present study, effects of tree-stream turbulence and surface trip wire on the flow past a sphere at $Re\;=\;0.4\;{\times}\;10^5\;{\sim}\;2.8\;{\times}\;10^5$ are investigated through wind tunnel experiments. Various types of grids are installed upstream of the sphere in order to change the tree-stream turbulence intensity. In the case of surface trip wire, 0.5mm and 2mm trip wires are attached from $20^{\circ}\;{\sim}\;90^{\circ}$ at $10^{\circ}$ interval along the streamwise direction. To investigate the flow around a sphere, drag measurement using a load cell, surface-pressure measurement, surface visualization using oil-flow pattern and near-wall velocity measurement using an I-type hot-wire probe are conducted. In the variation of free-stream turbulence, the critical Reynolds number decreases and drag crisis occurs earlier with increasing turbulence intensity. With increasing Reynolds number, the laminar separation point moves downstream, but the reattachment point after laminar separation and the main separation point are fixed, resulting in constant drag coefficient at each free-stream turbulence intensity. At the supercritical regime, as Reynolds number is further increased, the separation bubble is regressed but the reattachment and the main separation points are fixed. In the case of surface trip wire directly disturbing the boundary layer flow, the critical Reynolds number decreases further with trip wire located more downstream. However, the drag coefficient after drag crisis remains constant irrespective of the trip location.

• Journal of the Korean Society for Nondestructive Testing
• /
• v.30 no.4
• /
• pp.295-301
• /
• 2010

Flow accelerated corrosion(FAC) of carbon steel pipes in nuclear power plants has been known as one of the major degradation mechanisms. It could have bad influence on the plant reliability and safety. Also detection of FAC is a significant cost to the nuclear power plant because of the need to remove and replace insulation. Recently, the interest of the guided wave testing(GWT) has grown because it allows long range inspection without removing insulation of the pipe except at the probe position. If GWT can be applied to detection of FAC damages, it will can significantly reduce the cost for the inspection of the pipes. The objective of this study was to determine the capability of GWT to identify location of FAC damages. In this paper, three kinds of techniques were used to measure the amplitude ratio between the first and the second welds at the elbow area of mock-ups that contain real FAC damages. As a result, optimal inspection technique and minimum detectability to detect FAC damages drew a conclusion.

• Korean Journal of Optics and Photonics
• /
• v.13 no.6
• /
• pp.521-529
• /
• 2002

We have recently proposed the new concept of a phase-measuring volumetric interferometer that enables us to accurately measure the xyz-coordinates of the probe without metrology frames. The interferometer is composed of a movable target and a fixed photo-detector array. The target is made of point diffraction sources to emit two spherical wavefronts, whose interference is monitored by an array of photo-detectors. Phase shifting is applied to obtain the precise phase values of the photo-detectors. Then the measured phases are fitted to a geometric model of multilateration so as to determine the xyz-location of the target by minimizing least square errors. The proposed interferometer has been designed and built with a volumetric uncertainty of less than 1.0 $\mu\textrm{m}$ within a cubic working volume of side 120 mm. Here, in this paper, we also present error sources, an evaluated uncertainty, and test results from the prototype system. The self-calibration of two-dimensional precision metrology stages is applied to test the performance of the interferometer.

• Korean Journal of Optics and Photonics
• /
• v.17 no.3
• /
• pp.223-230
• /
• 2006

We demonstrate a distributed fiber sensor system based on spatially-selective Brillouin scattering, using a single laser diode as a light source whose optical frequency is directly modulated by the injection current. The pump and the counter-propagating probe lights, which are sinusoidally frequency-modulated, are superposed in the fiber so that stimulated Brillouin scattering takes places only at a specific location along the fiber. Brillouin gain peak position is controlled by varying the modulation frequency. Distributions of Brillouin shift frequency are measured for the case of concatenated optical fibers of two different kinds and also for the case of temperature distribution. The temperature coefficient of the Brillouin shift frequency is measured to be $1.33MHz/^{\circ}C$.

