Browse > Article
http://dx.doi.org/10.3340/jkns.2014.55.3.131

Targeting Orthotopic Glioma in Mice with Genetically Engineered Salmonella typhimurium  

Wen, Min (Brain Tumor Research Laboratory and Department of Neurosurgery, Chonnam National University Research Institute, Chonnam National University Hwasun Hospital and Medical School)
Jung, Shin (Brain Tumor Research Laboratory and Department of Neurosurgery, Chonnam National University Research Institute, Chonnam National University Hwasun Hospital and Medical School)
Moon, Kyung-Sub (Brain Tumor Research Laboratory and Department of Neurosurgery, Chonnam National University Research Institute, Chonnam National University Hwasun Hospital and Medical School)
Jiang, Shen Nan (Laboratory of In Vivo Molecular Imaging (LOVMI) and Department of Nuclear Medicine, Chonnam National University Research Institute, Chonnam National University Hwasun Hospital and Medical School)
Li, Song-Yuan (Brain Tumor Research Laboratory and Department of Neurosurgery, Chonnam National University Research Institute, Chonnam National University Hwasun Hospital and Medical School)
Min, Jung-Joon (Laboratory of In Vivo Molecular Imaging (LOVMI) and Department of Nuclear Medicine, Chonnam National University Research Institute, Chonnam National University Hwasun Hospital and Medical School)
Publication Information
Journal of Korean Neurosurgical Society / v.55, no.3, 2014 , pp. 131-135 More about this Journal
Abstract
Objective : With the growing interests of bacteria as a targeting vector for cancer treatment, diverse genetically engineered Salmonella has been reported to be capable of targeting primary or metastatic tumor regions after intravenous injection into mouse tumor models. The purpose of this study was to investigate the capability of the genetically engineered Salmonella typhimurium (S. typhimurium) to access the glioma xenograft, which was monitored in mouse brain tumor models using optical bioluminescence imaging technique. Methods : U87 malignant glioma cells (U87-MG) stably transfected with firefly luciferase (Fluc) were implanted into BALB/cAnN nude mice by stereotactic injection into the striatum. After tumor formation, attenuated S. typhimurium expressing bacterial luciferase (Lux) was injected into the tail vein. Bioluminescence signals from transfected cells or bacteria were monitored using a cooled charge-coupled device camera to identify the tumor location or to trace the bacterial migration. Immunofluorescence staining was also performed in frozen sections of mouse glioma xenograft. Results : The injected S. typhimurium exclusively localized in the glioma xenograft region of U87-MG-bearing mouse. Immunofluorescence staining also demonstrated the accumulation of S. typhimurium in the brain tumors. Conclusion : The present study demonstrated that S. typhimurium can target glioma xenograft, and may provide a potentially therapeutic probe for glioma.
Keywords
Cancer targeting; Mouse glioma model; Optical bioluminescence imaging; Salmonella typhimurium; U87-MG;
Citations & Related Records
연도 인용수 순위
  • Reference
1 Yu YA, Shabahang S, Timiryasova TM, Zhang Q, Beltz R, Gentschev I, et al. : Visualization of tumors and metastases in live animals with bacteria and vaccinia virus encoding light-emitting proteins. Nat Biotechnol 22 : 313-320, 2004   DOI   ScienceOn
2 Zhao M, Geller J, Ma H, Yang M, Penman S, Hoffman RM : Monotherapy with a tumor-targeting mutant of Salmonella typhimurium cures orthotopic metastatic mouse models of human prostate cancer. Proc Natl Acad Sci U S A 104 : 10170-10174, 2007   DOI   ScienceOn
3 Zhao M, Yang M, Li XM, Jiang P, Baranov E, Li S, et al. : Tumor-targeting bacterial therapy with amino acid auxotrophs of GFP-expressing Salmonella typhimurium. Proc Natl Acad Sci U S A 102 : 755-760, 2005   DOI   ScienceOn
4 Min JJ, Nguyen VH, Kim HJ, Hong Y, Choy HE : Quantitative bioluminescence imaging of tumor-targeting bacteria in living animals. Nat Protoc 3 : 629-636, 2008   DOI   ScienceOn
5 Momiyama M, Zhao M, Kimura H, Tran B, Chishima T, Bouvet M, et al. : Inhibition and eradication of human glioma with tumor-targeting Salmonella typhimurium in an orthotopic nude-mouse model. Cell Cycle 11 : 628-632, 2012   DOI
6 Nguyen VH, Kim HS, Ha JM, Hong Y, Choy HE, Min JJ : Genetically engineered Salmonella typhimurium as an imageable therapeutic probe for cancer. Cancer Res 70 : 18-23, 2010   DOI
7 Pawelek JM, Low KB, Bermudes D : Bacteria as tumour-targeting vectors. Lancet Oncol 4 : 548-556, 2003   DOI   ScienceOn
8 Pawelek JM, Low KB, Bermudes D : Tumor-targeted Salmonella as a novel anticancer vector. Cancer Res 57 : 4537-4544, 1997
9 Rehemtulla A, Stegman LD, Cardozo SJ, Gupta S, Hall DE, Contag CH, et al. : Rapid and quantitative assessment of cancer treatment response using in vivo bioluminescence imaging. Neoplasia 2 : 491-495, 2000   DOI   ScienceOn
10 Szentirmai O, Baker CH, Lin N, Szucs S, Takahashi M, Kiryu S, et al. : Noninvasive bioluminescence imaging of luciferase expressing intracranial U87 xenografts : correlation with magnetic resonance imaging determined tumor volume and longitudinal use in assessing tumor growth and antiangiogenic treatment effect. Neurosurgery 58 : 365-372; discussion 365-372, 2006   DOI
11 Tang Y, Shah K, Messerli SM, Snyder E, Breakefield X, Weissleder R : In vivo tracking of neural progenitor cell migration to glioblastomas. Hum Gene Ther 14 : 1247-1254, 2003   DOI   ScienceOn
12 Dinca EB, Voicu RV, Ciurea AV : Bioluminescence imaging of invasive intracranial xenografts : implications for translational research and targeted therapeutics of brain tumors. Neurosurg Rev 33 : 385-394, 2010   DOI
13 Hoffman S, Propp JM, McCarthy BJ : Temporal trends in incidence of primary brain tumors in the United States, 1985-1999. Neuro Oncol 8 : 27-37, 2006   DOI   ScienceOn
14 Min JJ, Kim HJ, Park JH, Moon S, Jeong JH, Hong YJ, et al. : Noninvasive real-time imaging of tumors and metastases using tumor-targeting light-emitting Escherichia coli. Mol Imaging Biol 10 : 54-61, 2008   DOI   ScienceOn