• Journal of Korean Neurosurgical Society
• /
• v.55 no.3
• /
• pp.131-135
• /
• 2014

Objective : With the growing interests of bacteria as a targeting vector for cancer treatment, diverse genetically engineered Salmonella has been reported to be capable of targeting primary or metastatic tumor regions after intravenous injection into mouse tumor models. The purpose of this study was to investigate the capability of the genetically engineered Salmonella typhimurium (S. typhimurium) to access the glioma xenograft, which was monitored in mouse brain tumor models using optical bioluminescence imaging technique. Methods : U87 malignant glioma cells (U87-MG) stably transfected with firefly luciferase (Fluc) were implanted into BALB/cAnN nude mice by stereotactic injection into the striatum. After tumor formation, attenuated S. typhimurium expressing bacterial luciferase (Lux) was injected into the tail vein. Bioluminescence signals from transfected cells or bacteria were monitored using a cooled charge-coupled device camera to identify the tumor location or to trace the bacterial migration. Immunofluorescence staining was also performed in frozen sections of mouse glioma xenograft. Results : The injected S. typhimurium exclusively localized in the glioma xenograft region of U87-MG-bearing mouse. Immunofluorescence staining also demonstrated the accumulation of S. typhimurium in the brain tumors. Conclusion : The present study demonstrated that S. typhimurium can target glioma xenograft, and may provide a potentially therapeutic probe for glioma.

• Journal of Microbiology and Biotechnology
• /
• v.32 no.10
• /
• pp.1344-1354
• /
• 2022

Laryngeal cancer is one of the highest incidence, most prevalently diagnosed head and neck cancers, making it critically necessary to probe effective targets for laryngeal cancer treatment. Here, real-time quantitative reverse transcription PCR (qRT-PCR) and western blot analysis were used to detect gene expression levels in laryngeal cancer cell lines. Fluorescence in situ hybridization (FISH) and subcellular fractionation assays were used to detect the subcellular location. Functional assays encompassing Cell Counting Kit-8 (CCK-8), 5-ethynyl-2'-deoxyuridine (EdU), transwell and wound healing assays were performed to examine the effects of target genes on cell proliferation and migration in laryngeal cancer. The in vivo effects were proved by animal experiments. RNA-binding protein immunoprecipitation (RIP), RNA pulldown and luciferase reporter assays were used to investigate the underlying regulatory mechanisms. The results showed that KIF26B antisense RNA 1 (KIF26B-AS1) propels cell proliferation and migration in laryngeal cancer and regulates the toll-like receptor 4 (TLR4) signaling pathway. KIF26B-AS1 also recruits FUS to stabilize TLR4 mRNA, consequently activating the TLR4 signaling pathway. Furthermore, KIF26B-AS1 plays an oncogenic role in laryngeal cancer via upregulating TLR4 expression as well as the FUS/TLR4 pathway axis, findings which offer novel insight for targeted therapies in the treatment of laryngeal cancer patients.

• The Bulletin of The Korean Astronomical Society
• /
• v.46 no.1
• /
• pp.54.4-55
• /
• 2021

The Seoul National University AGN Monitoring Project (SAMP) is a welldesigned long-term AGN reverberation mapping project. SAMP focuses on the luminous AGNs out to z~0.5 with relative long time lags between AGN continuum and broad emission lines and aims to probe the high-end of the AGN broad line region (BLR) size-luminosity (R-L) relation. The pilot observations started in October 2015 for 100 AGNs to confirm the variability and the H and [O III] emission line strengths. Based on the initial variability test, 48 quasars has been continued spectroscopic monitoring since Feb. 2016 with Lick 3m and MDM 2.4m telescopes with a cadence of ~20 days. Supporting photometric monitoring in B and V band was conducted at multiple facilities including the MDM 1.3m, LOAO, and DOAO telescopes with a cadence of ~10 days. By the time of Feb. 2021, we have obtained five years spectroscopic and photometric data. More than 30 AGNs shows significant variability in five-year baseline and 16 of them show well detected lags between B-band and H. Here, we report some examples of SAMP light curves and lag detections using the first five-year data as well as the location of our 16 targets in the AGN BLR R-L relation. These measurements are consistent with the existing R-L relation and located at the high-end. With the coming data, SAMP are hopefully to report more AGNs with well detected lags. Our results demonstrate the general feasibility and potential of long-term reverberation project with medium cadence for luminous AGNs.